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6.3.1.2: glutamine synthetase

This is an abbreviated version!
For detailed information about glutamine synthetase, go to the full flat file.

Word Map on EC 6.3.1.2

Reaction

ATP
+
L-glutamate
 +
NH3
=
ADP
 +
phosphate
 +
L-glutamine

Synonyms

AmGLN1, ATP glutamate-ammonia ligase, Chloroplast GS2, Clone lambda-GS28, Clone lambda-GS31, Clone lambda-GS8, Cytoplasmic GS3, Cytosolic GS1, gamma-glutamyl transferase, gamma-glutamyl:ammonia ligase, gamma-glutamylhydroxamate synthetase, Gln isozyme alpha, Gln isozyme beta, Gln isozyme gamma, Gln synthetase, GLN1,1, GLN1,2, GLN1,3, GLN1,4, GLN1-1, GLN1-2, Gln1;1, Gln1;2, Gln1;3, Gln1;4, Gln1;5, GLN2, GlnA, glnA-1, GlnA1, GlnA2, GlnA3, GlnA4, GlnN, GlnR, GluA, GLUL, Glutamate--ammonia ligase, glutamate-ammonia ligase, Glutamine synthetase, glutamine synthetase cytosolic isozyme 1, glutamine synthetase cytosolic isozyme 2, glutamine synthetase I, glutamine synthetase type II, glutamine synthetase type III, Glutamylhydroxamic synthetase, GS, GS type I, GS type-1, GS(1), GS-II, GS1, GS1 kinase, GS1-1, GS1-2, GS107, GS112, GS117, GS12, GS122, GS1a, GS1beta1, GS1gamma1, GS2, GS2a, GS3, GSI, GSII, GSIII, Isozyme delta, L-glutamate:ammonia ligase, L-glutamate:ammonia ligase (ADP-forming), L-Glutamine synthetase, LDBPK_060370, LdGS, MM_0964, MtGS, N47/N48, OsGLN1,1, OsGLN1,2, protein transacetylase, S2205/S2287, SSO0366, Synthetase, glutamine, TAase, type I glutamine synthetase, type III glutamine synthetase

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.1 Acid—ammonia (or amine) ligases (amide synthases)
                6.3.1.2 glutamine synthetase

Specific Activity

Specific Activity on EC 6.3.1.2 - glutamine synthetase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0002
-
crude cell extract
0.0149
-
after 75fold purification
0.018
-
glutamine synthetase activity in MG-63 cells
0.022
-
glutamine synthetase activity in MG-63 cells in presence of MG-132
0.023
-
glutamine synthetase activity in MG-63 cells in presence of dexamethasone and MG-132
0.042
-
glutamine synthetase activity in MG-63 cells in presence of vitamin D
0.067
-
glutamine synthetase activity in MG-63 cells in presence of dexamethasone
0.322
enzyme from promastigotes, pH 7.8, 37°C
0.7325
recombinant His-tagged enzyme, pH 7.8, 37°C
1.5
-
wild type strain
1.53
in the presence of 3 mM NaCl
11
pH not specified in the publication, temperature not specified in the publication
12.1
-
purified enzyme from strain SA1
126.1
-
purified enzyme from strain SA0
152
-
gamma-glutamylhydroxamate assay
153
-
gamma-glutamyl transferase assay
2.7
-
mutant enzyme S186F
221.3
-
gamma-glutamyl transferase assay
23
pH 6.5, 37°C, presence of Mn2+
26.8
80°C, purified enzyme
33.5
-
chloroplastic isoform GS2
33.9
-
gamma-glutamylhydroxamate assay
3300
purified recombinant enzyme, pH 7.9, 37°C
35.1
-
cytoplasmic isoform GS1
4.4
-
biosynthetic assay
41.6
-
gamma-glutamylhydroxamate assay
62
-
gamma-glutamylhydroxamate assay
73.2
-
gamma-glutamyl transferase assay
88.08
-
gamma-glutamyl transferase assay
99.6
-
gamma-glutamyl transferase assay
additional information