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6.3.1.2: glutamine synthetase

This is an abbreviated version!
For detailed information about glutamine synthetase, go to the full flat file.

Word Map on EC 6.3.1.2

Reaction

ATP
+
L-glutamate
 +
NH3
=
ADP
 +
phosphate
 +
L-glutamine

Synonyms

AmGLN1, ATP glutamate-ammonia ligase, Chloroplast GS2, Clone lambda-GS28, Clone lambda-GS31, Clone lambda-GS8, Cytoplasmic GS3, Cytosolic GS1, gamma-glutamyl transferase, gamma-glutamyl:ammonia ligase, gamma-glutamylhydroxamate synthetase, Gln isozyme alpha, Gln isozyme beta, Gln isozyme gamma, Gln synthetase, GLN1,1, GLN1,2, GLN1,3, GLN1,4, GLN1-1, GLN1-2, Gln1;1, Gln1;2, Gln1;3, Gln1;4, Gln1;5, GLN2, GlnA, glnA-1, GlnA1, GlnA2, GlnA3, GlnA4, GlnN, GlnR, GluA, GLUL, Glutamate--ammonia ligase, glutamate-ammonia ligase, Glutamine synthetase, glutamine synthetase cytosolic isozyme 1, glutamine synthetase cytosolic isozyme 2, glutamine synthetase I, glutamine synthetase type II, glutamine synthetase type III, Glutamylhydroxamic synthetase, GS, GS type I, GS type-1, GS(1), GS-II, GS1, GS1 kinase, GS1-1, GS1-2, GS107, GS112, GS117, GS12, GS122, GS1a, GS1beta1, GS1gamma1, GS2, GS2a, GS3, GSI, GSII, GSIII, Isozyme delta, L-glutamate:ammonia ligase, L-glutamate:ammonia ligase (ADP-forming), L-Glutamine synthetase, LDBPK_060370, LdGS, MM_0964, MtGS, N47/N48, OsGLN1,1, OsGLN1,2, protein transacetylase, S2205/S2287, SSO0366, Synthetase, glutamine, TAase, type I glutamine synthetase, type III glutamine synthetase

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.1 Acid—ammonia (or amine) ligases (amide synthases)
                6.3.1.2 glutamine synthetase

Purification

Purification on EC 6.3.1.2 - glutamine synthetase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography
-
cytoplasmic isoform GS1 and chloroplastic isoform GS2
-
DEAE-Sepharose Cl-6B chromatography
-
enzyme form GSIII
-
from both strains
-
from frozen and non-frozen tissue
-
from leaves, separation of GS1 and GS2 proteins by ion exchange chromatography
from nodule extract
-
HPLC immunoaffinity chromatography
-
isoenzymes GScs from seed cytosol and isoenzyme GScl from leaf cytosol
-
isoform GSI and GSII
-
Makroprep HighQ chromatography
-
Ni-NTA affinity chromatography and P10 Sephadex column chromatography
-
partial
partial, isoenzyme GS1
-
partial, isoenzyme GS2
-
purification methods
-
purification of unadenylated form
-
rapid and scalable two-step protocol for expression and purification of glutamine synthetase in an auxotrophic Escherichia coli strain utilizing differential precipitation by divalent cations followed by affinity chromatography to produce suitable quantities of homogenous material for structural characterization
recombinant enzyme
recombinant enzyme from Escherichia coli by nickel affinity chromatography, native enzyme 24.2fold to homogeneity by hydrophobic interaction chromatography followed by two different steps of anion exchange chromatography
-
recombinant enzyme glutamine synthetase type III
recombinant enzymes
-
recombinant enzymes and enzyme from host strain
recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, tag cleavage by 3C protease, and
recombinant His-tagged enzyme from Escherichia coli strain BL21 Codon PLUS-RIL by nickel affinity chromatography and gel filtration
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
recombinant His-tagged enzyme from Escherichia coli strain glnA mutant JW3841-1 by nickel affinity chromatography and dialysis
recombinant HIs-tagged isozymes GSI, GSIII-1, and GSIII-2 from Escherichia coli strain BL21 CodonPlus (DE3) RIL by nickel affinity chromatograaphy
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography, urea treatment, tag cleavage by thrombin, again nickel affinity chromatography to remove the tag, and dialysis
recombinant His6-tagged isozyme GS2a from Escherichia coli strain BL21(DE3) Codon Plus by m´nickel affinity chromatography
recombinant isoenzyme GLN1,1
recombinant isoenzyme GLN1,2
recombinant isoenzyme GLN1,3
recombinant isoenzyme GLN1,4
recombinant isozyme MtGS1a from Escherichia coli
recombinant mutant and wild-type enzyme
-
recombinant protein
recombinant proteins using His-tag
-
refolded recombinant nearly pure enzyme by anion exchange chromatography
Sephacryl 300 HR gel filtration
Sephacryl S-300 HR gel filtration
Sepharose-4B column chromatography, DEAE-cellulose column chromatography, and Sephacryl S-300 gel filtration
-
two enzyme forms EI and EII
-