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6.3.1.2: glutamine synthetase

This is an abbreviated version!
For detailed information about glutamine synthetase, go to the full flat file.

Word Map on EC 6.3.1.2

Reaction

ATP
+
L-glutamate
 +
NH3
=
ADP
 +
phosphate
 +
L-glutamine

Synonyms

AmGLN1, ATP glutamate-ammonia ligase, Chloroplast GS2, Clone lambda-GS28, Clone lambda-GS31, Clone lambda-GS8, Cytoplasmic GS3, Cytosolic GS1, gamma-glutamyl transferase, gamma-glutamyl:ammonia ligase, gamma-glutamylhydroxamate synthetase, Gln isozyme alpha, Gln isozyme beta, Gln isozyme gamma, Gln synthetase, GLN1,1, GLN1,2, GLN1,3, GLN1,4, GLN1-1, GLN1-2, Gln1;1, Gln1;2, Gln1;3, Gln1;4, Gln1;5, GLN2, GlnA, glnA-1, GlnA1, GlnA2, GlnA3, GlnA4, GlnN, GlnR, GluA, GLUL, Glutamate--ammonia ligase, glutamate-ammonia ligase, Glutamine synthetase, glutamine synthetase cytosolic isozyme 1, glutamine synthetase cytosolic isozyme 2, glutamine synthetase I, glutamine synthetase type II, glutamine synthetase type III, Glutamylhydroxamic synthetase, GS, GS type I, GS type-1, GS(1), GS-II, GS1, GS1 kinase, GS1-1, GS1-2, GS107, GS112, GS117, GS12, GS122, GS1a, GS1beta1, GS1gamma1, GS2, GS2a, GS3, GSI, GSII, GSIII, Isozyme delta, L-glutamate:ammonia ligase, L-glutamate:ammonia ligase (ADP-forming), L-Glutamine synthetase, LDBPK_060370, LdGS, MM_0964, MtGS, N47/N48, OsGLN1,1, OsGLN1,2, protein transacetylase, S2205/S2287, SSO0366, Synthetase, glutamine, TAase, type I glutamine synthetase, type III glutamine synthetase

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.1 Acid—ammonia (or amine) ligases (amide synthases)
                6.3.1.2 glutamine synthetase

Storage Stability

Storage Stability on EC 6.3.1.2 - glutamine synthetase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, stable for several weeks
-
-20°C, 10 mM MgSO4, 4 mM ATP, 50 mM glutamate, 100 mM NaCl, 10% ethylene glycol, 20 mM Hepes buffer, pH 7.4, less than 5% loss of activity per week
-
-20°C, stable for several months
-
-20°C, stable for two months
-
-70°C, concentrated solution in 50% glycerol, 2 months, 4% loss of activity
-
0-4°C, stable for 10 months
-
25 mM imidazole/HCl buffer, pH 7.15, 5 mM MgCl, 10 mM sodium glutamate, 5 mM 2-mercaptoethanol and 1 mM EDTA, at -80°C completely stable for several months, at 4°C stable for several hours
-
4°C, 10% loss of activity of isoform GSII after 24 h, no loss of activity of isoform GSII
-
4°C, 2 M NaCl, 1 week, stable
4°C, in 0.25 M MOPS buffer with 1.5 M KCl, 50 h, 11% residual activity
-
4°C, in 0.25 M MOPS buffer with 1.5 M NaCl, 50 h, 60% residual activity
-
4°C, native enzyme stable for at least 3 months, 1.5fold decrease in activity with metal ion-free enzyme within one week
-
4°C, stable for several days
-
5°C, about 95% of the initial activity is maintained for 30 days in a state of ammonium sulfate precipitate (70% saturation)
-
at 4°C or in the frozen state, 200 mM potassium phosphate buffer, pH 7.2, 10 mM 2-ketoglutarate, 5 mM 2-mercaptoethanol, 1 mM EDTA, total loss of activity after 24 h, in presence of 25%-50% glycerol 80-90% retention of activity after 2 months
-