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Literature summary for 6.3.1.2 extracted from

  • Antonyuk, L.P.; Smirnova, V.E.; Kamnev, A.A.; Serebrennikova, O.B.; Vanoni, M.A.; Zanetti, G.; Kudelina, I.A.; Sokolov, O.I.; Ignatov, V.V.
    Influence of divalent cations on the catalytic properties and secondary structure of unadenylylated glutamine synthetase from Azospirillum brasilense (2001), BioMetals, 14, 13-22.
    View publication on PubMed
Search for more literature for 6.3.1.2

General Stability

General Stability Organism
native enzyme is more stable than metal ion-free enzyme Azospirillum brasilense

Inhibitors

Inhibitors Comment Organism Structure
glutamate product inhibition above 20 mM when enzyme is activated with Mg2+, no inhibition when activated with Mn2+ Azospirillum brasilense

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ required, strongly bound to enzyme, can not be removed by dialysis, removal of metal ions with EDTA results in conformational changes Azospirillum brasilense
Mg2+ required, most effective, strongly bound to enzyme, can not be removed by dialysis, removal of metal ions with EDTA results in conformational changes Azospirillum brasilense
Mn2+ slight activation, strongly bound to enzyme, can not be removed by dialysis, removal of metal ions with EDTA results in conformational changes Azospirillum brasilense

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + glutamate + NH4+ Azospirillum brasilense central enzyme of nitrogen metabolism, ammonia assimilation ADP + phosphate + L-glutamine
-
 ?
ATP + glutamate + NH4+ Azospirillum brasilense SP245 central enzyme of nitrogen metabolism, ammonia assimilation ADP + phosphate + L-glutamine
-
 ?

Organism

Organism UniProt Comment Textmining
Azospirillum brasilense
-
 nitrogen-fixing plant-associated bacterium
-
Azospirillum brasilense SP245
-
 nitrogen-fixing plant-associated bacterium
-

Purification (Commentary)

Purification (Comment) Organism
purification of unadenylated form Azospirillum brasilense

Storage Stability

Storage Stability Organism
4°C, native enzyme stable for at least 3 months, 1.5fold decrease in activity with metal ion-free enzyme within one week Azospirillum brasilense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + glutamate + NH4+
-
 Azospirillum brasilense ADP + phosphate + L-glutamine
-
 ?
ATP + glutamate + NH4+ central enzyme of nitrogen metabolism, ammonia assimilation Azospirillum brasilense ADP + phosphate + L-glutamine
-
 ?
ATP + glutamate + NH4+
-
 Azospirillum brasilense SP245 ADP + phosphate + L-glutamine
-
 ?
ATP + glutamate + NH4+ central enzyme of nitrogen metabolism, ammonia assimilation Azospirillum brasilense SP245 ADP + phosphate + L-glutamine
-
 ?

Subunits

Subunits Comment Organism
dodecamer composed of two hexameric rings Azospirillum brasilense

Cofactor

Cofactor Comment Organism Structure
additional information regulated by adenylation, unadenylated form shows highest activity Azospirillum brasilense