immunoassay that detects synthetase protein samples where the enzyme has been inactivated by repeated cycles of freezing and thawing, and in serum and cerebrospinal fluid where glutamine synthetase is undetectable by the enzyme activity assay
glutamine synthetase is a useful marker in tumour liver pathology, including hepatocellular adenomas and nodules in cirrhosis. Enzyme overexpression in focal nodular hyperplasia can be used as easy diagnostic tool in surgical pathology
inhibition of glutamine synthetase is one of the most promising strategies for the discovery of drugs against tuberculosis. The bone-targeting properties of the bisphosphonate compounds make them attractive agents for the treatment of bone tuberculosis
inhibition of glutamine synthetase is one of the most promising strategies for the discovery of drugs against tuberculosis. The bone-targeting properties of the bisphosphonate compounds make them attractive agents for the treatment of bone tuberculosis
glutamine synthetase is a target for disruption in the development of vaccines against both bovine and human tuberculosis. Glutamine synthetase is also a potential target for tuberculosis chemotherapy
treatment with methionine sulfoximine of transgenic mice that overexpresses the mutant human superoxide dismutase SOD1G93A gene, an animalmodel for the primary inherited form of the human neurodegenerative disease amyotrophic lateral sclerosis. This treatment in vivo reduces glutamine synthetase activity measured in vitro by 85% and reduces brain levels of glutamine by 60% and of glutamate by 30% in both the motor cortex and the anterior striatum, while also affecting levels of GABA and glutathione. Methionine sulfoxime treatment significantly extends the lifespan of these mice by 8%
expression of glutathione synthetase is observed in 70% of hepatocellular carcinoma patients, 46.7% of chronic hepatitis B stage 4 patients and 38% of chronic hepatitis B stage 1-3 patients. There is significant difference between hepatocellular carcinoma samples and non-tumor tissues. Serum glutathione synthetase levels are increased in hepatocellular carcinoma and chronic hepatitis B stage 1-4 patients. There are significant differences among all samples, except chronic hepatitis B stage 1-3 versus chronic hepatitis B stage 4. Hepatocellular carcinoma, chronic hepatitis B stage 1-4 and AFP are significantly associated with serum glutathione synthetase levels. In hepatocellular carcinoma group, TNM and Child-Pugh are significantly associated with glutathione synthetase levels. Expression of glutathione synthetase is increased in hepatocellular carcinoma, liver cirrhosis, and chronic hepatitis B
glutamine synthetase is a target for disruption in the development of vaccines against both bovine and human tuberculosis. Glutamine synthetase is also a potential target for tuberculosis chemotherapy
since glutamine synthetase is the first metabolic enzyme involved in Trypanosoma cruzi evasion from the parasitophorous vacuole it is a potential target for designing anti-Trypanosoma cruzi drugs
since glutamine synthetase is the first metabolic enzyme involved in Trypanosoma cruzi evasion from the parasitophorous vacuole it is a potential target for designing anti-Trypanosoma cruzi drugs
high efficiency L-Gln production by coupling genetically engineered Bacillus subtilis glutamine synthetase with yeast alcoholic fermentation system using small ubiquitin-related modifier fusion technology SUMO and 0.1% lactose as inducer. Fusion protein is expressed in totally soluble form in Escherichia coli and can be purified to 90% purity by nickel nitrilo-triacetic acid resin chromatography with a yield of 625 mg per liter fermentation culture. About 121 mg recombinant GS is obtained from 1 l fermentation culture with 96% purity and 23 U/mg activity
rapid and scalable two-step protocol for expression and purification of glutamine synthetase in an auxotrophic Escherichia coli strain utilizing differential precipitation by divalent cations followed by affinity chromatography to produce suitable quantities of homogenous material for structural characterization