Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methionine sulfoximine | 2.5 mM causes up to 60% inhibition of the transferase GS activity and in the presence of 6 mM, the transferase GS activity is around 20% of the controls | Haloferax mediterranei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
ATP | at 45°C | Haloferax mediterranei | |
4.9 | - |
L-Glu | at 45°C | Haloferax mediterranei | |
10.5 | - |
hydroxylamine | at 45°C | Haloferax mediterranei | |
25 | - |
L-Gln | at 45°C | Haloferax mediterranei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulates activity at 1 mM | Haloferax mediterranei | |
Co2+ | stimulates activity at 1 mM | Haloferax mediterranei | |
Cu2+ | stimulates activity at 1 mM | Haloferax mediterranei | |
Fe2+ | stimulates activity at 1 mM | Haloferax mediterranei | |
K+ | 0.25 mM as optimal concentration for highest activity | Haloferax mediterranei | |
Mg2+ | stimulates activity at 1 mM | Haloferax mediterranei | |
Mn2+ | most effective cation for stimulation of activity at 1 mM | Haloferax mediterranei | |
Na+ | 0.5 mM as optimal concentration for highest activity | Haloferax mediterranei | |
Ni2+ | stimulates activity at 1 mM | Haloferax mediterranei | |
Zn2+ | stimulates activity at 1 mM | Haloferax mediterranei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51700 | - |
8 * 51700, gel filtration | Haloferax mediterranei |
445000 | - |
gel filtration | Haloferax mediterranei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax mediterranei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Sepharose-4B column chromatography, DEAE-cellulose column chromatography, and Sephacryl S-300 gel filtration | Haloferax mediterranei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0002 | - |
crude cell extract | Haloferax mediterranei |
0.0149 | - |
after 75fold purification | Haloferax mediterranei |
Storage Stability | Organism |
---|---|
4°C, in 0.25 M MOPS buffer with 1.5 M KCl, 50 h, 11% residual activity | Haloferax mediterranei |
4°C, in 0.25 M MOPS buffer with 1.5 M NaCl, 50 h, 60% residual activity | Haloferax mediterranei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + NH4+ | - |
Haloferax mediterranei | ADP + phosphate + L-Gln | - |
? | |
L-Gln + hydroxylamine + ADP | - |
Haloferax mediterranei | gamma-glutamylhydroxamate + NH4+ + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 51700, gel filtration | Haloferax mediterranei |
Synonyms | Comment | Organism |
---|---|---|
Glutamine synthetase | - |
Haloferax mediterranei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Haloferax mediterranei |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | - |
Haloferax mediterranei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Haloferax mediterranei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Haloferax mediterranei |