General Stability | Organism |
---|---|
the presence of Mn2+ or Mg2+ drastically affects the thermostability of transferase activity and biosynthetic activity | Phormidium laminosum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | biosynthetic activity | Phormidium laminosum | |
Ala | - |
Phormidium laminosum | |
AMP | biosynthetic activity | Phormidium laminosum | |
Asp | - |
Phormidium laminosum | |
Gly | - |
Phormidium laminosum | |
L-Arg | biosynthetic activity | Phormidium laminosum | |
L-Glu | inhibition of transferase activity, no inhibition of biosynthetic activity | Phormidium laminosum | |
L-Lys | - |
Phormidium laminosum | |
L-Met | - |
Phormidium laminosum | |
L-methionine sulfoximine | - |
Phormidium laminosum | |
L-Tyr | inhibits transferase activity, no inhibition of biosynthetic activity | Phormidium laminosum | |
p-hydroxymercuribenzoate | biosynthetic activity | Phormidium laminosum | |
Ser | - |
Phormidium laminosum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | partially effective as activator of biosynthetic activity and transferase activity, half-maximal activation of biosynthetic reaction at 6.3 mM | Phormidium laminosum | |
Cu2+ | partially effective as activator of transferase activity | Phormidium laminosum | |
Mg2+ | essential activator for biosynthetic activity, half-maximal activation at 18 mM. Partially effective as activator of transferase activity | Phormidium laminosum | |
Mn2+ | required for transferase activity | Phormidium laminosum | |
Zn2+ | partially effective as activator of biosynthetic activity, half-maximal activation at 6.3 mM | Phormidium laminosum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
602000 | - |
gel filtration | Phormidium laminosum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phormidium laminosum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Phormidium laminosum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine | ordered three-reactant system with the binding order: NH4+, L-Glu and ATP | Phormidium laminosum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Phormidium laminosum |
4.4 | - |
biosynthetic assay | Phormidium laminosum |
152 | - |
gamma-glutamylhydroxamate assay | Phormidium laminosum |
Storage Stability | Organism |
---|---|
-20°C, stable for several months | Phormidium laminosum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + NH4+ | - |
Phormidium laminosum | ADP + phosphate + L-Gln | - |
? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | SDS-PAGE of 2-mercaptoethanol-treated enzyme | Phormidium laminosum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
transferase activity | Phormidium laminosum |
55 | - |
biosynthetic activity | Phormidium laminosum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Phormidium laminosum |
7.5 | - |
transferase activity | Phormidium laminosum |