6.3.1.2: glutamine synthetase

This is an abbreviated version!
For detailed information about glutamine synthetase, go to the full flat file.

Reaction

Synonyms

AmGLN1, ATP glutamate-ammonia ligase, Chloroplast GS2, Clone lambda-GS28, Clone lambda-GS31, Clone lambda-GS8, Cytoplasmic GS3, Cytosolic GS1, gamma-glutamyl transferase, gamma-glutamyl:ammonia ligase, gamma-glutamylhydroxamate synthetase, Gln isozyme alpha, Gln isozyme beta, Gln isozyme gamma, Gln synthetase, GLN1,1, GLN1,2, GLN1,3, GLN1,4, GLN1-1, GLN1-2, Gln1;1, Gln1;2, Gln1;3, Gln1;4, Gln1;5, GLN2, GlnA, glnA-1, GlnA1, GlnA2, GlnA3, GlnA4, GlnN, GlnR, GluA, GLUL, Glutamate--ammonia ligase, glutamate-ammonia ligase, Glutamine synthetase, glutamine synthetase cytosolic isozyme 1, glutamine synthetase cytosolic isozyme 2, glutamine synthetase I, glutamine synthetase type II, glutamine synthetase type III, Glutamylhydroxamic synthetase, GS, GS type I, GS type-1, GS(1), GS-II, GS1, GS1 kinase, GS1-1, GS1-2, GS107, GS112, GS117, GS12, GS122, GS1a, GS1beta1, GS1gamma1, GS2, GS2a, GS3, GSI, GSII, GSIII, Isozyme delta, L-glutamate:ammonia ligase, L-glutamate:ammonia ligase (ADP-forming), L-Glutamine synthetase, LDBPK_060370, LdGS, MM_0964, MtGS, N47/N48, OsGLN1,1, OsGLN1,2, protein transacetylase, S2205/S2287, SSO0366, Synthetase, glutamine, TAase, type I glutamine synthetase, type III glutamine synthetase

ECTree

     6 Ligases

         6.3 Forming carbon-nitrogen bonds

             6.3.1 Acid—ammonia (or amine) ligases (amide synthases)

                6.3.1.2 glutamine synthetase

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Results	in table
66	Activating Compound
47140	AA Sequence
38	Application
1	CAS Registry Number
101	Cloned(Commentary)
98	Cofactor
15	Crystallization (Commentary)
4106	Disease/ Diagnostics
25	Expression
67	General Information
22	General Stability
139	IC50 Value
641	Inhibitors
20	kcat/KM [mM/s]
126	Ki Value [mM]
321	KM Value [mM]
103	Localization
242	Metals/Ions
162	Molecular Weight [Da]
181	Natural Substrates/ Products (Substrates)
770	Organism
8	Oxidation Stability
14	Pathway
630	PDB ID
102	pH Optimum
16	pH Range
9	pH Stability
23	pI Value
22	Posttranslational Modification
94	Protein Variants
100	Purification (Commentary)
30	Reaction
1	Reaction Type
387	Reference
3	Renatured (Commentary)
375	Source Tissue
60	Specific Activity [micromol/min/mg]
15	Storage Stability
566	Substrates and Products (Substrate)
161	Subunits
272	Synonyms
1	Systematic Name
48	Temperature Optimum [°C]
9	Temperature Range [°C]
45	Temperature Stability [°C]
46	Turnover Number [1/s]

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RENATURED/Commentary

ORGANISM

UNIPROT

LITERATURE

chaperonins, groEL and groES, enhance the renaturation of dodecameric enzyme with yields of active enzyme between 75% and 85% of the original activity

Escherichia coli

-

37534

mechanism of unfolding and refolding. More than 90% reconstitution of isoform E-I upon removal of 7-8 M guanidine-hydrochloride in the presence of 2-mercaptoethanol

[Bacillus] caldolyticus

-

37514

recombinant His-tagged nearly pure glutamine synthetase from inclusion bodies is solubilised at 37°C using 8 M urea as chaotrope in 20 mM Tris-HCl, pH 8.0, containing 2 mM EDTA and 50 mM DTT. The solubilised protein is then diluted in 20 mM Tris-HCl, pH 7.5, with 2 M NaCl and 5 mM DTT to a final protein concentration of 0.02 mg/ml and kept overnight at room temperature to enable proper folding

Haloferax mediterranei

B8ZJH0, F2RM17

727085