KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | 0.05 | NH4+ | isoforms GSI and GSII | Chlorella sorokiniana | |
0.3 | - |
ATP | isoform GSI | Chlorella sorokiniana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
8 * 43000, isoform GSII, SDS-PAGE | Chlorella sorokiniana |
47000 | - |
8 * 47000, isoform GSI, SDS-PAGE | Chlorella sorokiniana |
360000 | - |
isoform GSII, disc gel electrophoresis | Chlorella sorokiniana |
398000 | - |
isoform GSI, disc gel electrophoresis | Chlorella sorokiniana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorella sorokiniana | - |
oxygen-resistant mutant strain, isoforms GSI and GSII | - |
Purification (Comment) | Organism |
---|---|
isoform GSI and GSII | Chlorella sorokiniana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Chlorella sorokiniana |
18.5 | - |
- |
Chlorella sorokiniana |
Storage Stability | Organism |
---|---|
4°C, 10% loss of activity of isoform GSII after 24 h, no loss of activity of isoform GSII | Chlorella sorokiniana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + NH4+ | - |
Chlorella sorokiniana | ADP + phosphate + L-Gln | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 43000, isoform GSII, SDS-PAGE | Chlorella sorokiniana |
octamer | 8 * 47000, isoform GSI, SDS-PAGE | Chlorella sorokiniana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
isoform GSI and GSII are stable for 1 h | Chlorella sorokiniana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 8 | isoforms GSI and GSII | Chlorella sorokiniana |