Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Ovis aries | |
ADP | - |
Rattus norvegicus | |
ADP | - |
Sus scrofa | |
Ala | D-Ala | Rattus norvegicus | |
Ca2+ | above 2 mM CaCl2, in presence of 20 mM MgCl2 and 10 mM ATP | Rattus norvegicus | |
Carbamoyl phosphate | - |
Rattus norvegicus | |
D-Methionine sulfone | - |
Rattus norvegicus | |
D-Methionine sulfone | - |
Sus scrofa | |
diphosphate | - |
Rattus norvegicus | |
GDP | weak | Ovis aries | |
GDP | - |
Rattus norvegicus | |
GDP | - |
Sus scrofa | |
L-methionine sulfone | - |
Rattus norvegicus | |
L-methionine sulfone | - |
Sus scrofa | |
L-methionine sulfoximine | irreversible at high concentrations, competitive with L-Glu; S,R-sulfoximine and S-sulfoximine inhibit, R-sulfoximine is ineffective | Rattus norvegicus | |
L-methionine sulfoximine | - |
Sus scrofa | |
Mg2+ | inhibition at more than 20 mM excess of MgCl2 over ATP | Rattus norvegicus | |
Mn2+ | above 2 mM MnCl2, in presence of 20 mM MgCl2 and 10 mM ATP | Rattus norvegicus | |
phosphate | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Rattus norvegicus | |
Mg2+ | required | Sus scrofa | |
Mg2+ | optimal ratio of MgCl2 to ATP is 2:1 at 1 mM ATP, inhibition at more than 20 mM excess of MgCl2 over ATP | Rattus norvegicus | |
Mn2+ | optimal concentration is 2 mM, 25% of the maximal activity relative to Mg2+ activation | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ovis aries | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
brain | - |
Sus scrofa | - |
brain | - |
Ovis aries | - |
heart | - |
Sus scrofa | - |
kidney | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
muscle | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1 | - |
- |
Sus scrofa |
1.1 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + hydroxylamine | - |
Ovis aries | ADP + phosphate + gamma-glutamyl hydroxamate | - |
? | |
ATP + L-Glu + NH4+ | - |
Rattus norvegicus | ADP + phosphate + L-Gln | - |
? | |
ATP + L-Glu + NH4+ | - |
Sus scrofa | ADP + phosphate + L-Gln | - |
? | |
ATP + L-Glu + NH4+ | - |
Ovis aries | ADP + phosphate + L-Gln | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Rattus norvegicus |