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Literature summary for 6.3.1.2 extracted from

  • Hatanaka, M.; Tachiki, T.; Furukawa, R.; Tochikura, T.
    Purification and some properties of glutamine synthetases from Bifidobacteria (1987), Agric. Biol. Chem., 51, 425-433.
No PubMed abstract available
Search for more literature for 6.3.1.2

Inhibitors

Inhibitors Comment Organism Structure
adenosine more potent inhibitor in the reaction with Mg2+ than with Mn2+ Bifidobacterium bifidum
adenosine more potent inhibitor in the reaction with Mg2+ than with Mn2+ Bifidobacterium breve
adenosine more potent inhibitor in the reaction with Mg2+ than with Mn2+ Bifidobacterium pseudolongum
ADP more potent inhibition in the reaction with Mg2+ than with Mn2+ Bifidobacterium bifidum
ADP more potent inhibition in the reaction with Mg2+ than with Mn2+ Bifidobacterium breve
ADP more potent inhibition in the reaction with Mg2+ than with Mn2+ Bifidobacterium pseudolongum
Ala more inhibition by L-Ala and glycine in the mixture with Mn2+ than with Mg2+ Bifidobacterium bifidum
Ala more inhibition by L-Ala and glycine in the mixture with Mn2+ than with Mg2+ Bifidobacterium breve
Ala more inhibition by L-Ala and glycine in the mixture with Mn2+ than with Mg2+ Bifidobacterium pseudolongum
AMP more potent inhibitor in the reaction with Mg2+ than with Mn2+ Bifidobacterium bifidum
AMP more potent inhibitor in the reaction with Mg2+ than with Mn2+ Bifidobacterium breve
AMP more potent inhibitor in the reaction with Mg2+ than with Mn2+ Bifidobacterium pseudolongum
ATP ATP in excess of Mg2+ Bifidobacterium breve
ATP ATP in excess of Mg2+ Bifidobacterium pseudolongum
Ca2+
-
 Bifidobacterium bifidum
Ca2+
-
 Bifidobacterium pseudolongum
Cu2+
-
 Bifidobacterium bifidum
Cu2+
-
 Bifidobacterium pseudolongum
Fe2+
-
 Bifidobacterium bifidum
Fe2+
-
 Bifidobacterium pseudolongum
Gly more inhibition by L-Ala and Gly in the mixture with Mn2+ than with Mg2+ Bifidobacterium bifidum
Gly more inhibition by L-Ala and Gly in the mixture with Mn2+ than with Mg2+ Bifidobacterium breve
Gly more inhibition by L-Ala and Gly in the mixture with Mn2+ than with Mg2+ Bifidobacterium pseudolongum
Hg2+
-
 Bifidobacterium bifidum
Hg2+
-
 Bifidobacterium pseudolongum
Ni2+
-
 Bifidobacterium bifidum
Ni2+
-
 Bifidobacterium pseudolongum
Pb2+
-
 Bifidobacterium bifidum
Zn2+
-
 Bifidobacterium bifidum
Zn2+
-
 Bifidobacterium pseudolongum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.56
-
 NH4+ Mg2+-stimulated Bifidobacterium bifidum
0.59
-
 NH4+ Mg2+-stimulated Bifidobacterium breve
0.69
-
 NH4+ Mn2+-stimulated Bifidobacterium breve
0.69
-
 NH4+ NH4+, Mg2+-stimulated Bifidobacterium pseudolongum
0.71
-
 NH4+ Mn2+-stimulated Bifidobacterium pseudolongum
0.77
-
 NH4+ Mn2+-stimulated Bifidobacterium bifidum
1.1
-
 L-Glu Mg2+-stimulated Bifidobacterium bifidum
1.3
-
 L-Glu Mn2+-stimulated Bifidobacterium breve
1.5
-
 ATP Mg2+-stimulated Bifidobacterium pseudolongum
1.7
-
 ATP Mg2+-stimulated Bifidobacterium bifidum
2
-
 ATP Mg2+-stimulated or Mn2+-stimulated Bifidobacterium bifidum
2.1
-
 L-Glu Mn2+-stimulated Bifidobacterium pseudolongum
2.3
-
 ATP Mn2+-stimulated Bifidobacterium breve
2.5
-
 ATP Mn2+-stimulated Bifidobacterium pseudolongum
18
-
 L-Glu Mg2+-stimulated Bifidobacterium pseudolongum
21
-
 L-Glu Mg2+-stimulated Bifidobacterium breve
25
-
 L-Glu Mg2+-stimulated Bifidobacterium bifidum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ maximal activity at a ration of ATP to Co2+ of 1.0. The addition of 3 mM Co2+ to the biosynthetic reaction mixtures containing 4 mM Mg2+ results in a 3-7-fold increase in activity Bifidobacterium breve
Co2+ maximal activity at a ration of ATP to Co2+ of 1.0. The addition of 3 mM Co2+ to the biosynthetic reaction mixtures containing 4 mM Mg2+ results in a 3-7-fold increase in activity Bifidobacterium pseudolongum
Co2+ maximal activity when Co2+ is in excess of ATP Bifidobacterium bifidum
Co2+ divalent cation required, Mn2+, Co2+, or Mg2+. Mn2+ is most effective Bifidobacterium bifidum
Co2+ divalent cation required, Mn2+, Co2+, or Mg2+. Mn2+ is most effective Bifidobacterium breve
Co2+ divalent cation required, Mn2+, Co2+, or Mg2+. Mn2+ is most effective Bifidobacterium pseudolongum
Mg2+ divalent cation required, Mn2+, Co2+, or Mg2+. Mn2+ is most effective. Activity is maximal when Mg2+ is in excess of ATP Bifidobacterium bifidum
Mg2+ divalent cation required, Mn2+, Co2+, or Mg2+. Mn2+ is most effective. Activity is maximal when Mg2+ is in excess of ATP Bifidobacterium breve
Mg2+ divalent cation required, Mn2+, Co2+, or Mg2+. Mn2+ is most effective. Activity is maximal when Mg2+ is in excess of ATP Bifidobacterium pseudolongum
Mn2+ divalent cation required, Mn2+, Co2+, or Mg2+. Mn2+ is most effective. Activity is maximal at a ratio of ATP to Mn2+ of 1.0 Bifidobacterium bifidum
Mn2+ divalent cation required, Mn2+, Co2+, or Mg2+. Mn2+ is most effective. Activity is maximal at a ratio of ATP to Mn2+ of 1.0 Bifidobacterium breve
Mn2+ divalent cation required, Mn2+, Co2+, or Mg2+. Mn2+ is most effective. Activity is maximal at a ratio of ATP to Mn2+ of 1.0 Bifidobacterium pseudolongum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
500000
-
 gel filtration Bifidobacterium bifidum
500000
-
 gel filtration Bifidobacterium breve
500000
-
 gel filtration Bifidobacterium pseudolongum

Organism

Organism UniProt Comment Textmining
Bifidobacterium bifidum
-
 A
-
Bifidobacterium bifidum A
-
 A
-
Bifidobacterium breve
-
-
-
Bifidobacterium breve 203
-
-
-
Bifidobacterium pseudolongum
-
 A
-
Bifidobacterium pseudolongum A
-
 A
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bifidobacterium bifidum
-
 Bifidobacterium breve
-
 Bifidobacterium pseudolongum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
73.2
-
 gamma-glutamyl transferase assay Bifidobacterium breve
99.6
-
 gamma-glutamyl transferase assay Bifidobacterium pseudolongum
221.3
-
 gamma-glutamyl transferase assay Bifidobacterium bifidum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + ethylamine slightly effective Bifidobacterium bifidum ADP + phosphate + ?
-
 ?
ATP + L-Glu + ethylamine slightly effective Bifidobacterium breve ADP + phosphate + ?
-
 ?
ATP + L-Glu + ethylamine slightly effective Bifidobacterium pseudolongum ADP + phosphate + ?
-
 ?
ATP + L-Glu + ethylamine slightly effective Bifidobacterium bifidum A ADP + phosphate + ?
-
 ?
ATP + L-Glu + ethylamine slightly effective Bifidobacterium breve 203 ADP + phosphate + ?
-
 ?
ATP + L-Glu + ethylamine slightly effective Bifidobacterium pseudolongum A ADP + phosphate + ?
-
 ?
ATP + L-Glu + hydroxylamine 13% of the activity relative to NH4+ Bifidobacterium breve ADP + phosphate + gamma-glutamyl hydroxamate
-
 ?
ATP + L-Glu + hydroxylamine 59% of the activity relative to NH4+ Bifidobacterium bifidum ADP + phosphate + gamma-glutamyl hydroxamate
-
 ?
ATP + L-Glu + hydroxylamine 10% of the activity relative to NH4+ Bifidobacterium pseudolongum ADP + phosphate + gamma-glutamyl hydroxamate
-
 ?
ATP + L-Glu + hydroxylamine 59% of the activity relative to NH4+ Bifidobacterium bifidum A ADP + phosphate + gamma-glutamyl hydroxamate
-
 ?
ATP + L-Glu + hydroxylamine 13% of the activity relative to NH4+ Bifidobacterium breve 203 ADP + phosphate + gamma-glutamyl hydroxamate
-
 ?
ATP + L-Glu + hydroxylamine 10% of the activity relative to NH4+ Bifidobacterium pseudolongum A ADP + phosphate + gamma-glutamyl hydroxamate
-
 ?
ATP + L-Glu + methylamine slightly effective Bifidobacterium bifidum ADP + phosphate + ?
-
 ?
ATP + L-Glu + methylamine slightly effective Bifidobacterium breve ADP + phosphate + ?
-
 ?
ATP + L-Glu + methylamine slightly effective Bifidobacterium pseudolongum ADP + phosphate + ?
-
 ?
ATP + L-Glu + methylamine slightly effective Bifidobacterium bifidum A ADP + phosphate + ?
-
 ?
ATP + L-Glu + methylamine slightly effective Bifidobacterium breve 203 ADP + phosphate + ?
-
 ?
ATP + L-Glu + methylamine slightly effective Bifidobacterium pseudolongum A ADP + phosphate + ?
-
 ?
ATP + L-Glu + NH4+
-
 Bifidobacterium bifidum ADP + phosphate + L-Gln
-
 ?
ATP + L-Glu + NH4+
-
 Bifidobacterium breve ADP + phosphate + L-Gln
-
 ?
ATP + L-Glu + NH4+
-
 Bifidobacterium pseudolongum ADP + phosphate + L-Gln
-
 ?
Gln + hydroxylamine + ADP
-
 Bifidobacterium bifidum gamma-Glutamylhydroxamate + NH4+ + ?
-
 ?
Gln + hydroxylamine + ADP
-
 Bifidobacterium breve gamma-Glutamylhydroxamate + NH4+ + ?
-
 ?
Gln + hydroxylamine + ADP
-
 Bifidobacterium pseudolongum gamma-Glutamylhydroxamate + NH4+ + ?
-
 ?
Gln + hydroxylamine + ADP
-
 Bifidobacterium bifidum A gamma-Glutamylhydroxamate + NH4+ + ?
-
 ?
Gln + hydroxylamine + ADP
-
 Bifidobacterium breve 203 gamma-Glutamylhydroxamate + NH4+ + ?
-
 ?
Gln + hydroxylamine + ADP
-
 Bifidobacterium pseudolongum A gamma-Glutamylhydroxamate + NH4+ + ?
-
 ?
GTP + L-Glu + NH4+ no activity Bifidobacterium breve GDP + phosphate + L-Gln
-
 ?
GTP + L-Glu + NH4+ no activity Bifidobacterium pseudolongum GDP + phosphate + L-Gln
-
 ?
GTP + L-Glu + NH4+ 15% of the activity relative to ATP Bifidobacterium bifidum GDP + phosphate + L-Gln
-
 ?
GTP + L-Glu + NH4+ 15% of the activity relative to ATP Bifidobacterium bifidum A GDP + phosphate + L-Gln
-
 ?
GTP + L-Glu + NH4+ no activity Bifidobacterium breve 203 GDP + phosphate + L-Gln
-
 ?
GTP + L-Glu + NH4+ no activity Bifidobacterium pseudolongum A GDP + phosphate + L-Gln
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 pH 5.5, 10 min, 14% loss of activity Bifidobacterium bifidum
60
-
 pH 6.0, 10 min, 50% loss of activity Bifidobacterium breve
60
-
 pH 6.0, 10 min, 68% loss of activity Bifidobacterium pseudolongum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5 biosynthetic reaction, with 30 mM Mg2+ Bifidobacterium bifidum
6 6.5 biosynthetic reaction, with 30 mM Mg2+ Bifidobacterium breve
6 6.5 biosynthetic reaction, with 30 mM Mg2+ Bifidobacterium pseudolongum
6.5
-
 gamma-glutamylhydroxamate forming reaction Bifidobacterium bifidum
6.5
-
 gamma-glutamylhydroxamate forming reaction Bifidobacterium breve
6.5
-
 gamma-glutamylhydroxamate forming reaction Bifidobacterium pseudolongum

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 6 most stable in this range Bifidobacterium bifidum
5.5
-
 60°C, 10 min, 0.1 M acetate buffer, 14% loss of activity Bifidobacterium bifidum
6
-
 60°C, 10 min, 58% loss of activity Bifidobacterium pseudolongum
6
-
 60°C, 10 min, 0.1 M imidazole-HCl buffer, 50% loss of activity Bifidobacterium breve