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4.3.1.24: phenylalanine ammonia-lyase

This is an abbreviated version!
For detailed information about phenylalanine ammonia-lyase, go to the full flat file.

Word Map on EC 4.3.1.24

Reaction

L-phenylalanine
=
trans-cinnamate
 +
NH3

Synonyms

AtPAL 1, AtPAL 2, AtPAL 3, AtPAL 4, AtPAL-1, AtPAL-2, AtPAL-3, AtPAL-4, AtPAL2, AvPAL, DcPAL1, EC 4.3.1.5, EncP, L-phenylalanine ammonia-lyase, L-phenylalanine-ammonia lyase, LrPAL3, LsPAL1, More, PAL, PAL-CLEA, PAL1, PAL2, PAL3, PAL3a, PAL3b, PAL4, PAL5, PAL6, PALrs1, PcPAL1, Phe ammonia-lyase, phenylalanine ammonia lyase, phenylalanine ammonia-lyase, phenylalanine ammonia-lyase 1, phenylalanine ammonia-lyase 2, phenylalanine ammonia-lyase 3, phenylalanine ammonia-lyase 4, RgPAL, RxPAL, Sb04g026520, SsPAL1, TcPAL, ZmPAL2

ECTree

     4 Lyases
         4.3 Carbon-nitrogen lyases
             4.3.1 Ammonia-lyases
                4.3.1.24 phenylalanine ammonia-lyase

Molecular Weight

Molecular Weight on EC 4.3.1.24 - phenylalanine ammonia-lyase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
16180
-
calculated from cDNA
170000
-
gel filtration
225000
-
non-denaturing gradient PAGE
226000
Streptomyces verticillatus
-
-
255000
-
gel filtration
262000
PAL1 with a C-terminal His-tag, gel filtration
266000
-
gel filtration
275000 - 330000
-
-
280000
290000
gel filtration
300000
-
isoenzyme PAL 1, gel filtration
306000
-
-
309000
-
gel filtration
311000
-
gel filtration
316000
320000
323000
gel filtration
324000
-
gel filtration
330000
332000
-
gel filtration
336000
-
non-denaturing PAGE with 5-10% acrylamide
337500
-
non-denaturing PAGE with 4-20% acrylamide
340000
-
density gradient centrifugation
354000
-
non-denaturing PAGE
360000
-
non-denaturing PAGE
38000
-
4 * 38000, SDS-PAGE
380000
-
enzyme form PAL116, gel filtration
40000
-
4 * 40000, SDS-PAGE
55000
-
4 * 55000, SDS-PAGE
560000
-
enzyme form PAL68, gel filtration
60000
-
x * 60000, SDS-PAGE
64000
67020
x * 67020, calculated
70000
72000
-
x * 72000, SDS-PAGE
74000
75000
75500
-
calculated from cDNA
75711
4 * 75711, calculated
76000
76900
x * 76900, SDS-PAGE
76950
76970
76975
4 * 76975, calculated, 4 * 76000, SDS-PAGE
77000
77105
x * 77105, sequence calculation
77300
x * 77300, calculated
77450
x * 77450, PALrs1, sequence calcualtion
77600
x * 77600, SDS-PAGE
77828
-
4 * 77828, calöculation from nucleotide sequence
78000
-
x * 78000, SDS-PAGE
78139
x * 78139, calculated
78400
-
SDS-PAGE
78700
x * 78700, SDS-PAGE
79000
79100
83000
85000
-
alpha2,beta2, 2 * 75000 + 2 * 85000, SDS-PAGE
86000
90000
94000
x * 94000, SDS-PAGE of recombinant protein with GST-tag
additional information
wheat seedlings contain isozymes of 74 kDa, 83 kDa, and 103 kDa molecular weight