Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain X-33 and Escherichia coli Top10 cells | Bambusa oldhamii |
expression in Escherichia coli an Pichia pastoris | Bambusa oldhamii |
expression in Escherichia coli and Pichia pastoris | Bambusa oldhamii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
L-phenylalanine | recombinant enzyme expressed in Escherichia coli | Bambusa oldhamii | |
0.25 | - |
L-phenylalanine | recombinant enzyme expressed in Escherichia coli, at 37°C and pH 8.5 | Bambusa oldhamii | |
0.25 | - |
L-Phe | recombinant protein from Escherichia coli, pH 8.5, 37°C | Bambusa oldhamii | |
0.331 | - |
L-phenylalanine | recombinant enzyme expressed in Pichi pastoris | Bambusa oldhamii | |
0.331 | - |
L-phenylalanine | recombinant enzyme expressed in Pichia pastoris, at 37°C and pH 8.5 | Bambusa oldhamii | |
0.331 | - |
L-Phe | recombinant protein from Pichia pastoris, pH 8.5, 37°C | Bambusa oldhamii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76970 | - |
subunit, calculated from amino acid sequence | Bambusa oldhamii |
76970 | - |
4 * 76970, calculated, 4 * 80000, SDS-PAGE of recombinant protein with His-tag | Bambusa oldhamii |
80000 | - |
4 * 80000, SDS-PAGE | Bambusa oldhamii |
80000 | - |
4 * 76970, calculated, 4 * 80000, SDS-PAGE of recombinant protein with His-tag | Bambusa oldhamii |
330000 | - |
gel filtration | Bambusa oldhamii |
330000 | - |
SDS-PAGE | Bambusa oldhamii |
330000 | - |
PAGE and gel filtration | Bambusa oldhamii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bambusa oldhamii | - |
- |
- |
Bambusa oldhamii | C0LL35 | - |
- |
Bambusa oldhamii | C0LL35 | isoform Pal2 | - |
Purification (Comment) | Organism |
---|---|
HiTrap affinity chromatography | Bambusa oldhamii |
Ni-IDA column column chromatography | Bambusa oldhamii |
recombinant protein from Escherichia coli | Bambusa oldhamii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
shoot | - |
Bambusa oldhamii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.19 | - |
crude recombinant extract from Escherichia coli, at 37°C and pH 8.5 | Bambusa oldhamii |
0.41 | - |
crude recombinant extract from Pichia pastoris, at 37°C and pH 8.5 | Bambusa oldhamii |
3.76 | - |
recombinant enzyme from Pichia pastoris, after 9.1fold purification, at 37°C and pH 8.5 | Bambusa oldhamii |
3.76 | - |
recombinant protein from Pichia pastoris, pH 8.5, 37°C | Bambusa oldhamii |
3.77 | - |
recombinant enzyme expressed in Escherichia coli | Bambusa oldhamii |
3.77 | - |
recombinant protein from Escherichia coli, pH 8.5, 37°C | Bambusa oldhamii |
3.78 | - |
recombinant enzyme from Escherichia coli, after 19.4fold purification, at 37°C and pH 8.5 | Bambusa oldhamii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Phe | - |
Bambusa oldhamii | (E)-cinnamate + NH3 | - |
? | |
L-phenylalanine | - |
Bambusa oldhamii | (E)-cinnamate + NH3 | - |
? | |
L-phenylalanine | assay at pH 8.5, 37°C, reaction terminated by addition of HCl | Bambusa oldhamii | (E)-cinnamate + NH3 | - |
? | |
additional information | L-Tyr is a poor substrate | Bambusa oldhamii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 80000, SDS-PAGE | Bambusa oldhamii |
tetramer | 4 * 80000, SDS-PAGE | Bambusa oldhamii |
tetramer | 4 * 76970, calculated, 4 * 80000, SDS-PAGE of recombinant protein with His-tag | Bambusa oldhamii |
Synonyms | Comment | Organism |
---|---|---|
EC 4.3.1.5 | formerly | Bambusa oldhamii |
PAL | - |
Bambusa oldhamii |
PAL2 | - |
Bambusa oldhamii |
phenylalanine ammonia-lyase | - |
Bambusa oldhamii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
recombinant enzyme expressed in Escherichia coli | Bambusa oldhamii |
50 | 60 | - |
Bambusa oldhamii |
60 | - |
recombinant enzyme expressed in Pichi pastoris | Bambusa oldhamii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.12 | - |
L-phenylalanine | recombinant enzyme expressed in Escherichia coli | Bambusa oldhamii | |
10.12 | - |
L-phenylalanine | recombinant enzyme expressed in Escherichia coli, at 37°C and pH 8.5 | Bambusa oldhamii | |
10.12 | - |
L-Phe | recombinant protein from Escherichia coli, pH 8.5, 37°C | Bambusa oldhamii | |
16.04 | - |
L-phenylalanine | recombinant enzyme expressed in Pichi pastoris | Bambusa oldhamii | |
16.04 | - |
L-phenylalanine | recombinant enzyme expressed in Pichia pastoris, at 37°C and pH 8.5 | Bambusa oldhamii | |
16.04 | - |
L-Phe | recombinant protein from Pichia pastoris, pH 8.5, 37°C | Bambusa oldhamii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
recombinant enzyme expressed in Escherichia coli | Bambusa oldhamii |
8.5 | 9 | - |
Bambusa oldhamii |
9 | - |
recombinant enzyme expressed in Pichi pastoris | Bambusa oldhamii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | PAL2 sequence containes a conserved active site motif, the Ala-Ser-Gly triad, which can be converted into a 3,5-dihydro-5-methylidine-4H-imidazol-4-one prosthetic group | Bambusa oldhamii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.05 | - |
L-phenylalanine | recombinant enzyme expressed in Escherichia coli | Bambusa oldhamii | |
4.85 | - |
L-phenylalanine | recombinant enzyme expressed in Pichi pastoris | Bambusa oldhamii | |
40.5 | - |
L-Phe | recombinant protein from Escherichia coli, pH 8.5, 37°C | Bambusa oldhamii | |
48.5 | - |
L-Phe | recombinant protein from Pichia pastoris, pH 8.5, 37°C | Bambusa oldhamii |