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Literature summary for 4.3.1.24 extracted from
- Purification and properties of phenylalanine ammonia-lyase from leaf mustard (1997), Mol. Cells, 7, 715-720.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| caffeate | strong activation | Brassica juncea |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, 20% inhibition | Brassica juncea | |
| Cinnamic acid | trans-cinnamate does not inhibit | Brassica juncea | |
| Cu2+ | 1 mM, 33% inactivation | Brassica juncea | |
| Zn2+ | 1 mM, complete inhibition | Brassica juncea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
L-Phe | - |
Brassica juncea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
4 * 40000, SDS-PAGE | Brassica juncea |
| 170000 | - |
gel filtration | Brassica juncea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Brassica juncea | first enzyme in phenylpropanoid biosynthesis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica juncea | - |
var. integrifolia | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Brassica juncea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Phe | - |
Brassica juncea | (E)-cinnamate + NH3 | - |
? | |
| additional information | first enzyme in phenylpropanoid biosynthesis | Brassica juncea | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 40000, SDS-PAGE | Brassica juncea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Brassica juncea |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | 30°C: about 45% of maximal activity, 60°C: about 30% of maximal activity | Brassica juncea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Brassica juncea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | pH 7: about 40% of maximal activity, pH 10: about 85% of maximal activity | Brassica juncea |
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Release 2023.1 (January 2023)
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