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4.3.1.24: phenylalanine ammonia-lyase

This is an abbreviated version!
For detailed information about phenylalanine ammonia-lyase, go to the full flat file.

Word Map on EC 4.3.1.24

Reaction

L-phenylalanine
=
trans-cinnamate
+
NH3

Synonyms

AtPAL 1, AtPAL 2, AtPAL 3, AtPAL 4, AtPAL-1, AtPAL-2, AtPAL-3, AtPAL-4, AtPAL2, AvPAL, DcPAL1, EC 4.3.1.5, EncP, L-phenylalanine ammonia-lyase, L-phenylalanine-ammonia lyase, LrPAL3, LsPAL1, More, PAL, PAL-CLEA, PAL1, PAL2, PAL3, PAL3a, PAL3b, PAL4, PAL5, PAL6, PALrs1, PcPAL1, Phe ammonia-lyase, phenylalanine ammonia lyase, phenylalanine ammonia-lyase, phenylalanine ammonia-lyase 1, phenylalanine ammonia-lyase 2, phenylalanine ammonia-lyase 3, phenylalanine ammonia-lyase 4, RgPAL, RxPAL, Sb04g026520, SsPAL1, TcPAL, ZmPAL2

ECTree

     4 Lyases
         4.3 Carbon-nitrogen lyases
             4.3.1 Ammonia-lyases
                4.3.1.24 phenylalanine ammonia-lyase

General Stability

General Stability on EC 4.3.1.24 - phenylalanine ammonia-lyase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
encapsulated enzyme shows high operational stability of 7 reaction cycles
-
enzyme stabilization by 20% glycerol, 2-mercaptoethanol at 10 mM, 5 mM EDTA, and 2% ascorbic acid or 5 mM EDTA + 2% ascorbic acid
-
glycerol stabilizes the enzyme
roots retain 90% of their PAL activity after freeze-drying and about 40-60% activity after freeze-drying and a subsequent 15-week storage at 4°C
stability (thermal stability, pH-stability, stability of the enzyme against denaturants and storage stability) is improved by encapsulation
-
the plant cell walls provide protection of PAL during in vitro digestion
-