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4.3.1.24: phenylalanine ammonia-lyase

This is an abbreviated version!
For detailed information about phenylalanine ammonia-lyase, go to the full flat file.

Word Map on EC 4.3.1.24

Reaction

L-phenylalanine
=
trans-cinnamate
 +
NH3

Synonyms

AtPAL 1, AtPAL 2, AtPAL 3, AtPAL 4, AtPAL-1, AtPAL-2, AtPAL-3, AtPAL-4, AtPAL2, AvPAL, DcPAL1, EC 4.3.1.5, EncP, L-phenylalanine ammonia-lyase, L-phenylalanine-ammonia lyase, LrPAL3, LsPAL1, More, PAL, PAL-CLEA, PAL1, PAL2, PAL3, PAL3a, PAL3b, PAL4, PAL5, PAL6, PALrs1, PcPAL1, Phe ammonia-lyase, phenylalanine ammonia lyase, phenylalanine ammonia-lyase, phenylalanine ammonia-lyase 1, phenylalanine ammonia-lyase 2, phenylalanine ammonia-lyase 3, phenylalanine ammonia-lyase 4, RgPAL, RxPAL, Sb04g026520, SsPAL1, TcPAL, ZmPAL2

ECTree

     4 Lyases
         4.3 Carbon-nitrogen lyases
             4.3.1 Ammonia-lyases
                4.3.1.24 phenylalanine ammonia-lyase

Cloned

Cloned on EC 4.3.1.24 - phenylalanine ammonia-lyase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
C- or N-terminal His-tagged enzymes are expressed in Escherichia coli Rosetta 2 (DE3) cells
cloned into expression plasmid pHIS8. The recombinant EncP is overexpressed as an N-terminal octahistidyl-tagged fusion protein in Escherichia coli BL21
-
DNA and amino acid sequence determination and analysis, primers designed from GenBank sequence D26596, UniProt ID P45726, semiquantitative expression analysis by RT-PCR
DNA and amino acid sequence determination, analysis, and comparison of PAL isozymes, expression and phylogenetic analysis
expressed in Escherichia coli
expressed in Escherichia coli as a His-tagged fusion protein
expressed in Escherichia coli BL21 (DE3)
expressed in Nicotiana tabacum
expressed in Pichia pastoris strain X-33
expressed in Pichia pastoris strain X-33 and Escherichia coli Top10 cells
expression in Escherichia coli
expression in Escherichia coli an Pichia pastoris
expression in Escherichia coli and Pichia pastoris
expression in Escherichia coli BL21 (DE3)
expression in Escherichia coli or Saccharomyces cerevisiae
expression in Escherichia coli strain JM109
expression in Streptomyces lividans
-
fusion proteins with gluthatione S-transferase expressed in Escherichia coli
-
gene GbPAL, DNA and amino acid sequence determination and analysis, the gene is intron-less and belongs to a small multi-gene family, expression analysis and sequence comparison
gene pal1, genomic library screening, DNA and amino acid sequence determination and analysis, expression and phylogenetic analysis, recombinant expression in Escherichia coli strain XL1-Blue, identification of cis-acting regulatory DNA elements
gene pal1, transcription profile, expression in Escherichia coli strain M15
gene pal2, genomic library screening, DNA and amino acid sequence determination and analysis, expression and phylogenetic analysis, recombinant expression in Escherichia coli strain XL1-Blue, identification of cis-acting regulatory DNA elements
gene pal3a, genomic library screening, DNA and amino acid sequence determination and analysis, expression and phylogenetic analysis, recombinant expression in Escherichia coli strain XL1-Blue, identification of cis-acting regulatory DNA elements
gene pal3b, genomic library screening, DNA and amino acid sequence determination and analysis, expression and phylogenetic analysis, recombinant expression in Escherichia coli strain XL1-Blue, identification of cis-acting regulatory DNA elements
gene pal4, genomic library screening, DNA and amino acid sequence determination and analysis, expression and phylogenetic analysis, recombinant expression in Escherichia coli strain XL1-Blue, identification of cis-acting regulatory DNA elements
gene PALrs1, DNA and amino acid sequence determination and analysis, and analysis of egenomic structure, overview. Expression of PALrs1 under the 35S promoter with double-enhancer sequences from CaMV-W and TMV-W fragments in Rhodiola sachalinensis via Agrobacterium tumefaciens transfection method
overexpression in Coleus blumei
overexpression in Escherichia coli
PAL genes, multi-gene family with about 26 copies in the diploid genome, DNA and amino acid sequence determination and analysis, distribution of PAL gene sequences in the tomato genome, overview, very active silencing suggesting aggressive competition between PAL gene duplication and copy inactivation during PAL gene evolution, expression analysis of isozymes in plants, overview. Expression in Escherichia coli strain DH5alpha
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109
the expression of genes PAL1 and PAL2, but not PAL3, is upregulated in transgenic cells expressing recombinant plant oncogene rolB of Agrobacterium rhizogenes, expression analysis, overview
-
transient expression of a loss-of-function mutant DcPAL3 promoter-reporter construct in protoplasts prepared from carrot suspension-cultured cells, both the GCC-box homolog and the box-L are required for overall DcPAL3 expression. Possible different mechanism in the normal and variant cultured cell lines, respectively, in terms DcPAL3 promoter regulation