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3.6.1.9: nucleotide diphosphatase

This is an abbreviated version!
For detailed information about nucleotide diphosphatase, go to the full flat file.

Word Map on EC 3.6.1.9

Reaction

a nucleoside triphosphate
+
H2O
=
A nucleotide
 +
diphosphate

Synonyms

ADPglucose pyrophosphatase/phosphodiesterase, AGPPase, AGPPase1, AGPPase2, alkaline sphingomyelinase nucleotide pyrophosphatase phosphodiesterase 7, Apyrase, ASMTL-Maf, BSU28050, CBS-PPase, CBS-pyrophosphatase, CD203c, CD39, diphosphopyridine nucleotide pyrophosphatase, dITP pyrophosphatase, dITPase, E-NPP, E-NPP1, E-NPP3, E-NTPDase, E-type ATPDase, EC 3.6.1.19, ecto-ATP-diphosphohydrolase, ecto-ATPDase, ecto-nucleoside triphosphate diphosphohydrolase, ecto-nucleoside triphosphate diphosphohydrolase 2, ecto-nucleotide phosphodiesterase/pyrophosphatase 6 eNPP6, ecto-nucleotide pyrophosphatase, ecto-nucleotide pyrophosphatase/phosphodiesterase, ecto-nucleotide pyrophosphatase/phosphodiesterase I-3, ecto-nucleotide pyrophosphatase/phosphodiesterase1, ecto-nucleotide pyrophosphatase/phosphodiesterase3, ecto-nucleotide pyrophosphatase/phosphodiesterases, ectonucleotide pyrophosphatase/phosphodiesterase, ectonucleotide pyrophosphatase/phosphodiesterase 1, ectonucleotide pyrophosphatase/phosphodiesterase family member 3, ENPP1, ENPP3, ENTPD1, Entpd6, family II pyrophosphatase, h-NPP-1, h-NPP-3, h-NPP1, h-NPP3, HAM1, inosine triphosphatase, inosine triphosphate pyrophosphatase, inosine triphosphate pyrophosphohydrolase, ITPA, ITPase, Maf protein, Maf-like protein YhdE, MazG, MazG protein, MazG-related protein, More, MutT, NPP, NPP 1, NPP 2, NPP 3, NPP-5, NPP1, NPP2, NPP3, NPP6, NPP7, NPPase, NPPr, NTPDase, nucleoside triphosphate diphosphatase, nucleoside triphosphate diphosphohydrolase, nucleoside triphosphate pyrophosphatase, nucleoside triphosphate pyrophosphohydrolase, nucleoside-triphosphate pyrophosphatase, nucleotide pyrophosphastases/phosphodiesterases1, nucleotide pyrophosphastases/phosphodiesterases3, nucleotide pyrophosphatase, nucleotide pyrophosphatase-1, nucleotide pyrophosphatase-3, nucleotide pyrophosphatase-5, nucleotide pyrophosphatase/phosphodiesterase, nucleotide pyrophosphatase/phosphodiesterase 1, nucleotide pyrophosphatase/phosphodiesterase NPP1, nucleotide pyrophosphatase/phosphodiesterase-1, nucleotide pyrophosphatase/phosphodiesterase-I, nucleotide pyrophosphatase/phosphodiesterases, nucleotide pyrophosphohydrolase, nucleotide-sugar pyrophosphatase, nucleotide-sugar pyrophosphatase/phosphodiesterase, ONPP, PC-1, plasma cell membrane glycoprotein-1, PPase, pyrophosphatase, nucleoside triphosphate, RdgB, RS21-C6, SAGPPase1, SAGPPase2, Tm-MazG, TM0159, TM0913, YhdE, YJR069C, YOR111W, ZmNPP1, ZmNPP2, ZmNPP3

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.1 In phosphorus-containing anhydrides
                3.6.1.9 nucleotide diphosphatase

Purification

Purification on EC 3.6.1.9 - nucleotide diphosphatase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
AGPPase1
-
AGPPase2
-
blue-Sepharose column chromatography, oligo-dT cellulose column chromatography, and Sephacryl S-300 gel filtration
-
cellulose chromatography
-
chromatography techniques
-
hydroxylapatite column chromatpgraphy, DEAE Sepharose column chromatography, and Superdex 200 gel filtration
-
native enzyme from wheat germs, recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography
near homogeneity
-
Ni-charged column chromatography and gel filtration
partial
recombinant wild-type and mutant enzymes from Pichia pastoris culture medium by concanavalin A affinity chromatography, elution with methyl-alpha-D-glucopyranoside, anion exchange chromatography, dialysis, and another step of anion exchange chromatography followed by gel filtration
soluble native enzyme 618fold from fruits to homogeneity by ammonium sulfate fractionation, heat treatment at 75°C, anion exchange chromatography, and gel filtration
-
to homogemeity, chromatography steps, trifunctional enzyme, displaying phosphodiesterase-1/diphosphatase- and protein threonin kinase activity, that also phosphorylates histone IIa
-
to homogeneity, affinity chromatography
to homogeneity, chromatography rechniques
-
to homogeneity, chromatography techniques
to homogeneity, enzymatically active monomer, dimer and tetramer
-
to homogeneity, immunoaffinity chromatography
-