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3.5.1.4: amidase

This is an abbreviated version!
For detailed information about amidase, go to the full flat file.

Word Map on EC 3.5.1.4

Reaction

a monocarboxylic acid amide
+
H2O
=
a monocarboxylate
 +
NH3

Synonyms

4-guanidinobutyramide hydrolase, A-amidase, acetamidase, acid transferase, acylamidase, acylase, AMI1, amidase, amidase 1, amide hydrolase, amide transferase, amidohydrolase, AmiE, Ana, anandamide hydrolase, AtFAAH, Azl13, C-amidase, deaminase, ester transferase, FAAH, FAAH-1, FAAH-2, fatty acid amide hydrolase, fatty acid amide hydrolase 1, fatty acylamidase, half-amidase, indole-3-acetamide amidohydrolase, K1, mHG, Mhpg, More, murein peptide amidase A, mycothiol-S-conjugate amidase, N-acetylaminohydrolase, N-acylethanolamine acid amidase, N-acylethanolamine-hydrolyzing acid amidase, N-acylethanolaminehydrolyzing acid amidase, NAAA, NAE-hydrolyzing acid amidase, PamH, PYCH_10400, R-stereospecific amidase, RhAmidase, signature amidase, SsAH, SSAM, SSO2122, tissue kallikrein, Wide spectrum amidase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.4 amidase

Turnover Number

Turnover Number on EC 3.5.1.4 - amidase

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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.9
2-Aminobenzamide
pH 7.5, 70°C
1.3
2-hydroxybenzamide
pH 7.5, 70°C
129
2-nitroacetanilide
-
-
0.3 - 0.8
2-toluamide
1.8
3-indolacetamide
pH 7.5, 70°C
12.82
3-phenyl-propionamide
-
pH 5.0, 70°C, wild-type enzyme
7.1 - 18
3-phenylpropionamide
0.8
4-Aminobenzamide
pH 7.5, 70°C
55.6
4-Guanidinobutyramide
pH 8.0, 35°C, recombinant enzyme
1.1 - 2.1
4-hydroxybenzamide
0.8 - 2.5
4-nitrobenzamide
144
4-nitrophenylacetate
-
-
7.3 - 14.2
4-toluamide
-
0.143 - 5051
acetamide
134
acetanilide
-
-
47.4
acetate
-
-
10 - 20
acetohydrazide
14
Acetohydroxamate
-
hydrolysis
0.0715 - 333.3
Acrylamide
0.00217 - 1246
Benzamide
2.04 - 34.3
butyramide
769 - 906
cinnamamide
16.3
cyclohexanamide
pH 7.5, 70°C
11.9
cyclomaltohexaose
-
pH 5.0, 70°C, wild-type enzyme
0.0372
glutaramic acid
Blastobacter sp.
-
-
5.3 - 721.7
hexanamide
1.2 - 2.2
indol-3-acetamide
5.68 - 175
Isobutyramide
1.6
Isonicotinamide
pH 7.5, 70°C
887
ketoprofen amide
-
-
7216
L-glutamine
pH 7.6, 60°C
3.1
L-prolinamide
pH 7.5, 70°C
10.7
lactamide
pH 7.5, 70°C
3 - 5.75
metacrylamide
2.9
methacrylamide
pH 7.5, 70°C
1.08 - 890
N-Methylacetamide
0.3
N-methylpropionamide
-
hydrolysis
1.6 - 6.4
nicotinamide
20.5 - 28.6
Pentanamide
147
phenylacetate
-
-
1.8
picolinamide
pH 7.5, 70°C
3.2 - 465
Propionamide
9.5
propionohydrazide
-
transferase reaction
22
propionohydroxamate
-
hydrolysis
0.096
succinamic acid
Blastobacter sp.
-
-