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3.5.1.4: amidase

This is an abbreviated version!
For detailed information about amidase, go to the full flat file.

Word Map on EC 3.5.1.4

Reaction

a monocarboxylic acid amide
+
H2O
=
a monocarboxylate
 +
NH3

Synonyms

4-guanidinobutyramide hydrolase, A-amidase, acetamidase, acid transferase, acylamidase, acylase, AMI1, amidase, amidase 1, amide hydrolase, amide transferase, amidohydrolase, AmiE, Ana, anandamide hydrolase, AtFAAH, Azl13, C-amidase, deaminase, ester transferase, FAAH, FAAH-1, FAAH-2, fatty acid amide hydrolase, fatty acid amide hydrolase 1, fatty acylamidase, half-amidase, indole-3-acetamide amidohydrolase, K1, mHG, Mhpg, More, murein peptide amidase A, mycothiol-S-conjugate amidase, N-acetylaminohydrolase, N-acylethanolamine acid amidase, N-acylethanolamine-hydrolyzing acid amidase, N-acylethanolaminehydrolyzing acid amidase, NAAA, NAE-hydrolyzing acid amidase, PamH, PYCH_10400, R-stereospecific amidase, RhAmidase, signature amidase, SsAH, SSAM, SSO2122, tissue kallikrein, Wide spectrum amidase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.4 amidase

KCat KM Value

KCat KM Value on EC 3.5.1.4 - amidase

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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.3
2-Aminobenzamide
pH 7.5, 70°C
3.3
2-hydroxybenzamide
pH 7.5, 70°C
0.75 - 4
2-toluamide
2
3-indolacetamide
pH 7.5, 70°C
5.44
3-phenyl-propionamide
-
pH 5.0, 70°C, wild-type enzyme
1.8 - 7.2
3-phenylpropionamide
0.27
4-Aminobenzamide
pH 7.5, 70°C
24.17
4-Guanidinobutyramide
pH 8.0, 35°C, recombinant enzyme
0.73 - 21
4-hydroxybenzamide
4 - 8.33
4-nitrobenzamide
0.44 - 606.3
acetamide
0.087 - 30.69
Acrylamide
0.034 - 153.5
Benzamide
2.45 - 8.25
butyramide
3.3
cyclohexanamide
pH 7.5, 70°C
24.3 - 61
cyclomaltohexaose
0.91 - 65.14
hexanamide
1.5 - 2.75
indol-3-acetamide
6.99 - 21.9
Isobutyramide
2
Isonicotinamide
pH 7.5, 70°C
453.2
L-glutamine
pH 7.6, 60°C
0.46
L-prolinamide
pH 7.5, 70°C
3.57
lactamide
pH 7.5, 70°C
6 - 16
metacrylamide
2.4
methacrylamide
pH 7.5, 70°C
0.042 - 5.5
nicotinamide
10.7 - 19
Pentanamide
0.72
picolinamide
pH 7.5, 70°C
0.64 - 9.94
Propionamide