Information on EC 3.5.1.4 - amidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.5.1.4
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RECOMMENDED NAME
GeneOntology No.
amidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a monocarboxylic acid amide + H2O = a monocarboxylate + NH3
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxylic acid amide hydrolysis
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hydrolysis of amide bond
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hydrolysis of carboxylic acid amide
transfer of acyl group
transfer of amide group
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
acrylonitrile degradation I
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indole-3-acetate biosynthesis II
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indole-3-acetate biosynthesis III (bacteria)
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indole-3-acetate biosynthesis IV (bacteria)
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L-arginine degradation X (arginine monooxygenase pathway)
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arginine metabolism
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degradation of aromatic, nitrogen containing compounds
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IAA biosynthesis
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Arginine and proline metabolism
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Phenylalanine metabolism
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Tryptophan metabolism
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Aminobenzoate degradation
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Styrene degradation
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
acylamide amidohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
9012-56-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
Aeribacillus pallidus BTP-5x MTCC 9225
isolated from thermal springs of Tatapani (Himachal Pradesh)
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Manually annotated by BRENDA team
strain FB188, or Bordetella sp.
SwissProt
Manually annotated by BRENDA team
Alcaligenes sp. MTCC 10674
isolated as a nitrile degrading bacterium from the soil
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
Blastobacter sp.
strain A17p-4
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
strain ZJB-07021
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Manually annotated by BRENDA team
strain R312
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Manually annotated by BRENDA team
strain AM10-C-1
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
strain C, hospital isolate
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Manually annotated by BRENDA team
an acrylamide-degrading bacterium isolated from the grounds of a palm estate in Serdang, Selangor; previously isolated from the grounds of a palm estate in Serdang, Selangor
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Manually annotated by BRENDA team
an acrylamide-degrading bacterium isolated from the grounds of a palm estate in Serdang, Selangor; previously isolated from the grounds of a palm estate in Serdang, Selangor
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
Duch, tissues of aseptically grown squash
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
strain AE1
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Manually annotated by BRENDA team
strain KPO-2771-4
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Manually annotated by BRENDA team
strain MA52
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
dogfish
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
strain JM109 harboring pALJ30 and pALJ20
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Manually annotated by BRENDA team
strain RB791 and HB101
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
isolated from Manikaran thermal spring (Himachal Pradesh, India)
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
strain CBS 498-74
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Manually annotated by BRENDA team
strain CBS 498-74
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
isolated from mineral soil collected from the Miers Valley, McMurdo region, eastern Antarctica
UniProt
Manually annotated by BRENDA team
isolated from mineral soil collected from the Miers Valley, McMurdo region, eastern Antarctica
UniProt
Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
strain T12
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Manually annotated by BRENDA team
strain T12
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
gene pamH
UniProt
Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
strain OMGS 100 and H66
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
protozoa
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
strain AI3, AIU1N and OUCH4; strain AI3 and the mutant strains AIU1N and OUCH4
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Manually annotated by BRENDA team
Pseudomonas aeruginosa PAC 111 (C11)
strain PAC 111 (C11)
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Manually annotated by BRENDA team
Pseudomonas aeruginosa PAC 142 (L10R)
strain PAC 142 (L10R)
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Manually annotated by BRENDA team
PAC142
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Manually annotated by BRENDA team
strain B23
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Manually annotated by BRENDA team
strain 5B
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Manually annotated by BRENDA team
isolated from rhizosphere of cyanogenic plant Circium vulgare
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Manually annotated by BRENDA team
strain PP3 and PPW3
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Manually annotated by BRENDA team
strain W13
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
DSM 9174
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
strain MP50
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Manually annotated by BRENDA team
sequence identical to the sequenced enzyme fragment from strain NCIMB11540; NCIMB11540
SwissProt
Manually annotated by BRENDA team
Rhodococcus erythropolis No. 7
strain No. 7
UniProt
Manually annotated by BRENDA team
strain IFO 15564
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Manually annotated by BRENDA team
strain J1
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Manually annotated by BRENDA team
strain AA21
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Manually annotated by BRENDA team
strain R312
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Manually annotated by BRENDA team
strain S13-4
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
shrimp
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
snake
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
gene azl13
UniProt
Manually annotated by BRENDA team
strain 7, gene ST0478
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Manually annotated by BRENDA team
strain 7, gene ST0478
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
Torula cremoris
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
trout
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
yeast-like fungi
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
metabolism
RhAmidase belongs to amidase signature family, a group of amidase families, and is responsible for the degradation of amides produced from nitriles by nitrile hydratase
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-(+)-2-chloropropionamide + H2O
(+)-2-chloropropionic acid + NH3
show the reaction diagram
(R)-2,2-dimethyl cyclopropanecarboxamide + H2O
?
show the reaction diagram
(R)-2,2-dimethyl cyclopropanecarboxamide + H2O
propanecarboxylic acid + NH3
show the reaction diagram
(R)-2-chloromandelamide + H2O
(R)-2-chloromandelic acid + NH3
show the reaction diagram
(R)-lactamide + H2O
lactate + NH3
show the reaction diagram
about 30% of the activity with (S)-lactamide
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?
(R,S)-2-(3-benzoylphenyl)propanamide + H2O
(S)-2-(3-benzoylphenyl)propanoic acid + NH3
show the reaction diagram
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enantioselective for the S-enantiomer
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?
(R,S)-2-phenylpropionamide + H2O
(S)-2-phenylpropionic acid + (R)-2-phenylpropionamide + NH3
show the reaction diagram
(R,S)-2-phenylpropionamide + H2O
(S)-2-phenylpropionic acid + NH3
show the reaction diagram
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the enzyme is highly S-stereoselective for 2-phenylpropionamide, and the racemic amide is converted to the corresponding S-acid with an enantiomeric excess of over 95% at 50% conversion of the substrate
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?
(R,S)-ketoprofen amide + H2O
(S)-ketoprofen + NH3
show the reaction diagram
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the enzyme hydrolyzes S-ketoprofen amide to its corresponding acid in an absolutely enantioselective manner
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?
(R/S)-2,2-dimethylcyclopropanecarboxamide + H2O
(R)-2,2-dimethylcyclopropanecarboxylic acid + (S)-2,2-dimethylcyclopropanecarboxyamide
show the reaction diagram
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(R/S)-piperazine-2-carboxamide + H2O
(S)-piperazine-2-carboxylic acid + (R)-piperazine-2-carboxamide
show the reaction diagram
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(S)-(-)-2-chloropropionamide + H2O
(-)-2-chloropropionic acid + NH3
show the reaction diagram
(S)-(-)-2-hydroxy-2-(trifluoromethyl)-butanamide + H2O
(R)-(+)-2-hydroxy-2-(trifluoromethyl)-butanoic acid + NH3
show the reaction diagram
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stereospecific reaction
enantiomerically pure product
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?
(S)-(-)-3,3,3-trifluoro-2-amino-2-methylpropanamide + H2O
(R)-(+)-3,3,3-trifluoro-2-amino-2-methylpropanoic acid + NH3
show the reaction diagram
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stereospecific reaction
enantiomerically pure product
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?
(S)-(-)-3,3,3-trifluoro-2-hydroxy-2-methylpropanamide + H2O
(R)-(+)-3,3,3-trifluoro-2-hydroxy-2-methylpropanoic acid + NH3
show the reaction diagram
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stereospecific reaction
enantiomerically pure product
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?
(S)-2,2-dimethyl cyclopropanecarboxamide + H2O
propanecarboxylic acid
show the reaction diagram
(S)-2,2-dimethyl cyclopropanecarboxamide + H2O
propanecarboxylic acid + NH3
show the reaction diagram
(S)-2-(3-benzoylphenyl)propanamide + H2O
(S)-2-(3-benzoylphenyl)propanoic acid + NH3
show the reaction diagram
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ketoprofen amide, R-amide is not converted
ketoprofen
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?
(S)-2-phenylpropionamide + H2O
(S)-2-phenylpropionate + NH3
show the reaction diagram
no activity with (R)-2-phenylpropionamide
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?
(S)-ibuprofen amide + H2O
(S)-ibuprofen + NH3
show the reaction diagram
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?
(S)-lactamide + H2O
lactate + NH3
show the reaction diagram
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?
1-naphthalene-acetamide + H2O
1-naphthaleneacetic acid + NH3
show the reaction diagram
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about 60% of the activity with indole-3-acetamide
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?
1-naphthaleneacetamide + H2O
1-naphthaleneacetic acid + NH3
show the reaction diagram
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?
2 indole-3-acetonitrile + 3 H2O
indole-3-acetamide + indole-3-acetic acid + NH3
show the reaction diagram
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?
2',6'-dimethylacetanilide + H2O
2,6-dimethylaniline+ acetate
show the reaction diagram
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?
2,2-dimethylcyclopropanecarboxamide + H2O
(R)-2,2-dimethylcyclopropanecarboxylic acid + NH3
show the reaction diagram
2-aminobenzamide + H2O
2-aminobenzoic acid + NH3
show the reaction diagram
2-arachidonoylglycerol + H2O
?
show the reaction diagram
2-arachidonoylglycerol + H2O
arachidonic acid + glycerol
show the reaction diagram
2-arylpropionic amide + H2O
(S)-2-arylpropionic acid + NH3
show the reaction diagram
2-arylpropionic amide + H2O
S-2-arylpropionic acid + NH3
show the reaction diagram
2-chloroacetamide + H2O
2-chloroacetate + NH3
show the reaction diagram
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?
2-chloropropionamide + H2O
2-chloropropionate + NH3
show the reaction diagram
2-hydroxybenzamide + H2O
2-hydroxybenzoic acid + NH3
show the reaction diagram
2-nitroacetanilide
2-nitrophenylamine + acetate
show the reaction diagram
2-thiophenecarboxamide + H2O
2-thiophenecarboxylate + NH3
show the reaction diagram
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?
2-toluamide + H2O
2-toluic acid + NH3
show the reaction diagram
2-tolylamide + H2O
? + NH3
show the reaction diagram
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?
3',4'-dichloropropionanilide + H2O
2,3-dichloroanilide + propanoate
show the reaction diagram
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?
3,3,3-trifluoro-2-hydroxy-2-methylpropanamide + H2O
3,3,3-trifluoro-2-hydroxy-2-methylpropanoic acid + NH3
show the reaction diagram
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?
3-hydroxymyristoyl lipid A + H2O
?
show the reaction diagram
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3-hydroxymyristoyl group linked to the proximal glucosamine residue of de-O-acylated lipid A, hydolysis by isozyme FAAI
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?
3-indolacetamide + H2O
3-indolacetate + NH3
show the reaction diagram
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?
3-indolylacetamide + H2O
3-indolylacetic acid + NH3
show the reaction diagram
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-
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?
3-phenyl-propionamide + H2O
3-phenyl-propionate + NH3
show the reaction diagram
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-
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?
3-phenylpropionamide + H2O
3-phenylpropionate + NH3
show the reaction diagram
3-toluamide + H2O
3-toluic acid + NH3
show the reaction diagram
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?
3-tolylamide + H2O
? + NH3
show the reaction diagram
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-
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?
4'-methylacetanilide + H2O
4-methylaniline + acetate
show the reaction diagram
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-
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?
4-aminobenzamide + H2O
4-aminobenzoic acid + NH3
show the reaction diagram
4-chloro-3-hydroxybutyramide + H2O
4-chloro-3-hydroxybutyrate + NH3
show the reaction diagram
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substrate of the soluble enzyme and of the immobilized, cross-linked enzyme
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?
4-chloro-3-hydroxybutyramide + H2O
4-chloro-3-hydroxybutyric acid + NH3
show the reaction diagram
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4-guanidinobutyramide + H2O
?
show the reaction diagram
4-hydroxybenzamide + H2O
4-hydroxybenzoate + NH3
show the reaction diagram
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-
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?
4-hydroxybenzamide + H2O
4-hydroxybenzoic acid + NH3
show the reaction diagram
4-methylbenzamide + H2O
4-methylbenzoic acid + NH3
show the reaction diagram
4-nitroacetanilide + H2O
4-nitroaniline + acetate
show the reaction diagram
4-nitroacetanilide + H2O
acetic acid + 4-nitroaniline
show the reaction diagram
4-nitrobenzamide + H2O
4-nitrobenzoate + NH3
show the reaction diagram
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-
-
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?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
show the reaction diagram