Information on EC 3.5.1.4 - amidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY
3.5.1.4
-
RECOMMENDED NAME
GeneOntology No.
amidase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
a monocarboxylic acid amide + H2O = a monocarboxylate + NH3
show the reaction diagram
stereospecific hydrolysis of 2-arylpropionamides
-
a monocarboxylic acid amide + H2O = a monocarboxylate + NH3
show the reaction diagram
hydrolysis of ester and aromatic amide
-
a monocarboxylic acid amide + H2O = a monocarboxylate + NH3
show the reaction diagram
hydrolysis of aliphatic amide; ping-pong mechanism, substitution mechanism
-
a monocarboxylic acid amide + H2O = a monocarboxylate + NH3
show the reaction diagram
-
-
-
-
a monocarboxylic acid amide + H2O = a monocarboxylate + NH3
show the reaction diagram
catalytic role of an arginine dyad, the catalytic motif is revealed in the model structure of amide hydrolyzing antibody 312D6, the antibody induces catalysis by transition-state mimicry and torsional activation, and accelerates the hydrolysis of amides by a factor of 1000, overview
-
a monocarboxylic acid amide + H2O = a monocarboxylate + NH3
show the reaction diagram
the enzyme contains a nitrilase catalytic triad at the bottom of a 13-A deep, funnel-shaped pocket, accessible from the solvent through a narrow neck with 3-A diameter
-
a monocarboxylic acid amide + H2O = a monocarboxylate + NH3
show the reaction diagram
amino acid residues Ser171, Ser195 and Lys96 are essential for catalytic activity, molecular mechanism of substrate recognition, overview
Q7DKE4
a monocarboxylic acid amide + H2O = a monocarboxylate + NH3
show the reaction diagram
hydrolysis of ester and aromatic amide
Delftia acidovorans AE1
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
carboxylic acid amide hydrolysis
-
-
hydrolysis of amide bond
-
-
hydrolysis of carboxylic acid amide
-
-
-
-
hydrolysis of carboxylic acid amide
-
-
hydrolysis of carboxylic acid amide
-
-
hydrolysis of carboxylic acid amide
-
-
hydrolysis of carboxylic acid amide
-
-
hydrolysis of carboxylic acid amide
-
-
hydrolysis of carboxylic acid amide
-
-
hydrolysis of carboxylic acid amide
Q70I53
-
hydrolysis of carboxylic acid amide
-
-
hydrolysis of carboxylic acid amide
Alcaligenes sp. FB188, Pseudomonas aeruginosa 8602, Rhodococcus sp. R312, Sulfolobus solfataricus MT4
-
-
-
transfer of acyl group
-
-
-
-
transfer of acyl group
-
-
transfer of acyl group
-
-
transfer of acyl group
-
-
transfer of acyl group
-
-
transfer of acyl group
-
-
transfer of acyl group
-
-
transfer of acyl group
Q70I53
-
transfer of acyl group
-
-
transfer of acyl group
Alcaligenes sp. FB188, Pseudomonas aeruginosa 8602, Rhodococcus sp. R312, Sulfolobus solfataricus MT4
-
-
-
transfer of amide group
-
-
-
-
transfer of amide group
-
-
transfer of amide group
-
-
transfer of amide group
-
-
transfer of amide group
-
-
transfer of amide group
-
-
transfer of amide group
-
-
transfer of amide group
Q70I53
-
transfer of amide group
-
-
transfer of amide group
Alcaligenes sp. FB188, Pseudomonas aeruginosa 8602, Rhodococcus sp. R312, Sulfolobus solfataricus MT4
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
acrylonitrile degradation I
-
arginine degradation X (arginine monooxygenase pathway)
-
indole-3-acetate biosynthesis II
-
indole-3-acetate biosynthesis III (bacteria)
-
indole-3-acetate biosynthesis IV (bacteria)
-
Arginine and proline metabolism
-
Phenylalanine metabolism
-
Tryptophan metabolism
-
Aminobenzoate degradation
-
Styrene degradation
-
Microbial metabolism in diverse environments
-
SYSTEMATIC NAME
IUBMB Comments
acylamide amidohydrolase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
A-amidase
-
specificity towards aliphatic amides
acetamidase
-
-
acetamidase
Brevibacterium sp. R312
-
-
-
acid transferase
-
-
-
-
acylamidase
-
-
-
-
acylase
-
-
-
-
amidase
B4XEY3
-
amidase
P22984
-
amidase
Rhodococcus erythropolis No. 7
B4XEY3
-
-
amide hydrolase
-
-
-
-
amide transferase
-
-
-
-
amidohydrolase
-
-
-
-
AmiE
F8AEP0
-
-
anandamide hydrolase
-
-
C-amidase
-
specificity towards chlorinated aliphatic amides
C-amidase
Pseudomonas putida PP3
-
specificity towards chlorinated aliphatic amides
-
deaminase
-
-
-
-
ester transferase
-
-
-
-
FAAH
-
-
FAAH
Rattus norvegicus Sprague-Dawley
-
-
-
FAAH-1
O00519
-
FAAH-1
O08914
-
FAAH-1
P97612
-
FAAH-1
Q9TUI8
-
FAAH-2
Q6GMR7
-
fatty acid amide hydrolase
-
-
fatty acid amide hydrolase
O00519, Q6GMR7
-
fatty acid amide hydrolase
-
-
fatty acid amide hydrolase
-
-
fatty acid amide hydrolase
Rattus norvegicus Sprague-Dawley
-
-
-
fatty acid amide hydrolase 1
O00519
-
fatty acid amide hydrolase 1
O08914
-
fatty acid amide hydrolase 1
P97612
-
fatty acid amide hydrolase 1
Q9TUI8
-
fatty acylamidase
-
with FAA I and FAA II
half-amidase
Blastobacter sp.
-
for half-amide hydrolysis
indole-3-acetamide amidohydrolase
-
-
murein peptide amidase A
-
-
mycothiol-S-conjugate amidase
-
-
N-acetylaminohydrolase
-
-
-
-
N-acylethanolamine acid amidase
-
-
N-acylethanolaminehydrolyzing acid amidase
Q02083
-
N-acylethanolaminehydrolyzing acid amidase
Q9D7V9
-
N-acylethanolaminehydrolyzing acid amidase
Q5KTC7
-
NAAA
Q02083
-
NAAA
Q9D7V9
-
NAAA
Q5KTC7
-
PYCH_10400
F8AEP0
locus name
PYCH_10400
F8AEP0
locus name
-
R-stereospecific amidase
-
-
R-stereospecific amidase
Brevibacterium epidermidis ZJB-07021
-
-
-
RhAmidase
Q7DKE4
-
signature amidase
-
-
signature amidase
P95896
-
signature amidase
P95896
-
-
SsAH
P95896
-
-
SSO2122
P95896
ordered locus name
SSO2122
P95896
ordered locus name
-
tissue kallikrein
-
-
Wide spectrum amidase
-
-
-
-
additional information
Q5YFS2
the enzyme belongs to the amidase signature superfamily
additional information
-
the enzyme belongs to the thiol nitrilases family
additional information
Q7DKE4
RhAmidase belongs to amidase signature, AS, family, a group of amidase families
CAS REGISTRY NUMBER
COMMENTARY
9012-56-0
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain RAPc8, recombinantly expressed in Escherichia coli
-
-
Manually annotated by BRENDA team
Aeribacillus pallidus RAPc8
strain RAPc8
-
-
Manually annotated by BRENDA team
Aeribacillus pallidus RAPc8
strain RAPc8, recombinantly expressed in Escherichia coli
-
-
Manually annotated by BRENDA team
strain FB188, or Bordetella sp.
SwissProt
Manually annotated by BRENDA team
Alcaligenes sp. FB188
strain FB188, or Bordetella sp.
SwissProt
Manually annotated by BRENDA team
Blastobacter sp.
strain A17p-4
-
-
Manually annotated by BRENDA team
strain ZJB-07021
-
-
Manually annotated by BRENDA team
Brevibacterium epidermidis ZJB-07021
strain ZJB-07021
-
-
Manually annotated by BRENDA team
mutant strains A4 and 19
-
-
Manually annotated by BRENDA team
mutant strains A4 and 19; strain R312
-
-
Manually annotated by BRENDA team
Brevibacterium sp. R312
strain R312
-
-
Manually annotated by BRENDA team
strain AM10-C-1
-
-
Manually annotated by BRENDA team
Brevundimonas diminuta AM10-C-1
strain AM10-C-1
-
-
Manually annotated by BRENDA team
strain C, hospital isolate
-
-
Manually annotated by BRENDA team
Burkholderia cepacia C
strain C, hospital isolate
-
-
Manually annotated by BRENDA team
an acrylamide-degrading bacterium isolated from the grounds of a palm estate in Serdang, Selangor
-
-
Manually annotated by BRENDA team
an acrylamide-degrading bacterium isolated from the grounds of a palm estate in Serdang, Selangor
-
-
Manually annotated by BRENDA team
Duch, tissues of aseptically grown squash
-
-
Manually annotated by BRENDA team
strain KPO-2771-4
-
-
Manually annotated by BRENDA team
Delftia acidovorans AE1
strain AE1
-
-
Manually annotated by BRENDA team
Delftia acidovorans KPO-2771-4
strain KPO-2771-4
-
-
Manually annotated by BRENDA team
strain MA52
-
-
Manually annotated by BRENDA team
Delftia tsuruhatensis MA52
strain MA52
-
-
Manually annotated by BRENDA team
strain JM109 harboring pALJ30 and pALJ20
-
-
Manually annotated by BRENDA team
strain RB791 and HB101
-
-
Manually annotated by BRENDA team
Escherichia coli JM109
strain JM109 harboring pALJ30 and pALJ20
-
-
Manually annotated by BRENDA team
Escherichia coli RB791
strain RB791 and HB101
-
-
Manually annotated by BRENDA team
from COS-7 cells transfected with human FAAH-1
SwissProt
Manually annotated by BRENDA team
healthy male individuals
-
-
Manually annotated by BRENDA team
recombinantly expressed in COS-7 cells
-
-
Manually annotated by BRENDA team
wild-type is strain MM6, mutant strains are MM8 and MM15
-
-
Manually annotated by BRENDA team
strain CBS 498-74
-
-
Manually annotated by BRENDA team
Microbacterium imperiale CBS 498-74
strain CBS 498-74
-
-
Manually annotated by BRENDA team
Nesterenkonia sp.
isolated from mineral soil collected from the Miers Valley, McMurdo region, eastern Antarctica
UniProt
Manually annotated by BRENDA team
isolated from mineral soil collected from the Miers Valley, McMurdo region, eastern Antarctica
UniProt
Manually annotated by BRENDA team
strain T12
-
-
Manually annotated by BRENDA team
Ochrobactrum sp. T12
strain T12
-
-
Manually annotated by BRENDA team
strain OMGS 100 and H66
-
-
Manually annotated by BRENDA team
Porphyromonas gingivalis OMGS 100
strain OMGS 100 and H66
-
-
Manually annotated by BRENDA team
; gene amiE, inducible enzyme
-
-
Manually annotated by BRENDA team
C166S and C166A mutants; mutant strain KLAM59
-
-
Manually annotated by BRENDA team
gene amiE, inducible enzyme
-
-
Manually annotated by BRENDA team
mutant strain KLAM59; mutant strain Ph1
-
-
Manually annotated by BRENDA team
mutant strains B6, AI3, AIU1N, OUCH and L10
-
-
Manually annotated by BRENDA team
PAU3 and PAU5
-
-
Manually annotated by BRENDA team
strain AI3, AIU1N and OUCH4; strain AI3 and the mutant strains AIU1N and OUCH4
-
-
Manually annotated by BRENDA team
strain PAC 111 (C11); strain PAC 142 (L10R)
-
-
Manually annotated by BRENDA team
strain PAC 142 (L10R)
-
-
Manually annotated by BRENDA team
Pseudomonas aeruginosa 8602
gene amiE
-
-
Manually annotated by BRENDA team
Pseudomonas aeruginosa 8602
strain 8602
-
-
Manually annotated by BRENDA team
Pseudomonas aeruginosa AI3
strain AI3, AIU1N and OUCH4; strain AI3 and the mutant strains AIU1N and OUCH4
-
-
Manually annotated by BRENDA team
Pseudomonas aeruginosa PAC 111 (C11)
strain PAC 111 (C11)
-
-
Manually annotated by BRENDA team
Pseudomonas aeruginosa PAC 142 (L10R)
strain PAC 142 (L10R)
-
-
Manually annotated by BRENDA team
Pseudomonas aeruginosa PAC142
PAC142
-
-
Manually annotated by BRENDA team
Pseudomonas chlororaphis B23
strain B23
-
-
Manually annotated by BRENDA team
strain 5B
-
-
Manually annotated by BRENDA team
strain PP3 and PPW3
-
-
Manually annotated by BRENDA team
Pseudomonas putida 5B
strain 5B
-
-
Manually annotated by BRENDA team
Pseudomonas putida PP3
strain PP3 and PPW3
-
-
Manually annotated by BRENDA team
strain W13
-
-
Manually annotated by BRENDA team
strain W13
-
-
Manually annotated by BRENDA team
thermophilic actinomycete, DSMZ 43832
-
-
Manually annotated by BRENDA team
adult Sprague-Dawley rats
-
-
Manually annotated by BRENDA team
expressed in Escherichia coli
-
-
Manually annotated by BRENDA team
recombinantly expressed in Escherichia coli
-
-
Manually annotated by BRENDA team
Sprague-Dawley rats
-
-
Manually annotated by BRENDA team
sequence identical to the sequenced enzyme fragment from strain NCIMB11540; NCIMB11540
SwissProt
Manually annotated by BRENDA team
strain MTCC 1526, Rhodococcus erythropolis strain MTCC 1548 and Rhodococcus sp. strains MTCC 2574, 2678, 2683, 2794 and 3951 produce also amidase
-
-
Manually annotated by BRENDA team
strain No. 7
UniProt
Manually annotated by BRENDA team
Rhodococcus erythropolis MP50
strain MP50
-
-
Manually annotated by BRENDA team
Rhodococcus erythropolis NCIMB11540
sequence identical to the sequenced enzyme fragment from strain NCIMB11540; NCIMB11540
SwissProt
Manually annotated by BRENDA team
Rhodococcus erythropolis No. 7
strain No. 7
UniProt
Manually annotated by BRENDA team
strain IFO 15564
-
-
Manually annotated by BRENDA team
Rhodococcus rhodochrous IFO 15564
strain IFO 15564
-
-
Manually annotated by BRENDA team
Rhodococcus rhodochrous J1
strain J1
-
-
Manually annotated by BRENDA team
strain AA21
-
-
Manually annotated by BRENDA team
strain R312
-
-
Manually annotated by BRENDA team
strain S13-4
-
-
Manually annotated by BRENDA team
Rhodococcus sp. AA21
strain AA21
-
-
Manually annotated by BRENDA team
strain R312
-
-
Manually annotated by BRENDA team
Rhodococcus sp. S13-4
strain S13-4
-
-
Manually annotated by BRENDA team
bacteriophage isolated from Pneumococcus sp., strain 101/87
-
-
Manually annotated by BRENDA team
wild-type strain R6
-
-
Manually annotated by BRENDA team
Sulfolobus solfataricus MT4
-
SwissProt
Manually annotated by BRENDA team
Sulfolobus solfataricus MT4
MT4 strain
-
-
Manually annotated by BRENDA team
strain 7, gene ST0478
-
-
Manually annotated by BRENDA team
Sulfolobus tokodaii 7
strain 7, gene ST0478
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
Torula cremoris
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
metabolism
Q7DKE4
RhAmidase belongs to amidase signature family, a group of amidase families, and is responsible for the degradation of amides produced from nitriles by nitrile hydratase
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-(+)-2-chloropropionamide + H2O
(+)-2-chloropropionic acid + NH3
show the reaction diagram
Escherichia coli, Escherichia coli JM109
-
-
-
?
(R)-2,2-dimethyl cyclopropanecarboxamide + H2O
?
show the reaction diagram
Delftia tsuruhatensis, Delftia tsuruhatensis MA52
-
R-stereospecific avtivity
-
-
?
(R)-2,2-dimethyl cyclopropanecarboxamide + H2O
propanecarboxylic acid + NH3
show the reaction diagram
Ochrobactrum sp., Ochrobactrum sp. T12
-
R-stereospecific avtivity
-
-
?
(R)-2-chloromandelamide + H2O
(R)-2-chloromandelic acid + NH3
show the reaction diagram
Q9EV64, -
-
-
-
?
(R)-2-chloromandelamide + H2O
(R)-2-chloromandelic acid + NH3
show the reaction diagram
Rhodococcus erythropolis NCIMB11540
Q9EV64
-
-
-
?
(R,S)-2-(3-benzoylphenyl)propanamide + H2O
(S)-2-(3-benzoylphenyl)propanoic acid + NH3
show the reaction diagram
-
enantioselective for the S-enantiomer
-
-
?
(R,S)-2-phenylpropionamide + H2O
(S)-2-phenylpropionic acid + (R)-2-phenylpropionamide + NH3
show the reaction diagram
Sulfolobus tokodaii, Sulfolobus tokodaii 7
-
enantioselective reaction with preference for the S-enantiomer
-
-
?
(R,S)-2-phenylpropionamide + H2O
(S)-2-phenylpropionic acid + NH3
show the reaction diagram
-
the enzyme is highly S-stereoselective for 2-phenylpropionamide, and the racemic amide is converted to the corresponding S-acid with an enantiomeric excess of over 95% at 50% conversion of the substrate
-
-
?
(R,S)-ketoprofen amide + H2O
(S)-ketoprofen + NH3
show the reaction diagram
-
the enzyme hydrolyzes S-ketoprofen amide to its corresponding acid in an absolutely enantioselective manner
-
-
?
(R/S)-2,2-dimethylcyclopropanecarboxamide + H2O
(R)-2,2-dimethylcyclopropanecarboxylic acid + (S)-2,2-dimethylcyclopropanecarboxyamide
show the reaction diagram
-
-
-
-
-
(R/S)-piperazine-2-carboxamide + H2O
(S)-piperazine-2-carboxylic acid + (R)-piperazine-2-carboxamide
show the reaction diagram
-
-
-
-
-
(S)-(-)-2-chloropropionamide + H2O
(-)-2-chloropropionic acid + NH3
show the reaction diagram
Escherichia coli, Escherichia coli JM109
-
-
-
?
(S)-(-)-2-hydroxy-2-(trifluoromethyl)-butanamide + H2O
(R)-(+)-2-hydroxy-2-(trifluoromethyl)-butanoic acid + NH3
show the reaction diagram
-
stereospecific reaction
enantiomerically pure product
-
?
(S)-(-)-3,3,3-trifluoro-2-amino-2-methylpropanamide + H2O
(R)-(+)-3,3,3-trifluoro-2-amino-2-methylpropanoic acid + NH3
show the reaction diagram
-
stereospecific reaction
enantiomerically pure product
-
?
(S)-(-)-3,3,3-trifluoro-2-hydroxy-2-methylpropanamide + H2O
(R)-(+)-3,3,3-trifluoro-2-hydroxy-2-methylpropanoic acid + NH3
show the reaction diagram
-
stereospecific reaction
enantiomerically pure product
-
?
(S)-2,2-dimethyl cyclopropanecarboxamide + H2O
propanecarboxylic acid
show the reaction diagram
Rhodococcus sp., Rhodococcus sp. AA21
-
S-stereospecific activity
-
-
?
(S)-2,2-dimethyl cyclopropanecarboxamide + H2O
propanecarboxylic acid + NH3
show the reaction diagram
-
S-stereospecific activity
-
-
?
(S)-2-(3-benzoylphenyl)propanamide + H2O
(S)-2-(3-benzoylphenyl)propanoic acid + NH3
show the reaction diagram
-
ketoprofen amide, R-amide is not converted
ketoprofen
-
?
(S)-2-phenylpropionamide + H2O
(S)-2-phenylpropionate + NH3
show the reaction diagram
P95896
no activity with (R)-2-phenylpropionamide
-
-
?
(S)-ibuprofen amide + H2O
(S)-ibuprofen + NH3
show the reaction diagram
-
-
-
-
?
1-naphthalene-acetamide + H2O
1-naphthaleneacetic acid + NH3
show the reaction diagram
-
about 60% of the activity with indole-3-acetamide
-
-
?
1-naphthaleneacetamide + H2O
1-naphthaleneacetic acid + NH3
show the reaction diagram
-
-
-
?
2 indole-3-acetonitrile + 3 H2O
indole-3-acetamide + indole-3-acetic acid + NH3
show the reaction diagram
-
-
-
?
2,2-dimethylcyclopropanecarboxamide + H2O
(R)-2,2-dimethylcyclopropanecarboxylic acid + NH3
show the reaction diagram
Brevibacterium epidermidis, Brevibacterium epidermidis ZJB-07021
-
70 min of bioconversion at 35C, kinetic resolution of (R,S)-2,2-dimethylcyclopropanecarboxamide by the amidase affords (S)-2,2-dimethylcyclopropanecarboxamide in 41.1% yield (>99% ee) and (R)-2,2-dimethylcyclopropanecarboxylic acid in 49.9% yield (69.7% ee). The enantioselectivity is temperature dependent and is enhanced from 12.6 at 45C to 65.9 at 14C
-
-
?
2-aminobenzamide + H2O
2-aminobenzoic acid + NH3
show the reaction diagram
-
-
-
-
?
2-aminobenzamide + H2O
2-aminobenzoic acid + NH3
show the reaction diagram
P95896
-
-
-
?
2-arachidonoylglycerol + H2O
arachidonic acid + glycerol
show the reaction diagram
O00519, Q02083
-
-
-
?
2-arachidonoylglycerol + H2O
arachidonic acid + glycerol
show the reaction diagram
Q9TUI8
-
-
-
?
2-arachidonoylglycerol + H2O
?
show the reaction diagram
-, O08914, Q9D7V9
-
-
-
?
2-arachidonoylglycerol + H2O
?
show the reaction diagram
P97612, Q5KTC7
-
-
-
?
2-arylpropionic amide + H2O
(S)-2-arylpropionic acid + NH3
show the reaction diagram
Delftia acidovorans, Delftia acidovorans KPO-2771-4
-
enantioselective S-amidase
-
?
2-arylpropionic amide + H2O
S-2-arylpropionic acid + NH3
show the reaction diagram
Delftia acidovorans, Delftia acidovorans KPO-2771-4
-
enantioselective S-amidase
-
?
2-chloroacetamide + H2O
2-chloroacetate + NH3
show the reaction diagram
-
-
-
-
?
2-chloropropionamide + H2O
2-chloropropionate + NH3
show the reaction diagram
Pseudomonas putida, Pseudomonas putida PP3
-
-
-
?
2-hydroxybenzamide + H2O
2-hydroxybenzoic acid + NH3
show the reaction diagram
-
-
-
-
?
2-hydroxybenzamide + H2O
2-hydroxybenzoic acid + NH3
show the reaction diagram
P95896
-
-
-
?
2-nitroacetanilide
2-nitrophenylamine + acetate
show the reaction diagram
Delftia acidovorans, Delftia acidovorans AE1
-
-
-
?
2-thiophenecarboxamide + H2O
2-thiophenecarboxylate + NH3
show the reaction diagram
P95896
-
-
-
?
2-toluamide + H2O
2-toluic acid + NH3
show the reaction diagram
-
-
-
-
?
2-toluamide + H2O
2-toluic acid + NH3
show the reaction diagram
P95896
-
-
-
?
2-toluamide + H2O
2-toluic acid + NH3
show the reaction diagram
-
-
-
-
?
2-tolylamide + H2O
? + NH3
show the reaction diagram
-
-
-
-
?
3,3,3-trifluoro-2-hydroxy-2-methylpropanamide + H2O
3,3,3-trifluoro-2-hydroxy-2-methylpropanoic acid + NH3
show the reaction diagram
-
-
-
-
?
3-hydroxymyristoyl lipid A + H2O
?
show the reaction diagram
-
3-hydroxymyristoyl group linked to the proximal glucosamine residue of de-O-acylated lipid A, hydolysis by isozyme FAAI
-
-
?
3-indolacetamide + H2O
3-indolacetate + NH3
show the reaction diagram
P95896
-
-
-
?
3-indolylacetamide + H2O
3-indolylacetic acid + NH3
show the reaction diagram
-
-
-
-
?
3-phenyl-propionamide + H2O
3-phenyl-propionate + NH3
show the reaction diagram
-
-
-
-
?
3-phenylpropionamide + H2O
3-phenylpropionate + NH3
show the reaction diagram
P95896
-
-
-
?
3-phenylpropionamide + H2O
3-phenylpropionate + NH3
show the reaction diagram
-
-
-
-
?
3-toluamide + H2O
3-toluic acid + NH3
show the reaction diagram
P95896
-
-
-
?
3-tolylamide + H2O
? + NH3
show the reaction diagram
-
-
-
-
?
4-aminobenzamide + H2O
4-aminobenzoic acid + NH3
show the reaction diagram
-
-
-
-
?
4-aminobenzamide + H2O
4-aminobenzoic acid + NH3
show the reaction diagram
P95896
-
-
-
?
4-chloro-3-hydroxybutyramide + H2O
4-chloro-3-hydroxybutyric acid + NH3
show the reaction diagram
B4XEY3
-
-
-
-
4-chloro-3-hydroxybutyramide + H2O
4-chloro-3-hydroxybutyrate + NH3
show the reaction diagram
-
substrate of the soluble enzyme and of the immobilized, cross-linked enzyme
-
-
?
4-hydroxybenzamide + H2O
4-hydroxybenzoic acid + NH3
show the reaction diagram
-
-
-
-
?
4-hydroxybenzamide + H2O
4-hydroxybenzoic acid + NH3
show the reaction diagram
P95896
-
-
-
?
4-hydroxybenzamide + H2O
4-hydroxybenzoate + NH3
show the reaction diagram
-
-
-
-
?
4-methylbenzamide + H2O
4-methylbenzoic acid + NH3
show the reaction diagram
-
-
-
-
?
4-methylbenzamide + H2O
4-methylbenzoic acid + NH3
show the reaction diagram
P95896
best aromatic substrate
-
-
?
4-nitroacetanilide + H2O
acetic acid + 4-nitroaniline
show the reaction diagram
Pseudomonas aeruginosa, Pseudomonas aeruginosa AI3
-
-
-
-
?
4-nitroacetanilide + H2O
4-nitroaniline + acetate
show the reaction diagram
-
-
-
-
?
4-nitroacetanilide + H2O
4-nitroaniline + acetate
show the reaction diagram
Pseudomonas aeruginosa 8602
-
-
-
-
?
4-nitrobenzamide + H2O
4-nitrobenzoate + NH3
show the reaction diagram
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
show the reaction diagram
Delftia acidovorans, Delftia acidovorans AE1
-
-
-
?
4-tolylamide + H2O
? + NH3
show the reaction diagram
-
-
-
-
?
5-[(4-nitrophenyl)amino]-5-oxopentanoic acid + H2O
glutarate + 4-nitroaniline
show the reaction diagram
-, Q5YFS2
-
-
-
?
5-[(5-carboxypentanoyl)amino]-2-nitrobenzoic acid + H2O
2-nitrobenzoate + adipate
show the reaction diagram
-, Q5YFS2
-
-
-
?
acetamide + H2O
acetic acid + NH3
show the reaction diagram
-
-
-
-
?
acetamide + H2O
acetic acid + NH3
show the reaction diagram
-
-
-
-
?
acetamide + H2O
acetic acid + NH3
show the reaction diagram
-
-
-
?
acetamide + H2O
acetic acid + NH3
show the reaction diagram
-
-
-
-
?
acetamide + H2O
acetic acid + NH3
show the reaction diagram
-
-
-
-
?
acetamide + H2O
acetic acid + NH3
show the reaction diagram
-
mutant W138G and T103I
-
-
?
acetamide + H2O
acetic acid + NH3
show the reaction diagram
-
amidase-catalyzed amide formation may cause the remaining of acetamide in the acetonitrile conversion process
-
-
r
acetamide + H2O
acetic acid + NH3
show the reaction diagram
B4XEY3
also acyl transfer activity in the presence of hydroxylamine
-
-
?
acetamide + H2O
acetic acid + NH3
show the reaction diagram
Pseudomonas aeruginosa PAC142
-
-
-
-
?
acetamide + H2O
acetic acid + NH3
show the reaction diagram
Rhodococcus erythropolis No. 7
B4XEY3
also acyl transfer activity in the presence of hydroxylamine
-
-
?
acetamide + H2O
acetic acid + NH3
show the reaction diagram
Pseudomonas aeruginosa AI3
-
-
-
-
?
acetamide + H2O
acetic acid + NH3
show the reaction diagram
P95896
-
-
-
?
acetamide + H2O
acetic acid + NH3
show the reaction diagram
Rhodococcus sp. S13-4
-
amidase-catalyzed amide formation may cause the remaining of acetamide in the acetonitrile conversion process
-
-
r
acetamide + H2O
?
show the reaction diagram
Q9EV64, -
-
-
-
?
acetamide + H2O
?
show the reaction diagram
Rhodococcus erythropolis NCIMB11540
Q9EV64
-
-
-
?
acetamide + H2O
acetate + NH3
show the reaction diagram
-
-
-
-
?
acetamide + H2O
acetate + NH3
show the reaction diagram
-
-
-
-
?
acetamide + H2O
acetate + NH3
show the reaction diagram
-
-
-
-
?
acetamide + H2O
acetate + NH3
show the reaction diagram
-
-
-
-
?
acetamide + H2O
acetate + NH3
show the reaction diagram
-
-
-
-
?
acetamide + H2O
acetate + NH3
show the reaction diagram
-
-
-
-
?
acetamide + H2O
acetate + NH3
show the reaction diagram
-
-
-
-
?
acetamide + H2O
acetate + NH3
show the reaction diagram
Q7DKE4
-
-
-
?
acetamide + H2O
acetate + NH3
show the reaction diagram
P95896
-
-
-
?
acetamide + H2O
acetate + NH3
show the reaction diagram
F8AEP0
-
-
-
?
acetamide + H2O
acetate + NH3
show the reaction diagram
-
substrate of the soluble enzyme and of the immobilized, cross-linked enzyme, low activity
-
-
?
acetamide + H2O
acetate + NH3
show the reaction diagram
Aeribacillus pallidus RAPc8
-
-
-
-
?
acetamide + H2O
acetate + NH3
show the reaction diagram
Brevundimonas diminuta AM10-C-1
-
-
-
-
?
acetamide + H2O
acetate + NH3
show the reaction diagram
Pseudomonas aeruginosa 8602
-
-
-
-
?
acetamide + H2O
acetate + NH3
show the reaction diagram
-
-
-
-
?
acetamide + H2O
acetate + NH3
show the reaction diagram
-
-
-
-
?
acetamide + hydroxylamine
acetylhydroxamic acid + NH3
show the reaction diagram
-
-
-
-
?
acetamide + hydroxylamine
acetylhydroxamic acid + NH3
show the reaction diagram
-
Ping Pong Bi Bi type mechanism
-
-
?
acetanilide + H2O
acetate + aniline
show the reaction diagram
-
-
-
?
acetanilide + H2O
acetate + aniline
show the reaction diagram
-
-
-
-
-
acetanilide + H2O
acetate + aniline
show the reaction diagram
Pseudomonas aeruginosa AI3
-
-
-
?
acetanilide + H2O
acetate + aniline
show the reaction diagram
Delftia acidovorans AE1
-
-
-
-
-
acetate-prelabeled chicken histones + H2O
deacetylated chicken histones + acetate
show the reaction diagram
Alcaligenes sp., Alcaligenes sp. FB188
Q70I53
-
-
-
?
acetohydrazide + H2O
hydrazine + acetate
show the reaction diagram
-
-
-
?
acetohydroxamate + H2O
hydroxylamine + acetate
show the reaction diagram
-
-
-
?
acetylcadaverine + H2O
acetate + cadaverine
show the reaction diagram
Alcaligenes sp., Alcaligenes sp. FB188
Q70I53
-
-
-
?
acetylputrescine + H2O
acetate + putrescine
show the reaction diagram
Alcaligenes sp., Alcaligenes sp. FB188
Q70I53
-
-
-
?
acetylspermidine + H2O
acetate + spermidine
show the reaction diagram
Alcaligenes sp., Alcaligenes sp. FB188
Q70I53
-
-
-
?
acrylamide + H2O
acrylic acid + NH3
show the reaction diagram
-
-
-
?
acrylamide + H2O
acrylic acid + NH3
show the reaction diagram
-
-
-
-
?
acrylamide + H2O
acrylic acid + NH3
show the reaction diagram
F8AEP0
-
-
-
?
acrylamide + H2O
acrylic acid + NH3
show the reaction diagram
-
42% of the activity with acetamide. The activity of the reverse reaction is 3% of the activity with acetic acid
-
-
r
acrylamide + H2O
acrylic acid + NH3
show the reaction diagram
B4XEY3
also acyl transfer activity in the presence of hydroxylamine
-
-
?
acrylamide + H2O
acrylic acid + NH3
show the reaction diagram
F8AEP0, -
-
-
-
?
acrylamide + H2O
acrylic acid + NH3
show the reaction diagram
Rhodococcus erythropolis No. 7
B4XEY3
also acyl transfer activity in the presence of hydroxylamine
-
-
?
acrylamide + H2O
acrylic acid + NH3
show the reaction diagram
Rhodococcus sp. S13-4
-
42% of the activity with acetamide. The activity of the reverse reaction is 3% of the activity with acetic acid
-
-
r
acrylamide + H2O
acrylate + NH3
show the reaction diagram
-
-
-
-
?
acrylamide + H2O
acrylate + NH3
show the reaction diagram
-
-
-
-
?
acrylamide + H2O
acrylate + NH3
show the reaction diagram
-
-
-
-
?
acrylamide + H2O
acrylate + NH3
show the reaction diagram
P95896
-
-
-
?
acrylamide + H2O
acrylate + NH3
show the reaction diagram
Q7DKE4
low activity
-
-
?
acrylamide + H2O
acrylate + NH3
show the reaction diagram
-
102% of the activity with acetamide
-
-
?
acrylamide + H2O
acrylate + NH3
show the reaction diagram
-
substrate of the soluble enzyme and of the immobilized, cross-linked enzyme
-
-
?
acrylamide + H2O
acrylate + NH3
show the reaction diagram
-
-
-
-
?
acrylamide + H2O
prop-2-enoic acid + NH3
show the reaction diagram
-
-
-
-
?
acrylamide + H2O
prop-2-enoic acid + NH3
show the reaction diagram
-
mutant W138G
-
-
?
acrylamide + hydroxylamine
acrylhydroxamic acid + NH3
show the reaction diagram
-
-
-
-
?
acrylamide + hydroxylamine
acryloylhydroxamic acid + NH3
show the reaction diagram
-
-
-
-
?
acylamide + H2O
carboxylic acid + NH3
show the reaction diagram
-
-
-
?
adipoamide + H2O
adipic acid + NH3
show the reaction diagram
-
-
-
-
?
adipyl-4-nitro-aniline + H2O
?
show the reaction diagram
-, Q5YFS2
-
-
-
?
anandamide + H2O
?
show the reaction diagram
-
-
-
-
?
anandamide + H2O
?
show the reaction diagram
-
i.e. arachidonoylethanolamide
-
-
?
anandamide + H2O
?
show the reaction diagram
-
i.e. arachidonoylethanolamide or AEA
-
-
?
anandamide + H2O
?
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
i.e. arachidonoylethanolamide, i.e. arachidonoylethanolamide or AEA
-
-
?
anandamide + H2O
arachidonic acid + ethanolamine
show the reaction diagram
-
-
-
-
?
anandamide + H2O
arachidonic acid + ethanolamine
show the reaction diagram
O00519, Q6GMR7
FAAH-1
-
-
?
anandamide + H2O
arachidonic acid + ethanolamine
show the reaction diagram
O00519, Q6GMR7
FAAH-2
-
-
?
anandamide + H2O
arachidonic acid + ethanolamine
show the reaction diagram
-, O08914, Q9D7V9
substrates in order of decreasing activity: anandamide, N-oleoylethanolamine, N-palmitoylethanolamine
-
-
?
anandamide + H2O
arachidonic acid + ethanolamine
show the reaction diagram
P97612, Q5KTC7
substrates in order of decreasing activity: anandamide, N-oleoylethanolamine, N-palmitoylethanolamine
-
-
?
anandamide + H2O
arachidonic acid + ethanolamine
show the reaction diagram
O00519, Q02083
substrates in order of decreasing activity: anandamide, N-oleoylethanolamine, N-palmitoylethanolamine
-
-
?
anandamide + H2O
arachidonic acid + ethanolamine
show the reaction diagram
Q9TUI8
substrates in order of decreasing activity: anandamide, N-oleoylethanolamine, N-palmitoylethanolamine
-
-
?
anandamide + H2O
arachidonic acid + ethanolamine
show the reaction diagram
O00519, Q02083
substrates in order of decreasing activity: N-palmitoylethanolamine, N-oleoylethanolamine, anandamide
-
-
?
anandamide + H2O
arachidonic acid + ethanolamine
show the reaction diagram
P97612, Q5KTC7
substrates in order of decreasing activity: N-palmitoylethanolamine, N-oleoylethanolamine, anandamide
-
-
?
anandamide + H2O
arachidonic acid + ethanolamine
show the reaction diagram
-, O08914, Q9D7V9
substrates in order of decreasing activity: N-palmitoylethanolamine, N-oleoylethanolamine, anandamide
-
-
?
anthranilamide + H2O
anthranilate + NH3
show the reaction diagram
P95896
-
-
-
?
arachidonoyl-ethanolamine + H2O
arachidonic acid + ethanolamine
show the reaction diagram
-
hydrolysis of anandamide
-
-
?
arachidonoyl-ethanolamine + H2O
arachidonic acid + ethanolamine
show the reaction diagram
-
hydrolysis of anandamide
-
-
?
benzamide
benzoic acid + NH3
show the reaction diagram
Blastobacter sp.
-
-
-
?
benzamide + H2O
benzoic acid + NH3
show the reaction diagram
-
-
-
-
-
benzamide + H2O
benzoic acid + NH3
show the reaction diagram
-
-
-
?
benzamide + H2O
benzoic acid + NH3
show the reaction diagram
-
-
-
-
?
benzamide + H2O
benzoic acid + NH3
show the reaction diagram
-
-
-
-
?
benzamide + H2O
benzoic acid + NH3
show the reaction diagram
-
-
-
-
?
benzamide + H2O
benzoic acid + NH3
show the reaction diagram
B4XEY3
also acyl transfer activity in the presence of hydroxylamine
-
-
?
benzamide + H2O
benzoic acid + NH3
show the reaction diagram
Sulfolobus tokodaii 7
-
-
-
-
?
benzamide + H2O
benzoic acid + NH3
show the reaction diagram
Rhodococcus erythropolis No. 7
B4XEY3
also acyl transfer activity in the presence of hydroxylamine
-
-
?
benzamide + H2O
benzoic acid + NH3
show the reaction diagram
Escherichia coli JM109
-
-
-
?
benzamide + H2O
benzoic acid + NH3
show the reaction diagram
Escherichia coli JM109
-
-
-
-
-
benzamide + H2O
benzoate + NH3
show the reaction diagram
-
-
-
-
?
benzamide + H2O
benzoate + NH3
show the reaction diagram
P95896
-
-
-
?
benzamide + H2O
benzoate + NH3
show the reaction diagram
-
-
-
-
?
benzamide + H2O
benzoate + NH3
show the reaction diagram
Q7DKE4
preferred substrate
-
-
?
benzamide + H2O
benzoate + NH3
show the reaction diagram
-
substrate of the soluble enzyme and of the immobilized, cross-linked enzyme
-
-
?
benzamide + H2O
benzoate + NH3
show the reaction diagram
-
-
-
-
?
benzamide + hydroxylamine
benzoylhydroxamic acid + NH3
show the reaction diagram
-
-
-
-
?
Boc-Lys(epsilon-acetyl)-(4-methyl-coumaryl-7-amide) + H2O
Boc-Lys-(4-methyl-coumaryl-7-amide) + acetate
show the reaction diagram
Alcaligenes sp., Alcaligenes sp. FB188
Q70I53
-
-
-
?
butyramide + H2O
n-butyrate + NH3
show the reaction diagram
-
-
-
?
butyramide + H2O
butyric acid + NH3
show the reaction diagram
B4XEY3
-
-
-
?
butyramide + H2O
butyrate + NH3
show the reaction diagram
-
-
-
-
?
butyramide + H2O
butyrate + NH3
show the reaction diagram
-
-
-
-
?
butyramide + H2O
butyrate + NH3
show the reaction diagram
P95896
-
-
-
?
butyramide + H2O
butyrate + NH3
show the reaction diagram
-
-
-
-
?
butyramide + H2O
butyrate + NH3
show the reaction diagram
-
substrate of the soluble enzyme and of the immobilized, cross-linked enzyme
-
-
?
butyramide + H2O
butyrate + NH3
show the reaction diagram
P95896
-
-
-
?
butyramide + hydroxylamine
butyrylhydroxamic acid + NH3
show the reaction diagram
-
-
-
-
?
carboxylic acid + hydroxylamine
N-hydroxy-carboxylic acid amide + H2O
show the reaction diagram
Brevibacterium sp., Brevibacterium sp. R312
-
acid transferase transferase activity
-
?
carboxylic acid amide + H2O
carboxylic acid + NH3
show the reaction diagram
Brevibacterium sp., Brevibacterium sp. R312
-
-
-
?
carboxylic acid amide + hydroxylamine
N-hydroxy-carboxylic acid amide + NH3
show the reaction diagram
Brevibacterium sp., Brevibacterium sp. R312
-
amide transferase activity
-
?
carboxylic acid ester + hydroxylamine
N-hydroxy-carboxylic acid amide + alcohol
show the reaction diagram
Brevibacterium sp., Brevibacterium sp. R312
-
ester transferase activity
-
?
cinnamamide + H2O
cinnamic acid + NH3
show the reaction diagram
-
-
-
-
?
cis-(1S,4R)-N-(4-(hydroxymethyl)cyclopent-2-enyl)acetamide + H2O
cis-(1S,4R)-(4-(hydroxymethyl)cyclopent-2-enyl)amine + acetate
show the reaction diagram
Q70I53
used as enzymatic assay
-
-
?
crotonic acid + NH3
crotonamide + H2O
show the reaction diagram
-
5% of the activity with acetic acid. Reaction with crotonamide is 0.3% of the activity with acetamide
-
-
r
cyclohexanamide + H2O
cyclohexanoate + NH3
show the reaction diagram
P95896
-
-
-
?
cyclohexanoamide + H2O
cyclohexanoic acid + NH3
show the reaction diagram
-
-
-
-
?
D-lactamide + H2O
D-lactate + NH3
show the reaction diagram
-
17% of the activity with acetamide, D-selectivity towards lactamide
-
-
?
D-lactamide + hydroxylamine
?
show the reaction diagram
-
-
-
-
?
D-Val-L-Leu-L-Arg 4-nitroanilide + H2O
D-Val-L-Leu-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
diacetamide + H2O
?
show the reaction diagram
-
14% of the activity with acetamide
-
-
?
DL-lactamide + H2O
DL-lactate + NH3
show the reaction diagram
-
-
-
?
DL-lactamide + H2O
DL-lactate + NH3
show the reaction diagram
-
-
-
-
?
DL-lactamide + H2O
DL-lactate + NH3
show the reaction diagram
Brevibacterium sp. R312
-
-
-
?
DL-methioninamide + H2O
DL-methionine + NH3
show the reaction diagram
-
-
-
-
?
DL-tryptophanamide + H2O
DL-tryptophan + NH3
show the reaction diagram
-
-
-
-
?
ethyl acetate + H2O
ethanol + acetate
show the reaction diagram
-
-
-
?
ethyl acetate + H2O
acetic acid + ethaneamine
show the reaction diagram
Pseudomonas aeruginosa, Pseudomonas aeruginosa PAC142
-
-
-
-
?
ethyl acrylate + H2O
acrylate + ethanol
show the reaction diagram
-
-
-
?
ethyl propionate + H2O
ethanol + propionate
show the reaction diagram
-
-
-
?
fluoroacetamide + H2O
fluoroacetate + NH3
show the reaction diagram
-
165% of the activity with acetamide
-
-
?
formamide + H2O
formate + NH3
show the reaction diagram
-
-
-
-
?
formamide + H2O
formate + NH3
show the reaction diagram
-
30% of the activity with acetamide
-
-
?
formamide + H2O
formate + NH3
show the reaction diagram
-
99% of the activity with acetamide
-
-
?
formamide + H2O
formate + NH3
show the reaction diagram
F8AEP0
22.5% of the activity with acetamide
-
-
?
formamide + H2O
formate + NH3
show the reaction diagram
Aeribacillus pallidus RAPc8
-
30% of the activity with acetamide
-
-
?
formamide + H2O
formate + NH3
show the reaction diagram
F8AEP0, -
22.5% of the activity with acetamide
-
-
?
formamide + H2O
formate + NH3
show the reaction diagram
Rhodococcus sp. S13-4
-
99% of the activity with acetamide
-
-
?
glutaramic acid + H2O
glutaric acid + NH3
show the reaction diagram
Blastobacter sp.
-
-
-
?
glutaryl-2-naphthylamine + H2O
?
show the reaction diagram
-, Q5YFS2
-
-
-
?
glutaryl-aniline + H2O
?
show the reaction diagram
-, Q5YFS2
-
-
-
?
glutaryl-nitro-5-amino benzoic acid + H2O
?
show the reaction diagram
-, Q5YFS2
-
-
-
?
hexanamide + H2O
hexanoate + NH3
show the reaction diagram
-
-
-
-
?
hexanamide + H2O
hexanoate + NH3
show the reaction diagram
P95896
-
-
-
?
hexanamide + H2O
hexanoate + NH3
show the reaction diagram
-
-
-
-
?
hexanamide + H2O
hexanoate + NH3
show the reaction diagram
F8AEP0
38.7% of the activity with acetamide
-
-
?
hexanamide + hydroxylamine
hexanoylhydroxamic acid + NH3
show the reaction diagram
-
-
-
-
?
hexanoamide + H2O
hexanoic acid + NH3
show the reaction diagram
-
-
-
-
?
hydroxylamine + acylamide
acylhydroxamate + NH3
show the reaction diagram
-
acyltransferase activity
-
?
hydroxylamine hydrochloride + H2O
?
show the reaction diagram
-
-
-
-
?
hydroxylamine hydrochloride + H2O
?
show the reaction diagram
Q9EV64, -
-
-
-
?
hydroxylamine hydrochloride + H2O
?
show the reaction diagram
Rhodococcus erythropolis NCIMB11540
Q9EV64
-
-
-
?
ibuprofen amide + H2O
ibuprofen + NH3
show the reaction diagram
-
-
-
-
?
indole-3-acetamide + H2O
indole-3-acetic acid + NH3
show the reaction diagram
-
-
-
-
?
indole-3-acetamide + H2O
indole-3-acetic acid + NH3
show the reaction diagram
-
-
-
?
indole-3-acetamide + H2O
indole-3-acetic acid + NH3
show the reaction diagram
-
role of amidase 1 in auxin biosynthesis. Amidase 1 may be involved in de novo synthesis of indole-3-acetic acid in Arabidopsis thaliana
-
-
?
indole-3-acetamide + H2O
indole-3-acetate + NH3
show the reaction diagram
-
-
-
-
?
isobutyramide + H2O
isobutyrate + NH3
show the reaction diagram
-
-
-
?
isobutyramide + H2O
isobutyrate + NH3
show the reaction diagram
-
-
-
-
?
isobutyramide + H2O
isobutyrate + NH3
show the reaction diagram
-
substrate of the soluble enzyme and of the immobilized, cross-linked enzyme, preferred substrate
-
-
?
isobutyramide + H2O
isobutyrate + NH3
show the reaction diagram
Sulfolobus solfataricus, Sulfolobus solfataricus MT4
P95896
best aliphatic substrate
-
-
?
isobutyramide + H2O
isobutyrate + NH3
show the reaction diagram
P95896
-
-
-
?
isobutyramide + H2O
isobutyrate + NH3
show the reaction diagram
-
-
-
-
?
isobutyramide + H2O
isobutyric acid + NH3
show the reaction diagram
-
-
-
-
?
isobutyramide + H2O
isobutyric acid + NH3
show the reaction diagram
B4XEY3
-
-
-
?
isobutyramide + H2O
isobutanoate + NH3
show the reaction diagram
-
16% of the activity with acetamide
-
-
?
isobutyramide + hydroxylamine
isobutyrylhydroxamic acid + NH3
show the reaction diagram
-
-
-
-
?
isobutyramide + hydroxylamine
isobutyrylhydroxamic acid + NH3
show the reaction diagram
-
-
-
-
?
isonicotinamide + H2O
isonicotinic acid + NH3
show the reaction diagram
-
-
-
-
?
isonicotinamide + H2O
isonicotinic acid + NH3
show the reaction diagram
P95896
-
-
-
?
ketoprofen amide
2-(3-benzoylphenyl)propionic acid + NH3
show the reaction diagram
Rhodococcus erythropolis, Rhodococcus erythropolis MP50
-
-
-
?
L-alaninamide + H2O
L-Ala + NH3
show the reaction diagram
-
3% of the activity with acetamide
-
-
?
L-alaninamide + hydroxylamine
L-alaninoylhydroxamic acid + NH3
show the reaction diagram
-
-
-
-
?
L-alanineamide + H2O
L-alanine + NH3
show the reaction diagram
-
-
-
-
?
L-alanyl-gamma-D-glutamyl-meso-diaminopimelic acid + H2O
L-alanyl-gamma-D-glutamic acid + diaminopimelic acid
show the reaction diagram
-
murein tripeptide
-
-
?
L-asparagine + H2O
L-aspartate + NH3
show the reaction diagram
P95896
-
-
-
?
L-asparagine + H2O
L-aspartate + NH3
show the reaction diagram
F8AEP0
4.8% of the activity with acetamide
-
-
?
L-asparagine + H2O
L-aspartate + NH3
show the reaction diagram
-
4.8% of the activity with acetamide
-
-
?
L-asparagine + H2O
L-aspartate + NH3
show the reaction diagram
Sulfolobus solfataricus MT4
P95896
-
-
-
?
L-glutamine + H2O
L-glutamate + NH3
show the reaction diagram
P95896
-
-
-
?
L-glutamine + H2O
L-glutamate + NH3
show the reaction diagram
F8AEP0
73% of the activity with acetamide
-
-
?
L-glutamine + H2O
L-glutamate + NH3
show the reaction diagram
-
73% of the activity with acetamide
-
-
?
L-glutamine + H2O
L-glutamate + NH3
show the reaction diagram
Sulfolobus solfataricus MT4
P95896
-
-
-
?
L-leucinamide + hydroxylamine
L-leucinoylhydroxamic acid + NH3
show the reaction diagram
-
-
-
-
?
L-leucineamide + H2O
L-leucine + NH3
show the reaction diagram
-
-
-
-
?
L-methioninamide + hydroxylamine
L-methioninoylhydroxamic acid + NH3
show the reaction diagram
-
-
-
-
?
L-prolinamide + H2O
L-proline + NH3
show the reaction diagram
-
-
-
-
?
L-prolinamide + H2O
L-proline + NH3
show the reaction diagram
P95896
-
-
-
?
L-prolinamide + hydroxylamine
L-prolinoylhydroxamic acid + NH3
show the reaction diagram
-
-
-
-
?
L-threoninamide + hydroxylamine
L-threoninoylhydroxamic acid + NH3
show the reaction diagram
-
-
-
-
?
L-valineamide + H2O
L-valine + NH3
show the reaction diagram
-
-
-
-
?
lactamide + H2O
lactate + NH3
show the reaction diagram
P95896
-
-
-
?
malonamide + H2O
malonic acid + NH3
show the reaction diagram
-
-
-
-
?
mandelamide + H2O
mandelic acid + NH3
show the reaction diagram
Q9EV64, -
-
-
-
?
mandelamide + H2O
mandelic acid + NH3
show the reaction diagram
Rhodococcus erythropolis NCIMB11540
Q9EV64
-
-
-
?
metacrylamide + H2O
metacrylate + NH3
show the reaction diagram
-
-
-
-
?
metacrylamide + H2O
metacrylate + NH3
show the reaction diagram
-
-
-
-
-
methacrylamide + H2O
methacrylic acid + NH3
show the reaction diagram
-
-
-
-
?
methacrylamide + H2O
methacrylic acid + NH3
show the reaction diagram
-
17% of the activity with acetamide. The activity of the reverse reaction is 0.1% of the activity with acetic acid
-
-
?
methacrylamide + H2O
methacrylate + NH3
show the reaction diagram
P95896
-
-
-
?
mycothiol bimane + H2O
?
show the reaction diagram
-
-
-
-
?
N-(4-hydroxy-2-methylphenyl)arachidonylamide + H2O
4-amino-3-cresol + arachidonic acid
show the reaction diagram
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
-
i.e. VDM11, 15-20% of the activity with anandamide
-
-
?
N-(4-hydroxyphenyl)arachidonylamide + H2O
4-aminophenol + arachidonic acid
show the reaction diagram
-
i.e. AM404
-
-
?
n-butyramide + H2O
n-butyric acid + NH3
show the reaction diagram
-
75% of the activity with acetamide. The activity of the reverse reaction is 2% of the activity with acetic acid
-
-
r
N-lauroylethanolamine + H2O
lauric acid + ethanolamine
show the reaction diagram
-
-
-
-
?
N-methylacetamide + H2O
methylamine + acetate
show the reaction diagram
-
-
-
?
N-methylacetamide + H2O
acetic acid + methaneamine
show the reaction diagram
Pseudomonas aeruginosa, Pseudomonas aeruginosa PAC142
-
-
-
-
?
N-methylpropionamide + H2O
methylamine + propionate
show the reaction diagram
-
-
-
?
N-oleoyl ethanolamine + H2O
oleic acid + ethanolamine
show the reaction diagram
O00519, Q6GMR7
FAAH-2
-
-
?
N-oleoyl ethanolamine + H2O
oleic acid + NH3
show the reaction diagram
O00519, Q6GMR7
FAAH-1
-
-
?
N-oleoyl taurine + H2O
oleic acid + taurine
show the reaction diagram
O00519, Q6GMR7
FAAH-1
-
-
?
N-oleoylethanolamine + H2O
oleic acid + ethanolamine
show the reaction diagram
-, O08914, Q9D7V9
substrates in order of decreasing activity: anandamide, N-oleoylethanolamine, N-palmitoylethanolamine
-
-
?
N-oleoylethanolamine + H2O
oleic acid + ethanolamine
show the reaction diagram
P97612, Q5KTC7
substrates in order of decreasing activity: anandamide, N-oleoylethanolamine, N-palmitoylethanolamine
-
-
?
N-oleoylethanolamine + H2O
oleic acid + ethanolamine
show the reaction diagram
O00519, Q02083
substrates in order of decreasing activity: anandamide, N-oleoylethanolamine, N-palmitoylethanolamine
-
-
?
N-oleoylethanolamine + H2O
oleic acid + ethanolamine
show the reaction diagram
Q9TUI8
substrates in order of decreasing activity: anandamide, N-oleoylethanolamine, N-palmitoylethanolamine
-
-
?
N-oleoylethanolamine + H2O
oleic acid + ethanolamine
show the reaction diagram
O00519, Q02083
substrates in order of decreasing activity: N-palmitoylethanolamine, N-oleoylethanolamine, anandamide
-
-
?
N-oleoylethanolamine + H2O
oleic acid + ethanolamine
show the reaction diagram
P97612, Q5KTC7
substrates in order of decreasing activity: N-palmitoylethanolamine, N-oleoylethanolamine, anandamide
-
-
?
N-oleoylethanolamine + H2O
oleic acid + ethanolamine
show the reaction diagram
-, O08914, Q9D7V9
substrates in order of decreasing activity: N-palmitoylethanolamine, N-oleoylethanolamine, anandamide
-
-
?
N-palmitolyl ethanolamine + H2O
palmitic acid + ethanolamine
show the reaction diagram
O00519, Q6GMR7
FAAH-2
-
-
?
N-palmitolyl ethanolamine + H2O
palmitic acid + NH3
show the reaction diagram
O00519, Q6GMR7
FAAH-1
-
-
?
N-palmitoylethanolamine + H2O
palmitoic acid + ethanolamine
show the reaction diagram
-, O08914, Q9D7V9
substrates in order of decreasing activity: anandamide, N-oleoylethanolamine, N-palmitoylethanolamine
-
-
?
N-palmitoylethanolamine + H2O
palmitoic acid + ethanolamine
show the reaction diagram
P97612, Q5KTC7
substrates in order of decreasing activity: anandamide, N-oleoylethanolamine, N-palmitoylethanolamine
-
-
?
N-palmitoylethanolamine + H2O
palmitoic acid + ethanolamine
show the reaction diagram
O00519, Q02083
substrates in order of decreasing activity: anandamide, N-oleoylethanolamine, N-palmitoylethanolamine
-
-
?
N-palmitoylethanolamine + H2O
palmitoic acid + ethanolamine
show the reaction diagram
Q9TUI8
substrates in order of decreasing activity: anandamide, N-oleoylethanolamine, N-palmitoylethanolamine
-
-
?
N-palmitoylethanolamine + H2O
palmitoic acid + ethanolamine
show the reaction diagram
O00519, Q02083
substrates in order of decreasing activity: N-palmitoylethanolamine, N-oleoylethanolamine, anandamide
-
-
?
N-palmitoylethanolamine + H2O
palmitoic acid + ethanolamine
show the reaction diagram
P97612, Q5KTC7
substrates in order of decreasing activity: N-palmitoylethanolamine, N-oleoylethanolamine, anandamide
-
-
?
N-palmitoylethanolamine + H2O
palmitoic acid + ethanolamine
show the reaction diagram
-, O08914, Q9D7V9
substrates in order of decreasing activity: N-palmitoylethanolamine, N-oleoylethanolamine, anandamide
-
-
?
nicotinamide + H2O
nicotinic acid + NH3
show the reaction diagram
-
-
-
-
?
nicotinamide + H2O
nicotinic acid + NH3
show the reaction diagram
-
-
-
-
?
nicotinamide + H2O
nicotinic acid + NH3
show the reaction diagram
P95896
-
-
-
?
nicotinamide + H2O
nicotinic acid + NH3
show the reaction diagram
-
-
-
-
?
nicotinamide + H2O
nicotinic acid + NH3
show the reaction diagram
Mycobacterium avium, Torula cremoris
-
absolute specificity
-
-
?
nicotinamide + H2O
nicotinic acid + NH3
show the reaction diagram
Microbacterium imperiale CBS 498-74
-
-
-
-
?
nicotinamide + hydroxylamine
nicotinoylhydroxamic acid + NH3
show the reaction diagram
-
-
-
-
?
oleamide + H2O
oleic acid + NH3
show the reaction diagram
-
-
-
-
?
oleamide + H2O
oleic acid + NH3
show the reaction diagram
O00519, Q6GMR7
FAAH-1
-
-
?
oleamide + H2O
oleic acid + NH3
show the reaction diagram
O00519, Q6GMR7
FAAH-2
-
-
?
oleamide + H2O
?
show the reaction diagram
-
-
-
-
?
p-nitroacetanilide + H2O
p-nitroaniline + NH3
show the reaction diagram
P11436
-
-
-
?
p-nitroacetanilide + H2O
p-nitroaniline + acetate
show the reaction diagram
-
-
-
-
?
p-nitroacetanilide + H2O
acetic acid + p-nitroaniline
show the reaction diagram
-
mutant T103I
-
-
?
p-nitrophenylacetamide + H2O
p-nitrophenylacetic acid + NH3
show the reaction diagram
-
-
-
-
-
p-nitrophenylacetamide + H2O
p-nitrophenylacetic acid + NH3
show the reaction diagram
-
mutant W138G and T103I-W138G
-
-
?
pentanamide + H2O
pentanoate + NH3
show the reaction diagram
P95896
-
-
-
?
pentanamide + H2O
pentanoate + NH3
show the reaction diagram
-
-
-
-
?
pentanamide + H2O
pentanoate + NH3
show the reaction diagram
Sulfolobus solfataricus MT4
P95896
-
-
-
?
pentanamide + H2O
pentanoate + NH3
show the reaction diagram
P95896
-
-
-
?
phenetidine + acetate
phenacetin + H2O
show the reaction diagram
Delftia acidovorans, Delftia acidovorans AE1
-
transferase activity
-
?
phenylacetamide
phenylacetic acid + NH3
show the reaction diagram
-
-
-
-
-
phenylacetamide
phenylacetic acid + NH3
show the reaction diagram
-
-
-
?
phenylacetamide
phenylacetic acid + NH3
show the reaction diagram
Delftia acidovorans AE1
-
-
-
-
-
phenylacetamide
phenylacetic acid + NH3
show the reaction diagram
Rhodococcus erythropolis MP50
-
-
-
?
phenylacetamide + H2O
benzoic acid + NH3
show the reaction diagram
-
mutant W138G and T103I-W138G
-
-
?
picolinamide + H2O
picolinate + NH3
show the reaction diagram
P95896
-
-
-
?
pivalamide + H2O
pivalate + NH3
show the reaction diagram
Brevibacterium sp., Brevibacterium sp. R312
-
-
-
?
Pro-Phe-Arg benzylamide + H2O
Pro-Phe-Arg + benzylamine
show the reaction diagram
-
-
-
-
?
propanamide + H2O
propanoic acid + NH3
show the reaction diagram
Nesterenkonia sp., Nesterenkonia sp. AN1
C6K3Z5
-
-
-
?
propionamide + H2O
propionic acid + NH3
show the reaction diagram
-
-
-
-
?
propionamide + H2O
propionic acid + NH3
show the reaction diagram
-
-
-
?
propionamide + H2O
propionic acid + NH3
show the reaction diagram
-
-
-
-
?
propionamide + H2O
propionic acid + NH3
show the reaction diagram
-
-
-
-
?
propionamide + H2O
propionic acid + NH3
show the reaction diagram
-
79% of the activity with acetamide. The activity of the reverse reaction is 3% of the activity with acetic acid
-
-
r
propionamide + H2O
propionic acid + NH3
show the reaction diagram
Rhodococcus erythropolis, Rhodococcus erythropolis No. 7
B4XEY3
also acyl transfer activity in the presence of hydroxylamine
-
-
?
propionamide + H2O
propionic acid + NH3
show the reaction diagram
P95896
-
-
-
?
propionamide + H2O
propionic acid + NH3
show the reaction diagram
Pseudomonas chlororaphis B23
-
-
-
?
propionamide + H2O
propionic acid + NH3
show the reaction diagram
Rhodococcus sp. S13-4
-
79% of the activity with acetamide. The activity of the reverse reaction is 3% of the activity with acetic acid
-
-
r
propionamide + H2O
propionate + NH3
show the reaction diagram
-
-
-
-
?
propionamide + H2O
propionate + NH3
show the reaction diagram
-
-
-
-
?
propionamide + H2O
propionate + NH3
show the reaction diagram
Q7DKE4
-
-
-
?
propionamide + H2O
propionate + NH3
show the reaction diagram
P95896
-
-
-
?
propionamide + H2O
propionate + NH3
show the reaction diagram
-
-
-
-
?
propionamide + H2O
propionate + NH3
show the reaction diagram
-
67% of the activity with acetamide
-
-
?
propionamide + H2O
propionate + NH3
show the reaction diagram
-
substrate of the soluble enzyme and of the immobilized, cross-linked enzyme
-
-
?
propionamide + H2O
propionate + NH3
show the reaction diagram
-
-
-
-
?
propionamide + H2O
propanoate + NH3
show the reaction diagram
-
-
-
-
?
propionamide + hydroxylamine
propionylhydroxamic acid + NH3
show the reaction diagram
-
-
-
-
?
propionamide + hydroxylamine
propionylhydroxamic acid + NH3
show the reaction diagram
-
-
-
-
?
propionohydrazide + H2O
hydrazine + propionate
show the reaction diagram
-
-
-
?
propionohydroxamate + H2O
hydroxylamine + propionate
show the reaction diagram
-
-
-
?
pyrazinamide + H2O
pyrazinoic acid + NH3
show the reaction diagram
P95896
-
-
-
?
pyrazinamide + H2O
? + NH3
show the reaction diagram
-
-
-
-
?
RCONH2 + H2O
RCOOH + NH3
show the reaction diagram
-
-
-
?
RCONH2 + H2O
RCOOH + NH3
show the reaction diagram
-
-
-
?
RCONH2 + H2O
RCOOH + NH3
show the reaction diagram
Brevibacterium sp., Brevibacterium sp. R312
-
-
-
?
RCONH2 + H2O
RCOOH + NH3
show the reaction diagram
Escherichia coli JM109
-
-
-
?
RCONH2 + H2O
RCOOH + NH3
show the reaction diagram
Pseudomonas aeruginosa PAC 142 (L10R), Pseudomonas aeruginosa PAC 111 (C11)
-
-
-
?
RCONH2 + H2O
RCOOH + NH3
show the reaction diagram
Rhodococcus rhodochrous J1
-
-
-
?
salicylamide + H2O
salicylate + NH3
show the reaction diagram
P95896
-
-
-
?
short-chain amides + hydrazine
acid hydrazides + NH3
show the reaction diagram
-
-
-
-
?
succinamic acid + H2O
succinic acid + NH3
show the reaction diagram
Blastobacter sp.
-
-
-
?
succinamide + H2O
succinic acid + NH3
show the reaction diagram
-
-
-
-
?
trimethylacetamide + H2O
trimethylacetic acid + NH3
show the reaction diagram
-
-
-
-
?
urea + H2O
?
show the reaction diagram
-
-
-
-
?
urea + H2O
?
show the reaction diagram
Aeribacillus pallidus, Aeribacillus pallidus RAPc8
-
1.5% of the activity with acetamide
-
-
?
urea + H2O
carbamic acid + NH3
show the reaction diagram
-
-
-
-
?
urea + H2O
formamide + NH3
show the reaction diagram
-
-
-
-
?
valeramide + H2O
valerate + NH3
show the reaction diagram
Brevibacterium sp., Brevibacterium sp. R312
-
-
-
?
monoamidated dicarboxylate + H2O
dicarboxylate + ammonia
show the reaction diagram
Blastobacter sp.
-
-
-
?
additional information
?
-
-
involved in the hydrolysis of the cell wall
-
-
-
additional information
?
-
-
amidation of the C-terminal carboxylic group of several N-protected di-, tri-, tetra and pentapeptides
-
-
?
additional information
?
-
-
other fatty acid ethanolamides may act as substrate
-
-
-
additional information
?
-
-
the enzyme is specific for cleavage of the gamma-D-glutamyl-meso-diaminopimelic acid bond of free murein tripeptide but not of peptides linked to muramic acid
-
-
-
additional information
?
-
-, Q5YFS2
substrate specificity towards cephalosporin analogues, overview, no activity with cephalosporin C
-
-
-
additional information
?
-
-
substrate synthesis, overview, substrate specificity of the stereospecific enzyme, no activity with 2-hydroxy-2-(trifluoromethyl)-pentanamide, 3,3,3-trifluoro-2-hydroxy-2-phenyl-propionamide, 3,3,3-trichloro-2-hydroxy-2-methylpropionamide, and 3,3,3-trifluoro-2-methoxy-2-methylpropionamide, overview
-
-
-
additional information
?
-
Q9EV64, -
the enzyme is absolutely enantioselective, biocatalytic hydrolysis of cyanohydrins of amidase with nitrile hydratase, overview
-
-
-
additional information
?
-
-
the enzyme is enantioselective, the enzyme also shows acyl transferase and hydrolase activities, no activity with N-methylurea, fumaramide, 4-aminobenzosulfonamide, N-phenylurea, acetanilide, benzylcarbamate, and o-tolylamide, broad substrate spectrum, including aliphatic, aromatic and amino acid amides, the presence of a double bond or a methyl group near the carboxamide group of aliphatic and amino acid amides enhances the enzymatic activity, among aromatic amides with substitutions at the 2-, 3-, or 4-position, the 4-substituted amides are the preferred substrates, detailed overview
-
-
-
additional information
?
-
-
the Fv dimer was constructed by using the crystallographic structure of antibody 25.3, PDB 1AFV, as a template, activity of the amide hydrolyzing antibody 312D6 with sulfonamide hapten substrates, complex formation and structure, antibody 312d6 is highly specific for p-tolylsubstituted indole sulfonamides and carboxamides, docking analysis, overview
-
-
-
additional information
?
-
-
the human tissue kallikrein shows amidase activity, the enzyme hydrolyzes various synthetic substrates such as Nalpha-substituted arginine and lysine derivatives as esters, amides and fluorogenic peptides, overview
-
-
-
additional information
?
-
-
stereoselective enzyme
-
-
-
additional information
?
-
-
the enzyme catalyses the hydrolysis of small aliphatic amides
-
-
-
additional information
?
-
-
AtFAAH is one, but not the only, modulator of endogenous N-acylethanolamine levels in plants (N-acylethanolamine depletion likely participates in the regulation of plant growth)
-
-
-
additional information
?
-
-
FAAH is a key regulator in the development of ethanol dependence
-
-
-
additional information
?
-
O00519, Q6GMR7
FAAH-1 plays a primary role in regulating endocannabinoid signaling
-
-
-
additional information
?
-
-
acyl transfer reactions are demonstrated with acetamide, propionamide, isobutyramide, and acrylamide as substrates and hydroxylamine as the acyl acceptor. The highest reaction rate being with isobutyramide. Immobilization by entrapment in polyacrylamide gels, covalent binding on Eupergit C beads at 4C and on Amberlite-XAD57 results in low protein binding and low activity, but immobilization on Eupergit C beads at 25C with cross-linking resulted in high protein binding yield and high immobilized specific activity. No activity with: nicotinamide, isonicotinamide, L-asparagine, D-asparagine, DL-phenylalanine, L-prolinamide, hexanamide
-
-
-
additional information
?
-
O00519, Q02083
hardly hydrolyzes 2-arachidonoylglycerol
-
-
-
additional information
?
-
P97612, Q5KTC7
hardly hydrolyzes 2-arachidonoylglycerol
-
-
-
additional information
?
-
-, O08914, Q9D7V9
hardly hydrolyzes 2-arachidonoylglycerol
-
-
-
additional information
?
-
-
N-acylethanolamines and oleamide are not hydrolyzed signifiantly
-
-
-
additional information
?
-
-
no activity with nicotinamide or benzamide
-
-
-
additional information
?
-
O00519, Q6GMR7
no FAAH-2 activity is detected with N-oleoyl taurine
-
-
-
additional information
?
-
-
not active on L-lactamide
-
-
-
additional information
?
-
-
the amidase shows high enantioselectivity for (S)-arylpropionic amides, it is active on nitriles, molecular modelling, overview
-
-
-
additional information
?
-
B4XEY3
enantioselectivity toward (R)-4-chloro-3-hydroxybutyramide: e.e value 52% (conversion yield 57%)
-
-
-
additional information
?
-
Q7DKE4
the Ser-cis Ser-Lys catalytic triad is located in the large domain. But the substrate-binding pocket is relatively narrow, due to the presence of the helix alpha13 in the small domain. The hydrophobic residues from the small domain are involved in recognizing the substrate. The small domain likely participates in substrate recognition and is related to the difference of substrate specificities among the AS family amidases
-
-
-
additional information
?
-
-
formation of acetohydroxamic acid using acetamide and hydroxylamine as substrates
-
-
-
additional information
?
-
-
no activity with methacrylamide and nicotinamide
-
-
-
additional information
?
-
Nesterenkonia sp.
C6K3Z5
the enzyme NitN has an active site and a fold characteristic of the nitrilase superfamily, but shows only amidase activity, no nitrilase activity
-
-
-
additional information
?
-
P95896
the enzyme transfers the acyl group also to hydroxylamine and hydrazine
-
-
-
additional information
?
-
F8AEP0
no activity with: urea, benzamide, pyrazinamide, nicotinamide, cinnamamide, salicylamide
-
-
-
additional information
?
-
Aeribacillus pallidus RAPc8
-
acyl transfer reactions are demonstrated with acetamide, propionamide, isobutyramide, and acrylamide as substrates and hydroxylamine as the acyl acceptor. The highest reaction rate being with isobutyramide. Immobilization by entrapment in polyacrylamide gels, covalent binding on Eupergit C beads at 4C and on Amberlite-XAD57 results in low protein binding and low activity, but immobilization on Eupergit C beads at 25C with cross-linking resulted in high protein binding yield and high immobilized specific activity. No activity with: nicotinamide, isonicotinamide, L-asparagine, D-asparagine, DL-phenylalanine, L-prolinamide, hexanamide, not active on L-lactamide
-
-
-
additional information
?
-
F8AEP0, -
no activity with: urea, benzamide, pyrazinamide, nicotinamide, cinnamamide, salicylamide
-
-
-
additional information
?
-
Rhodococcus erythropolis No. 7
B4XEY3
enantioselectivity toward (R)-4-chloro-3-hydroxybutyramide: e.e value 52% (conversion yield 57%)
-
-
-
additional information
?
-
Sulfolobus solfataricus MT4
P95896
the enzyme transfers the acyl group also to hydroxylamine and hydrazine
-
-
-
additional information
?
-
Pseudomonas aeruginosa 8602
-
formation of acetohydroxamic acid using acetamide and hydroxylamine as substrates
-
-
-
additional information
?
-
C6K3Z5
the enzyme NitN has an active site and a fold characteristic of the nitrilase superfamily, but shows only amidase activity, no nitrilase activity
-
-
-
additional information
?
-
Pseudomonas putida 5B
-
stereoselective enzyme
-
-
-
additional information
?
-
Rhodococcus erythropolis NCIMB11540
Q9EV64
the enzyme is absolutely enantioselective, biocatalytic hydrolysis of cyanohydrins of amidase with nitrile hydratase, overview
-
-
-
additional information
?
-
-
no activity with methacrylamide and nicotinamide
-
-
-
additional information
?
-
Rhodococcus sp. S13-4
-
no activity with nicotinamide or benzamide
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(R)-(+)-2-chloropropionamide + H2O
(+)-2-chloropropionic acid + NH3
show the reaction diagram
Escherichia coli, Escherichia coli JM109
-
-
-
?
(S)-(-)-2-chloropropionamide + H2O
(-)-2-chloropropionic acid + NH3
show the reaction diagram
Escherichia coli, Escherichia coli JM109
-
-
-
?
1-naphthaleneacetamide + H2O
1-naphthaleneacetic acid + NH3
show the reaction diagram
-
-
-
?
2 indole-3-acetonitrile + 3 H2O
indole-3-acetamide + indole-3-acetic acid + NH3
show the reaction diagram
-
-
-
?
2-arylpropionic amide + H2O
S-2-arylpropionic acid + NH3
show the reaction diagram
Delftia acidovorans, Delftia acidovorans KPO-2771-4
-
enantioselective S-amidase
-
?
2-chloropropionamide + H2O
2-chloropropionate + NH3
show the reaction diagram
Pseudomonas putida, Pseudomonas putida PP3
-
-
-
?
2-nitroacetanilide
2-nitrophenylamine + acetate
show the reaction diagram
Delftia acidovorans, Delftia acidovorans AE1
-
-
-
?
3,3,3-trifluoro-2-hydroxy-2-methylpropanamide + H2O
3,3,3-trifluoro-2-hydroxy-2-methylpropanoic acid + NH3
show the reaction diagram
-
-
-
-
?
3-hydroxymyristoyl lipid A + H2O
?
show the reaction diagram
-
3-hydroxymyristoyl group linked to the proximal glucosamine residue of de-O-acylated lipid A, hydolysis by isozyme FAAI
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
show the reaction diagram
Delftia acidovorans, Delftia acidovorans AE1
-
-
-
?
acetamide + H2O
acetic acid + NH3
show the reaction diagram
-
-
-
?
acetanilide + H2O
acetate + aniline
show the reaction diagram
Pseudomonas aeruginosa, Pseudomonas aeruginosa AI3
-
-
-
?
acetohydrazide + H2O
hydrazine + acetate
show the reaction diagram
-
-
-
?
acetohydroxamate + H2O
hydroxylamine + acetate
show the reaction diagram
-
-
-
?
acrylamide + H2O
acrylic acid + NH3
show the reaction diagram
-
-
-
?
acylamide + H2O
carboxylic acid + NH3
show the reaction diagram
-
-
-
?
anandamide + H2O
?
show the reaction diagram
-
-
-
-
?
anandamide + H2O
?
show the reaction diagram
-
i.e. arachidonoylethanolamide
-
-
?
anandamide + H2O
arachidonic acid + ethanolamine
show the reaction diagram
-
-
-
-
?
anandamide + H2O
?
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
i.e. arachidonoylethanolamide
-
-
?
benzamide
benzoic acid + NH3
show the reaction diagram
Blastobacter sp.
-
-
-
?
benzamide + H2O
benzoic acid + NH3
show the reaction diagram
Escherichia coli, Escherichia coli JM109
-
-
-
?
butyramide + H2O
n-butyrate + NH3
show the reaction diagram
-
-
-
?
carboxylic acid + hydroxylamine
N-hydroxy-carboxylic acid amide + H2O
show the reaction diagram
Brevibacterium sp., Brevibacterium sp. R312
-
acid transferase transferase activity
-
?
carboxylic acid amide + H2O
carboxylic acid + NH3
show the reaction diagram
Brevibacterium sp., Brevibacterium sp. R312
-
-
-
?
carboxylic acid amide + hydroxylamine
N-hydroxy-carboxylic acid amide + NH3
show the reaction diagram
Brevibacterium sp., Brevibacterium sp. R312
-
amide transferase activity
-
?
carboxylic acid ester + hydroxylamine
N-hydroxy-carboxylic acid amide + alcohol
show the reaction diagram
Brevibacterium sp., Brevibacterium sp. R312
-
ester transferase activity
-
?
DL-lactamide + H2O
DL-lactate + NH3
show the reaction diagram
Brevibacterium sp., Brevibacterium sp. R312
-
-
-
?
ethyl acetate + H2O
ethanol + acetate
show the reaction diagram
-
-
-
?
ethyl acrylate + H2O
acrylate + ethanol
show the reaction diagram
-
-
-
?
ethyl propionate + H2O
ethanol + propionate
show the reaction diagram
-
-
-
?
glutaramic acid + H2O
glutaric acid + NH3
show the reaction diagram
Blastobacter sp.
-
-
-
?
hydroxylamine + acylamide
acylhydroxamate + NH3
show the reaction diagram
-
acyltransferase activity
-
?
indole-3-acetamide + H2O
indole-3-acetic acid + NH3
show the reaction diagram
-
-
-
?
indole-3-acetamide + H2O
indole-3-acetic acid + NH3
show the reaction diagram
-
role of amidase 1 in auxin biosynthesis. Amidase 1 may be involved in de novo synthesis of indole-3-acetic acid in Arabidopsis thaliana
-
-
?
isobutyramide + H2O
isobutyrate + NH3
show the reaction diagram
-
-
-
?
ketoprofen amide
2-(3-benzoylphenyl)propionic acid + NH3
show the reaction diagram
Rhodococcus erythropolis, Rhodococcus erythropolis MP50
-
-
-
?
N-methylacetamide + H2O
methylamine + acetate
show the reaction diagram
-
-
-
?
N-methylpropionamide + H2O
methylamine + propionate
show the reaction diagram
-
-
-
?
phenetidine + acetate
phenacetin + H2O
show the reaction diagram
Delftia acidovorans, Delftia acidovorans AE1
-
transferase activity
-
?
phenylacetamide
phenylacetic acid + NH3
show the reaction diagram
Rhodococcus erythropolis, Rhodococcus erythropolis MP50
-
-
-
?
pivalamide + H2O
pivalate + NH3
show the reaction diagram
Brevibacterium sp., Brevibacterium sp. R312
-
-
-
?
propionamide + H2O
propionic acid + NH3
show the reaction diagram
Pseudomonas chlororaphis, Pseudomonas chlororaphis B23
-
-
-
?
propionohydrazide + H2O
hydrazine + propionate
show the reaction diagram
-
-
-
?
propionohydroxamate + H2O
hydroxylamine + propionate
show the reaction diagram
-
-
-
?
RCONH2 + H2O
RCOOH + NH3
show the reaction diagram
-
-
-
?
RCONH2 + H2O
RCOOH + NH3
show the reaction diagram
-
-
-
?
RCONH2 + H2O
RCOOH + NH3
show the reaction diagram
Brevibacterium sp., Brevibacterium sp. R312
-
-
-
?
RCONH2 + H2O
RCOOH + NH3
show the reaction diagram
Escherichia coli JM109
-
-
-
?
RCONH2 + H2O
RCOOH + NH3
show the reaction diagram
Pseudomonas aeruginosa PAC 142 (L10R), Pseudomonas aeruginosa PAC 111 (C11)
-
-
-
?
RCONH2 + H2O
RCOOH + NH3
show the reaction diagram
Rhodococcus rhodochrous J1
-
-
-
?
succinamic acid + H2O
succinic acid + NH3
show the reaction diagram
Blastobacter sp.
-
-
-
?
valeramide + H2O
valerate + NH3
show the reaction diagram
Brevibacterium sp., Brevibacterium sp. R312
-
-
-
?
monoamidated dicarboxylate + H2O
dicarboxylate + ammonia
show the reaction diagram
Blastobacter sp.
-
-
-
?
additional information
?
-
-
involved in the hydrolysis of the cell wall
-
-
-
additional information
?
-
-
AtFAAH is one, but not the only, modulator of endogenous N-acylethanolamine levels in plants (N-acylethanolamine depletion likely participates in the regulation of plant growth)
-
-
-
additional information
?
-
-
FAAH is a key regulator in the development of ethanol dependence
-
-
-
additional information
?
-
O00519, Q6GMR7
FAAH-1 plays a primary role in regulating endocannabinoid signaling
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ba2+
-
activity enhanced
Ca2+
Q70I53
activation above 100 mM
Co2+
Q70I53
increases 1.3fold the activity at 1 mM, inhibition at higher concentration
Co2+
-
1 mM, about 20% activation
Cobalt
-
0.33 mol of cobalt per mol of the native enzyme, coupled plasma-atomic emission spectrometry analysis
Cr2+
-
activity enhanced
Fe3+
-
1 mM, about 20% activation
Iron
-
8 mol of iron per mol of enzyme, detection by inductively coupled plasma-atomic emission spectrometry
Iron
-
0.33 mol of iron per mol of the native enzyme, coupled plasma-atomic emission spectrometry analysis
Mg2+
Q70I53
activation above 100 mM
Mg2+
-
required for enzyme activity
Mg2+
F8AEP0
10 mM, 1.15fold stimulation
NaCl
-
influences the oligomerization status of the enzyme. 60% octameric and 40% dimeric enzyme in absence of NaCl, 5% octameric and 95% dimeric enzyme at and above 150 mM NaCl, profile overview
Ni2+
Q70I53
increases 1.1fold the activity at 1 mM, inhibition at higher concentration
V2+
-
1 mM, about 20% activation
Zn2+
Q70I53
required for activity
Mn2+
Q70I53
activation above 100 mM
additional information
-
enzyme has no metal ion requirement
additional information
-
no effect: Cu2+, Mn2+, and Ca2+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
(+/-)-(5Z,8Z,11Z,14Z)-N-((2,2-dimethyl-1,3-dioxolan-4-yl)methyl)icosa-5,8,11,14-tetraenamide
-
-
(+/-)-2,2-dimethyl-1,3-dioxolan-4-ylmethyl (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate
-
-
(+/-)-oxiran-2-ylmethyl (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate
-
-
(+/-)-oxiran-2-ylmethyl (9Z)-hexadec-9-enoate
-
-
(+/-)-oxiran-2-ylmethyl (9Z)-octadec-9-enoate
-
-
(+/-)-oxiran-2-ylmethyl (9Z,12Z)-octadeca-9,12-dienoate
-
-
(+/-)-oxiran-2-ylmethyl (9Z,12Z,15Z)-octadeca-9,12,15-trienoate
-
-
(+/-)-oxiran-2-ylmethyl 1,1'-biphenyl-2-carboxylate
-
-
(+/-)-oxiran-2-ylmethyl 1,1'-biphenyl-3-carboxylate
-
-
(+/-)-oxiran-2-ylmethyl 1,1'-biphenyl-4-carboxylate
-
-
(+/-)-oxiran-2-ylmethyl benzoate
-
-
(+/-)-tetrahydro-2H-pyran-2-ylmethyl (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate
-
-
(+/-)-tetrahydro-2H-pyran-2-ylmethyl (9Z)-hexadec-9-enoate
-
-
(+/-)-tetrahydro-2H-pyran-2-ylmethyl (9Z)-octadec-9-enoate
-
-
(+/-)-tetrahydro-2H-pyran-2-ylmethyl (9Z,12Z)-octadeca-9,12-dienoate
-
-
(+/-)-tetrahydro-2H-pyran-2-ylmethyl (9Z,12Z,15Z)-octadeca-9,12,15-trienoate
-
-
(+/-)-tetrahydro-2H-pyran-2-ylmethyl 1,1'-biphenyl-2-carboxylate
-
-
(+/-)-tetrahydro-2H-pyran-2-ylmethyl 1,1'-biphenyl-3-carboxylate
-
-
(+/-)-tetrahydro-2H-pyran-2-ylmethyl 1,1'-biphenyl-4-carboxylate
-
-
(+/-)-tetrahydro-2H-pyran-2-ylmethyl benzoate
-
-
(2R,3R)-allo-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester
-
-
(2R,3R)-allo-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester
-
-
(2R,3S)-2-methyl-4-oxo-3-oxetanylcarbamic acid benzyl ester
-
-
(2R,3S)-2-methyl-4-oxo-3-oxetanylcarbamic acid benzyl ester
-
-
(2R,3S)-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester
-
-
(2R,3S)-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester
-
-
(2S,3R)-2-methyl-4-oxo-3-oxetanylcarbamic acid 5-phenylpentyl ester
-
potent inhibitor, rapidly cleaved in plasma
(2S,3R)-2-methyl-4-oxo-3-oxetanylcarbamic acid 5-phenylpentyl ester
-
potent inhibitor, rapidly cleaved in plasma
(2S,3R)-2-methyl-4-oxo-3-oxetanylcarbamic acid benzyl ester
-
-
(2S,3R)-2-methyl-4-oxo-3-oxetanylcarbamic acid benzyl ester
-
-
(2S,3R)-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester
-
-
(2S,3R)-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester
-
-
(2S,3S)-allo-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester
-
-
(2S,3S)-allo-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester
-
-
(5Z,8Z,11Z,14Z)-eicosantetraenoic acid 3-thienyl methyl ester
-
i.e. CAY-10402, IC50: 0.010 mM, 37% inhibition at 0.1 mM
(5Z,8Z,11Z,14Z)-eicosantetraenoic acid 3-thienyl methyl ester
-
i.e. CAY-10402
(R)-(2-oxo-3-oxetanyl)carbamic acid tert-butyl ester
-
-
(R)-(2-oxo-3-oxetanyl)carbamic acid tert-butyl ester
-
-
(R)-1-methyl-3-(2-oxo-3-oxetanyl)urea
-
-
(R)-1-methyl-3-(2-oxo-3-oxetanyl)urea
-
-
(R)-2-oxo-3-oxetanylcarbamic acid benzyl ester
-
-
(R)-2-oxo-3-oxetanylcarbamic acid benzyl ester
-
-
(R,S)-2-oxocyclobutylcarbamic acid benzyl ester
-
weak inhibition
(R,S)-2-oxocyclobutylcarbamic acid benzyl ester
-
weak inhibition
(S)-(2-oxo-3-oxetanyl)carbamic acid tert-butyl ester
-
-
(S)-(2-oxo-3-oxetanyl)carbamic acid tert-butyl ester
-
-
(S)-1-methyl-3-(2-oxo-3-oxetanyl)urea
-
-
(S)-1-methyl-3-(2-oxo-3-oxetanyl)urea
-
-
(S)-2-oxo-3-oxetanylcarbamic acid benzyl ester
-
-
(S)-2-oxo-3-oxetanylcarbamic acid benzyl ester
-
-
1,3-benzodioxol-5-ylmethyl (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate
-
-
1-(1,3-oxazol-2-yl)-3-(4-phenoxyphenyl)propan-1-one
-
-
1-(1,3-oxazol-2-yl)-3-[4-(phenoxymethyl)phenyl]propan-1-one
-
-
1-(1,3-oxazol-2-yl)-4-piperidin-4-ylbutan-1-one
-
-
1-(1,3-oxazol-2-yl)-4-[1-(3-phenylpropanoyl)piperidin-4-yl]butan-1-one
-
-
1-(1,3-oxazol-2-yl)-4-[1-(phenylacetyl)piperidin-4-yl]butan-1-one
-
-
1-(1,3-oxazol-2-yl)-4-[1-(phenylsulfonyl)piperidin-4-yl]butan-1-one
-
-
1-(1,3-oxazol-2-yl)-4-[1-[(2-phenylethyl)sulfonyl]piperidin-4-yl]butan-1-one
-
-
1-(1,3-oxazol-2-yl)-7-phenylheptan-1-one
-
-
1-(5-iodo-1,3-oxazol-2-yl)-3-(4-phenoxyphenyl)propan-1-one
-
-
1-(5-iodo-1,3-oxazol-2-yl)-3-[4-(phenoxymethyl)phenyl]propan-1-one
-
-
1-(5-iodo-1,3-oxazol-2-yl)-7-phenylheptan-1-one
-
-
1-(5-pyridin-2-yl-1,3-oxazol-2-yl)-7-(2-thienyl)heptan-1-one
-
-
1-(5-pyridin-2-yl-1,3-oxazol-2-yl)-7-(3-thienyl)heptan-1-one
-
-
1-(5-pyridin-2-yl-1,3-oxazol-2-yl)-7-[2-(trifluoromethyl)phenyl]hept-6-yn-1-one
-
-
1-(5-pyridin-2-yl-1,3-oxazol-2-yl)-7-[2-(trifluoromethyl)phenyl]heptan-1-one
-
-
1-(5-pyridin-2-yl-1,3-oxazol-2-yl)-7-[3-(trifluoromethyl)phenyl]hept-6-yn-1-one
-
-
1-(5-pyridin-2-yl-1,3-oxazol-2-yl)-7-[3-(trifluoromethyl)phenyl]heptan-1-one
-
-
1-(5-pyridin-2-yl-1,3-oxazol-2-yl)-7-[4-(trifluoromethyl)phenyl]hept-6-yn-1-one
-
-
1-(5-pyridin-2-yl-1,3-oxazol-2-yl)-7-[4-(trifluoromethyl)phenyl]heptan-1-one
-
-
1-([4'-[3-(1,3-oxazol-2-yl)propyl]biphenyl-4-yl]methyl)piperidine
-
-
1-arachidin
-
10% inhibition at 0.1 mM
1-arachidonoylglycerol
-
IC50: 0.0062 mM
1-arachidonoylglycerol
-
42% inhibition at 0.1 mM
1-myristin
-
IC50: 0.018, complete inhibition is possible
1-nor-arachidonoyl-3-(2'3-dihydroxypropyl) urea
-
i.e. O-1502, complete inhibition at 0.1 mM
1-palmitin
-
IC50: 0.0080, complete inhibition is possible
1-[6-(2-methyl-4,5-diphenyl-1H-imidazol-1-yl)hexyl]-3-phenylurea
-
-
11-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)undecan-1-one
-
-
2,3-dihydroxypropyl (11Z)-icos-11-enoate
-
i.e. O-4066, IC50: 0.010, complete inhibition is possible
2,3-dihydroxypropyl (11Z,14Z)-icosa-11,14-dienoate
-
i.e. O-3907, IC50: 0.0057, complete inhibition is possible
2,3-dihydroxypropyl (4Z,7Z,10Z,13Z)-17-ethylcycloheptadeca-4,7,10,13-tetraene-1-carboxylate
-
i.e. O-1428, 28% inhibition at 0.1 mM
2,3-dihydroxypropyl (5Z)-icos-5-enoate
-
i.e. 3908, IC50: 0.011, 84% inhibition is possible
2,3-dihydroxypropyl (5Z,8Z,11Z)-2-ethylcycloheptadeca-5,8,11-triene-1-carboxylate
-
i.e. O-3973, IC50: 0.0024, 95% inhibition is possible
2,3-dihydroxypropyl (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoate
-
i.e. O-3832, IC50: 0.0076, complete inhibition is possible
2,3-dihydroxypropyl (6Z,9Z,12Z,15Z)-cyclohenicosa-6,9,12,15-tetraene-1-carboxylate
-
i.e. O-4081, IC50: 0.0051, 90% inhibition is possible
2,3-dihydroxypropyl (7Z,10Z,13Z,16Z)-docosa-7,10,13,16-tetraenoate
-
i.e. O-3872, IC50: 0.014, complete inhibition is possible
2,3-dihydroxypropyl (8Z,11Z,14Z)-icosa-8,11,14-trienoate
-
i.e. 3846, IC50: 0.023, complete inhibition is possible
2,6-difluorophenyl [6-[4,5-bis(4-fluorophenyl)-2-methyl-1H-imidazol-1-yl]hexyl]carbamate
-
-
2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazole-5-carbonitrile
-
-
2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazole-5-carboxamide
-
-
2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazole-5-carboxylic acid
-
-
2-(3-biphenyl-4-ylpropanoyl)-N,N-dimethyl-1,3-oxazole-5-carboxamide
-
-
2-(4-phenylbutoxy)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)ethanone
-
-
2-(5,5-dimethyl-1,3-dioxan-2-yl)ethyl (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate
-
-
2-(7-phenylheptanoyl)-1,3-oxazole-5-carbonitrile
-
-
2-(7-phenylheptanoyl)-1,3-oxazole-5-carboxamide
-
-
2-(7-phenylheptanoyl)-1,3-oxazole-5-carboxylic acid
-
-
2-arachidonoylglycerol
-
37% inhibition at 0.1 mM
2-chloro-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)ethanone
-
-
2-fluorophenyl [1-ethyl-6-(2-methyl-4,5-diphenyl-1H-imidazol-1-yl)hexyl]carbamate
-
-
2-fluorophenyl [1-methyl-6-(2-methyl-4,5-diphenyl-1H-imidazol-1-yl)hexyl]carbamate
-
-
2-fluorophenyl [6-(2-methyl-4,5-diphenyl-1H-imidazol-1-yl)-1-(1-methylethyl)hexyl]carbamate
-
-
2-fluorophenyl [6-(2-methyl-4,5-diphenyl-1H-imidazol-1-yl)-1-phenylhexyl]carbamate
-
-
2-fluorophenyl [6-(2-methyl-4,5-diphenyl-1H-imidazol-1-yl)hexyl]carbamate
-
-
2-fluorophenyl [6-(2-methyl-4,5-diphenyl-1H-imidazol-1-yl)hexyl]carbamate
-
potentiates the effects of exogenous anandamide (1 mg/kg, iv) in a rat thermal escape test (Hargreaves test), and shows robust antinociceptive activity in (1 mg/kg, iv) in a rat thermal escape test (Hargreaves test), and shows robust antinociceptive activity in animal models of persistent (formalin test) and neuropathic (Chung model) pain
2-fluorophenyl [6-[4,5-bis(4-fluorophenyl)-2-methyl-1H-imidazol-1-yl]hexyl]carbamate
-
-
2-hydroxy-7-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
2-mercaptoethanol
-
complete inhibition at 5% (v/v) 2-mercaptoethanol
2-[(4-phenylbutyl)sulfinyl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)ethanone
-
-
2-[(4-phenylbutyl)sulfonyl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)ethanone
-
-
2-[(4-phenylbutyl)thio]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)ethanone
-
-
2-[3-(4-phenoxyphenyl)propanoyl]-1,3-oxazole-5-carbonitrile
-
-
2-[3-(4-phenoxyphenyl)propanoyl]-1,3-oxazole-5-carboxamide
-
-
2-[3-(4-phenoxyphenyl)propanoyl]-1,3-oxazole-5-carboxylic acid
-
-
2-[3-(benzyloxy)phenoxy]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)ethanone
-
-
2-[3-[4-(phenoxymethyl)phenyl]propanoyl]-1,3-oxazole-5-carbonitrile
-
-
2-[3-[4-(phenoxymethyl)phenyl]propanoyl]-1,3-oxazole-5-carboxamide
-
-
2-[3-[4-(phenoxymethyl)phenyl]propanoyl]-1,3-oxazole-5-carboxylic acid
-
-
2-[4-(benzyloxy)phenoxy]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)ethanone
-
-
2-[methyl(4-phenylbutyl)amino]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)ethanol
-
-
3'-(aminocarbonyl)biphenyl-3-yl cyclohexylcarbamate
O00519, Q6GMR7
;
3'-carbamoyl-biphenyl-3-yl-cyclohexylcarbamate
-
i.e. URB597, specific, complete inhibition at 0.001 mM
3'-carbamoyl-biphenyl-3-yl-cyclohexylcarbamate
-
i.e. URB597, specific
3'-carbamoylbiphenyl-3-yl undec-10-yn-1-ylcarbamate
O00519, Q6GMR7
;
3'-{[(E)-(cyclohexylimino)(hydroxy)methyl]oxy}biphenyl-3-carboximidic acid
-
the inhibitor markedly increases the preference for ethanol. The inhibitor specifically acts through FAAH
3-(2-naphthyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
3-(3-phenylpropoxy)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
3-(4-anilinophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
3-(4-benzylphenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
3-(4-phenoxyphenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
3-(4-phenoxyphenyl)-1-[5-(trifluoromethyl)-1,3-oxazol-2-yl]propan-1-one
-
-
3-(4-phenylbutyl)oxetan-2-one
-
-
3-(4-phenylbutyl)oxetan-2-one
-
-
3-biphenyl-4-yl-1-(1,3-oxazol-2-yl)propan-1-one
-
-
3-biphenyl-4-yl-1-(5-iodo-1,3-oxazol-2-yl)propan-1-one
-
-
3-biphenyl-4-yl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
3-biphenyl-4-yl-1-(5-pyridin-3-yl-1,3-oxazol-2-yl)propan-1-one
-
-
3-biphenyl-4-yl-1-(5-pyridin-4-yl-1,3-oxazol-2-yl)propan-1-one
-
-
3-biphenyl-4-yl-1-[5-(methylthio)-1,3-oxazol-2-yl]propan-1-one
-
-
3-biphenyl-4-yl-1-[5-(trifluoroacetyl)-1,3-oxazol-2-yl]propan-1-one
-
-
3-biphenyl-4-yl-1-[5-(trifluoromethyl)-1,3-oxazol-2-yl]propan-1-one
-
-
3-biphenyl-4-yl-1-[5-[6-(1H-tetrazol-1-yl)pyridin-2-yl]-1,3-oxazol-2-yl]propan-1-one
-
-
3-hydroxy-7-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
3-[3-(benzyloxy)phenyl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
3-[4-(benzyloxy)cyclohexa-1,5-dien-1-yl]-1-[1,3]oxazolo[4,5-b]pyridin-2-ylpropan-1-one
-
-
3-[4-(benzyloxy)phenyl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
3-[4-(benzyloxy)phenyl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
3-[4-(phenoxymethyl)phenyl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
3-[4-(phenoxymethyl)phenyl]-1-[5-(trifluoromethyl)-1,3-oxazol-2-yl]propan-1-one
-
-
3-[4-(phenoxymethyl)phenyl]-1-[5-[6-(1H-tetrazol-5-yl)pyridin-2-yl]-1,3-oxazol-2-yl]propan-1-one
-
-
3-[4-(phenylthio)phenyl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
3-[4-[hydroxy(phenyl)methyl]cyclohexa-1,5-dien-1-yl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
3-[4-[hydroxy(phenyl)methyl]phenyl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
3-[methyl(3-phenylpropyl)amino]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
4-(1-benzoylpiperidin-4-yl)-1-(1,3-oxazol-2-yl)butan-1-one
-
-
4-(1-benzylpiperidin-4-yl)-1-(1,3-oxazol-2-yl)butan-1-one
-
-
4-(2-phenylethoxy)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)butan-1-one
-
-
4-(benzyloxy)phenyl butylcarbamate
O00519, Q6GMR7
;
4-chloromercuribenzoic acid
-
as -SH-blocking reagent, substrate 4-nitroacetanilide, incubation 10 min, 10 micromol 4-chloromercuribenzoic acid, residual activity 4.8%, activity restored by addition of 1 mM cysteine, 1 h incubation 67% restored
4-hydroxy-7-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
4-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)butan-1-one
-
-
4-[1-(2-bromobenzyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
4-[1-(2-chlorobenzyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
4-[1-(2-methoxybenzyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
4-[1-(2-methylbenzyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
4-[1-(3,3-dimethylbutanoyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
4-[1-(3-bromobenzyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
4-[1-(3-chlorobenzyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
4-[1-(3-cyclohexylpropanoyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
4-[1-(3-methoxybenzyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
4-[1-(3-methylbenzyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
4-[1-(4-bromobenzyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
4-[1-(4-chlorobenzyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
4-[1-(4-methoxybenzyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
4-[1-(4-methylbenzyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
4-[1-(benzylsulfonyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
4-[1-(cyclohexylacetyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
4-[1-(cyclohexylcarbonyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
4-[1-(cyclopropylcarbonyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
4-[1-(isopropylsulfonyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
4-[1-[3-(1-methylethoxy)benzyl]piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
4-[1-[3-(1-methylethyl)benzyl]piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
4-[1-[4-(1-methylethoxy)benzyl]piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
4-[1-[4-(1-methylethyl)benzyl]piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
4-[2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazol-5-yl]pyridine-2-carboxylic acid
-
-
4-[methyl(2-phenylethyl)amino]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)butan-1-one
-
-
5-(benzyloxy)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)pentan-1-one
-
-
5-(benzylsulfinyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)pentan-1-one
-
-
5-(benzylthio)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)pentan-1-one
-
-
5-hydroxy-7-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
5-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)pentan-1-one
-
-
5-[2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazol-5-yl]pyridine-2-carboxylic acid
-
-
5-[benzyl(methyl)amino]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)pentan-1-one
-
-
6-(2-[3-[4'-(morpholin-4-ylmethyl)biphenyl-4-yl]propyl]-1,3-oxazol-5-yl)pyridine-2-carboxylic acid
-
-
6-(2-[3-[4'-(piperidin-1-ylmethyl)biphenyl-4-yl]propyl]-1,3-oxazol-5-yl)pyridine-2-carboxylic acid
-
-
6-(2-[3-[4'-(pyrrolidin-1-ylmethyl)biphenyl-4-yl]propyl]-1,3-oxazol-5-yl)pyridine-2-carboxylic acid
-
-
6-(2-[3-[4'-(thiomorpholin-4-ylmethyl)biphenyl-4-yl]propyl]-1,3-oxazol-5-yl)pyridine-2-carboxylic acid
-
-
6-(2-[3-[4-(phenoxymethyl)phenyl]propanoyl]-1,3-oxazol-5-yl)pyridine-2-carbonitrile
-
-
6-(2-[3-[4-(phenoxymethyl)phenyl]propanoyl]-1,3-oxazol-5-yl)pyridine-2-carboxylic acid
-
-
6-(2-[3-[4-(phenoxymethyl)phenyl]propanoyl]-1,3-oxazol-5-yl)pyridine-3-carboxylic acid
-
-
6-(2-[3-[4-(piperidin-4-yloxy)phenyl]propyl]-1,3-oxazol-5-yl)pyridine-2-carboxylic acid
-
-
6-(benzyloxy)-N-[(3S)-2-oxooxetan-3-yl]naphthalene-2-carboxamide
-
-
6-(benzyloxy)-N-[(3S)-2-oxooxetan-3-yl]naphthalene-2-carboxamide
-
-
6-(phenylsulfinyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hexan-1-one
-
-
6-(phenylsulfonyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hexan-1-one
-
-
6-(phenylthio)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hexan-1-one
-
-
6-hydroxy-7-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
6-methoxy-N-[(3S)-2-oxooxetan-3-yl]naphthalene-2-carboxamide
-
-
6-methoxy-N-[(3S)-2-oxooxetan-3-yl]naphthalene-2-carboxamide
-
-
6-oxo-N-phenyl-6-(5-pyridin-2-yl-1,3-oxazol-2-yl)hexanamide
-
-
6-phenoxy-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hexan-1-one
-
-
6-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hexan-1-one
-
-
6-[2-(3-biphenyl-4-yl-1-hydroxypropyl)-1,3-oxazol-5-yl]nicotinic acid
-
-
6-[2-(3-biphenyl-4-yl-1-hydroxypropyl)-1,3-oxazol-5-yl]pyridine-2-carboxamide
-
-
6-[2-(3-biphenyl-4-yl-1-hydroxypropyl)-1,3-oxazol-5-yl]pyridine-2-carboxylic acid
-
-
6-[2-(3-biphenyl-4-yl-1-hydroxypropyl)-1,3-oxazol-5-yl]pyridine-3-carboxamide
-
-
6-[2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazol-5-yl]nicotinamide
-
-
6-[2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazol-5-yl]nicotinic acid
-
-
6-[2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazol-5-yl]pyridine-2-carbonitrile
-
-
6-[2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazol-5-yl]pyridine-2-carboxamide
-
-
6-[2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazol-5-yl]pyridine-2-carboxylic acid
-
-
6-[2-(3-biphenyl-4-ylpropyl)-1,3-oxazol-5-yl]nicotinamide
-
-
6-[2-(3-biphenyl-4-ylpropyl)-1,3-oxazol-5-yl]nicotinic acid
-
-
6-[2-(3-biphenyl-4-ylpropyl)-1,3-oxazol-5-yl]pyridine-2-carboxamide
-
-
6-[2-(3-biphenyl-4-ylpropyl)-1,3-oxazol-5-yl]pyridine-2-carboxylic acid
-
-
6-[2-(3-[4'-[(4-aminopiperazin-1-yl)methyl]biphenyl-4-yl]propyl)-1,3-oxazol-5-yl]pyridine-2-carboxylic acid
-
-
6-[2-(7-phenylheptanoyl)-1,3-oxazol-5-yl]nicotinic acid
-
-
6-[2-(7-phenylheptanoyl)-1,3-oxazol-5-yl]pyridine-2-carbonitrile
-
-
6-[2-(7-phenylheptanoyl)-1,3-oxazol-5-yl]pyridine-2-carboxylic acid
-
-
6-[2-[3-(4-phenoxyphenyl)propanoyl]-1,3-oxazol-5-yl]nicotinic acid
-
-
6-[2-[3-(4-phenoxyphenyl)propanoyl]-1,3-oxazol-5-yl]pyridine-2-carboxylic acid
-
-
6-[2-[3-(4-[[1-(tert-butoxycarbonyl)piperidin-4-yl]oxy]phenyl)propyl]-1,3-oxazol-5-yl]pyridine-2-carboxylic acid
-
-
6-[methyl(phenyl)amino]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hexan-1-one
-
-
7-(2,3-dichlorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-6-yn-1-one
-
-
7-(2,3-dichlorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
7-(2-chlorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-6-yn-1-one
-
-
7-(2-chlorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
7-(2-fluorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-6-yn-1-one
-
-
7-(2-fluorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
7-(2-methoxyphenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
7-(2-methylphenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
7-(2-nitrophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-6-yn-1-one
-
-
7-(3-aminophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
7-(3-chlorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-6-yn-1-one
-
-
7-(3-chlorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
7-(3-fluorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-6-yn-1-one
-
-
7-(3-fluorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
7-(3-methoxyphenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
7-(3-methylphenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
7-(3-nitrophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-6-yn-1-one
-
-
7-(4-aminophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
7-(4-chlorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-6-yn-1-one
-
-
7-(4-chlorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
7-(4-fluorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-6-yn-1-one
-
-
7-(4-fluorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
7-(4-methoxyphenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
7-(4-methylphenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
7-(4-nitrophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-6-yn-1-one
-
-
7-(4-nitrophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
7-hydroxy-7-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
7-naphthalen-1-yl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
7-naphthalen-2-yl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
7-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-6-yn-1-one
-
-
7-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
O00519, Q6GMR7
;
7-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
7-phenyl-1-[5-(trifluoromethyl)-1,3-oxazol-2-yl]heptan-1-one
-
-
7-phenyl-1-[5-[6-(1H-tetrazol-5-yl)pyridin-2-yl]-1,3-oxazol-2-yl]heptan-1-one
-
-
7-pyridin-2-yl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-6-yn-1-one
-
-
7-pyridin-2-yl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
7-pyridin-3-yl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-6-yn-1-one
-
-
7-pyridin-3-yl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
7-pyridin-4-yl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-6-yn-1-one
-
-
7-[2-(methylsulfanyl)phenyl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
7-[3-(methylsulfanyl)phenyl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
7-[3-(tert-butylamino)phenyl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
7-[4-(methylsulfanyl)phenyl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
7-[4-(tert-butylamino)phenyl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
8-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)octan-1-one
-
-
9-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)nonan-1-one
-
-
acetaldehyde
-
competitive inhibitor, not time dependent; competitve inhibitor, Ki: 7.0 micromol
acetaldehyde ammonia
-
-
acetamide
-
non-competitive inhibitor
acetamide
-
no inhibition up to 1.0 M concentration in presence of 0.56 mg protein
acetamide
-
; substrate inhibition
acetanilide
-
substrate 4-nitroacetanilide, Ki: 14 micromol, competitive inhibition
acetic acid
-
competitive product inhibitor at pH 5.7
acetic acid
-
Ki: 240 mM
acetone
-
5% v/v, 86% inhibition; 86% decreased activity at 5% (v/v)
acetone
F8AEP0
25% (v/v), 69% inhibition
acetonitrile
-
5% v/v, 93% inhibition; 93% decreased activity at 5% (v/v)
acetonnitrile
-
Ki: 120 mM
Acrylamide
-
; substrate inhibition
acrylonitrile
-
Ki: 34 mM
Ag+
-
preincubation for 30 min, remaining activity in presence of 1 mM DTT
Ag+
Blastobacter sp.
-
inactivates enzyme activity by 76%
Ag+
-
22% of origin activity
Ag+
-
0% of origin activity
Al3+
-
2-linear mixed-type inhibition of the amidase activity
alpha-methyl-1-arachidonoylglycerol
-
IC50: 0.033 mM, complete inhibition at 0.1 mM
ammonia
-
non-competitive product inhibitor at pH 9.0
arachidonic acid
-
IC50: 0.058 mM, complete inhibition at 0.1 mM
arachidonoyl glycine
-
IC50: 0.0049 mM, complete inhibition at 0.1 mM
arachidonoyl serinol
-
IC50: 0.078 mM, complete inhibition at 0.1 mM
arachidonoyl serotonin
-
more potent inhibitor at pH 8.4 than at pH 5.3
-
aromatic amide
-
-
Ba2+
-
13% inhibition at 1 mM
benzyl 4-[4-(1,3-oxazol-2-yl)-4-oxobutyl]piperidine-1-carboxylate
-
-
Ca2+
-
15% inhibition at 1 mM
Ca2+
-
1-linear competitive inhibition of the amidase activity
Ca2+
F8AEP0
10 mM, 12% inhibition
CaCl2
-
relative activity 91.7%, incubated for 10 min
Cd2+
-
preincubated for 30 min
Cd2+
Blastobacter sp.
-
inactivates enzyme activity by 79%
Chloroacetone
-
pH 8.66, 25C, irreversible strong inhibition at concentrations of 0.1 mM and above, acetamide protects enzyme from inhibition and slowly recovers activity of inactivated enzyme
Co2+
-
complete inhibition at 1 mM
Co2+
-
; 89-48% residual activity at 1 mM
Co2+
F8AEP0
10 mM, 41% inhibition
Cu2+
-
preincubated for 30 min, remaining activity in presence of 1 mM DTT
Cu2+
Blastobacter sp.
-
inactivates enzyme activity by 90%
Cu2+
-
58% of origin activity
Cu2+
-
0% of origin activity
Cu2+
-
relative activity 0%, incubated for 10 min
Cu2+
-
74% inhibition at 1 mM
Cu2+
-
; 89-48% residual activity at 1 mM
Cu2+
F8AEP0
10 mM, 69% inhibition
Cyanate
-
Ki of strain AI3 is 1.1 mM, Ki of strain AIU1N is 1.8 mM and Ki of strain OUCH4 is 50 mM; reversible inhibition, pH 7.2 and 37C
cyclobutylcarbamic acid benzyl ester
-
weak inhibition
cyclobutylcarbamic acid benzyl ester
-
weak inhibition
cyclopentyl(5-pyridin-2-yl-1,3-oxazol-2-yl)methanone
-
-
cyclopropyl(5-pyridin-2-yl-1,3-oxazol-2-yl)methanone
-
-
diethyl dicarbonate
-
-
Diethyl-4-nitrophenyl phosphate
-
-
diisopropylphosphorofluoridate
-
-
Dimethyl sulfide
-
5% v/v, complete inhibition; complete inhibition at 5% (v/v)
dimethyl sulfoxide
-
5% v/v, complete inhibition; complete inhibition at 5% (v/v)
dithiothreitol
-
97% of origin activity
dithiothreitol
-
80% residual activity at 1 mM
DNA containing thymine glycol
O00519, Q02083
-
-
DNA containing thymine glycol
-, O08914, Q9D7V9
-
-
DNA containing thymine glycol
P97612, Q5KTC7
-
-
DNA containing thymine glycol
Q9TUI8
-
-
DTNB
-
preincubated for 30 min, remaining activity in presence of 1 mM DTT
DTNB
-
15-18% inhibition of wild-type, MM6 and MM8, 35% inhibition of the MM15 activity
EDTA
-
10 mM, loss of amidase activity of less than 5%
ethanol
-
58% decreased activity at 5% (v/v); 5% v/v, 58% inhibition
ethanol
F8AEP0
25% (v/v), 45% inhibition
ethyl 4-[3-(4,5-diphenyl-1H-imidazol-1-yl)phenoxy]butanoate
-
-
ethyl 4-[4-(4,5-diphenyl-1H-imidazol-1-yl)phenoxy]butanoate
-
-
ethyl 6-(2-ethyl-4,5-diphenyl-1H-imidazol-1-yl)hexanoate
-
-
ethyl 6-[2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazol-5-yl]pyridine-2-carboxylate
-
-
ethyl 6-[[(3S)-2-oxooxetan-3-yl]carbamoyl]naphthalene-2-carboxylate
-
-
ethyl 6-[[(3S)-2-oxooxetan-3-yl]carbamoyl]naphthalene-2-carboxylate
-
-
ethyl 7-(2-ethyl-4,5-diphenyl-1H-imidazol-1-yl)heptanoate
-
-
ethyl 7-[4,5-bis(4-fluorophenyl)-2-methyl-1H-imidazol-1-yl]heptanoate
-
-
ethyl 8-(2-ethyl-4,5-diphenyl-1H-imidazol-1-yl)octanoate
-
-
Fe2+
-
preincubated for 30 min
Fe2+
-
activity enhanced
Fe2+
-
58% of origin activity
Fe2+
-
20% inhibition at 1 mM
Fe3+
-
; 89-48% residual activity at 1 mM
Fe3+
F8AEP0
10 mM, 68% inhibition
H2O2
-
5% v/v, complete inhibition; complete inhibition at 5% (v/v) H2O2
heptadecanoic acid
-
-
Hg2+
Blastobacter sp.
-
inactivates enzyme activity by 99%
Hg2+
-
7% of the original activity
Hg2+
-
relative activity 91.7%, incubated for 10 min
Hg2+
-
HgCl2 as -SH-blocking reagent, incubation 10 min, 10 micromol HgCl2, 81% of activity restored by addition of 1 mM cysteine
Hg2+
-
complete inhibition at 1 mM
hydroxylamine
-
competitive inhibitor at pH 7.2
Hydroxyurea
-
Ki of strain AI3 is 1.4 mM, Ki of strain AIU1N is 10.8 mM and Ki of strain OUCH4 is 337 mM, regaines full activity within 24 h at 37C in 0.05 M Tris buffer at pH 7.2; reversible inhibition, pH 7.2 and 37C
Ibuprofen
-
both enantiomers, more potent inhibitors at pH 5.3 than at pH 8.4
iodoacetamide
-
hydrolase activity kcat: 0.033 per min with 10 mM at pH 7.0 and 25C, transferase activity 75%, remaining activity less than 2%
iodoacetamide
-
concentration 0.2 M at pH 8.0
iodoacetamide
-
no effect of 2-chloropropionamide
iodoacetamide
-
10% inhibition at 1 mM
iodoacetamide
B4XEY3
-
iodoacetate
-
81% of origin activity
iodoacetate
-
no inhibition, incubated for 10 min
iodoacetate
-
kcat: 0.00325 per min with 250 mM at pH 7.0 and 25C, remaining activity less than 2%
iodoacetate
-
78% inhibition at 1 mM
Isopropanol
-
5% v/v, 60% inhibition; 60% decreased activity at 5% (v/v)
Isopropanol
F8AEP0
25% (v/v), 54% inhibition
isopropyl 4-[4-(1,3-oxazol-2-yl)-4-oxobutyl]piperidine-1-carboxylate
-
-
K+
-
1-linear competitive inhibition of the amidase activity
mercaptoethanol
-
5% v/v, complete inhibition
Methacrylonitrile
-
Ki: 90 mM
methanol
-
50% decreased activity at 5% (v/v); 5% v/v, 50% inhibition
methanol
F8AEP0
25% (v/v), 33% inhibition
methyl 2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazole-5-carboxylate
-
-
methyl 2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazole-5-sulfinate
-
-
methyl 2-(7-phenylheptanoyl)-1,3-oxazole-5-carboxylate
-
-
methyl 2-[3-(4-phenoxyphenyl)propanoyl]-1,3-oxazole-5-carboxylate
-
-
methyl 2-[3-[4-(phenoxymethyl)phenyl]propanoyl]-1,3-oxazole-5-carboxylate
-
-
methyl 2-[7-oxo-7-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-1-yn-1-yl]benzoate
-
-
methyl 2-[7-oxo-7-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptyl]benzenesulfinate
-
-
methyl 2-[7-oxo-7-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptyl]benzoate
-
-
methyl 3-[7-oxo-7-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-1-yn-1-yl]benzoate
-
-
methyl 3-[7-oxo-7-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptyl]benzenesulfinate
-
-
methyl 3-[7-oxo-7-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptyl]benzoate
-
-
methyl 4-[2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazol-5-yl]pyridine-2-carboxylate
-
-
methyl 4-[7-oxo-7-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-1-yn-1-yl]benzoate
-
-
methyl 4-[7-oxo-7-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptyl]benzenesulfinate
-
-
methyl 4-[7-oxo-7-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptyl]benzoate
-
-
methyl 5-oxo-5-(5-pyridin-2-yl-1,3-oxazol-2-yl)pentanoate
-
-
methyl 5-[2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazol-5-yl]pyridine-2-carboxylate
-
-
methyl 6-(2-[3-[4'-(morpholin-4-ylmethyl)biphenyl-4-yl]propyl]-1,3-oxazol-5-yl)pyridine-2-carboxylate
-
-
methyl 6-(2-[3-[4'-(piperidin-1-ylmethyl)biphenyl-4-yl]propyl]-1,3-oxazol-5-yl)pyridine-2-carboxylate
-
-
methyl 6-(2-[3-[4'-(pyrrolidin-1-ylmethyl)biphenyl-4-yl]propyl]-1,3-oxazol-5-yl)pyridine-2-carboxylate
-
-
methyl 6-(2-[3-[4'-(thiomorpholin-4-ylmethyl)biphenyl-4-yl]propyl]-1,3-oxazol-5-yl)pyridine-2-carboxylate
-
-
methyl 6-(2-[3-[4-(phenoxymethyl)phenyl]propanoyl]-1,3-oxazol-5-yl)pyridine-2-carboxylate
-
-
methyl 6-(2-[3-[4-(phenoxymethyl)phenyl]propanoyl]-1,3-oxazol-5-yl)pyridine-3-carboxylate
-
-
methyl 6-(2-[3-[4-(piperidin-4-yloxy)phenyl]propyl]-1,3-oxazol-5-yl)pyridine-2-carboxylate
-
-
methyl 6-oxo-6-(5-pyridin-2-yl-1,3-oxazol-2-yl)hexanoate
-
-
methyl 6-[2-(3-biphenyl-4-yl-1-hydroxypropyl)-1,3-oxazol-5-yl]pyridine-2-carboxylate
-
-
methyl 6-[2-(3-biphenyl-4-yl-1-hydroxypropyl)-1,3-oxazol-5-yl]pyridine-3-carboxylate
-
-
methyl 6-[2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazol-5-yl]pyridine-2-carboxylate
-
-
methyl 6-[2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazol-5-yl]pyridine-3-carboxylate
-
-
methyl 6-[2-(3-biphenyl-4-ylpropyl)-1,3-oxazol-5-yl]pyridine-2-carboxylate
-
-
methyl 6-[2-(3-biphenyl-4-ylpropyl)-1,3-oxazol-5-yl]pyridine-3-carboxylate
-
-
methyl 6-[2-(3-[4'-[(dimethylamino)methyl]biphenyl-4-yl]propyl)-1,3-oxazol-5-yl]pyridine-2-carboxylate
-
-
methyl 6-[2-(7-phenylheptanoyl)-1,3-oxazol-5-yl]pyridine-2-carboxylate
-
-
methyl 6-[2-(7-phenylheptanoyl)-1,3-oxazol-5-yl]pyridine-3-carboxylate
-
-
methyl 6-[2-[3-(4-phenoxyphenyl)propanoyl]-1,3-oxazol-5-yl]pyridine-2-carboxylate
-
-
methyl 6-[2-[3-(4-phenoxyphenyl)propanoyl]-1,3-oxazol-5-yl]pyridine-3-carboxylate
-
-
methyl 6-[2-[3-(4-[[1-(tert-butoxycarbonyl)piperidin-4-yl]oxy]phenyl)propyl]-1,3-oxazol-5-yl]pyridine-2-carboxylate
-
-
methyl [3-(4,5-diphenyl-1H-imidazol-1-yl)phenoxy]acetate
-
-
methyl [4-(4,5-diphenyl-1H-imidazol-1-yl)phenoxy]acetate
-
-
Mg2+
-
partially inhibited
Mg2+
-
relative activity 91.7%, incubated for 10 min
Mg2+
-
10% inhibition at 1 mM
Mg2+
-
1-linear competitive inhibition of the amidase activity
N,N-dimethyl-2-(7-phenylheptanoyl)-1,3-oxazole-5-carboxamide
-
-
N,N-dimethyl-2-[3-(4-phenoxyphenyl)propanoyl]-1,3-oxazole-5-carboxamide
-
-
N,N-dimethyl-2-[3-[4-(phenoxymethyl)phenyl]propanoyl]-1,3-oxazole-5-carboxamide
-
-
N,N-dimethyl-7-(2-methyl-4,5-diphenyl-1H-imidazol-1-yl)heptanamide
-
-
N-(4-hydroxy-2-methylphenyl)arachidonylamide
-
i.e. VDM11, an anandamide uptake inhibitor, IC50 is 0.0026 mM, inhibitory potency depends upon the concentration of bovine serum albumin
N-(4-hydroxyphenyl)arachidonylamide
-
i.e. AM404, an anandamide uptake inhibitor, IC50 is 0.0021 mM
N-(cyclohexylcarbonyl)pentadecylamine
O00519, Q02083
-
N-(cyclohexylcarbonyl)pentadecylamine
-, O08914, Q9D7V9
-
N-(cyclohexylcarbonyl)pentadecylamine
P97612, Q5KTC7
-
N-benzyl-5-oxo-5-(5-pyridin-2-yl-1,3-oxazol-2-yl)pentanamide
-
-
n-butanol
-
48% decreased activity at 5% (v/v); 5% v/v, 48% inhibition
N-ethylmaleimide
-
inhibition at 10 mM, C-amidase
N-methyl-2-(7-phenylheptanoyl)-1,3-oxazole-5-carboxamide
-
-
N-methyl-2-[3-(4-phenoxyphenyl)propanoyl]-1,3-oxazole-5-carboxamide
-
-
N-methyl-2-[3-[4-(phenoxymethyl)phenyl]propanoyl]-1,3-oxazole-5-carboxamide
-
-
N-methyl-7-(2-methyl-4,5-diphenyl-1H-imidazol-1-yl)heptanamide
-
-
N-methylpropionamide
-
amide analogue, inhibits the biosynthesis of acetamidase, strain R312
N-[(3S)-2-oxooxetan-3-yl]biphenyl-4-carboxamide
-
-
N-[(3S)-2-oxooxetan-3-yl]biphenyl-4-carboxamide
-
-
N-[(3S)-2-oxooxetan-3-yl]naphthalene-2-carboxamide
-
-
N-[(3S)-2-oxooxetan-3-yl]naphthalene-2-carboxamide
-
-
N-[(benzyloxy)carbonyl]-D-serine
-
weak inhibition
N-[(benzyloxy)carbonyl]-D-serine
-
weak inhibition
N-[(benzyloxy)carbonyl]-L-serine
-
weak inhibition
N-[(benzyloxy)carbonyl]-L-serine
-
weak inhibition
Na+
-
1-linear competitive inhibition of the amidase activity
NH3
-
selective product inhibition
Ni2+
-
56% inhibition at 1 mM
Ni2+
F8AEP0
10 mM, 31% inhibition
noladin ether
-
IC50: 0.003 mM, complete inhibition at 0.1 mM
o-Nitrophenol
-
competitive inhibition
oleoyltrifluoromethylketone
-
more potent inhibitor at pH 5.3 than at pH 8.4
OMDM2
-
0.01 mM, 16% inhibition
p-chloromercuribenzoate
-
relative activity 36.1%, incubated for 10 min
p-hydroxymercuribenzoate
-
53% of origin activity
p-hydroxymercuribenzoate
-
-
p-hydroxymercuribenzoate
-
inhibition at 10 mM, inhibits the C-amidase
PCMB
O00519, Q02083
-
PCMB
-, O08914, Q9D7V9
-
PCMB
P97612, Q5KTC7
-
Phenacetin
-
substrate 4-nitroacetanilide, Ki: 8.9 nM, competitive inhibition, induction of activity for 2-nitrophenylacetate and methylbutyrate
Phenetidine
-
uncompetitive inhibition
phenyl 6-[[(3S)-2-oxooxetan-3-yl]carbamoyl]naphthalene-2-carboxylate
-
-
phenyl 6-[[(3S)-2-oxooxetan-3-yl]carbamoyl]naphthalene-2-carboxylate
-
-
phenyl [6-(2-methyl-4,5-diphenyl-1H-imidazol-1-yl)hexyl]carbamate
-
-
phenylmethanesulfonyl fluoride
-
-
phenylmethanesulfonyl fluoride
-
inhibits amide conversion more than 94% and completely suppresses nitrile hydrolysis
phenylmethyl sulfonyl fluoride
-
more potent inhibitor at pH 8.4 than at pH 5.3
phenylphosphorodiamidate
-
competitive inhibitor, the inhibitory effect is fully reversed by SSAM substrate benzamide, demonstrating the hydrophobicity of the active site
physostigmine sulfate
-
-
PMSF
O00519, Q02083
-
PMSF
-, O08914, Q9D7V9
-
PMSF
P97612, Q5KTC7
-
PMSF
Q9TUI8
-
Propionic acid
-
-
Propionitrile
-
Ki: 280 mM
specific iron chelator
-
-
-
suberoylanilide hydroxamic acid
Q70I53
strong inhibition
succinate
Blastobacter sp.
-
-
sulfhydyl reagents
Blastobacter sp.
-
-
-
tert-butyl 4-[4-(1,3-oxazol-2-yl)-4-oxobutyl]piperidine-1-carboxylate
-
-
tert-butyl [3-[7-oxo-7-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-1-yn-1-yl]phenyl]carbamate
-
-
tert-butyl [4-[7-oxo-7-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-1-yn-1-yl]phenyl]carbamate
-
-
tetrahydro-2H-pyran-4-yl (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate
-
-
Thioacetamide
-
amide analogues, inhibits the biosynthesis of acetamidase, strain R312
thiol blocking reagent
-
-
-
tricostatin A
Q70I53
strong inhibition
URB597
O00519, Q02083
-
URB597
-, O08914, Q9D7V9
-
URB597
Q9TUI8
-
Urea
-
competitive inhibitor at pH 7.2, 90 mM acetamide protectes AI3 amidase against 2.9 mM urea over 2 h, Ki of strain AI3 is 6.6 mM, Ki of strain AIU1N is 41.3 mM and Ki of strain OUCH4 is not known, regaines full activity within 24 h at 37C in 0.05 M Tris buffer at pH 7.2
Urea
-
competitive inhibitor at pH 7.2, 90 mM acetamide protectes AI3 amidase against 2.9 mM urea over 2 h, Ki of strain AI3 is 6.6 mM, Ki of strain AIU1N is 41.3 mM and Ki of strain OUCH4 is not known, regaines full activity within 24 h at 37C in 0.05 M Tris buffer at pH 7.2; reversible inhibition, pH 7.2 and 37C
Zn2+
Blastobacter sp.
-
inactivates enzyme activity by 88%
Zn2+
-
relative activity 94.4%, incubated for 10 min
Zn2+
-
7% inhibition at 1 mM
Zn2+
-
; 89-48% residual activity at 1 mM
Zn2+
F8AEP0
10 mM, 62% inhibition
MnCl2
-
relative activity 94.4%, incubated for 10 min
additional information
-
no inhibition with 10 mM guanidinium carbonate, 100 mM thiourea, ammonium carbamate at strain AI 3
-
additional information
-
no inhibition by Triton X-100, SDS, EDTA, Mn2+, and Fe3+ at 1 mM
-
additional information
-
cations interact with the active site via Asp189 and the S1 pocket via residue 29, no inhibition by soybean trypsin inhibitor
-
additional information
-
effects of specific monoclonal antibodies on wild-type and mutant enzyme activity
-
additional information
-
inactivation of FAAH might affect the levels of 2-arachidonoylglycerol and, hence, the activity of CB1 receptors
-
additional information
-
diethylphosphoroamidate shows no inhibitory activity
-
additional information
-
the metal ions tested show between 30% and 40% inhibition at 1 mM
-
additional information
-
N-(2-oxo-3-oxetanyl)carbamic acid esters as N-acylethanolamine acid amidase inhibitors, synthesis and structure-activity and structure-property relationships, overview. The beta-lactone ring is responsible for inhibition and and low compound stability. The compounds inhibit NAAA with a rapid, noncompetitive, and reversible mechanism, consistent with the acylation of the catalytic Cys131
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
acetamide
-
induction of acetamidase
dithiothreitol
-
activation to 150% original activity results from addition of dithiothreitol at millimolar concentration
dithiothreitol
O00519, Q02083
activates
dithiothreitol
-, O08914, Q9D7V9
activates
dithiothreitol
P97612, Q5KTC7
activates
DTT
-
20% activation at 1 mM
N-Methylacetamide
-
induction of acetamidase biosynthesis begins at the 3 mM concentration. The induction reaches a maximum plateau between 10 mM and 50 mM
glycine-containing compounds
-
-
-
additional information
-
amide hydrolyzing antibody 312D6 induces catalysis by transition-state mimicry and torsional activation, and accelerates the hydrolysis of amides by a factor of 1000, overview
-
additional information
O00519, Q02083
no activation by dithiothreitol
-
additional information
-, O08914, Q9D7V9
no activation by dithiothreitol
-
additional information
P97612, Q5KTC7
no activation by dithiothreitol
-
additional information
Q9TUI8
no activation by dithiothreitol
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.3
-
2-Aminobenzamide
-
pH 7.5, 70C
1.21
-
2-chloroacetamide
-
pH 7.5, 30C
80
-
2-Chloropropionamide
-
A-amidase
206
-
2-Chloropropionamide
-
strain PPW3, C-amidase
0.4
-
2-hydroxybenzamide
-
pH 7.5, 70C
0.042
-
2-nitroacetanilide
-
-
0.9
-
2-phenylpropionamide
-
pH 7.2, 70C
0.1
-
2-toluamide
-
pH 5.0, 70C, wild-type enzyme
0.125
-
2-toluamide
-
pH 5.0, 70C, wild-type enzyme
0.2
-
2-toluamide
-
pH 5.0, 70C, mutant enzyme Y41C
0.3
-
2-toluamide
-
pH 7.5, 70C
0.9
-
3-indolacetamide
-
pH 7.5, 70C
-
2.325
-
3-phenyl-propionamide
-
pH 5.0, 70C, wild-type enzyme
2.5
-
3-phenylpropionamide
-
pH 5.0, 70C, mutant enzyme Y41C
3
-
3-phenylpropionamide
-
pH 5.0, 70C, wild-type enzyme
4
-
3-phenylpropionamide
-
pH 7.5, 70C
0.3
-
4-Aminobenzamide
-
pH 7.5, 70C
0.1
-
4-hydroxybenzamide
-
pH 5.0, 70C, mutant enzyme Y41C; pH 5.0, 70C, wild-type enzyme
1.5
-
4-hydroxybenzamide
-
pH 7.5, 70C
0.2
-
4-nitrobenzamide
-
pH 5.0, 70C, wild-type enzyme
-
0.3
-
4-nitrobenzamide
-
pH 5.0, 70C, mutant enzyme Y41C
-
0.006
-
4-nitrophenylacetate
-
-
0.315
-
5-[(5-carboxypentanoyl)amino]-2-nitrobenzoic acid
-, Q5YFS2
pH 7.5, 37C
0.27
-
acetamide
-
pH 7.5, 30C
0.8
-
acetamide
-
pH 8.66, 25C
1.2
-
acetamide
-
mutant strain MM8
2.5
-
acetamide
-
strain R312
2.7
-
acetamide
-
-
3.6
-
acetamide
-
pH 7.5, 70C
4
-
acetamide
-
no buffer added, 25C
5.57
-
acetamide
B4XEY3
-
6
-
acetamide
-
pH 7.2, 25C
8.33
-
acetamide
F8AEP0
pH 7.6, 60C
18.2
-
acetamide
-
strain R312, transfer reaction
19
-
acetamide
Q7DKE4
pH 5.9, 55C, recombinant wild-type enzyme
22
-
acetamide
-
pH 5.0, 70C, wild-type enzyme
22.7
-
acetamide
-
pH 7.2
26.5
-
acetamide
-
pH 5.0, 70C, wild-type enzyme
31
-
acetamide
-
-
31.2
-
acetamide
-
-
37
-
acetamide
-
pH 5.0, 70C, mutant enzyme Y41C
68.6
-
acetamide
-
strain AI3
70
-
acetamide
-
acyl transfer reaction on hydroxylamine, pH = 7
90.4
-
acetamide
-
strain AIU1N
100
-
acetamide
-
A-amidase
0.014
-
acetanilide
-
-
0.04
-
acetate-prelabeled chicken histones
Q70I53
-
-
39.2
-
acetic acid
-
-
1.2
-
Acrylamide
-
-
1.7
-
Acrylamide
-
-
2
-
Acrylamide
-
mutant strain MM8
2.39
-
Acrylamide
-
pH 7.5, 30C
3
-
Acrylamide
-
acyl transfer reaction on hydroxylamine, pH = 7
3
-
Acrylamide
-
pH 7.5, 70C
4.79
-
Acrylamide
B4XEY3
-
10.86
-
Acrylamide
F8AEP0
pH 7.6, 60C
16
-
Acrylamide
-
mutant strain 19
24.1
-
Acrylamide
Q7DKE4
pH 5.9, 55C, recombinant wild-type enzyme
43.8
-
Acrylamide
-
pH 7.2
93
-
Acrylamide
-
-
0.0033
-
arachidonoyl-ethanolamine
-
pH 7.3, 37C
0.0036
-
arachidonoyl-ethanolamine
-
pH 6.3, 37C
0.0041
-
arachidonoyl-ethanolamine
-
pH 8.2, 37C
0.000674
-
Benzamide
-
85C, pH not specified in the publication, wild-type enzyme
0.000723
-
Benzamide
-
85C, pH not specified in the publication, mutant enzyme K96R
0.04
-
Benzamide
B4XEY3
-
0.1
-
Benzamide
-
acyl transfer reaction on hydroxylamine, pH = 7
0.15
-
Benzamide
-
-
0.15
-
Benzamide
-
purified wild-type
0.16
-
Benzamide
-
purified C203A mutant
0.3
-
Benzamide
-
pH 7.5, 70C
0.6
-
Benzamide
-
pH 5.0, 70C, mutant enzyme Y41C
0.68
-
Benzamide
-
purified D191E mutant
0.9
-
Benzamide
-
pH 5.0, 70C, wild-type enzyme
1.03
-
Benzamide
Q7DKE4
pH 5.9, 55C, recombinant wild-type enzyme
2.7
-
Benzamide
-
strain R312
4.9
-
Benzamide
-
pH 7.2, 70C
6.5
-
Benzamide
Blastobacter sp.
-
-
7.25
-
Benzamide
-
pH 5.0, 70C, wild-type enzyme
0.127
-
Boc-Lys(epsilon-acetyl)-(4-methyl-coumaryl-7-amide)
Q70I53
pH 7.5, 37C
0.1
-
butyramide
-
acyl transfer reaction on hydroxylamine, pH = 7
0.18
-
butyramide
B4XEY3
-
1.2
-
butyramide
-
pH 7.5, 70C
3.2
-
butyramide
-
-
3.2
-
butyramide
-
pH 5.0, 70C, mutant enzyme Y41C
8.55
-
butyramide
-
pH 5.0, 70C, wild-type enzyme
14
-
butyramide
-
pH 5.0, 70C, wild-type enzyme
0.000434
-
cinnamamide
-
85C, pH not specified in the publication, wild-type enzyme
0.000526
-
cinnamamide
-
85C, pH not specified in the publication, mutant enzyme K96R
4
-
cis-(1S,4R)-N-(4-(hydroxymethyl)cyclopent-2-enyl)acetamide
Q70I53
pH 7.5, 37C
5
-
cyclohexanamide
-
pH 7.5, 70C
-
8
-
DL-Lactamide
-
strain R312
18
-
ethyl acetate
-
-
218
-
ethyl prop-2-enoate
-
-
2.8
-
glutaramic acid
Blastobacter sp.
-
-
0.958
-
glutaryl-nitro-5-amino benzoic acid
-, Q5YFS2
pH 7.5, 37C
0.3
-
hexanamide
-
acyl transfer reaction on hydroxylamine, pH = 7
4.5
-
hexanamide
-
pH 5.0, 70C, mutant enzyme Y41C; pH 5.0, 70C, wild-type enzyme
5.8
-
hexanamide
-
pH 7.5, 70C
11.08
-
hexanamide
F8AEP0
pH 7.6, 60C
131
-
hydroxylamine
-
strain R312, transfer reaction
0.8
-
indol-3-acetamide
-
pH 5.0, 70C, mutant enzyme Y41C; pH 5.0, 70C, wild-type enzyme
1.225
-
indol-3-acetamide
-
pH 5.0, 70C, wild-type enzyme
0.08
-
Indole-3-acetamide
-
-
1.2
-
Indole-3-acetamide
-
-
0.000099
-
Isobutyramide
-
85C, pH not specified in the publication, wild-type enzyme
0.0002
-
Isobutyramide
-
85C, pH not specified in the publication, mutant enzyme K96R
0.1
-
Isobutyramide
-
acyl transfer reaction on hydroxylamine, pH = 7
0.18
-
Isobutyramide
B4XEY3
-
0.53
-
Isobutyramide
-
strain R312
1
-
Isobutyramide
-
pH 7.5, 70C
3.5
-
Isobutyramide
-
-
4.975
-
Isobutyramide
-
pH 5.0, 70C, wild-type enzyme
6.5
-
Isobutyramide
-
pH 5.0, 70C, mutant enzyme Y41C
7
-
Isobutyramide
-
pH 5.0, 70C, wild-type enzyme
0.8
-
Isonicotinamide
-
pH 7.5, 70C
0.000096
-
ketoprofen amide
-
-
0.067
-
ketoprofen amide
-
-
12
-
L-alaninamide
-
acyl transfer reaction on hydroxylamine, pH = 7
15.92
-
L-glutamine
F8AEP0
pH 7.6, 60C
1.3
-
L-Leucinamide
-
acyl transfer reaction on hydroxylamine, pH = 7
5.4
-
L-Methioninamide
-
acyl transfer reaction on hydroxylamine, pH = 7
2
-
L-prolinamide
-
acyl transfer reaction on hydroxylamine, pH = 7
6.7
-
L-prolinamide
-
pH 7.5, 70C
16
-
L-threoninamide
-
acyl transfer reaction on hydroxylamine, pH = 7
3
-
lactamide
-
pH 7.5, 70C
-
0.3
-
metacrylamide
-
pH 5.0, 70C, wild-type enzyme
0.5
-
metacrylamide
-
pH 5.0, 70C, mutant enzyme Y41C
0.55
-
metacrylamide
-
pH 5.0, 70C, wild-type enzyme
1.2
-
methacrylamide
-
pH 7.5, 70C
9.2
-
methacrylamide
-
pH 7.2, 70C
0.3
-
Nicotinamide
-
acyl transfer reaction on hydroxylamine, pH = 7
0.3
-
Nicotinamide
-
pH 7.5, 70C
0.4
-
Nicotinamide
-
pH 5.0, 70C, mutant enzyme Y41C
1.5
-
Nicotinamide
-
pH 5.0, 70C, wild-type enzyme
0.5
-
p-nitroacetanilide
P11436
strain AI3
0.63
-
p-nitroacetanilide
P11436
strain 10A
1.17
-
p-nitroacetanilide
P11436
strain 8A
1.98
-
p-nitroacetanilide
P11436
strain 3B
1.3
-
Pentanamide
-
pH 7.5, 70C
1.5
-
Pentanamide
-
pH 5.0, 70C, mutant enzyme Y41C
2.5
-
Pentanamide
-
pH 5.0, 70C, wild-type enzyme
0.069
-
phenylacetamide
-
-
0.029
-
phenylacetate
-
-
2.5
-
picolinamide
-
pH 7.5, 70C
-
1.1
-
pivalamide
-
strain R312
0.000093
-
Propionamide
-
85C, pH not specified in the publication, mutant enzyme K96R
0.000102
-
Propionamide
-
85C, pH not specified in the publication, wild-type enzyme
0.48
-
Propionamide
-
-
1
-
Propionamide
-
acyl transfer reaction on hydroxylamine, pH = 7
1.3
-
Propionamide
B4XEY3
-
1.3
-
Propionamide
-
pH 7.5, 70C
2.6
-
Propionamide
-
-
4.29
-
Propionamide
-
pH 7.5, 30C
7.4
-
Propionamide
-
pH 7.2, 70C
10
-
Propionamide
-
pH 5.0, 70C, mutant enzyme Y41C; pH 5.0, 70C, wild-type enzyme
12.25
-
Propionamide
-
pH 5.0, 70C, wild-type enzyme
34.2
-
Propionamide
Q7DKE4
pH 5.9, 55C, recombinant wild-type enzyme
44
-
Propionamide
-
strain R312
88
-
Propionamide
-
-
628
-
Propionate
-
-
16
-
propionic acid ethyl ester
-
-
6.2
-
succinamic acid
Blastobacter sp.
-
-
1.88
-
Urea
-
pH 7.5, 30C
0.24
-
Valeramide
-
strain R312
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.9
-
2-Aminobenzamide
-
pH 7.5, 70C
1.3
-
2-hydroxybenzamide
-
pH 7.5, 70C
129
-
2-nitroacetanilide
-
-
0.3
-
2-toluamide
-
pH 7.5, 70C
0.4
-
2-toluamide
-
pH 5.0, 70C, wild-type enzyme
0.425
-
2-toluamide
-
pH 5.0, 70C, wild-type enzyme
0.8
-
2-toluamide
-
pH 5.0, 70C, mutant enzyme Y41C
1.8
-
3-indolacetamide
-
pH 7.5, 70C
-
12.82
-
3-phenyl-propionamide
-
pH 5.0, 70C, wild-type enzyme
7.1
-
3-phenylpropionamide
-
pH 7.5, 70C
15.3
-
3-phenylpropionamide
-
pH 5.0, 70C, wild-type enzyme
18
-
3-phenylpropionamide
-
pH 5.0, 70C, mutant enzyme Y41C
0.8
-
4-Aminobenzamide
-
pH 7.5, 70C
1.1
-
4-hydroxybenzamide
-
pH 7.5, 70C
1.9
-
4-hydroxybenzamide
-
pH 5.0, 70C, wild-type enzyme
2.1
-
4-hydroxybenzamide
-
pH 5.0, 70C, mutant enzyme Y41C
0.8
-
4-nitrobenzamide
-
pH 5.0, 70C, wild-type enzyme
-
2.5
-
4-nitrobenzamide
-
pH 5.0, 70C, mutant enzyme Y41C
-
144
-
4-nitrophenylacetate
-
-
0.143
-
acetamide
-
hydrolysis, strain AI3
0.21
-
acetamide
B4XEY3
-
1.51
-
acetamide
-
hydrolysis, strain AIU1N
1.6
-
acetamide
-
pH 7.5, 70C
20.13
-
acetamide
-
pH 5.0, 70C, wild-type enzyme
20.2
-
acetamide
Q7DKE4
pH 5.9, 55C, recombinant wild-type enzyme
28.9
-
acetamide
-
pH 5.0, 70C, wild-type enzyme
30.1
-
acetamide
-
pH 5.0, 70C, mutant enzyme Y41C
162
-
acetamide
-
hydrolysis
305
-
acetamide
-
-
790
-
acetamide
-
transferase reaction
2668
-
acetamide
-
pH 7.2
5051
-
acetamide
F8AEP0
pH 7.6, 60C
134
-
acetanilide
-
-
47.4
-
acetate
-
-
10
-
acetohydrazide
-
hydrolysis
20
-
acetohydrazide
-
transferase reaction
14
-
Acetohydroxamate
-
hydrolysis
0.58
-
Acrylamide
B4XEY3
-
2.1
-
Acrylamide
Q7DKE4
pH 5.9, 55C, recombinant wild-type enzyme
8.3
-
Acrylamide
-
pH 7.5, 70C
38.7
-
Acrylamide
-
pH 7.2
333.3
-
Acrylamide
F8AEP0
pH 7.6, 60C
0.00217
-
Benzamide
Blastobacter sp.
-
-
0.48
-
Benzamide
B4XEY3
-
6.1
-
Benzamide
-
pH 5.0, 70C, wild-type enzyme
6.4
-
Benzamide
-
pH 7.5, 70C
6.7
-
Benzamide
-
pH 5.0, 70C, wild-type enzyme
7.2
-
Benzamide
-
pH 5.0, 70C, mutant enzyme Y41C
157.7
-
Benzamide
Q7DKE4
pH 5.9, 55C, recombinant wild-type enzyme
1192
-
Benzamide
-
85C, pH not specified in the publication, wild-type enzyme
1246
-
Benzamide
-
85C, pH not specified in the publication, mutant enzyme K96R
2.04
-
butyramide
B4XEY3
-
9.9
-
butyramide
-
pH 7.5, 70C
17.5
-
butyramide
-
pH 5.0, 70C, mutant enzyme Y41C
19.98
-
butyramide
-
pH 5.0, 70C, wild-type enzyme
34.3
-
butyramide
-
pH 5.0, 70C, wild-type enzyme
769
-
cinnamamide
-
85C, pH not specified in the publication, wild-type enzyme
906
-
cinnamamide
-
85C, pH not specified in the publication, mutant enzyme K96R
16.3
-
cyclohexanamide
-
pH 7.5, 70C
-
0.0372
-
glutaramic acid
Blastobacter sp.
-
-
5.3
-
hexanamide
-
pH 7.5, 70C
19.5
-
hexanamide
-
pH 5.0, 70C, wild-type enzyme
21.6
-
hexanamide
-
pH 5.0, 70C, mutant enzyme Y41C
721.7
-
hexanamide
F8AEP0
pH 7.6, 60C
1.2
-
indol-3-acetamide
-
pH 5.0, 70C, mutant enzyme Y41C
2.05
-
indol-3-acetamide
-
pH 5.0, 70C, wild-type enzyme
2.2
-
indol-3-acetamide
-
pH 5.0, 70C, wild-type enzyme
5.68
-
Isobutyramide
B4XEY3
-
21.9
-
Isobutyramide
-
pH 7.5, 70C
41.88
-
Isobutyramide
-
pH 5.0, 70C, wild-type enzyme
48.9
-
Isobutyramide
-
pH 5.0, 70C, wild-type enzyme
58.4
-
Isobutyramide
-
pH 5.0, 70C, mutant enzyme Y41C
160
-
Isobutyramide
-
85C, pH not specified in the publication, mutant enzyme K96R
175
-
Isobutyramide
-
85C, pH not specified in the publication, wild-type enzyme
1.6
-
Isonicotinamide
-
pH 7.5, 70C
887
-
ketoprofen amide
-
-
7216
-
L-glutamine
F8AEP0
pH 7.6, 60C
3.1
-
L-prolinamide
-
pH 7.5, 70C
10.7
-
lactamide
-
pH 7.5, 70C
-
3
-
metacrylamide
-
pH 5.0, 70C, mutant enzyme Y41C
4.8
-
metacrylamide
-
pH 5.0, 70C, wild-type enzyme
5.75
-
metacrylamide
-
pH 5.0, 70C, wild-type enzyme
2.9
-
methacrylamide
-
pH 7.5, 70C
1.08
-
N-Methylacetamide
-
hydrolysis
890
-
N-Methylacetamide
-
transferase reaction
0.3
-
N-methylpropionamide
-
hydrolysis
1.6
-
Nicotinamide
-
pH 7.5, 70C
2.2
-
Nicotinamide
-
pH 5.0, 70C, mutant enzyme Y41C
6.4
-
Nicotinamide
-
pH 5.0, 70C, wild-type enzyme
20.5
-
Pentanamide
-
pH 7.5, 70C
26.8
-
Pentanamide
-
pH 5.0, 70C, wild-type enzyme
28.6
-
Pentanamide
-
pH 5.0, 70C, mutant enzyme Y41C
147
-
phenylacetate
-
-
1.8
-
picolinamide
-
pH 7.5, 70C
-
3.2
-
Propionamide
-
pH 7.5, 70C
4.03
-
Propionamide
B4XEY3
-
10.5
-
Propionamide
-
-
82.9
-
Propionamide
-
pH 5.0, 70C, wild-type enzyme
99.4
-
Propionamide
-
pH 5.0, 70C, mutant enzyme Y41C
101.9
-
Propionamide
-
pH 5.0, 70C, wild-type enzyme
155.3
-
Propionamide
Q7DKE4
pH 5.9, 55C, recombinant wild-type enzyme
160
-
Propionamide
-
85C, pH not specified in the publication, mutant enzyme K96R
181
-
Propionamide
-
85C, pH not specified in the publication, wild-type enzyme
374
-
Propionamide
-
hydrolysis
465
-
Propionamide
-
transferase reaction
9.5
-
propionohydrazide
-
transferase reaction
22
-
propionohydroxamate
-
hydrolysis
0.096
-
succinamic acid
Blastobacter sp.
-
-
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.3
-
2-Aminobenzamide
-
pH 7.5, 70C
10475
3.3
-
2-hydroxybenzamide
-
pH 7.5, 70C
16901
0.75
-
2-toluamide
-
pH 7.5, 70C
8914
3.25
-
2-toluamide
-
pH 5.0, 70C, wild-type enzyme
8914
4
-
2-toluamide
-
pH 5.0, 70C, mutant enzyme Y41C; pH 5.0, 70C, wild-type enzyme
8914
2
-
3-indolacetamide
-
pH 7.5, 70C
0
5.44
-
3-phenyl-propionamide
-
pH 5.0, 70C, wild-type enzyme
41356
1.8
-
3-phenylpropionamide
-
pH 7.5, 70C
7590
5.1
-
3-phenylpropionamide
-
pH 5.0, 70C, wild-type enzyme
7590
7.2
-
3-phenylpropionamide
-
pH 5.0, 70C, mutant enzyme Y41C
7590
0.27
-
4-Aminobenzamide
-
pH 7.5, 70C
9048
0.73
-
4-hydroxybenzamide
-
pH 7.5, 70C
6781
19
-
4-hydroxybenzamide
-
pH 5.0, 70C, wild-type enzyme
6781
21
-
4-hydroxybenzamide
-
pH 5.0, 70C, mutant enzyme Y41C
6781
4
-
4-nitrobenzamide
-
pH 5.0, 70C, wild-type enzyme
0
8.33
-
4-nitrobenzamide
-
pH 5.0, 70C, mutant enzyme Y41C
0
0.44
-
acetamide
-
pH 7.5, 70C
1057
0.76
-
acetamide
-
pH 5.0, 70C, wild-type enzyme
1057
0.81
-
acetamide
-
pH 5.0, 70C, mutant enzyme Y41C
1057
1.14
-
acetamide
Q7DKE4
pH 5.9, 55C, recombinant wild-type enzyme
1057
1.31
-
acetamide
-
pH 5.0, 70C, wild-type enzyme
1057
606.3
-
acetamide
F8AEP0
pH 7.6, 60C
1057
0.087
-
Acrylamide
Q7DKE4
pH 5.9, 55C, recombinant wild-type enzyme
1914
2.8
-
Acrylamide
-
pH 7.5, 70C
1914
30.69
-
Acrylamide
F8AEP0
pH 7.6, 60C
1914
7.44
-
Benzamide
-
pH 5.0, 70C, wild-type enzyme
1408
10.4
-
Benzamide
-
pH 5.0, 70C, wild-type enzyme
1408
12
-
Benzamide
-
pH 5.0, 70C, mutant enzyme Y41C
1408
21.3
-
Benzamide
-
pH 7.5, 70C
1408
153.5
-
Benzamide
Q7DKE4
pH 5.9, 55C, recombinant wild-type enzyme
1408
2.45
-
butyramide
-
pH 5.0, 70C, wild-type enzyme
3331
2.8
-
butyramide
-
pH 5.0, 70C, wild-type enzyme
3331
5.47
-
butyramide
-
pH 5.0, 70C, mutant enzyme Y41C
3331
8.25
-
butyramide
-
pH 7.5, 70C
3331
3.3
-
cyclohexanamide
-
pH 7.5, 70C
0
0.91
-
hexanamide
-
pH 7.5, 70C
5775
4.33
-
hexanamide
-
pH 5.0, 70C, wild-type enzyme
5775
4.8
-
hexanamide
-
pH 5.0, 70C, mutant enzyme Y41C
5775
65.14
-
hexanamide
F8AEP0
pH 7.6, 60C
5775
1.5
-
indol-3-acetamide
-
pH 5.0, 70C, mutant enzyme Y41C
12151
2.125
-
indol-3-acetamide
-
pH 5.0, 70C, wild-type enzyme
12151
2.75
-
indol-3-acetamide
-
pH 5.0, 70C, wild-type enzyme
12151
6.99
-
Isobutyramide
-
pH 5.0, 70C, wild-type enzyme
2339
7.5
-
Isobutyramide
-
pH 5.0, 70C, wild-type enzyme
2339
8.98
-
Isobutyramide
-
pH 5.0, 70C, mutant enzyme Y41C
2339
21.9
-
Isobutyramide
-
pH 7.5, 70C
2339
2
-
Isonicotinamide
-
pH 7.5, 70C
12567
453.2
-
L-glutamine
F8AEP0
pH 7.6, 60C
102
0.46
-
L-prolinamide
-
pH 7.5, 70C
13695
3.57
-
lactamide
-
pH 7.5, 70C
0
6
-
metacrylamide
-
pH 5.0, 70C, mutant enzyme Y41C
12150
11.81
-
metacrylamide
-
pH 5.0, 70C, wild-type enzyme
12150
16
-
metacrylamide
-
pH 5.0, 70C, wild-type enzyme
12150
2.4
-
methacrylamide
-
pH 7.5, 70C
9670
4.26
-
Nicotinamide
-
pH 5.0, 70C, wild-type enzyme
267
5.3
-
Nicotinamide
-
pH 7.5, 70C
267
5.5
-
Nicotinamide
-
pH 5.0, 70C, mutant enzyme Y41C
267
10.7
-
Pentanamide
-
pH 5.0, 70C, wild-type enzyme
6010
15.7
-
Pentanamide
-
pH 7.5, 70C
6010
19
-
Pentanamide
-
pH 5.0, 70C, mutant enzyme Y41C
6010
0.72
-
picolinamide
-
pH 7.5, 70C
0
2.46
-
Propionamide
-
pH 7.5, 70C
1799
4.54
-
Propionamide
Q7DKE4
pH 5.9, 55C, recombinant wild-type enzyme
1799
7.41
-
Propionamide
-
pH 5.0, 70C, wild-type enzyme
1799
8.29
-
Propionamide
-
pH 5.0, 70C, wild-type enzyme
1799
9.94
-
Propionamide
-
pH 5.0, 70C, mutant enzyme Y41C
1799
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.000077
-
1-(1,3-oxazol-2-yl)-3-(4-phenoxyphenyl)propan-1-one
-
-
0.000025
-
1-(1,3-oxazol-2-yl)-3-[4-(phenoxymethyl)phenyl]propan-1-one
-
-
0.000048
-
1-(1,3-oxazol-2-yl)-7-phenylheptan-1-one
-
-
0.00001
-
1-(5-iodo-1,3-oxazol-2-yl)-3-(4-phenoxyphenyl)propan-1-one
-
-
0.000004
-
1-(5-iodo-1,3-oxazol-2-yl)-3-[4-(phenoxymethyl)phenyl]propan-1-one
-
-
0.000003
-
1-(5-iodo-1,3-oxazol-2-yl)-7-phenylheptan-1-one
-
-
0.0000043
-
1-(5-pyridin-2-yl-1,3-oxazol-2-yl)-7-(2-thienyl)heptan-1-one
-
-
0.0000051
-
1-(5-pyridin-2-yl-1,3-oxazol-2-yl)-7-(3-thienyl)heptan-1-one
-
-
0.000016
-
1-(5-pyridin-2-yl-1,3-oxazol-2-yl)-7-[2-(trifluoromethyl)phenyl]hept-6-yn-1-one
-
-
0.000004
-
1-(5-pyridin-2-yl-1,3-oxazol-2-yl)-7-[2-(trifluoromethyl)phenyl]heptan-1-one
-
-
0.00001
-
1-(5-pyridin-2-yl-1,3-oxazol-2-yl)-7-[3-(trifluoromethyl)phenyl]hept-6-yn-1-one
-
-
0.000001
-
1-(5-pyridin-2-yl-1,3-oxazol-2-yl)-7-[3-(trifluoromethyl)phenyl]heptan-1-one
-
-
0.000075
-
1-(5-pyridin-2-yl-1,3-oxazol-2-yl)-7-[4-(trifluoromethyl)phenyl]hept-6-yn-1-one
-
-
0.000004
-
1-(5-pyridin-2-yl-1,3-oxazol-2-yl)-7-[4-(trifluoromethyl)phenyl]heptan-1-one
-
-
0.000025
-
1-([4'-[3-(1,3-oxazol-2-yl)propyl]biphenyl-4-yl]methyl)piperidine
-
-
0.01
-
1-[6-(2-methyl-4,5-diphenyl-1H-imidazol-1-yl)hexyl]-3-phenylurea
-
-
0.000022
-
11-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)undecan-1-one
-
-
0.000024
-
2,6-difluorophenyl [6-[4,5-bis(4-fluorophenyl)-2-methyl-1H-imidazol-1-yl]hexyl]carbamate
-
-
0.0000002
-
2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazole-5-carbonitrile
-
-
0.0000035
-
2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazole-5-carboxamide
-
-
0.00001
-
2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazole-5-carboxylic acid
-
-
0.000003
-
2-(3-biphenyl-4-ylpropanoyl)-N,N-dimethyl-1,3-oxazole-5-carboxamide
-
-
0.00006
-
2-(4-phenylbutoxy)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)ethanone
-
-
0.0000004
-
2-(7-phenylheptanoyl)-1,3-oxazole-5-carbonitrile
-
-
0.000005
-
2-(7-phenylheptanoyl)-1,3-oxazole-5-carboxamide
-
-
0.00003
-
2-(7-phenylheptanoyl)-1,3-oxazole-5-carboxylic acid
-
-
0.0008
-
2-chloro-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)ethanone
-
-
0.00003
-
2-fluorophenyl [1-ethyl-6-(2-methyl-4,5-diphenyl-1H-imidazol-1-yl)hexyl]carbamate
-
-
0.0000042
-
2-fluorophenyl [1-methyl-6-(2-methyl-4,5-diphenyl-1H-imidazol-1-yl)hexyl]carbamate
-
-
0.00025
-
2-fluorophenyl [6-(2-methyl-4,5-diphenyl-1H-imidazol-1-yl)-1-(1-methylethyl)hexyl]carbamate
-
-
0.00011
-
2-fluorophenyl [6-(2-methyl-4,5-diphenyl-1H-imidazol-1-yl)-1-phenylhexyl]carbamate
-
-
0.000002
-
2-fluorophenyl [6-(2-methyl-4,5-diphenyl-1H-imidazol-1-yl)hexyl]carbamate
-
-
0.00001
-
2-fluorophenyl [6-[4,5-bis(4-fluorophenyl)-2-methyl-1H-imidazol-1-yl]hexyl]carbamate
-
-
0.000008
-
2-hydroxy-7-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.001
-
2-[(4-phenylbutyl)sulfinyl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)ethanone
-
-
0.02
-
2-[(4-phenylbutyl)sulfonyl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)ethanone
-
-
0.000004
-
2-[(4-phenylbutyl)thio]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)ethanone
-
-
0.0000008
-
2-[3-(4-phenoxyphenyl)propanoyl]-1,3-oxazole-5-carbonitrile
-
-
0.00000085
-
2-[3-(4-phenoxyphenyl)propanoyl]-1,3-oxazole-5-carbonitrile
-
-
0.0000066
-
2-[3-(4-phenoxyphenyl)propanoyl]-1,3-oxazole-5-carboxamide
-
-
0.000041
-
2-[3-(4-phenoxyphenyl)propanoyl]-1,3-oxazole-5-carboxylic acid
-
-
0.0021
-
2-[3-(benzyloxy)phenoxy]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)ethanone
-
-
0.0000005
-
2-[3-[4-(phenoxymethyl)phenyl]propanoyl]-1,3-oxazole-5-carbonitrile
-
-
0.00000051
-
2-[3-[4-(phenoxymethyl)phenyl]propanoyl]-1,3-oxazole-5-carbonitrile
-
-
0.0000045
-
2-[3-[4-(phenoxymethyl)phenyl]propanoyl]-1,3-oxazole-5-carboxamide
-
-
0.000022
-
2-[3-[4-(phenoxymethyl)phenyl]propanoyl]-1,3-oxazole-5-carboxylic acid
-
-
0.0053
-
2-[4-(benzyloxy)phenoxy]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)ethanone
-
-
0.002
-
2-[methyl(4-phenylbutyl)amino]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)ethanol
-
-
0.00000075
-
3-(2-naphthyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
0.00009
-
3-(3-phenylpropoxy)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
0.000002
-
3-(4-anilinophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
0.0000032
-
3-(4-benzylphenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
0.0000034
-
3-(4-phenoxyphenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
0.000001
-
3-(4-phenoxyphenyl)-1-[5-(trifluoromethyl)-1,3-oxazol-2-yl]propan-1-one
-
-
0.000002
-
3-(4-phenoxyphenyl)-1-[5-(trifluoromethyl)-1,3-oxazol-2-yl]propan-1-one
-
-
0.000016
-
3-biphenyl-4-yl-1-(1,3-oxazol-2-yl)propan-1-one
-
-
0.000004
-
3-biphenyl-4-yl-1-(5-iodo-1,3-oxazol-2-yl)propan-1-one
-
-
0.00000075
-
3-biphenyl-4-yl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
0.0000029
-
3-biphenyl-4-yl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
0.0000011
-
3-biphenyl-4-yl-1-(5-pyridin-3-yl-1,3-oxazol-2-yl)propan-1-one
-
-
0.0000023
-
3-biphenyl-4-yl-1-(5-pyridin-4-yl-1,3-oxazol-2-yl)propan-1-one
-
-
0.000003
-
3-biphenyl-4-yl-1-[5-(methylthio)-1,3-oxazol-2-yl]propan-1-one
-
-
0.000008
-
3-biphenyl-4-yl-1-[5-(trifluoroacetyl)-1,3-oxazol-2-yl]propan-1-one
-
-
0.0000007
-
3-biphenyl-4-yl-1-[5-(trifluoromethyl)-1,3-oxazol-2-yl]propan-1-one
-
-
0.000025
-
3-biphenyl-4-yl-1-[5-[6-(1H-tetrazol-1-yl)pyridin-2-yl]-1,3-oxazol-2-yl]propan-1-one
-
-
0.000035
-
3-hydroxy-7-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.000025
-
3-[3-(benzyloxy)phenyl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
0.00000038
-
3-[4-(benzyloxy)cyclohexa-1,5-dien-1-yl]-1-[1,3]oxazolo[4,5-b]pyridin-2-ylpropan-1-one
-
-
0.00000045
-
3-[4-(benzyloxy)phenyl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
0.0000013
-
3-[4-(benzyloxy)phenyl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
0.000001
-
3-[4-(phenoxymethyl)phenyl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
0.000005
-
3-[4-(phenoxymethyl)phenyl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
0.00000067
-
3-[4-(phenoxymethyl)phenyl]-1-[5-(trifluoromethyl)-1,3-oxazol-2-yl]propan-1-one
-
-
0.0000008
-
3-[4-(phenoxymethyl)phenyl]-1-[5-(trifluoromethyl)-1,3-oxazol-2-yl]propan-1-one
-
-
0.000021
-
3-[4-(phenoxymethyl)phenyl]-1-[5-[6-(1H-tetrazol-5-yl)pyridin-2-yl]-1,3-oxazol-2-yl]propan-1-one
-
-
0.0000022
-
3-[4-(phenylthio)phenyl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
0.000001
-
3-[4-[hydroxy(phenyl)methyl]cyclohexa-1,5-dien-1-yl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
0.000005
-
3-[4-[hydroxy(phenyl)methyl]cyclohexa-1,5-dien-1-yl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
0.000001
-
3-[4-[hydroxy(phenyl)methyl]phenyl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
0.0002
-
3-[methyl(3-phenylpropyl)amino]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)propan-1-one
-
-
0.000063
-
4-(2-phenylethoxy)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)butan-1-one
-
-
0.0005
-
4-hydroxy-7-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.00012
-
4-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)butan-1-one
-
-
0.00005
-
4-[2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazol-5-yl]pyridine-2-carboxylic acid
-
-
0.0035
-
4-[methyl(2-phenylethyl)amino]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)butan-1-one
-
-
0.000055
-
5-(benzyloxy)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)pentan-1-one
-
-
0.0025
-
5-(benzylsulfinyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)pentan-1-one
-
-
0.0000006
-
5-(benzylthio)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)pentan-1-one
-
-
0.000025
-
5-(benzylthio)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)pentan-1-one
-
-
0.0015
-
5-hydroxy-7-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.000033
-
5-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)pentan-1-one
-
-
0.000044
-
5-[2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazol-5-yl]pyridine-2-carboxylic acid
-
-
0.0000029
-
5-[benzyl(methyl)amino]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)pentan-1-one
-
-
0.0002
-
5-[benzyl(methyl)amino]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)pentan-1-one
-
-
0.00015
-
6-(2-[3-[4'-(morpholin-4-ylmethyl)biphenyl-4-yl]propyl]-1,3-oxazol-5-yl)pyridine-2-carboxylic acid
-
-
0.000033
-
6-(2-[3-[4'-(piperidin-1-ylmethyl)biphenyl-4-yl]propyl]-1,3-oxazol-5-yl)pyridine-2-carboxylic acid
-
-
0.00013
-
6-(2-[3-[4'-(pyrrolidin-1-ylmethyl)biphenyl-4-yl]propyl]-1,3-oxazol-5-yl)pyridine-2-carboxylic acid
-
-
0.000028
-
6-(2-[3-[4'-(thiomorpholin-4-ylmethyl)biphenyl-4-yl]propyl]-1,3-oxazol-5-yl)pyridine-2-carboxylic acid
-
-
0.0000003
-
6-(2-[3-[4-(phenoxymethyl)phenyl]propanoyl]-1,3-oxazol-5-yl)pyridine-2-carbonitrile
-
-
0.000006
-
6-(2-[3-[4-(phenoxymethyl)phenyl]propanoyl]-1,3-oxazol-5-yl)pyridine-2-carboxylic acid
-
-
0.0000046
-
6-(2-[3-[4-(phenoxymethyl)phenyl]propanoyl]-1,3-oxazol-5-yl)pyridine-3-carboxylic acid
-
-
0.011
-
6-(2-[3-[4-(piperidin-4-yloxy)phenyl]propyl]-1,3-oxazol-5-yl)pyridine-2-carboxylic acid
-
-
0.001
-
6-(phenylsulfinyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hexan-1-one
-
-
0.006
-
6-(phenylsulfonyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hexan-1-one
-
-
0.000003
-
6-(phenylthio)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hexan-1-one
-
-
0.0002
-
6-hydroxy-7-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.0031
-
6-oxo-N-phenyl-6-(5-pyridin-2-yl-1,3-oxazol-2-yl)hexanamide
-
-
0.000014
-
6-phenoxy-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hexan-1-one
-
-
0.000011
-
6-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hexan-1-one
-
-
0.01
-
6-[2-(3-biphenyl-4-yl-1-hydroxypropyl)-1,3-oxazol-5-yl]nicotinic acid
-
-
0.02
-
6-[2-(3-biphenyl-4-yl-1-hydroxypropyl)-1,3-oxazol-5-yl]pyridine-2-carboxamide
-
-
0.0011
-
6-[2-(3-biphenyl-4-yl-1-hydroxypropyl)-1,3-oxazol-5-yl]pyridine-2-carboxylic acid
-
-
0.0000013
-
6-[2-(3-biphenyl-4-yl-1-hydroxypropyl)-1,3-oxazol-5-yl]pyridine-3-carboxamide
-
-
0.0000013
-
6-[2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazol-5-yl]nicotinamide
-
-
0.000014
-
6-[2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazol-5-yl]nicotinic acid
-
-
0.0000029
-
6-[2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazol-5-yl]pyridine-2-carbonitrile
-
-
0.0000012
-
6-[2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazol-5-yl]pyridine-2-carboxamide
-
-
0.000022
-
6-[2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazol-5-yl]pyridine-2-carboxylic acid
-
-
0.03
-
6-[2-(3-biphenyl-4-ylpropyl)-1,3-oxazol-5-yl]nicotinamide
-
-
0.02
-
6-[2-(3-biphenyl-4-ylpropyl)-1,3-oxazol-5-yl]nicotinic acid
-
-
0.004
-
6-[2-(3-biphenyl-4-ylpropyl)-1,3-oxazol-5-yl]pyridine-2-carboxamide
-
-
0.01
-
6-[2-(3-biphenyl-4-ylpropyl)-1,3-oxazol-5-yl]pyridine-2-carboxylic acid
-
-
0.0012
-
6-[2-(3-[4'-[(4-aminopiperazin-1-yl)methyl]biphenyl-4-yl]propyl)-1,3-oxazol-5-yl]pyridine-2-carboxylic acid
-
-
0.000007
-
6-[2-(7-phenylheptanoyl)-1,3-oxazol-5-yl]nicotinic acid
-
-
0.0000029
-
6-[2-(7-phenylheptanoyl)-1,3-oxazol-5-yl]pyridine-2-carbonitrile
-
-
0.00002
-
6-[2-(7-phenylheptanoyl)-1,3-oxazol-5-yl]pyridine-2-carboxylic acid
-
-
0.000033
-
6-[2-[3-(4-phenoxyphenyl)propanoyl]-1,3-oxazol-5-yl]nicotinic acid
-
-
0.000065
-
6-[2-[3-(4-phenoxyphenyl)propanoyl]-1,3-oxazol-5-yl]pyridine-2-carboxylic acid
-
-
0.00012
-
6-[2-[3-(4-[[1-(tert-butoxycarbonyl)piperidin-4-yl]oxy]phenyl)propyl]-1,3-oxazol-5-yl]pyridine-2-carboxylic acid
-
-
0.00004
-
6-[methyl(phenyl)amino]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hexan-1-one
-
-
0.000005
-
7-(2,3-dichlorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-6-yn-1-one
-
-
0.0000009
-
7-(2,3-dichlorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.0000012
-
7-(2,3-dichlorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.000013
-
7-(2-chlorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-6-yn-1-one
-
-
0.0000019
-
7-(2-chlorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.000008
-
7-(2-fluorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-6-yn-1-one
-
-
0.0000017
-
7-(2-fluorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.0000058
-
7-(2-methoxyphenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.000003
-
7-(2-methylphenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.000017
-
7-(2-nitrophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-6-yn-1-one
-
-
0.00003
-
7-(3-aminophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.000007
-
7-(3-chlorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-6-yn-1-one
-
-
0.0000009
-
7-(3-chlorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.0000032
-
7-(3-fluorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-6-yn-1-one
-
-
0.0000022
-
7-(3-fluorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.0000025
-
7-(3-methoxyphenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.0000033
-
7-(3-methylphenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.00000048
-
7-(3-nitrophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-6-yn-1-one
-
-
0.000003
-
7-(4-aminophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.00002
-
7-(4-chlorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-6-yn-1-one
-
-
0.000027
-
7-(4-chlorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.000036
-
7-(4-fluorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-6-yn-1-one
-
-
0.0000032
-
7-(4-fluorophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.0000062
-
7-(4-methoxyphenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.0000028
-
7-(4-methylphenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.000031
-
7-(4-nitrophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-6-yn-1-one
-
-
0.0000065
-
7-(4-nitrophenyl)-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.000035
-
7-hydroxy-7-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.0000026
-
7-naphthalen-1-yl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.000011
-
7-naphthalen-2-yl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.000025
-
7-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-6-yn-1-one
-
-
0.0000047
-
7-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.0000008
-
7-phenyl-1-[5-(trifluoromethyl)-1,3-oxazol-2-yl]heptan-1-one
-
-
0.00004
-
7-phenyl-1-[5-[6-(1H-tetrazol-5-yl)pyridin-2-yl]-1,3-oxazol-2-yl]heptan-1-one
-
-
0.00028
-
7-pyridin-2-yl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-6-yn-1-one
-
-
0.00012
-
7-pyridin-2-yl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.0003
-
7-pyridin-3-yl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-6-yn-1-one
-
-
0.000032
-
7-pyridin-3-yl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.00015
-
7-pyridin-4-yl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-6-yn-1-one
-
-
0.0000033
-
7-[2-(methylsulfanyl)phenyl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.0000042
-
7-[3-(methylsulfanyl)phenyl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.0000024
-
7-[3-(tert-butylamino)phenyl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.0000025
-
7-[4-(methylsulfanyl)phenyl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.0000056
-
7-[4-(tert-butylamino)phenyl]-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
-
-
0.0000075
-
8-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)octan-1-one
-
-
0.000008
-
9-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)nonan-1-one
-
-
0.005
-
acetaldehyde
-
strain AI3
0.0051
-
acetaldehyde
-
competitive inhibitor
0.007
-
acetaldehyde ammonia
-
strain AI3
12.1
-
acetamide
-
at pH 7 and 50C; pH 7.2
0.0014
-
acetanilide
-
substrate 4-nitroacetanilide, competitive inhibition
120
-
acetonnitrile
-
-
2.3
-
Acrylamide
-
at pH 7 and 50C; pH 7.2
34
-
acrylonitrile
-
-
2
-
Al3+
-
pH 9.0, 37C
9.8
-
Ca2+
-
pH 9.0, 37C
0.001
-
Chloroacetone
-
pH 7.1, 25C, irreversible
1.1
-
Cyanate
-
strain AI3, pH 7.2 and 37C
1.8
-
Cyanate
-
strain AIU1N, pH 7.2 and 37C
50
-
Cyanate
-
strain OUCH4, pH 7.2 and 37C
0.0008
-
cyclopentyl(5-pyridin-2-yl-1,3-oxazol-2-yl)methanone
-
-
0.0025
-
cyclopropyl(5-pyridin-2-yl-1,3-oxazol-2-yl)methanone
-
-
0.000091
-
ethyl 4-[3-(4,5-diphenyl-1H-imidazol-1-yl)phenoxy]butanoate
-
-
0.000064
-
ethyl 4-[4-(4,5-diphenyl-1H-imidazol-1-yl)phenoxy]butanoate
-
-
0.000032
-
ethyl 6-(2-ethyl-4,5-diphenyl-1H-imidazol-1-yl)hexanoate
-
-
0.000003
-
ethyl 6-[2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazol-5-yl]pyridine-2-carboxylate
-
-
0.000011
-
ethyl 7-(2-ethyl-4,5-diphenyl-1H-imidazol-1-yl)heptanoate
-
-
0.000064
-
ethyl 7-[4,5-bis(4-fluorophenyl)-2-methyl-1H-imidazol-1-yl]heptanoate
-
-
0.000031
-
ethyl 8-(2-ethyl-4,5-diphenyl-1H-imidazol-1-yl)octanoate
-
-
1.4
-
Hydroxyurea
-
strain AI3, pH 7.2 and 37C
10.8
-
Hydroxyurea
-
strain AIU1N, pH 7.2 and 37C
337
-
Hydroxyurea
-
strain OUCH4, pH 7.2 and 37C
67
-
K+
-
pH 9.0, 37C
90
-
Methacrylonitrile
-
-
0.00000026
-
methyl 2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazole-5-carboxylate
-
-
0.0000015
-
methyl 2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazole-5-carboxylate
-
-
0.0000012
-
methyl 2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazole-5-sulfinate
-
-
0.0000009
-
methyl 2-(7-phenylheptanoyl)-1,3-oxazole-5-carboxylate
-
-
0.0000017
-
methyl 2-[3-(4-phenoxyphenyl)propanoyl]-1,3-oxazole-5-carboxylate
-
-
0.0000016
-
methyl 2-[3-[4-(phenoxymethyl)phenyl]propanoyl]-1,3-oxazole-5-carboxylate
-
-
0.000034
-
methyl 2-[7-oxo-7-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-1-yn-1-yl]benzoate
-
-
0.000037
-
methyl 2-[7-oxo-7-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptyl]benzenesulfinate
-
-
0.000001
-
methyl 2-[7-oxo-7-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptyl]benzoate
-
-
0.00003
-
methyl 3-[7-oxo-7-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-1-yn-1-yl]benzoate
-
-
0.0000013
-
methyl 3-[7-oxo-7-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptyl]benzenesulfinate
-
-
0.0000019
-
methyl 3-[7-oxo-7-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptyl]benzoate
-
-
0.0000015
-
methyl 4-[2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazol-5-yl]pyridine-2-carboxylate
-
-
0.000025
-
methyl 4-[7-oxo-7-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-1-yn-1-yl]benzoate
-
-
0.000019
-
methyl 4-[7-oxo-7-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptyl]benzenesulfinate
-
-
0.0000015
-
methyl 4-[7-oxo-7-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptyl]benzoate
-
-
0.007
-
methyl 5-oxo-5-(5-pyridin-2-yl-1,3-oxazol-2-yl)pentanoate
-
-
0.0000006
-
methyl 5-[2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazol-5-yl]pyridine-2-carboxylate
-
-
0.000031
-
methyl 6-(2-[3-[4'-(morpholin-4-ylmethyl)biphenyl-4-yl]propyl]-1,3-oxazol-5-yl)pyridine-2-carboxylate
-
-
0.000009
-
methyl 6-(2-[3-[4'-(piperidin-1-ylmethyl)biphenyl-4-yl]propyl]-1,3-oxazol-5-yl)pyridine-2-carboxylate
-
-
0.000018
-
methyl 6-(2-[3-[4'-(pyrrolidin-1-ylmethyl)biphenyl-4-yl]propyl]-1,3-oxazol-5-yl)pyridine-2-carboxylate
-
-
0.000005
-
methyl 6-(2-[3-[4'-(thiomorpholin-4-ylmethyl)biphenyl-4-yl]propyl]-1,3-oxazol-5-yl)pyridine-2-carboxylate
-
-
0.000002
-
methyl 6-(2-[3-[4-(phenoxymethyl)phenyl]propanoyl]-1,3-oxazol-5-yl)pyridine-2-carboxylate
-
-
0.0000035
-
methyl 6-(2-[3-[4-(phenoxymethyl)phenyl]propanoyl]-1,3-oxazol-5-yl)pyridine-3-carboxylate
-
-
0.0045
-
methyl 6-(2-[3-[4-(piperidin-4-yloxy)phenyl]propyl]-1,3-oxazol-5-yl)pyridine-2-carboxylate
-
-
0.0018
-
methyl 6-oxo-6-(5-pyridin-2-yl-1,3-oxazol-2-yl)hexanoate
-
-
0.009
-
methyl 6-[2-(3-biphenyl-4-yl-1-hydroxypropyl)-1,3-oxazol-5-yl]pyridine-2-carboxylate
-
-
0.0011
-
methyl 6-[2-(3-biphenyl-4-yl-1-hydroxypropyl)-1,3-oxazol-5-yl]pyridine-3-carboxylate
-
-
0.0000012
-
methyl 6-[2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazol-5-yl]pyridine-2-carboxylate
-
-
0.0000022
-
methyl 6-[2-(3-biphenyl-4-ylpropanoyl)-1,3-oxazol-5-yl]pyridine-3-carboxylate
-
-
0.007
-
methyl 6-[2-(3-biphenyl-4-ylpropyl)-1,3-oxazol-5-yl]pyridine-2-carboxylate
-
-
0.05
-
methyl 6-[2-(3-biphenyl-4-ylpropyl)-1,3-oxazol-5-yl]pyridine-3-carboxylate
-
-
0.000029
-
methyl 6-[2-(3-[4'-[(dimethylamino)methyl]biphenyl-4-yl]propyl)-1,3-oxazol-5-yl]pyridine-2-carboxylate
-
-
0.000008
-
methyl 6-[2-(7-phenylheptanoyl)-1,3-oxazol-5-yl]pyridine-2-carboxylate
-
-
0.0000035
-
methyl 6-[2-(7-phenylheptanoyl)-1,3-oxazol-5-yl]pyridine-3-carboxylate
-
-
0.000013
-
methyl 6-[2-[3-(4-phenoxyphenyl)propanoyl]-1,3-oxazol-5-yl]pyridine-2-carboxylate
-
-
0.000054
-
methyl 6-[2-[3-(4-phenoxyphenyl)propanoyl]-1,3-oxazol-5-yl]pyridine-3-carboxylate
-
-
0.000032
-
methyl 6-[2-[3-(4-[[1-(tert-butoxycarbonyl)piperidin-4-yl]oxy]phenyl)propyl]-1,3-oxazol-5-yl]pyridine-2-carboxylate
-
-
0.0065
-
methyl [3-(4,5-diphenyl-1H-imidazol-1-yl)phenoxy]acetate
-
-
0.00067
-
methyl [4-(4,5-diphenyl-1H-imidazol-1-yl)phenoxy]acetate
-
-
0.000002
-
N,N-dimethyl-2-(7-phenylheptanoyl)-1,3-oxazole-5-carboxamide
-
-
0.0000054
-
N,N-dimethyl-2-[3-(4-phenoxyphenyl)propanoyl]-1,3-oxazole-5-carboxamide
-
-
0.0000067
-
N,N-dimethyl-2-[3-[4-(phenoxymethyl)phenyl]propanoyl]-1,3-oxazole-5-carboxamide
-
-
0.00097
-
N,N-dimethyl-7-(2-methyl-4,5-diphenyl-1H-imidazol-1-yl)heptanamide
-
-
0.02
-
N-benzyl-5-oxo-5-(5-pyridin-2-yl-1,3-oxazol-2-yl)pentanamide
-
-
0.000007
-
N-methyl-2-(7-phenylheptanoyl)-1,3-oxazole-5-carboxamide
-
-
0.000011
-
N-methyl-2-[3-(4-phenoxyphenyl)propanoyl]-1,3-oxazole-5-carboxamide
-
-
0.0000036
-
N-methyl-2-[3-[4-(phenoxymethyl)phenyl]propanoyl]-1,3-oxazole-5-carboxamide
-
-
0.0017
-
N-methyl-7-(2-methyl-4,5-diphenyl-1H-imidazol-1-yl)heptanamide
-
-
22
-
Na+
-
pH 9.0, 37C
0.0000089
-
Phenacetin
-
substrate 4-nitroacetanilide, competitive inhibition, induction of activity for 2-nitrophenylacetate and methylbutyrate
0.0000016
-
phenyl [6-(2-methyl-4,5-diphenyl-1H-imidazol-1-yl)hexyl]carbamate
-
-
280
-
Propionitrile
-
-
0.000012
-
tert-butyl [3-[7-oxo-7-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-1-yn-1-yl]phenyl]carbamate
-
-
0.000023
-
tert-butyl [4-[7-oxo-7-(5-pyridin-2-yl-1,3-oxazol-2-yl)hept-1-yn-1-yl]phenyl]carbamate
-
-
6.6
-
Urea
-
strain AI3, pH 7.2 and 37C
41.3
-
Urea
-
strain AIU1N pH 7.2 and 37C
4.9
-
Mg2+
-
pH 9.0, 37C
additional information
-
additional information
-
inhibition kinetics
-
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.09
-
(+/-)-(5Z,8Z,11Z,14Z)-N-((2,2-dimethyl-1,3-dioxolan-4-yl)methyl)icosa-5,8,11,14-tetraenamide
-
-
0.098
-
(+/-)-2,2-dimethyl-1,3-dioxolan-4-ylmethyl (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate
-
-
0.012
-
(+/-)-oxiran-2-ylmethyl (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate
-
-
0.0082
-
(+/-)-oxiran-2-ylmethyl (9Z)-hexadec-9-enoate
-
-
0.011
-
(+/-)-oxiran-2-ylmethyl (9Z)-octadec-9-enoate
-
-
0.01
-
(+/-)-oxiran-2-ylmethyl (9Z,12Z)-octadeca-9,12-dienoate
-
-
0.0016
-
(+/-)-oxiran-2-ylmethyl (9Z,12Z,15Z)-octadeca-9,12,15-trienoate
-
-
0.061
-
(+/-)-oxiran-2-ylmethyl 1,1'-biphenyl-3-carboxylate
-
-
0.035
-
(+/-)-oxiran-2-ylmethyl 1,1'-biphenyl-4-carboxylate
-
-
0.051
-
(+/-)-tetrahydro-2H-pyran-2-ylmethyl (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate
-
-
0.01
-
(+/-)-tetrahydro-2H-pyran-2-ylmethyl (9Z)-hexadec-9-enoate
-
-
0.029
-
(+/-)-tetrahydro-2H-pyran-2-ylmethyl (9Z)-octadec-9-enoate
-
-
0.0075
-
(+/-)-tetrahydro-2H-pyran-2-ylmethyl (9Z,12Z)-octadeca-9,12-dienoate
-
-
0.019
-
(+/-)-tetrahydro-2H-pyran-2-ylmethyl (9Z,12Z,15Z)-octadeca-9,12,15-trienoate
-
-
0.043
-
(+/-)-tetrahydro-2H-pyran-2-ylmethyl 1,1'-biphenyl-2-carboxylate
-
-
0.018
-
(+/-)-tetrahydro-2H-pyran-2-ylmethyl 1,1'-biphenyl-3-carboxylate
-
-
0.032
-
(+/-)-tetrahydro-2H-pyran-2-ylmethyl 1,1'-biphenyl-4-carboxylate
-
-
0.053
-
(+/-)-tetrahydro-2H-pyran-2-ylmethyl benzoate
-
-
0.0049
-
(2R,3R)-allo-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester
-
pH 5.0, 37C, recombinant enzyme
0.0049
-
(2R,3R)-allo-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester
-
pH 5.0, 37C, recombinant enzyme
0.1
-
(2R,3S)-2-methyl-4-oxo-3-oxetanylcarbamic acid benzyl ester
-
pH 5.0, 37C, recombinant enzyme
0.1
-
(2R,3S)-2-methyl-4-oxo-3-oxetanylcarbamic acid benzyl ester
-
pH 5.0, 37C, recombinant enzyme
0.08
-
(2R,3S)-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester
-
pH 5.0, 37C, recombinant enzyme
0.08
-
(2R,3S)-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester
-
pH 5.0, 37C, recombinant enzyme
0.000127
-
(2S,3R)-2-methyl-4-oxo-3-oxetanylcarbamic acid 5-phenylpentyl ester
-
pH 5.0, 37C, recombinant enzyme
0.000127
-
(2S,3R)-2-methyl-4-oxo-3-oxetanylcarbamic acid 5-phenylpentyl ester
-
pH 5.0, 37C, recombinant enzyme
0.001
-
(2S,3R)-2-methyl-4-oxo-3-oxetanylcarbamic acid benzyl ester
-
pH 5.0, 37C, recombinant enzyme
0.001
-
(2S,3R)-2-methyl-4-oxo-3-oxetanylcarbamic acid benzyl ester
-
pH 5.0, 37C, recombinant enzyme
0.00022
-
(2S,3R)-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester
-
pH 5.0, 37C, recombinant enzyme
0.00022
-
(2S,3R)-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester
-
pH 5.0, 37C, recombinant enzyme
0.00019
-
(2S,3S)-allo-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester
-
pH 5.0, 37C, recombinant enzyme
0.00019
-
(2S,3S)-allo-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester
-
pH 5.0, 37C, recombinant enzyme
0.01
-
(5Z,8Z,11Z,14Z)-eicosantetraenoic acid 3-thienyl methyl ester
-
i.e. CAY-10402, IC50: 0.010 mM, 37% inhibition at 0.1 mM
0.0005
-
(R)-(2-oxo-3-oxetanyl)carbamic acid tert-butyl ester
-
pH 5.0, 37C, recombinant enzyme
0.0005
-
(R)-(2-oxo-3-oxetanyl)carbamic acid tert-butyl ester
-
pH 5.0, 37C, recombinant enzyme
0.0492
-
(R)-1-methyl-3-(2-oxo-3-oxetanyl)urea
-
pH 5.0, 37C, recombinant enzyme
0.0492
-
(R)-1-methyl-3-(2-oxo-3-oxetanyl)urea
-
pH 5.0, 37C, recombinant enzyme
0.0007
-
(R)-2-oxo-3-oxetanylcarbamic acid benzyl ester
-
pH 5.0, 37C, recombinant enzyme
0.0007
-
(R)-2-oxo-3-oxetanylcarbamic acid benzyl ester
-
pH 5.0, 37C, recombinant enzyme
0.1
-
(R,S)-2-oxocyclobutylcarbamic acid benzyl ester
-
pH 5.0, 37C, recombinant enzyme
0.1
-
(R,S)-2-oxocyclobutylcarbamic acid benzyl ester
-
pH 5.0, 37C, recombinant enzyme
0.00058
-
(S)-(2-oxo-3-oxetanyl)carbamic acid tert-butyl ester
-
pH 5.0, 37C, recombinant enzyme
0.00058
-
(S)-(2-oxo-3-oxetanyl)carbamic acid tert-butyl ester
-
pH 5.0, 37C, recombinant enzyme
0.015
-
(S)-1-methyl-3-(2-oxo-3-oxetanyl)urea
-
pH 5.0, 37C, recombinant enzyme
0.015
-
(S)-1-methyl-3-(2-oxo-3-oxetanyl)urea
-
pH 5.0, 37C, recombinant enzyme
0.00296
-
(S)-2-oxo-3-oxetanylcarbamic acid benzyl ester
-
pH 5.0, 37C, recombinant enzyme
0.00296
-
(S)-2-oxo-3-oxetanylcarbamic acid benzyl ester
-
pH 5.0, 37C, recombinant enzyme
0.091
-
1,3-benzodioxol-5-ylmethyl (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate
-
-
0.0057
-
1-(1,3-oxazol-2-yl)-4-piperidin-4-ylbutan-1-one
-
-
0.000022
-
1-(1,3-oxazol-2-yl)-4-[1-(3-phenylpropanoyl)piperidin-4-yl]butan-1-one
-
-
0.0005
-
1-(1,3-oxazol-2-yl)-4-[1-(phenylacetyl)piperidin-4-yl]butan-1-one
-
-
0.0000025
-
1-(1,3-oxazol-2-yl)-4-[1-(phenylsulfonyl)piperidin-4-yl]butan-1-one
-
-
0.000006
-
1-(1,3-oxazol-2-yl)-4-[1-[(2-phenylethyl)sulfonyl]piperidin-4-yl]butan-1-one
-
-
0.0062
-
1-arachidonoylglycerol
-
IC50: 0.0062 mM
0.018
-
1-myristin
-
IC50: 0.018 mM, complete inhibition is possible
0.008
-
1-palmitin
-
IC50: 0.0080 mM, complete inhibition is possible
0.01
-
2,3-dihydroxypropyl (11Z)-icos-11-enoate
-
i.e. O-4066, IC50: 0.010 mM, complete inhibition is possible
0.0057
-
2,3-dihydroxypropyl (11Z,14Z)-icosa-11,14-dienoate
-
i.e. O-3907, IC50: 0.0057 mM, complete inhibition is possible
0.011
-
2,3-dihydroxypropyl (5Z)-icos-5-enoate
-
i.e. O-3908, IC50: 0.011 mM, 84% inhibition is possible
0.0024
-
2,3-dihydroxypropyl (5Z,8Z,11Z)-2-ethylcycloheptadeca-5,8,11-triene-1-carboxylate
-
i.e. O-3973, IC50: 0.0024, 95% inhibition is possible
0.0076
-
2,3-dihydroxypropyl (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoate
-
i.e. O-3832, IC50: 0.0076 mM, complete inhibition is possible
0.0051
-
2,3-dihydroxypropyl (6Z,9Z,12Z,15Z)-cyclohenicosa-6,9,12,15-tetraene-1-carboxylate
-
i.e. O-4081, IC50: 0.0051 mM, 90% inhibition is possible
0.014
-
2,3-dihydroxypropyl (7Z,10Z,13Z,16Z)-docosa-7,10,13,16-tetraenoate
-
i.e. O-3872, IC50: 0.014, complete inhibition is possible
0.023
-
2,3-dihydroxypropyl (8Z,11Z,14Z)-icosa-8,11,14-trienoate
-
i.e. 3846, IC50: 0.023, complete inhibition is possible
0.021
-
2-(5,5-dimethyl-1,3-dioxan-2-yl)ethyl (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate
-
-
0.000002
-
2-fluorophenyl [6-(2-methyl-4,5-diphenyl-1H-imidazol-1-yl)hexyl]carbamate
-
-
0.00000038
-
3'-carbamoylbiphenyl-3-yl undec-10-yn-1-ylcarbamate
O00519, Q6GMR7
-
0.000001
-
3'-carbamoylbiphenyl-3-yl undec-10-yn-1-ylcarbamate
O00519, Q6GMR7
-
0.000077
-
4-(1-benzoylpiperidin-4-yl)-1-(1,3-oxazol-2-yl)butan-1-one
-
-
0.000315
-
4-(1-benzylpiperidin-4-yl)-1-(1,3-oxazol-2-yl)butan-1-one
-
-
0.000283
-
4-(benzyloxy)phenyl butylcarbamate
O00519, Q6GMR7
-
0.00136
-
4-(benzyloxy)phenyl butylcarbamate
O00519, Q6GMR7
-
0.000315
-
4-[1-(2-bromobenzyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
0.00026
-
4-[1-(2-chlorobenzyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
0.004
-
4-[1-(2-methoxybenzyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
0.0012
-
4-[1-(2-methylbenzyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
0.00009
-
4-[1-(3,3-dimethylbutanoyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
0.00001
-
4-[1-(3-bromobenzyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
0.00006
-
4-[1-(3-chlorobenzyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
0.000003
-
4-[1-(3-cyclohexylpropanoyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
0.000034
-
4-[1-(3-methoxybenzyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
0.00009
-
4-[1-(3-methylbenzyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
0.000045
-
4-[1-(4-bromobenzyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
0.000066
-
4-[1-(4-chlorobenzyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
0.00013
-
4-[1-(4-methoxybenzyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
0.0004
-
4-[1-(4-methylbenzyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
0.0006
-
4-[1-(benzylsulfonyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
0.0001
-
4-[1-(cyclohexylacetyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
0.000045
-
4-[1-(cyclohexylcarbonyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
0.00025
-
4-[1-(cyclopropylcarbonyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
0.00014
-
4-[1-(isopropylsulfonyl)piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
0.000005
-
4-[1-[3-(1-methylethoxy)benzyl]piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
0.000001
-
4-[1-[3-(1-methylethyl)benzyl]piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
0.000014
-
4-[1-[4-(1-methylethoxy)benzyl]piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
0.0000036
-
4-[1-[4-(1-methylethyl)benzyl]piperidin-4-yl]-1-(1,3-oxazol-2-yl)butan-1-one
-
-
0.0000134
-
7-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
O00519, Q6GMR7
-
0.000206
-
7-phenyl-1-(5-pyridin-2-yl-1,3-oxazol-2-yl)heptan-1-one
O00519, Q6GMR7
-
0.033
-
alpha-methyl-1-arachidonoylglycerol
-
IC50: 0.033 mM, complete inhibition at 0.1 mM
0.058
-
arachidonic acid
-
IC50: 0.058 mM, complete inhibition at 0.1 mM
0.0049
-
arachidonoyl glycine
-
IC50: 0.0049 mM, complete inhibition at 0.1 mM
0.078
-
arachidonoyl serinol
-
IC50: 0.078 mM, complete inhibition at 0.1 mM
0.000004
-
benzyl 4-[4-(1,3-oxazol-2-yl)-4-oxobutyl]piperidine-1-carboxylate
-
-
0.0000005
-
CAY10400
-
-
0.1
-
cyclobutylcarbamic acid benzyl ester
-
pH 5.0, 37C, recombinant enzyme
0.1
-
cyclobutylcarbamic acid benzyl ester
-
pH 5.0, 37C, recombinant enzyme
0.000004
-
isopropyl 4-[4-(1,3-oxazol-2-yl)-4-oxobutyl]piperidine-1-carboxylate
-
-
0.0026
-
N-(4-hydroxy-2-methylphenyl)arachidonylamide
-
i.e. VDM11, an anandamide uptake inhibitor, IC50 is 0.0026 mM, inhibitory potency depends upon the concentration of bovine serum albumin
0.0021
-
N-(4-hydroxyphenyl)arachidonylamide
-
i.e. AM404, an anandamide uptake inhibitor, IC50 is 0.0021 mM
0.000115
-
N-[(3S)-2-oxooxetan-3-yl]biphenyl-4-carboxamide
-
pH 5.0, 37C, recombinant enzyme
0.000115
-
N-[(3S)-2-oxooxetan-3-yl]biphenyl-4-carboxamide
-
pH 5.0, 37C, recombinant enzyme
0.1
-
N-[(benzyloxy)carbonyl]-D-serine
-
pH 5.0, 37C, recombinant enzyme
0.1
-
N-[(benzyloxy)carbonyl]-D-serine
-
pH 5.0, 37C, recombinant enzyme
0.1
-
N-[(benzyloxy)carbonyl]-L-serine
-
pH 5.0, 37C, recombinant enzyme
0.1
-
N-[(benzyloxy)carbonyl]-L-serine
-
pH 5.0, 37C, recombinant enzyme
0.003
-
noladin ether
-
IC50: 0.003 mM, complete inhibition at 0.1 mM
0.000002
-
tert-butyl 4-[4-(1,3-oxazol-2-yl)-4-oxobutyl]piperidine-1-carboxylate
-
-
0.0053
-
tetrahydro-2H-pyran-4-yl (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate
-
-
0.05
-
UCM707
-
-
0.000002
-
URB597
-
-
0.005
-
VDM11
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.000028
-
-
substrate indoleacetonitrile at 30C, activity after purification
0.00003
-
-
growth substrate 2-monochloropropionic acid, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PP3
0.000031
-
-
substrate indole-3-acetamide at 30C, activity after purification
0.00024
-
-
growth substrate succinate, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PP3
0.00027
-
-
growth substrate 2-chloropropionamide, 2-chloropropionamide-amidase activity, measured with 14 mM 2-chloropropionamide, strain PP3
0.00074
-
-
membrane fraction
0.0017
-
-
growth substrate propionamide, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PAU3
0.0021
-
-
D191E mutant, supernatant of cell-free extract, 0.75% of wild-type activity
0.00243
-
-
harboring pALJ20, substrate cyclohexanecarboxamide with cobalt
0.004
-
Q70I53
acetylspermidine, pH 7.5
0.008
-
Q70I53
acetylcadaverine, pH 7.5
0.011
-
Q70I53
acetylputrescine, pH 7.5
0.0135
-
-
substrate cyclohexanecarboxamide with cobalt
0.019
-
-
growth substrate succinate, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PPW3
0.023
-
-
growth substrate 2-monochloropropionic acid, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PPW3
0.0306
-
-
C203A mutant, supernatant of cell-free extract, 10.8% of wild-type activity
0.031
-
-
growth substrate succinate, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PAU5
0.039
-
-
growth substrate propionamide, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PAU5
0.05
-
-
substrate acetamide, crude extract
0.078
-
-
A-amidase, substrate was 2-chloropropionamide
0.12
-
-
growth substrate 2-chloropropionamide, 2-chloropropionamide-amidase activity, measured with 14 mM 2-chloropropionamide, strain PPW3
0.14
-
-
growth substrate 2-monochloropropionic acid, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PAU5
0.162
-
-
D191E mutant, purified, 1.33% of purified wild-type amidase
0.18
-
-
substrate urea
0.19
-
-
>99% enantiomeric excess
0.2
-
-
strain N6-836, formamide, pH 7.4, room temperature, 30 min; strain N6-ureB::TnKm-4, formamide, pH 7.4, room temperature, 30 min
0.23
-
-
strain N6, isobutyramide, pH 7.4, room temperature, 30 min
0.25
-
-
growth substrate 2-chloropropionamide, 2-chloropropionamide-amidase activity, measured with 14 mM 2-chloropropionamide, strain PAU5
0.25
-
-
strain N6, formamide, pH 7.4, room temperature, 30 min
0.283
-
-
wild-type amidase
0.3
-
-
substrate valeramide, strain R312
0.35
-
-
strain N6-ureB::TnKm-4, isobutyramide, pH 7.4, room temperature, 30 min
0.383
-
-
strain JM 109 harboring pKPO2(6)
0.468
-
-
harboring pALJ30, substrate cyclohexanecarboxamide with cobalt
0.6
-
-
substrate pivalamide, strain R312
0.67
-
-
activity in cell extract, substrate 1 mM phenylacetamide
0.8
-
-
at 3 mM formamide, formamidase activity of AI3 amidase
0.8
-
-
substrate benzamide, strain R312
0.81
-
-
activity in cell extract, substrate 1 mM ketoprofen amide
0.89
-
-
strain B6, substrate para-nitroacetanilide, pH 7.2, 25C
0.91
-
-
strain L10, substrate para-nitroacetanilide, pH 7.2, 25C
0.914
-
-
strain PPW, activity of C-amidase after preparative PAGE, final yield 17% of original activity
1.18
-
-
85C, pH not specified in the publication, substrate: benzamide
1.3
-
-
activity in cell extract, substrate 1 mM naproxen amide
1.4
-
-
C203Amutant, purified, 11.5% of purified wild-type amidase
1.5
-
-
4-nitroacetanilide, strain OUCH 4, pH 8.5; substrate 4-nitro-acetanilide, strain OUCH4
1.51
-
-
mutant strain MM15 measured with acrylamide as substrate, growth conditions is continuous
1.89
-
-
strain PPW3, C-amidase, substrate 2-chloropropionamide
2.1
-
-
mutant strain MM15 measured with acetamide as substrate
2.35
-
-
strain OUCH, substrate para-nitroacetanilide, pH 7.2, 25C
3.5
-
-, Q5YFS2
purified recombinant enzyme, substrate glutaryl-nitro-5-amino benzoic acid
3.59
-
-
mutant strain MM15 measured with acrylamide as substrate, growth conditions is fed-batch
4.1
-
-
activity after purification, substrate 5 mM phenylacetamide
4.67
-
-, Q5YFS2
purified recombinant enzyme, substrate adipoyl-nitro-5-amino benzoic acid
4.83
-
-
substrate methacrylamide
4.89
-
-
strain AIU1N, substrate para-nitroacetanilide, pH 7.2, 25C
6.5
-
-
strain N6, acetamide, pH 7.4, room temperature, 30 min
6.69
-
-
substrate cyclohexanamide
7.4
-
-
4-nitroacetanilide, strain AIU 1N, pH 8.5; substrate 4-nitro-acetanilide, strain AIU1N
7.7
-
-
substrate 4-nitroacetanilide, crude extract
8.04
-
-
strain AI3, substrate para-nitroacetanilide, pH 7.2, 25C
9
-
-
substrate acetamide, strain R312; substrate isobutyramide, strain R312
10
-
-
purified enzyme, pH 7.5, 30C
10.5
-
-
4-nitroacetanilide, strain AI3, pH 8.5; substrate 4-nitro-acetanilide, strain AI3 25-fold greater activity than wild-type
11
-
-
strain N6, acrylamide, pH 7.4, room temperature, 30 min
11.6
-
-
wild-type amidase after purification
12
-
-
strain N6, propionamide, pH 7.4, room temperature, 30 min
12.2
-
-
substrate benzamide; transformant, substrate benzamide
12.2
-
-
purified from a soluble fraction; wild-type amidase, purified
14.8
-
-
purified recombinant His6-tagged enzyme
16
-
-
substrate DL-lactamide, strain R312
16
-
-
purified recombinant wild-type enzyme
16.39
-
-
substrate hexanamide
16.5
-
-
specific activity of S(+)-2-phenylpropionic acid
16.7
-
-
substrate propionamide, strain R312
17
-
-
strain N6-ureB::TnKm-4, acetamide, pH 7.4, room temperature, 30 min
17.7
-
-
turnover propionamide to propionate and ammonia
19.4
-
-
purified enzyme, substrate benzamide
23.5
-
-
purified enzyme, substrate methacrylamide
25.4
-
-
purified enzyme, substrate propionamide
26.8
-
-
purified enzyme, substrate acrylamide
28
-
-
strain N6-ureB::TnKm-4, acrylamide, pH 7.4, room temperature, 30 min; strain N6-ureB::TnKm-4, propionamide, pH 7.4, room temperature, 30 min
37.5
-
-
reombinant enzyme in crude extract, at pH 7 and 50C
37.6
-
-
micromol of NH3 * min-1 * mg of protein-1
50
-