Information on EC 3.5.1.4 - amidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.5.1.4
-
RECOMMENDED NAME
GeneOntology No.
amidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a monocarboxylic acid amide + H2O = a monocarboxylate + NH3
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxylic acid amide hydrolysis
-
-
hydrolysis of amide bond
-
-
hydrolysis of carboxylic acid amide
transfer of acyl group
transfer of amide group
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
acrylonitrile degradation I
-
-
Aminobenzoate degradation
-
-
Arginine and proline metabolism
-
-
arginine metabolism
-
-
degradation of aromatic, nitrogen containing compounds
-
-
IAA biosynthesis
-
-
indole-3-acetate biosynthesis II
-
-
indole-3-acetate biosynthesis III (bacteria)
-
-
indole-3-acetate biosynthesis IV (bacteria)
-
-
L-arginine degradation X (arginine monooxygenase pathway)
-
-
Microbial metabolism in diverse environments
-
-
Phenylalanine metabolism
-
-
Styrene degradation
-
-
Tryptophan metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
acylamide amidohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9012-56-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene ana
SwissProt
Manually annotated by BRENDA team
Aeribacillus pallidus BTP-5x MTCC 9225
isolated from thermal springs of Tatapani (Himachal Pradesh)
-
-
Manually annotated by BRENDA team
Crown gall
-
-
Manually annotated by BRENDA team
strain FB188, or Bordetella sp.
SwissProt
Manually annotated by BRENDA team
Alcaligenes sp. MTCC 10674
isolated as a nitrile degrading bacterium from the soil
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Blastobacter sp.
strain A17p-4
-
-
Manually annotated by BRENDA team
strain ZJB-07021
-
-
Manually annotated by BRENDA team
strain ZJB-07021
-
-
Manually annotated by BRENDA team
strain R312
-
-
Manually annotated by BRENDA team
strain AM10-C-1
-
-
Manually annotated by BRENDA team
strain AM10-C-1
-
-
Manually annotated by BRENDA team
strain C, hospital isolate
-
-
Manually annotated by BRENDA team
strain C, hospital isolate
-
-
Manually annotated by BRENDA team
an acrylamide-degrading bacterium isolated from the grounds of a palm estate in Serdang, Selangor; previously isolated from the grounds of a palm estate in Serdang, Selangor
-
-
Manually annotated by BRENDA team
an acrylamide-degrading bacterium isolated from the grounds of a palm estate in Serdang, Selangor; previously isolated from the grounds of a palm estate in Serdang, Selangor
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Duch, tissues of aseptically grown squash
-
-
Manually annotated by BRENDA team
strain AE1
-
-
Manually annotated by BRENDA team
strain KPO-2771-4
-
-
Manually annotated by BRENDA team
strain MA52
-
-
Manually annotated by BRENDA team
strain MA52
-
-
Manually annotated by BRENDA team
strain JM109 harboring pALJ30 and pALJ20
-
-
Manually annotated by BRENDA team
strain RB791 and HB101
-
-
Manually annotated by BRENDA team
isolated from Manikaran thermal spring (Himachal Pradesh, India)
-
-
Manually annotated by BRENDA team
isolated from Manikaran thermal spring (Himachal Pradesh, India)
-
-
Manually annotated by BRENDA team
i.e. HIV1
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
wild-type is strain MM6, mutant strains are MM8 and MM15
-
-
Manually annotated by BRENDA team
strain CBS 498-74
-
-
Manually annotated by BRENDA team
strain CBS 498-74
-
-
Manually annotated by BRENDA team
Nesterenkonia sp.
isolated from mineral soil collected from the Miers Valley, McMurdo region, eastern Antarctica
UniProt
Manually annotated by BRENDA team
isolated from mineral soil collected from the Miers Valley, McMurdo region, eastern Antarctica
UniProt
Manually annotated by BRENDA team
strain T12
-
-
Manually annotated by BRENDA team
strain T12
-
-
Manually annotated by BRENDA team
gene pamH
UniProt
Manually annotated by BRENDA team
strain OMGS 100 and H66
-
-
Manually annotated by BRENDA team
strain OMGS 100 and H66
-
-
Manually annotated by BRENDA team
strain AI3, AIU1N and OUCH4; strain AI3 and the mutant strains AIU1N and OUCH4
-
-
Manually annotated by BRENDA team
Pseudomonas aeruginosa PAC 111 (C11)
strain PAC 111 (C11)
-
-
Manually annotated by BRENDA team
Pseudomonas aeruginosa PAC 142 (L10R)
strain PAC 142 (L10R)
-
-
Manually annotated by BRENDA team
PAC142
-
-
Manually annotated by BRENDA team
strain B23
-
-
Manually annotated by BRENDA team
strain B23
-
-
Manually annotated by BRENDA team
strain 5B
-
-
Manually annotated by BRENDA team
isolated from rhizosphere of cyanogenic plant Circium vulgare
-
-
Manually annotated by BRENDA team
strain PP3 and PPW3
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-
Manually annotated by BRENDA team
strain W13
-
-
Manually annotated by BRENDA team
thermophilic actinomycete, DSMZ 43832
-
-
Manually annotated by BRENDA team
DSM 9174
-
-
Manually annotated by BRENDA team
strain MP50
-
-
Manually annotated by BRENDA team
sequence identical to the sequenced enzyme fragment from strain NCIMB11540; NCIMB11540
SwissProt
Manually annotated by BRENDA team
Rhodococcus erythropolis No. 7
strain No. 7
UniProt
Manually annotated by BRENDA team
strain IFO 15564
-
-
Manually annotated by BRENDA team
strain J1
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-
Manually annotated by BRENDA team
strain AA21
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-
Manually annotated by BRENDA team
strain R312
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-
Manually annotated by BRENDA team
strain S13-4
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-
Manually annotated by BRENDA team
bacteriophage isolated from Pneumococcus sp., strain 101/87
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-
Manually annotated by BRENDA team
wild-type strain R6
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-
Manually annotated by BRENDA team
gene azl13
UniProt
Manually annotated by BRENDA team
gene azl13
UniProt
Manually annotated by BRENDA team
strain 7, gene ST0478
-
-
Manually annotated by BRENDA team
strain 7, gene ST0478
-
-
Manually annotated by BRENDA team
Torula cremoris
-
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
metabolism
RhAmidase belongs to amidase signature family, a group of amidase families, and is responsible for the degradation of amides produced from nitriles by nitrile hydratase
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-(+)-2-chloropropionamide + H2O
(+)-2-chloropropionic acid + NH3
show the reaction diagram
(R)-2,2-dimethyl cyclopropanecarboxamide + H2O
?
show the reaction diagram
(R)-2,2-dimethyl cyclopropanecarboxamide + H2O
propanecarboxylic acid + NH3
show the reaction diagram
(R)-2-chloromandelamide + H2O
(R)-2-chloromandelic acid + NH3
show the reaction diagram
(R,S)-2-(3-benzoylphenyl)propanamide + H2O
(S)-2-(3-benzoylphenyl)propanoic acid + NH3
show the reaction diagram
-
enantioselective for the S-enantiomer
-
-
?
(R,S)-2-phenylpropionamide + H2O
(S)-2-phenylpropionic acid + (R)-2-phenylpropionamide + NH3
show the reaction diagram
(R,S)-2-phenylpropionamide + H2O
(S)-2-phenylpropionic acid + NH3
show the reaction diagram
-
the enzyme is highly S-stereoselective for 2-phenylpropionamide, and the racemic amide is converted to the corresponding S-acid with an enantiomeric excess of over 95% at 50% conversion of the substrate
-
-
?
(R,S)-ketoprofen amide + H2O
(S)-ketoprofen + NH3
show the reaction diagram
-
the enzyme hydrolyzes S-ketoprofen amide to its corresponding acid in an absolutely enantioselective manner
-
-
?
(R/S)-2,2-dimethylcyclopropanecarboxamide + H2O
(R)-2,2-dimethylcyclopropanecarboxylic acid + (S)-2,2-dimethylcyclopropanecarboxyamide
show the reaction diagram
-
-
-
-
-
(R/S)-piperazine-2-carboxamide + H2O
(S)-piperazine-2-carboxylic acid + (R)-piperazine-2-carboxamide
show the reaction diagram
-
-
-
-
-
(S)-(-)-2-chloropropionamide + H2O
(-)-2-chloropropionic acid + NH3
show the reaction diagram
(S)-(-)-2-hydroxy-2-(trifluoromethyl)-butanamide + H2O
(R)-(+)-2-hydroxy-2-(trifluoromethyl)-butanoic acid + NH3
show the reaction diagram
-
stereospecific reaction
enantiomerically pure product
-
?
(S)-(-)-3,3,3-trifluoro-2-amino-2-methylpropanamide + H2O
(R)-(+)-3,3,3-trifluoro-2-amino-2-methylpropanoic acid + NH3
show the reaction diagram
-
stereospecific reaction
enantiomerically pure product
-
?
(S)-(-)-3,3,3-trifluoro-2-hydroxy-2-methylpropanamide + H2O
(R)-(+)-3,3,3-trifluoro-2-hydroxy-2-methylpropanoic acid + NH3
show the reaction diagram
-
stereospecific reaction
enantiomerically pure product
-
?
(S)-2,2-dimethyl cyclopropanecarboxamide + H2O
propanecarboxylic acid
show the reaction diagram
(S)-2,2-dimethyl cyclopropanecarboxamide + H2O
propanecarboxylic acid + NH3
show the reaction diagram
(S)-2-(3-benzoylphenyl)propanamide + H2O
(S)-2-(3-benzoylphenyl)propanoic acid + NH3
show the reaction diagram
-
ketoprofen amide, R-amide is not converted
ketoprofen
-
?
(S)-2-phenylpropionamide + H2O
(S)-2-phenylpropionate + NH3
show the reaction diagram
no activity with (R)-2-phenylpropionamide
-
-
?
(S)-ibuprofen amide + H2O
(S)-ibuprofen + NH3
show the reaction diagram
-
-
-
-
?
1-naphthalene-acetamide + H2O
1-naphthaleneacetic acid + NH3
show the reaction diagram
-
about 60% of the activity with indole-3-acetamide
-
-
?
1-naphthaleneacetamide + H2O
1-naphthaleneacetic acid + NH3
show the reaction diagram
-
-
-
?
2 indole-3-acetonitrile + 3 H2O
indole-3-acetamide + indole-3-acetic acid + NH3
show the reaction diagram
-
-
-
?
2',6'-dimethylacetanilide + H2O
2,6-dimethylaniline+ acetate
show the reaction diagram
-
-
-
?
2,2-dimethylcyclopropanecarboxamide + H2O
(R)-2,2-dimethylcyclopropanecarboxylic acid + NH3
show the reaction diagram
2-aminobenzamide + H2O
2-aminobenzoic acid + NH3
show the reaction diagram
2-arachidonoylglycerol + H2O
?
show the reaction diagram
2-arachidonoylglycerol + H2O
arachidonic acid + glycerol
show the reaction diagram
2-arylpropionic amide + H2O
(S)-2-arylpropionic acid + NH3
show the reaction diagram
2-arylpropionic amide + H2O
S-2-arylpropionic acid + NH3
show the reaction diagram
2-chloroacetamide + H2O
2-chloroacetate + NH3
show the reaction diagram
-
-
-
-
?
2-chloropropionamide + H2O
2-chloropropionate + NH3
show the reaction diagram
2-hydroxybenzamide + H2O
2-hydroxybenzoic acid + NH3
show the reaction diagram
2-nitroacetanilide
2-nitrophenylamine + acetate
show the reaction diagram
2-thiophenecarboxamide + H2O
2-thiophenecarboxylate + NH3
show the reaction diagram
-
-
-
?
2-toluamide + H2O
2-toluic acid + NH3
show the reaction diagram
2-tolylamide + H2O
? + NH3
show the reaction diagram
-
-
-
-
?
3',4'-dichloropropionanilide + H2O
2,3-dichloroanilide + propanoate
show the reaction diagram
-
-
-
?
3,3,3-trifluoro-2-hydroxy-2-methylpropanamide + H2O
3,3,3-trifluoro-2-hydroxy-2-methylpropanoic acid + NH3
show the reaction diagram
-
-
-
-
?
3-hydroxymyristoyl lipid A + H2O
?
show the reaction diagram
-
3-hydroxymyristoyl group linked to the proximal glucosamine residue of de-O-acylated lipid A, hydolysis by isozyme FAAI
-
-
?
3-indolacetamide + H2O
3-indolacetate + NH3
show the reaction diagram
-
-
-
?
3-indolylacetamide + H2O
3-indolylacetic acid + NH3
show the reaction diagram
-
-
-
-
?
3-phenyl-propionamide + H2O
3-phenyl-propionate + NH3
show the reaction diagram
-
-
-
-
?
3-phenylpropionamide + H2O
3-phenylpropionate + NH3
show the reaction diagram
3-toluamide + H2O
3-toluic acid + NH3
show the reaction diagram
-
-
-
?
3-tolylamide + H2O
? + NH3
show the reaction diagram
-
-
-
-
?
4'-methylacetanilide + H2O
4-methylaniline + acetate
show the reaction diagram
-
-
-
?
4-aminobenzamide + H2O
4-aminobenzoic acid + NH3
show the reaction diagram
4-chloro-3-hydroxybutyramide + H2O
4-chloro-3-hydroxybutyrate + NH3
show the reaction diagram
-
substrate of the soluble enzyme and of the immobilized, cross-linked enzyme
-
-
?
4-chloro-3-hydroxybutyramide + H2O
4-chloro-3-hydroxybutyric acid + NH3
show the reaction diagram
-
-
-
-
4-guanidinobutyramide + H2O
?
show the reaction diagram
4-hydroxybenzamide + H2O
4-hydroxybenzoate + NH3
show the reaction diagram
-
-
-
-
?
4-hydroxybenzamide + H2O
4-hydroxybenzoic acid + NH3
show the reaction diagram
4-methylbenzamide + H2O
4-methylbenzoic acid + NH3
show the reaction diagram
4-nitroacetanilide + H2O
4-nitroaniline + acetate
show the reaction diagram
4-nitroacetanilide + H2O
acetic acid + 4-nitroaniline
show the reaction diagram
4-nitrobenzamide + H2O
4-nitrobenzoate + NH3
show the reaction diagram
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
show the reaction diagram
4-tolylamide + H2O
? + NH3
show the reaction diagram
-
-
-
-
?
5-[(4-nitrophenyl)amino]-5-oxopentanoic acid + H2O
glutarate + 4-nitroaniline
show the reaction diagram
-
-
-
?
5-[(5-carboxypentanoyl)amino]-2-nitrobenzoic acid + H2O
2-nitrobenzoate + adipate
show the reaction diagram
-
-
-
?
acetamide + H2O
?
show the reaction diagram
acetamide + H2O
acetate + NH3
show the reaction diagram
acetamide + H2O
acetic acid + NH3
show the reaction diagram
acetamide + hydroxylamine
acetylhydroxamic acid + NH3
show the reaction diagram
acetanilide + H2O
acetate + aniline
show the reaction diagram
acetate-prelabeled chicken histones + H2O
deacetylated chicken histones + acetate
show the reaction diagram
acetohydrazide + H2O
hydrazine + acetate
show the reaction diagram
-
-
-
?
acetohydroxamate + H2O
hydroxylamine + acetate
show the reaction diagram
-
-
-
?
acetylcadaverine + H2O
acetate + cadaverine
show the reaction diagram
acetylputrescine + H2O
acetate + putrescine
show the reaction diagram
acetylspermidine + H2O
acetate + spermidine
show the reaction diagram
acrylamide + H2O
acrylate + NH3
show the reaction diagram
acrylamide + H2O
acrylic acid + NH3
show the reaction diagram
acrylamide + H2O
prop-2-enoic acid + NH3
show the reaction diagram
acrylamide + hydroxylamine
acrylhydroxamic acid + NH3
show the reaction diagram
acrylamide + hydroxylamine
acryloylhydroxamic acid + NH3
show the reaction diagram
-
-
-
-
?
acylamide + H2O
carboxylic acid + NH3
show the reaction diagram
-
-
-
?
adipoamide + H2O
adipic acid + NH3
show the reaction diagram
-
-
-
-
?
adipyl-4-nitro-aniline + H2O
?
show the reaction diagram
-
-
-
?
anandamide + H2O
?
show the reaction diagram
anandamide + H2O
arachidonic acid + ethanolamine
show the reaction diagram
anthranilamide + H2O
anthranilate + NH3
show the reaction diagram
-
-
-
?
arachidonoyl-ethanolamine + H2O
arachidonic acid + ethanolamine
show the reaction diagram
benzamide
benzoic acid + NH3
show the reaction diagram
Blastobacter sp.
-
-
-
?
benzamide + H2O
benzoate + NH3
show the reaction diagram
benzamide + H2O
benzoic acid + NH3
show the reaction diagram
benzamide + hydroxylamine
benzoylhydroxamic acid + NH3
show the reaction diagram
Boc-Lys(epsilon-acetyl)-(4-methyl-coumaryl-7-amide) + H2O
Boc-Lys-(4-methyl-coumaryl-7-amide) + acetate
show the reaction diagram
butyramide + H2O
butyrate + NH3
show the reaction diagram
butyramide + H2O
butyric acid + NH3
show the reaction diagram
-
-
-
?
butyramide + H2O
n-butyrate + NH3
show the reaction diagram
-
-
-
?
butyramide + hydroxylamine
butyrylhydroxamic acid + NH3
show the reaction diagram
carboxylic acid + hydroxylamine
N-hydroxy-carboxylic acid amide + H2O
show the reaction diagram
carboxylic acid amide + H2O
carboxylic acid + NH3
show the reaction diagram
carboxylic acid amide + hydroxylamine
N-hydroxy-carboxylic acid amide + NH3
show the reaction diagram
carboxylic acid ester + hydroxylamine
N-hydroxy-carboxylic acid amide + alcohol
show the reaction diagram
cinnamamide + H2O
cinnamic acid + NH3
show the reaction diagram
cis-(1S,4R)-N-(4-(hydroxymethyl)cyclopent-2-enyl)acetamide + H2O
cis-(1S,4R)-(4-(hydroxymethyl)cyclopent-2-enyl)amine + acetate
show the reaction diagram
used as enzymatic assay
-
-
?
crotonic acid + NH3
crotonamide + H2O
show the reaction diagram
-
5% of the activity with acetic acid. Reaction with crotonamide is 0.3% of the activity with acetamide
-
-
r
cyanoacetamide + H2O
cyanoacetate + NH3
show the reaction diagram
-
low activity
-
-
?
cyclohexanamide + H2O
cyclohexanoate + NH3
show the reaction diagram
-
-
-
?
cyclohexanoamide + H2O
cyclohexanoic acid + NH3
show the reaction diagram
-
-
-
-
?
D-lactamide + H2O
D-lactate + NH3
show the reaction diagram
-
17% of the activity with acetamide, D-selectivity towards lactamide
-
-
?
D-lactamide + hydroxylamine
?
show the reaction diagram
-
-
-
-
?
D-Val-L-Leu-L-Arg 4-nitroanilide + H2O
D-Val-L-Leu-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
diacetamide + H2O
?
show the reaction diagram
-
14% of the activity with acetamide
-
-
?
DL-lactamide + H2O
DL-lactate + NH3
show the reaction diagram
DL-methioninamide + H2O
DL-methionine + NH3
show the reaction diagram
-
-
-
-
?
DL-tryptophanamide + H2O
DL-tryptophan + NH3
show the reaction diagram
-
-
-
-
?
ethyl acetate + H2O
acetic acid + ethaneamine
show the reaction diagram
ethyl acetate + H2O
ethanol + acetate
show the reaction diagram
-
-
-
?
ethyl acrylate + H2O
acrylate + ethanol
show the reaction diagram
-
-
-
?
ethyl propionate + H2O
ethanol + propionate
show the reaction diagram
-
-
-
?
fluoroacetamide + H2O
fluoroacetate + NH3
show the reaction diagram
-
165% of the activity with acetamide
-
-
?
formamide + H2O
formate + NH3
show the reaction diagram
glutaramic acid + H2O
glutaric acid + NH3
show the reaction diagram
Blastobacter sp.
-
-
-
?
glutaryl-2-naphthylamine + H2O
?
show the reaction diagram
-
-
-
?
glutaryl-aniline + H2O
?
show the reaction diagram
-
-
-
?
glutaryl-nitro-5-amino benzoic acid + H2O
?
show the reaction diagram
-
-
-
?
hexanamide + H2O
hexanoate + NH3
show the reaction diagram
hexanamide + hydroxylamine
hexanoylhydroxamic acid + NH3
show the reaction diagram
-
-
-
-
?
hexanoamide + H2O
hexanoic acid + NH3
show the reaction diagram
-
-
-
-
?
hydroxylamine + acylamide
acylhydroxamate + NH3
show the reaction diagram
-
acyltransferase activity
-
?
hydroxylamine hydrochloride + H2O
?
show the reaction diagram
ibuprofen amide + H2O
ibuprofen + NH3
show the reaction diagram
-
-
-
-
?
indole-3-acetamide + H2O
indole-3-acetate + NH3
show the reaction diagram
-
-
-
-
?
indole-3-acetamide + H2O
indole-3-acetic acid + NH3
show the reaction diagram
isobutyramide + H2O
isobutanoate + NH3
show the reaction diagram
-
16% of the activity with acetamide
-
-
?
isobutyramide + H2O
isobutyrate + NH3
show the reaction diagram
isobutyramide + H2O
isobutyric acid + NH3
show the reaction diagram
isobutyramide + hydroxylamine
isobutyrylhydroxamic acid + NH3
show the reaction diagram
isonicotinamide + H2O
isonicotinate + NH3
show the reaction diagram
isonicotinamide + H2O
isonicotinic acid + NH3
show the reaction diagram
ketoprofen amide
2-(3-benzoylphenyl)propionic acid + NH3
show the reaction diagram
L-alaninamide + H2O
L-Ala + NH3
show the reaction diagram
-
3% of the activity with acetamide
-
-
?
L-alaninamide + hydroxylamine
L-alaninoylhydroxamic acid + NH3
show the reaction diagram
-
-
-
-
?
L-alanineamide + H2O
L-alanine + NH3
show the reaction diagram
-
-
-
-
?
L-alanyl-gamma-D-glutamyl-meso-diaminopimelic acid + H2O
L-alanyl-gamma-D-glutamic acid + diaminopimelic acid
show the reaction diagram
-
murein tripeptide
-
-
?
L-asparagine + H2O
L-aspartate + NH3
show the reaction diagram
L-glutamine + H2O
L-glutamate + NH3
show the reaction diagram
L-leucinamide + H2O
L-leucine + NH3
show the reaction diagram
L-leucinamide + hydroxylamine
L-leucinoylhydroxamic acid + NH3
show the reaction diagram
-
-
-
-
?
L-leucineamide + H2O
L-leucine + NH3
show the reaction diagram
-
-
-
-
?
L-methioninamide + hydroxylamine
L-methioninoylhydroxamic acid + NH3
show the reaction diagram
-
-
-
-
?
L-prolinamide + H2O
L-proline + NH3
show the reaction diagram
L-prolinamide + hydroxylamine
L-prolinoylhydroxamic acid + NH3
show the reaction diagram
-
-
-
-
?
L-threoninamide + hydroxylamine
L-threoninoylhydroxamic acid + NH3
show the reaction diagram
-
-
-
-
?
L-valineamide + H2O
L-valine + NH3
show the reaction diagram
-
-
-
-
?
lactamide + H2O
lactate + NH3
show the reaction diagram
malonamide + H2O
malonic acid + NH3
show the reaction diagram
-
-
-
-
?
mandelamide + H2O
mandelic acid + NH3
show the reaction diagram
methacrylamide + H2O
methacrylate + NH3
show the reaction diagram
methacrylamide + H2O
methacrylic acid + NH3
show the reaction diagram
monoamidated dicarboxylate + H2O
dicarboxylate + ammonia
show the reaction diagram
Blastobacter sp.
-
-
-
?
mycothiol bimane + H2O
?
show the reaction diagram
-
-
-
-
?
N-(4-hydroxy-2-methylphenyl)arachidonylamide + H2O
4-amino-3-cresol + arachidonic acid
show the reaction diagram
N-(4-hydroxyphenyl)arachidonylamide + H2O
4-aminophenol + arachidonic acid
show the reaction diagram
-
i.e. AM404
-
-
?
n-butyramide + H2O
n-butyric acid + NH3
show the reaction diagram
-
75% of the activity with acetamide. The activity of the reverse reaction is 2% of the activity with acetic acid
-
-
r
N-lauroylethanolamine + H2O
lauric acid + ethanolamine
show the reaction diagram
-
-
-
-
?
N-methylacetamide + H2O
acetic acid + methaneamine
show the reaction diagram
N-methylacetamide + H2O
methylamine + acetate
show the reaction diagram
-
-
-
?
N-methylpropionamide + H2O
methylamine + propionate
show the reaction diagram
-
-
-
?
N-oleoyl ethanolamine + H2O
oleic acid + ethanolamine
show the reaction diagram
FAAH-2
-
-
?
N-oleoyl ethanolamine + H2O
oleic acid + NH3
show the reaction diagram
FAAH-1
-
-
?
N-oleoyl taurine + H2O
oleic acid + taurine
show the reaction diagram
FAAH-1
-
-
?
N-oleoylethanolamine + H2O
oleic acid + ethanolamine
show the reaction diagram
N-palmitolyl ethanolamine + H2O
palmitic acid + ethanolamine
show the reaction diagram
FAAH-2
-
-
?
N-palmitolyl ethanolamine + H2O
palmitic acid + NH3
show the reaction diagram
FAAH-1
-
-
?
N-palmitoylethanolamine + H2O
palmitoic acid + ethanolamine
show the reaction diagram
nicotinamide + H2O
nicotinate + NH3
show the reaction diagram
nicotinamide + H2O
nicotinic acid + NH3
show the reaction diagram
nicotinamide + hydroxylamine
nicotinoylhydroxamic acid + NH3
show the reaction diagram
-
-
-
-
?
oleamide + H2O
?
show the reaction diagram
oleamide + H2O
oleic acid + NH3
show the reaction diagram
p-nitroacetanilide + H2O
acetic acid + p-nitroaniline
show the reaction diagram
-
mutant T103I
-
-
?
p-nitroacetanilide + H2O
p-nitroaniline + acetate
show the reaction diagram
-
-
-
-
?
p-nitroacetanilide + H2O
p-nitroaniline + NH3
show the reaction diagram
-
-
-
?
p-nitrophenylacetamide + H2O
p-nitrophenylacetic acid + NH3
show the reaction diagram
palmitoylethanolamide + H2O
palmitoylethanol + NH3
show the reaction diagram
-
-
-
-
?
pentanamide + H2O
pentanoate + NH3
show the reaction diagram
phenetidine + acetate
phenacetin + H2O
show the reaction diagram
phenylacetamide
phenylacetic acid + NH3
show the reaction diagram
phenylacetamide + H2O
benzoic acid + NH3
show the reaction diagram
-
mutant W138G and T103I-W138G
-
-
?
phenylacetamide + H2O
phenylacetate + NH3
show the reaction diagram
-
-
-
?
picolinamide + H2O
picolinate + NH3
show the reaction diagram
-
-
-
?
pivalamide + H2O
pivalate + NH3
show the reaction diagram
Pro-Phe-Arg benzylamide + H2O
Pro-Phe-Arg + benzylamine
show the reaction diagram
-
-
-
-
?
prolinamide + H2O
proline + NH3
show the reaction diagram
-
-
-
-
?
propainamide + H2O
? + NH3
show the reaction diagram
-
high activity
-
-
?
propanamide + H2O
propanoic acid + NH3
show the reaction diagram
propionamide + H2O
propanoate + NH3
show the reaction diagram
-
-
-
-
?
propionamide + H2O
propionate + NH3
show the reaction diagram
propionamide + H2O
propionic acid + NH3
show the reaction diagram
propionamide + hydroxylamine
propionylhydroxamic acid + NH3
show the reaction diagram
propionohydrazide + H2O
hydrazine + propionate
show the reaction diagram
-
-
-
?
propionohydroxamate + H2O
hydroxylamine + propionate
show the reaction diagram
-
-
-
?
pyrazinamide + H2O
? + NH3
show the reaction diagram
-
-
-
-
?
pyrazinamide + H2O
pyrazinate + NH3
show the reaction diagram
pyrazinamide + H2O
pyrazinoic acid + NH3
show the reaction diagram
-
-
-
?
RCONH2 + H2O
RCOOH + NH3
show the reaction diagram
salicylamide + H2O
salicylate + NH3
show the reaction diagram
-
-
-
?
short-chain amides + hydrazine
acid hydrazides + NH3
show the reaction diagram
-
-
-
-
?
succinamic acid + H2O
succinic acid + NH3
show the reaction diagram
Blastobacter sp.
-
-
-
?
succinamide + H2O
succinic acid + NH3
show the reaction diagram
-
-
-
-
?
sulfanilamide + H2O
sulfanilate + NH3
show the reaction diagram
-
-
-
-
?
trimethylacetamide + H2O
trimethylacetic acid + NH3
show the reaction diagram
-
-
-
-
?
urea + H2O
?
show the reaction diagram
urea + H2O
carbamic acid + NH3
show the reaction diagram
-
-
-
-
?
urea + H2O
CO2 + 2 NH3
show the reaction diagram
-
reaction of EC 3.5.1.5
-
-
?
urea + H2O
formamide + NH3
show the reaction diagram
-
-
-
-
?
valeramide + H2O
valerate + NH3
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(R)-(+)-2-chloropropionamide + H2O
(+)-2-chloropropionic acid + NH3
show the reaction diagram
(S)-(-)-2-chloropropionamide + H2O
(-)-2-chloropropionic acid + NH3
show the reaction diagram
1-naphthaleneacetamide + H2O
1-naphthaleneacetic acid + NH3
show the reaction diagram
-
-
-
?
2 indole-3-acetonitrile + 3 H2O
indole-3-acetamide + indole-3-acetic acid + NH3
show the reaction diagram
-
-
-
?
2-arylpropionic amide + H2O
S-2-arylpropionic acid + NH3
show the reaction diagram
2-chloropropionamide + H2O
2-chloropropionate + NH3
show the reaction diagram
2-nitroacetanilide
2-nitrophenylamine + acetate
show the reaction diagram
3,3,3-trifluoro-2-hydroxy-2-methylpropanamide + H2O
3,3,3-trifluoro-2-hydroxy-2-methylpropanoic acid + NH3
show the reaction diagram
-
-
-
-
?
3-hydroxymyristoyl lipid A + H2O
?
show the reaction diagram
-
3-hydroxymyristoyl group linked to the proximal glucosamine residue of de-O-acylated lipid A, hydolysis by isozyme FAAI
-
-
?
4-guanidinobutyramide + H2O
?
show the reaction diagram
A0A088BHP3
probably the natural substrate
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
show the reaction diagram
acetamide + H2O
acetic acid + NH3
show the reaction diagram
-
-
-
?
acetanilide + H2O
acetate + aniline
show the reaction diagram
acetohydrazide + H2O
hydrazine + acetate
show the reaction diagram
-
-
-
?
acetohydroxamate + H2O
hydroxylamine + acetate
show the reaction diagram
-
-
-
?
acrylamide + H2O
acrylic acid + NH3
show the reaction diagram
-
-
-
?
acylamide + H2O
carboxylic acid + NH3
show the reaction diagram
-
-
-
?
anandamide + H2O
?
show the reaction diagram
anandamide + H2O
arachidonic acid + ethanolamine
show the reaction diagram
-
-
-
-
?
benzamide
benzoic acid + NH3
show the reaction diagram
Blastobacter sp.
-
-
-
?
benzamide + H2O
benzoic acid + NH3
show the reaction diagram
butyramide + H2O
n-butyrate + NH3
show the reaction diagram
-
-
-
?
carboxylic acid + hydroxylamine
N-hydroxy-carboxylic acid amide + H2O
show the reaction diagram
carboxylic acid amide + H2O
carboxylic acid + NH3
show the reaction diagram
carboxylic acid amide + hydroxylamine
N-hydroxy-carboxylic acid amide + NH3
show the reaction diagram
carboxylic acid ester + hydroxylamine
N-hydroxy-carboxylic acid amide + alcohol
show the reaction diagram
DL-lactamide + H2O
DL-lactate + NH3
show the reaction diagram
ethyl acetate + H2O
ethanol + acetate
show the reaction diagram
-
-
-
?
ethyl acrylate + H2O
acrylate + ethanol
show the reaction diagram
-
-
-
?
ethyl propionate + H2O
ethanol + propionate
show the reaction diagram
-
-
-
?
glutaramic acid + H2O
glutaric acid + NH3
show the reaction diagram
Blastobacter sp.
-
-
-
?
hydroxylamine + acylamide
acylhydroxamate + NH3
show the reaction diagram
-
acyltransferase activity
-
?
indole-3-acetamide + H2O
indole-3-acetic acid + NH3
show the reaction diagram
isobutyramide + H2O
isobutyrate + NH3
show the reaction diagram
-
-
-
?
ketoprofen amide
2-(3-benzoylphenyl)propionic acid + NH3
show the reaction diagram
monoamidated dicarboxylate + H2O
dicarboxylate + ammonia
show the reaction diagram
Blastobacter sp.
-
-
-
?
N-methylacetamide + H2O
methylamine + acetate
show the reaction diagram
-
-
-
?
N-methylpropionamide + H2O
methylamine + propionate
show the reaction diagram
-
-
-
?
phenetidine + acetate
phenacetin + H2O
show the reaction diagram
phenylacetamide
phenylacetic acid + NH3
show the reaction diagram
pivalamide + H2O
pivalate + NH3
show the reaction diagram
propionamide + H2O
propionic acid + NH3
show the reaction diagram
propionohydrazide + H2O
hydrazine + propionate
show the reaction diagram
-
-
-
?
propionohydroxamate + H2O
hydroxylamine + propionate
show the reaction diagram
-
-
-
?
RCONH2 + H2O
RCOOH + NH3
show the reaction diagram
succinamic acid + H2O
succinic acid + NH3
show the reaction diagram
Blastobacter sp.
-
-
-
?
valeramide + H2O
valerate + NH3
show the reaction diagram
additional information
?
-