Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetaldehyde | competitive inhibitor, not time dependent; competitve inhibitor, Ki: 7.0 micromol | Pseudomonas aeruginosa | |
acetaldehyde ammonia | - |
Pseudomonas aeruginosa | |
Cyanate | Ki of strain AI3 is 1.1 mM, Ki of strain AIU1N is 1.8 mM and Ki of strain OUCH4 is 50 mM; reversible inhibition, pH 7.2 and 37°C | Pseudomonas aeruginosa | |
Hydroxyurea | Ki of strain AI3 is 1.4 mM, Ki of strain AIU1N is 10.8 mM and Ki of strain OUCH4 is 337 mM, regaines full activity within 24 h at 37°C in 0.05 M Tris buffer at pH 7.2; reversible inhibition, pH 7.2 and 37°C | Pseudomonas aeruginosa | |
additional information | no inhibition with 10 mM guanidinium carbonate, 100 mM thiourea, ammonium carbamate at strain AI 3 | Pseudomonas aeruginosa | |
Urea | competitive inhibitor at pH 7.2, 90 mM acetamide protectes AI3 amidase against 2.9 mM urea over 2 h, Ki of strain AI3 is 6.6 mM, Ki of strain AIU1N is 41.3 mM and Ki of strain OUCH4 is not known, regaines full activity within 24 h at 37°C in 0.05 M Tris buffer at pH 7.2; reversible inhibition, pH 7.2 and 37°C | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
68.6 | - |
acetamide | strain AI3 | Pseudomonas aeruginosa | |
90.4 | - |
acetamide | strain AIU1N | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41600 | - |
6 * 41600, SDS-PAGE | Pseudomonas aeruginosa |
240000 | - |
SDS-PAGE | Pseudomonas aeruginosa |
240000 | - |
calculated from measurement of the subunit molecular weight, strain AI3 | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetanilide + H2O | Pseudomonas aeruginosa | - |
acetate + aniline | - |
? | |
acetanilide + H2O | Pseudomonas aeruginosa AI3 | - |
acetate + aniline | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
and the mutant strains AIU1N and OUCH4 | - |
Pseudomonas aeruginosa | - |
and strains AIU1N and OUCH4 | - |
Pseudomonas aeruginosa AI3 | - |
and the mutant strains AIU1N and OUCH4 | - |
Pseudomonas aeruginosa AI3 | - |
and strains AIU1N and OUCH4 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.8 | - |
at 3 mM formamide, formamidase activity of AI3 amidase | Pseudomonas aeruginosa |
1.5 | - |
substrate 4-nitro-acetanilide, strain OUCH4 | Pseudomonas aeruginosa |
1.5 | - |
4-nitroacetanilide, strain OUCH 4, pH 8.5 | Pseudomonas aeruginosa |
7.4 | - |
substrate 4-nitro-acetanilide, strain AIU1N | Pseudomonas aeruginosa |
7.4 | - |
4-nitroacetanilide, strain AIU 1N, pH 8.5 | Pseudomonas aeruginosa |
10.5 | - |
substrate 4-nitro-acetanilide, strain AI3 25-fold greater activity than wild-type | Pseudomonas aeruginosa |
10.5 | - |
4-nitroacetanilide, strain AI3, pH 8.5 | Pseudomonas aeruginosa |
2900 | - |
substrate acetamide, hydrolysis, strain AIU1N | Pseudomonas aeruginosa |
4100 | - |
substrate acetamide, hydrolysis, strain AI3 | Pseudomonas aeruginosa |
Storage Stability | Organism |
---|---|
-30°C, 0.05 M Tris buffer, pH 7.2, 50% v/v glycerol, 1 mM 2-mercaptoethanol | Pseudomonas aeruginosa |
purified amidase stored in 0.05 M Tris buffer, pH 7.2, containing 1 mM mercaptoethanol and 50%, v/v, glycerol, at -30°C | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitroacetanilide + H2O | - |
Pseudomonas aeruginosa | acetic acid + 4-nitroaniline | - |
? | |
4-nitroacetanilide + H2O | - |
Pseudomonas aeruginosa AI3 | acetic acid + 4-nitroaniline | - |
? | |
acetamide + H2O | - |
Pseudomonas aeruginosa | acetic acid + NH3 | - |
? | |
acetamide + H2O | - |
Pseudomonas aeruginosa AI3 | acetic acid + NH3 | - |
? | |
acetanilide + H2O | - |
Pseudomonas aeruginosa | acetate + aniline | - |
? | |
acetanilide + H2O | - |
Pseudomonas aeruginosa AI3 | acetate + aniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 41600, SDS-PAGE | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.143 | - |
acetamide | hydrolysis, strain AI3 | Pseudomonas aeruginosa | |
1.51 | - |
acetamide | hydrolysis, strain AIU1N | Pseudomonas aeruginosa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
acetaldehyde | strain AI3 | Pseudomonas aeruginosa | |
0.0051 | - |
acetaldehyde | competitive inhibitor | Pseudomonas aeruginosa | |
0.007 | - |
acetaldehyde ammonia | strain AI3 | Pseudomonas aeruginosa | |
1.1 | - |
Cyanate | strain AI3, pH 7.2 and 37°C | Pseudomonas aeruginosa | |
1.4 | - |
Hydroxyurea | strain AI3, pH 7.2 and 37°C | Pseudomonas aeruginosa | |
1.8 | - |
Cyanate | strain AIU1N, pH 7.2 and 37°C | Pseudomonas aeruginosa | |
6.6 | - |
Urea | strain AI3, pH 7.2 and 37°C | Pseudomonas aeruginosa | |
10.8 | - |
Hydroxyurea | strain AIU1N, pH 7.2 and 37°C | Pseudomonas aeruginosa | |
41.3 | - |
Urea | strain AIU1N pH 7.2 and 37°C | Pseudomonas aeruginosa | |
50 | - |
Cyanate | strain OUCH4, pH 7.2 and 37°C | Pseudomonas aeruginosa | |
337 | - |
Hydroxyurea | strain OUCH4, pH 7.2 and 37°C | Pseudomonas aeruginosa |