Literature summary for 3.5.1.4 extracted from

Holt, S.; Nilsson, J.; Omeir, R.; Tiger, G.; Fowler, C.J.
Effects of pH on the inhibition of fatty acid amidohydrolase by ibuprofen (2001), Br. J. Pharmacol., 133, 513-520.

Search for more literature for 3.5.1.4

Cloned(Commentary)

Cloned (Comment)	Organism
expression of the wild type and several mutants in Escherichia coli	Rattus norvegicus

Protein Variants

Protein Variants	Comment	Organism
C249A	inhibition of the mutant by ibuprofen is similar than that of wild type	Rattus norvegicus
S152A	inhibition of the mutant by ibuprofen is similar than that of wild type	Rattus norvegicus
S218A	inactive mutant	Rattus norvegicus

Inhibitors

Inhibitors	Comment	Organism	Structure
arachidonoyl serotonin	more potent inhibitor at pH 8.4 than at pH 5.3	Rattus norvegicus
Ibuprofen	both enantiomers, more potent inhibitors at pH 5.3 than at pH 8.4	Rattus norvegicus
oleoyltrifluoromethylketone	more potent inhibitor at pH 5.3 than at pH 8.4	Rattus norvegicus
phenylmethyl sulfonyl fluoride	more potent inhibitor at pH 8.4 than at pH 5.3	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0033	-	arachidonoyl-ethanolamine	pH 7.3, 37°C	Rattus norvegicus
0.0036	-	arachidonoyl-ethanolamine	pH 6.3, 37°C	Rattus norvegicus
0.0041	-	arachidonoyl-ethanolamine	pH 8.2, 37°C	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	adults	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Rattus norvegicus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
arachidonoyl-ethanolamine + H2O	hydrolysis of anandamide	Rattus norvegicus	arachidonic acid + ethanolamine	-	?

Synonyms

Synonyms	Comment	Organism
FAAH	-	Rattus norvegicus
fatty acid amide hydrolase	-	Rattus norvegicus

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Release 2023.1 (January 2023)