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Literature summary for 3.5.1.4 extracted from

  • Reisinger, C.; Osprian, I.; Glieder, A.; Schoemaker, H.E.; Griengl, H.; Schwab, H.
    Enzymatic hydrolysis of cyanohydrins with recombinant nitrile hydratase and amidase from Rhodococcus erythropolis (2004), Biotechnol. Lett., 26, 1675-1680.
    View publication on PubMed

Application

Application Comment Organism
biotechnology the enzyme is useful in biocatalytic nitrile hydrolysis in a system together with nitrile hydratase, EC 4.2.1.84 Rhodococcus erythropolis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21 Rhodococcus erythropolis

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis P22984 sequence identical to the sequenced enzyme fragment from strain NCIMB11540; NCIMB11540
-
Rhodococcus erythropolis NCIMB11540 P22984 sequence identical to the sequenced enzyme fragment from strain NCIMB11540; NCIMB11540
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-2-chloromandelamide + H2O
-
Rhodococcus erythropolis (R)-2-chloromandelic acid + NH3
-
?
(R)-2-chloromandelamide + H2O
-
Rhodococcus erythropolis NCIMB11540 (R)-2-chloromandelic acid + NH3
-
?
acetamide + H2O
-
Rhodococcus erythropolis ?
-
?
acetamide + H2O
-
Rhodococcus erythropolis NCIMB11540 ?
-
?
hydroxylamine hydrochloride + H2O
-
Rhodococcus erythropolis ?
-
?
hydroxylamine hydrochloride + H2O
-
Rhodococcus erythropolis NCIMB11540 ?
-
?
mandelamide + H2O
-
Rhodococcus erythropolis mandelic acid + NH3
-
?
mandelamide + H2O
-
Rhodococcus erythropolis NCIMB11540 mandelic acid + NH3
-
?
additional information the enzyme is absolutely enantioselective, biocatalytic hydrolysis of cyanohydrins of amidase with nitrile hydratase, overview Rhodococcus erythropolis ?
-
?
additional information the enzyme is absolutely enantioselective, biocatalytic hydrolysis of cyanohydrins of amidase with nitrile hydratase, overview Rhodococcus erythropolis NCIMB11540 ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Rhodococcus erythropolis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at Rhodococcus erythropolis