Application | Comment | Organism |
---|---|---|
biotechnology | the enzyme is useful in biocatalytic nitrile hydrolysis in a system together with nitrile hydratase, EC 4.2.1.84 | Rhodococcus erythropolis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21 | Rhodococcus erythropolis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | P22984 | sequence identical to the sequenced enzyme fragment from strain NCIMB11540; NCIMB11540 | - |
Rhodococcus erythropolis NCIMB11540 | P22984 | sequence identical to the sequenced enzyme fragment from strain NCIMB11540; NCIMB11540 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-chloromandelamide + H2O | - |
Rhodococcus erythropolis | (R)-2-chloromandelic acid + NH3 | - |
? | |
(R)-2-chloromandelamide + H2O | - |
Rhodococcus erythropolis NCIMB11540 | (R)-2-chloromandelic acid + NH3 | - |
? | |
acetamide + H2O | - |
Rhodococcus erythropolis | ? | - |
? | |
acetamide + H2O | - |
Rhodococcus erythropolis NCIMB11540 | ? | - |
? | |
hydroxylamine hydrochloride + H2O | - |
Rhodococcus erythropolis | ? | - |
? | |
hydroxylamine hydrochloride + H2O | - |
Rhodococcus erythropolis NCIMB11540 | ? | - |
? | |
mandelamide + H2O | - |
Rhodococcus erythropolis | mandelic acid + NH3 | - |
? | |
mandelamide + H2O | - |
Rhodococcus erythropolis NCIMB11540 | mandelic acid + NH3 | - |
? | |
additional information | the enzyme is absolutely enantioselective, biocatalytic hydrolysis of cyanohydrins of amidase with nitrile hydratase, overview | Rhodococcus erythropolis | ? | - |
? | |
additional information | the enzyme is absolutely enantioselective, biocatalytic hydrolysis of cyanohydrins of amidase with nitrile hydratase, overview | Rhodococcus erythropolis NCIMB11540 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rhodococcus erythropolis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Rhodococcus erythropolis |