Literature summary for 3.5.1.4 extracted from

Pacheco, R.; Serralheiro, M.L.M.; Karmali, A.; Haris, P.I.
Measuring enzymatic activity of a recombinant amidase using Fourier transform infrared spectroscopy (2003), Anal. Biochem., 322, 208-214.

Search for more literature for 3.5.1.4

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4	-	acetamide	no buffer added, 25°C	Pseudomonas aeruginosa
6	-	acetamide	pH 7.2, 25°C	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	-	-
Pseudomonas aeruginosa 8602	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
using affinity column chromatography	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetamide + H2O	-	Pseudomonas aeruginosa	acetate + NH3	-	?
acetamide + H2O	-	Pseudomonas aeruginosa 8602	acetate + NH3	-	?

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Release 2023.1 (January 2023)