Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
Y41C | no structural modifications in the Y41C mutant, the pH-spectrum is slightly increased | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
4-hydroxybenzamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
0.1 | - |
2-toluamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
0.1 | - |
4-hydroxybenzamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
0.2 | - |
cyclomaltohexaose | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
0.2 | - |
4-nitrobenzamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
0.2 | - |
2-toluamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
0.3 | - |
metacrylamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
0.3 | - |
cyclomaltohexaose | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
0.3 | - |
4-nitrobenzamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
0.4 | - |
nicotinamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
0.5 | - |
metacrylamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
0.6 | - |
Benzamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
0.8 | - |
indol-3-acetamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
0.8 | - |
indol-3-acetamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
0.9 | - |
Benzamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
1.5 | - |
nicotinamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
1.5 | - |
Pentanamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
2.5 | - |
Pentanamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
2.5 | - |
3-phenylpropionamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3 | - |
3-phenylpropionamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
3.2 | - |
butyramide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
4.5 | - |
hexanamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
4.5 | - |
hexanamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
6.5 | - |
Isobutyramide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
7 | - |
Isobutyramide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
10 | - |
Propionamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
10 | - |
Propionamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
14 | - |
butyramide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
22 | - |
acetamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
37 | - |
acetamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55784 | - |
x * 55784, calculated from amino acid sequence | Saccharolobus solfataricus |
83000 | - |
dimeric enzyme form, gel filtration | Saccharolobus solfataricus |
400000 | - |
octameric enzyme form, gel filtration | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | P95896 | - |
- |
Saccharolobus solfataricus P2 | P95896 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-toluamide + H2O | - |
Saccharolobus solfataricus | 2-toluic acid + NH3 | - |
? | |
3-phenylpropionamide + H2O | - |
Saccharolobus solfataricus | 3-phenylpropionate + NH3 | - |
? | |
4-hydroxybenzamide + H2O | - |
Saccharolobus solfataricus | 4-hydroxybenzoate + NH3 | - |
? | |
4-methylbenzamide + H2O | - |
Saccharolobus solfataricus | 4-methylbenzoic acid + NH3 | - |
? | |
4-nitrobenzamide + H2O | - |
Saccharolobus solfataricus | 4-nitrobenzoate + NH3 | - |
? | |
4-toluamide + H2O | - |
Saccharolobus solfataricus | 4-toluic acid + NH3 | - |
? | |
acetamide + H2O | - |
Saccharolobus solfataricus | acetic acid + NH3 | - |
? | |
acetamide + H2O | - |
Saccharolobus solfataricus P2 | acetic acid + NH3 | - |
? | |
benzamide + H2O | - |
Saccharolobus solfataricus | benzoate + NH3 | - |
? | |
butyramide + H2O | - |
Saccharolobus solfataricus | butyrate + NH3 | - |
? | |
butyramide + H2O | - |
Saccharolobus solfataricus P2 | butyrate + NH3 | - |
? | |
hexanamide + H2O | - |
Saccharolobus solfataricus | hexanoate + NH3 | - |
? | |
indole-3-acetamide + H2O | - |
Saccharolobus solfataricus | indole-3-acetate + NH3 | - |
? | |
isobutyramide + H2O | - |
Saccharolobus solfataricus | isobutyrate + NH3 | - |
? | |
isobutyramide + H2O | - |
Saccharolobus solfataricus P2 | isobutyrate + NH3 | - |
? | |
methacrylamide + H2O | - |
Saccharolobus solfataricus | methacrylate + NH3 | - |
? | |
nicotinamide + H2O | - |
Saccharolobus solfataricus | nicotinic acid + NH3 | - |
? | |
pentanamide + H2O | - |
Saccharolobus solfataricus | pentanoate + NH3 | - |
? | |
pentanamide + H2O | - |
Saccharolobus solfataricus P2 | pentanoate + NH3 | - |
? | |
propionamide + H2O | - |
Saccharolobus solfataricus | propionic acid + NH3 | - |
? | |
propionamide + H2O | - |
Saccharolobus solfataricus P2 | propionic acid + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55784, calculated from amino acid sequence | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
signature amidase | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Saccharolobus solfataricus |
95 | - |
- |
Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermal unfolding is not reversible | Saccharolobus solfataricus |
70 | - |
thermal stability of the wild-type enzyme and its mutant Y41C at pH 5.0 and 70 °C, are similar, with half-life values over 70 h | Saccharolobus solfataricus |
80 | - |
half-life of wild-type enzyme: 65 h at pH 5.0, 25 h at pH 8.0. half-life of mutant enzyme Y41C: 64 h at pH 5.0, 0.5 h at pH 8.0 | Saccharolobus solfataricus |
84 | - |
Tm-value of mutant enzyme Y41C at pH 8.0 is 83.5°C | Saccharolobus solfataricus |
90 | - |
half-life of wild-type enzyme: 60 h at pH 5.0, 0.5 h at pH 8.0. half-life of mutant enzyme Y41C: 20 h at pH 5.0, 0.25 h at pH 8.0 | Saccharolobus solfataricus |
94 | - |
Tm-value of wild-type enzyme at pH 8.0 is 94.4°C | Saccharolobus solfataricus |
97 | - |
Tm-value of mutant enzyme Y41C at pH 5.0 is 97.3°C | Saccharolobus solfataricus |
104 | - |
Tm-value of wild-type enzyme at pH 5.0 is 104.3°C | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
2-toluamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
0.8 | - |
4-nitrobenzamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
0.8 | - |
2-toluamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
1.2 | - |
indol-3-acetamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
1.9 | - |
4-hydroxybenzamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
2.1 | - |
4-hydroxybenzamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
2.2 | - |
indol-3-acetamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
2.2 | - |
nicotinamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
2.5 | - |
4-nitrobenzamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
3 | - |
metacrylamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
4.8 | - |
metacrylamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
6.4 | - |
nicotinamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
6.7 | - |
Benzamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
7.2 | - |
Benzamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
12.2 | - |
4-toluamide | wild type enzyme | Saccharolobus solfataricus | |
12.2 | - |
4-toluamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
14.2 | - |
4-toluamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
14.2 | - |
4-toluamide | mutant enzyme Y41C | Saccharolobus solfataricus | |
15.3 | - |
3-phenylpropionamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
17.5 | - |
butyramide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
18 | - |
3-phenylpropionamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
19.5 | - |
hexanamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
21.6 | - |
hexanamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
26.8 | - |
Pentanamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
28.6 | - |
Pentanamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
28.9 | - |
acetamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
30.1 | - |
acetamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
34.3 | - |
butyramide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
48.9 | - |
Isobutyramide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
58.4 | - |
Isobutyramide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
82.9 | - |
Propionamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
99.4 | - |
Propionamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
at 70°C, assay at | Saccharolobus solfataricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 8.5 | pH 3.5: wild-type enzyme shows about 45% of maximal activity, mutant enzyme Y41C shows about 20% of maximal activity. pH 8.5: wild-type enzyme shows about 40% of maximal activity, mutant enzyme Y41C shows about 50% of maximal activity | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.81 | - |
acetamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
1.31 | - |
acetamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
1.5 | - |
indol-3-acetamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
2.45 | - |
butyramide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
2.75 | - |
indol-3-acetamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
4 | - |
2-toluamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
4 | - |
4-nitrobenzamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
4 | - |
2-toluamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
4.26 | - |
nicotinamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
4.33 | - |
hexanamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
4.8 | - |
hexanamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
5.1 | - |
3-phenylpropionamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
5.47 | - |
butyramide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
5.5 | - |
nicotinamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
6 | - |
metacrylamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
6.99 | - |
Isobutyramide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
7.2 | - |
3-phenylpropionamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
7.44 | - |
Benzamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
8.29 | - |
Propionamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
8.33 | - |
4-nitrobenzamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
8.98 | - |
Isobutyramide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
9.94 | - |
Propionamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
10.7 | - |
Pentanamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
12 | - |
Benzamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
16 | - |
metacrylamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
19 | - |
4-hydroxybenzamide | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
19 | - |
Pentanamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
21 | - |
4-hydroxybenzamide | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
47.3 | - |
cyclomaltohexaose | pH 5.0, 70°C, mutant enzyme Y41C | Saccharolobus solfataricus | |
61 | - |
cyclomaltohexaose | pH 5.0, 70°C, wild-type enzyme | Saccharolobus solfataricus |