Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
T103I | site-directed mutagenesis, binding of specific monoclonal antibodies to the mutant enzyme compared to the wild-type enzyme | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas aeruginosa 8602 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli by acetamide affinity chromatography and gel filtration | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
effects of specific monoclonal antibodies on wild-type and mutant enzyme activity | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitroacetanilide + H2O | - |
Pseudomonas aeruginosa | 4-nitroaniline + acetate | - |
? | |
4-nitroacetanilide + H2O | - |
Pseudomonas aeruginosa 8602 | 4-nitroaniline + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | epitope binding of specific monoclonal antibodies to the wild-type and T103I mutant enzyme, overview | Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Pseudomonas aeruginosa |