Crystallization (Comment) | Organism |
---|---|
purified wild-type enzyme, 25 mg/ml protein in 50 mM Tris-HCl, pH 7.2, containing 5 mM DTT and 1 mM EDTA, hexagonal crystals, X-ray diffraction structure determination and analysis at 1.25-1.4 A resolution | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
6 * 38000, SDS-PAGE | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
gene amiE, inducible enzyme | - |
Purification (Comment) | Organism |
---|---|
wild-type enzyme by acetamide affinity chromatography and gel filtration | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme catalyses the hydrolysis of small aliphatic amides | Pseudomonas aeruginosa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 38000, SDS-PAGE | Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Pseudomonas aeruginosa |