Application | Comment | Organism |
---|---|---|
synthesis | the enzyme enzyme has very high potential for biotransformation of N-substituted aromatic amides and for the production of a variety of hydroxamic acids | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
1 * 55000, alpha-subunit, + 1 * 48000, beta-subunit, SDS-PAGE | Pseudomonas putida |
55000 | - |
1 * 55000, alpha-subunit, + 1 * 48000, beta-subunit, SDS-PAGE | Pseudomonas putida |
128000 | - |
native PAGE | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
isolated from rhizosphere of cyanogenic plant Circium vulgare | - |
Pseudomonas putida BR-1 | - |
isolated from rhizosphere of cyanogenic plant Circium vulgare | - |
Purification (Comment) | Organism |
---|---|
native enzyme 2.9fold by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme also shows acyl transfer activity, ATA, forming nicotinyl hydroxamic acid and ammonia from nicotinamide and hydroxylamine, kinetics and substrate specificity, detailed overview. The acyl transfer activity of the amidase from Pseudomonas putida BR-1 rapidly catalyzes the biotransformation of short chain amides, i.e. acetamide and propionamide. Unsaturated amides, i.e. acrylamide and methacrylamide act as good substrates while amides having bulky side chain, i.e. L-tryptophanamide and DL-phenylglycinamide are poor substrates | Pseudomonas putida | ? | - |
? | |
additional information | the enzyme also shows acyl transfer activity, ATA, forming nicotinyl hydroxamic acid and ammonia from nicotinamide and hydroxylamine, kinetics and substrate specificity, detailed overview. The acyl transfer activity of the amidase from Pseudomonas putida BR-1 rapidly catalyzes the biotransformation of short chain amides, i.e. acetamide and propionamide. Unsaturated amides, i.e. acrylamide and methacrylamide act as good substrates while amides having bulky side chain, i.e. L-tryptophanamide and DL-phenylglycinamide are poor substrates | Pseudomonas putida BR-1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 55000, alpha-subunit, + 1 * 48000, beta-subunit, SDS-PAGE | Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
acyl transfer activity | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
acyl transfer activity | Pseudomonas putida |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the nitrilase superfamily | Pseudomonas putida |