Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.5.1.4: amidase

This is an abbreviated version!
For detailed information about amidase, go to the full flat file.

Word Map on EC 3.5.1.4

Reaction

a monocarboxylic acid amide
+
H2O
=
a monocarboxylate
 +
NH3

Synonyms

4-guanidinobutyramide hydrolase, A-amidase, acetamidase, acid transferase, acylamidase, acylase, AMI1, amidase, amidase 1, amide hydrolase, amide transferase, amidohydrolase, AmiE, Ana, anandamide hydrolase, AtFAAH, Azl13, C-amidase, deaminase, ester transferase, FAAH, FAAH-1, FAAH-2, fatty acid amide hydrolase, fatty acid amide hydrolase 1, fatty acylamidase, half-amidase, indole-3-acetamide amidohydrolase, K1, mHG, Mhpg, More, murein peptide amidase A, mycothiol-S-conjugate amidase, N-acetylaminohydrolase, N-acylethanolamine acid amidase, N-acylethanolamine-hydrolyzing acid amidase, N-acylethanolaminehydrolyzing acid amidase, NAAA, NAE-hydrolyzing acid amidase, PamH, PYCH_10400, R-stereospecific amidase, RhAmidase, signature amidase, SsAH, SSAM, SSO2122, tissue kallikrein, Wide spectrum amidase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.4 amidase

Temperature Stability

Temperature Stability on EC 3.5.1.4 - amidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
104
Tm-value of wild-type enzyme at pH 5.0 is 104.3°C
40
purified His6-tagged enzyme, pH 8.0, stable at for 1 h
40 - 60
the purified recombinant wild-type enzyme is fairly stable up to 40°C, has 25% residual activity at 50°C after 1 h, and is completely inactivated at 60°C after 30 min
45 - 50
purified His6-tagged enzyme, pH 8.0, 1 h, 50% activity remaining
50 - 55
-
purified enzyme, stable up to 6 h at 50°C, with a t1/2 of 7 h at 55°C
50 - 60
-
quite stable at 50°C with t1/2 of 8 h, at 60°C t1/2 is 90 min
50 - 80
-
high thermal stability at 50°C and 60°C with half-lives greater than 5 h at both temperatures, the half-life values are 43 min and 10 min at 70°C and 80°C
55
-
wild-type enzyme, stable
84
Tm-value of mutant enzyme Y41C at pH 8.0 is 83.5°C
94
Tm-value of wild-type enzyme at pH 8.0 is 94.4°C
97
Tm-value of mutant enzyme Y41C at pH 5.0 is 97.3°C
additional information