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3.5.1.4: amidase

This is an abbreviated version!
For detailed information about amidase, go to the full flat file.

Word Map on EC 3.5.1.4

Reaction

a monocarboxylic acid amide
+
H2O
=
a monocarboxylate
 +
NH3

Synonyms

4-guanidinobutyramide hydrolase, A-amidase, acetamidase, acid transferase, acylamidase, acylase, AMI1, amidase, amidase 1, amide hydrolase, amide transferase, amidohydrolase, AmiE, Ana, anandamide hydrolase, AtFAAH, Azl13, C-amidase, deaminase, ester transferase, FAAH, FAAH-1, FAAH-2, fatty acid amide hydrolase, fatty acid amide hydrolase 1, fatty acylamidase, half-amidase, indole-3-acetamide amidohydrolase, K1, mHG, Mhpg, More, murein peptide amidase A, mycothiol-S-conjugate amidase, N-acetylaminohydrolase, N-acylethanolamine acid amidase, N-acylethanolamine-hydrolyzing acid amidase, N-acylethanolaminehydrolyzing acid amidase, NAAA, NAE-hydrolyzing acid amidase, PamH, PYCH_10400, R-stereospecific amidase, RhAmidase, signature amidase, SsAH, SSAM, SSO2122, tissue kallikrein, Wide spectrum amidase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.4 amidase

KM Value

KM Value on EC 3.5.1.4 - amidase

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.3
2-Aminobenzamide
pH 7.5, 70°C
1.21
2-chloroacetamide
80 - 206
2-Chloropropionamide
0.4
2-hydroxybenzamide
pH 7.5, 70°C
0.042
2-nitroacetanilide
-
-
0.9
2-phenylpropionamide
-
pH 7.2, 70°C
0.1 - 0.3
2-toluamide
0.9
3-indolacetamide
pH 7.5, 70°C
2.325
3-phenyl-propionamide
-
pH 5.0, 70°C, wild-type enzyme
2.5 - 4
3-phenylpropionamide
0.3
4-Aminobenzamide
pH 7.5, 70°C
2.3
4-Guanidinobutyramide
pH 8.0, 35°C, recombinant enzyme
0.1 - 1.5
4-hydroxybenzamide
0.2 - 0.3
4-nitrobenzamide
0.006
4-nitrophenylacetate
-
-
0.315
5-[(5-carboxypentanoyl)amino]-2-nitrobenzoic acid
pH 7.5, 37°C
0.27 - 100
acetamide
0.014
acetanilide
-
-
83
acetate
-
-
0.04
acetate-prelabeled chicken histones
-
-
39.2
acetic acid
-
-
1.2 - 93
Acrylamide
0.0033 - 0.0041
arachidonoyl-ethanolamine
0.000674 - 7.25
Benzamide
0.127
Boc-Lys(epsilon-acetyl)-(4-methyl-coumaryl-7-amide)
pH 7.5, 37°C
0.1 - 14
butyramide
0.000434 - 0.000526
cinnamamide
4
cis-(1S,4R)-N-(4-(hydroxymethyl)cyclopent-2-enyl)acetamide
pH 7.5, 37°C
5
cyclohexanamide
pH 7.5, 70°C
0.2 - 0.3
cyclomaltohexaose
8
DL-Lactamide
-
strain R312
18
ethyl acetate
-
-
218
ethyl prop-2-enoate
-
-
2.8
glutaramic acid
Blastobacter sp.
-
-
0.958
glutaryl-nitro-5-amino benzoic acid
pH 7.5, 37°C
0.3 - 11.08
hexanamide
131
hydroxylamine
-
strain R312, transfer reaction
0.8 - 1.225
indol-3-acetamide
0.08 - 1.2
Indole-3-acetamide
0.000099 - 7
Isobutyramide
0.8
Isonicotinamide
pH 7.5, 70°C
0.000096 - 0.067
ketoprofen amide
12
L-alaninamide
-
acyl transfer reaction on hydroxylamine, pH = 7
15.92
L-glutamine
pH 7.6, 60°C
1.3
L-Leucinamide
-
acyl transfer reaction on hydroxylamine, pH = 7
5.4
L-Methioninamide
-
acyl transfer reaction on hydroxylamine, pH = 7
2 - 6.7
L-prolinamide
16
L-threoninamide
-
acyl transfer reaction on hydroxylamine, pH = 7
3
lactamide
pH 7.5, 70°C
0.3 - 0.55
metacrylamide
1.2 - 9.2
methacrylamide
1078
NH3
-
-
0.3 - 135.6
nicotinamide
0.5 - 1.98
p-nitroacetanilide
1.3 - 2.5
Pentanamide
0.069
phenylacetamide
-
-
0.029
phenylacetate
-
-
2.5
picolinamide
pH 7.5, 70°C
1.1
pivalamide
-
strain R312
0.000093 - 88
Propionamide
628
propionate
-
-
16
propionic acid ethyl ester
-
-
6.2
succinamic acid
Blastobacter sp.
-
-
1.88
Urea
0.24
Valeramide
-
strain R312
additional information
additional information
-
Michaelis–Menten kinetics
-