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(R)-(+)-2-chloropropionamide + H2O
(+)-2-chloropropionic acid + NH3
(R)-2,2-dimethyl cyclopropanecarboxamide + hydroxylamine
(R)-2,2-dimethylcyclopropane hydroxamic acid + NH3
(R)-2-chloromandelamide + H2O
(R)-2-chloromandelic acid + NH3
(R)-lactamide + H2O
lactate + NH3
about 30% of the activity with (S)-lactamide
-
-
?
(R,S)-2-(3-benzoylphenyl)propanamide + H2O
(S)-2-(3-benzoylphenyl)propanoic acid + NH3
-
enantioselective for the S-enantiomer
-
-
?
(R,S)-2-phenylpropionamide + H2O
(S)-2-phenylpropionic acid + (R)-2-phenylpropionamide + NH3
(R,S)-2-phenylpropionamide + H2O
(S)-2-phenylpropionic acid + NH3
-
the enzyme is highly S-stereoselective for 2-phenylpropionamide, and the racemic amide is converted to the corresponding S-acid with an enantiomeric excess of over 95% at 50% conversion of the substrate
-
-
?
(R,S)-ketoprofen amide + H2O
(S)-ketoprofen + NH3
-
the enzyme hydrolyzes S-ketoprofen amide to its corresponding acid in an absolutely enantioselective manner
-
-
?
(R/S)-2,2-dimethylcyclopropanecarboxamide + H2O
(R)-2,2-dimethylcyclopropanecarboxylic acid + (S)-2,2-dimethylcyclopropanecarboxyamide
-
-
-
-
?
(R/S)-piperazine-2-carboxamide + H2O
(S)-piperazine-2-carboxylic acid + (R)-piperazine-2-carboxamide
-
-
-
-
?
(S)-(-)-2-chloropropionamide + H2O
(-)-2-chloropropionic acid + NH3
(S)-(-)-2-hydroxy-2-(trifluoromethyl)-butanamide + H2O
(R)-(+)-2-hydroxy-2-(trifluoromethyl)-butanoic acid + NH3
-
stereospecific reaction
enantiomerically pure product
-
?
(S)-(-)-3,3,3-trifluoro-2-amino-2-methylpropanamide + H2O
(R)-(+)-3,3,3-trifluoro-2-amino-2-methylpropanoic acid + NH3
-
stereospecific reaction
enantiomerically pure product
-
?
(S)-(-)-3,3,3-trifluoro-2-hydroxy-2-methylpropanamide + H2O
(R)-(+)-3,3,3-trifluoro-2-hydroxy-2-methylpropanoic acid + NH3
-
stereospecific reaction
enantiomerically pure product
-
?
(S)-2,2-dimethyl cyclopropanecarboxamide + hydroxylamine
(S)-2,2-dimethylcyclopropane hydroxamic acid + NH3
(S)-2-(3-benzoylphenyl)propanamide + H2O
(S)-2-(3-benzoylphenyl)propanoic acid + NH3
-
ketoprofen amide, R-amide is not converted
ketoprofen
-
?
(S)-2-phenylpropionamide + H2O
(S)-2-phenylpropionate + NH3
no activity with (R)-2-phenylpropionamide
-
-
?
(S)-ibuprofen amide + H2O
(S)-ibuprofen + NH3
-
-
-
-
?
(S)-lactamide + H2O
lactate + NH3
1-naphthalene-acetamide + H2O
1-naphthaleneacetic acid + NH3
-
about 60% of the activity with indole-3-acetamide
-
-
?
1-naphthaleneacetamide + H2O
1-naphthaleneacetic acid + NH3
-
-
-
?
2 indole-3-acetonitrile + 3 H2O
indole-3-acetamide + indole-3-acetic acid + NH3
-
-
-
?
2',6'-dimethylacetanilide + H2O
2,6-dimethylaniline + acetate
-
-
-
?
2,2-dimethylcyclopropanecarboxamide + H2O
(R)-2,2-dimethylcyclopropanecarboxylic acid + NH3
2-aminobenzamide + H2O
2-aminobenzoic acid + NH3
2-arachidonoylglycerol + H2O
?
2-arachidonoylglycerol + H2O
arachidonic acid + glycerol
2-arylpropionic amide + H2O
(S)-2-arylpropionic acid + NH3
2-chloroacetamide + H2O
2-chloroacetate + NH3
-
-
-
-
?
2-chloropropionamide + H2O
2-chloropropionate + NH3
2-hydroxybenzamide + H2O
2-hydroxybenzoic acid + NH3
2-nitroacetanilide
2-nitrophenylamine + acetate
2-thiophenecarboxamide + H2O
2-thiophenecarboxylate + NH3
-
-
-
?
2-toluamide + H2O
2-toluic acid + NH3
2-tolylamide + H2O
? + NH3
-
-
-
-
?
3',4'-dichloropropionanilide + H2O
3,4-dichloroaniline + propanoate
-
-
-
?
3,3,3-trifluoro-2-hydroxy-2-methylpropanamide + H2O
3,3,3-trifluoro-2-hydroxy-2-methylpropanoic acid + NH3
-
-
-
-
?
3-hydroxymyristoyl lipid A + H2O
?
-
3-hydroxymyristoyl group linked to the proximal glucosamine residue of de-O-acylated lipid A, hydolysis by isozyme FAAI
-
-
?
3-indolacetamide + H2O
3-indolacetate + NH3
-
-
-
?
3-indolylacetamide + H2O
3-indolylacetic acid + NH3
-
-
-
-
?
3-phenyl-propionamide + H2O
3-phenyl-propionate + NH3
-
-
-
-
?
3-phenylpropionamide + H2O
3-phenylpropionate + NH3
3-toluamide + H2O
3-toluic acid + NH3
-
-
-
?
3-tolylamide + H2O
? + NH3
-
-
-
-
?
4'-methylacetanilide + H2O
4-methylaniline + acetate
-
-
-
?
4-aminobenzamide + H2O
4-aminobenzoic acid + NH3
4-chloro-3-hydroxybutyramide + H2O
4-chloro-3-hydroxybutyrate + NH3
-
substrate of the soluble enzyme and of the immobilized, cross-linked enzyme
-
-
?
4-chloro-3-hydroxybutyramide + H2O
4-chloro-3-hydroxybutyric acid + NH3
-
-
-
?
4-guanidinobutyramide + H2O
?
4-hydroxybenzamide + H2O
4-hydroxybenzoate + NH3
-
-
-
?
4-hydroxybenzamide + H2O
4-hydroxybenzoic acid + NH3
4-methylbenzamide + H2O
4-methylbenzoic acid + NH3
4-nitroacetanilide + H2O
4-nitroaniline + acetate
4-nitroacetanilide + H2O
acetic acid + 4-nitroaniline
4-nitrobenzamide + H2O
4-nitrobenzoate + NH3
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
4-toluamide + H2O
4-toluic acid + NH3
4-tolylamide + H2O
? + NH3
-
-
-
-
?
5-[(4-nitrophenyl)amino]-5-oxopentanoic acid + H2O
glutarate + 4-nitroaniline
-
-
-
?
5-[(5-carboxypentanoyl)amino]-2-nitrobenzoic acid + H2O
2-nitrobenzoate + adipate
-
-
-
?
acetamide + H2O
acetate + NH3
acetamide + H2O
acetic acid + NH3
acetamide + hydroxylamine
acetylhydroxamic acid + NH3
acetanilide + H2O
acetate + aniline
acetate-prelabeled chicken histones + H2O
deacetylated chicken histones + acetate
acetohydrazide + H2O
hydrazine + acetate
-
-
-
?
acetohydroxamate + H2O
hydroxylamine + acetate
-
-
-
?
acetylcadaverine + H2O
acetate + cadaverine
acetylputrescine + H2O
acetate + putrescine
acetylspermidine + H2O
acetate + spermidine
acrylamide + H2O
acrylate + NH3
acrylamide + H2O
acrylic acid + NH3
acrylamide + H2O
prop-2-enoic acid + NH3
acrylamide + hydroxylamine
acrylhydroxamic acid + NH3
acrylamide + hydroxylamine
acryloylhydroxamic acid + NH3
-
-
-
-
?
acylamide + H2O
carboxylic acid + NH3
-
-
-
?
adipoamide + H2O
adipic acid + NH3
-
-
-
-
?
adipyl-4-nitro-aniline + H2O
?
-
-
-
?
anandamide + H2O
arachidonic acid + ethanolamine
anthranilamide + H2O
anthranilate + NH3
-
-
-
?
arachidonoyl-ethanolamine + H2O
arachidonic acid + ethanolamine
benzamide
benzoic acid + NH3
Blastobacter sp.
-
-
-
?
benzamide + H2O
benzoate + NH3
benzamide + H2O
benzoic acid + NH3
benzamide + hydroxylamine
benzoylhydroxamic acid + NH3
Boc-Lys(epsilon-acetyl)-(4-methyl-coumaryl-7-amide) + H2O
Boc-Lys-(4-methyl-coumaryl-7-amide) + acetate
butyramide + H2O
butyrate + NH3
butyramide + H2O
butyric acid + NH3
-
-
-
?
butyramide + H2O
n-butyrate + NH3
-
-
-
?
butyramide + hydroxylamine
butyrylhydroxamic acid + NH3
carboxylic acid + hydroxylamine
N-hydroxy-carboxylic acid amide + H2O
carboxylic acid amide + H2O
carboxylic acid + NH3
carboxylic acid amide + hydroxylamine
N-hydroxy-carboxylic acid amide + NH3
carboxylic acid ester + hydroxylamine
N-hydroxy-carboxylic acid amide + alcohol
cinnamamide + H2O
cinnamic acid + NH3
cis-(1S,4R)-N-(4-(hydroxymethyl)cyclopent-2-enyl)acetamide + H2O
cis-(1S,4R)-(4-(hydroxymethyl)cyclopent-2-enyl)amine + acetate
used as enzymatic assay
-
-
?
crotonic acid + NH3
crotonamide + H2O
-
5% of the activity with acetic acid. Reaction with crotonamide is 0.3% of the activity with acetamide
-
-
r
cyanoacetamide + H2O
cyanoacetate + NH3
-
low activity
-
-
?
cyclohexanamide + H2O
cyclohexanoate + NH3
-
-
-
?
cyclohexanoamide + H2O
cyclohexanoic acid + NH3
-
-
-
-
?
D-lactamide + H2O
D-lactate + NH3
-
17% of the activity with acetamide, D-selectivity towards lactamide
-
-
?
D-lactamide + hydroxylamine
?
-
-
-
-
?
D-Val-L-Leu-L-Arg 4-nitroanilide + H2O
D-Val-L-Leu-L-Arg + 4-nitroaniline
-
-
-
-
?
diacetamide + H2O
?
-
14% of the activity with acetamide
-
-
?
DL-lactamide + H2O
DL-lactate + NH3
DL-methioninamide + H2O
DL-methionine + NH3
-
-
-
-
?
DL-tryptophanamide + H2O
DL-tryptophan + NH3
-
-
-
-
?
ethyl acetate + H2O
acetic acid + ethanol
ethyl acetate + H2O
ethanol + acetate
-
-
-
?
ethyl acrylate + H2O
acrylate + ethanol
-
-
-
?
ethyl propionate + H2O
ethanol + propionate
-
-
-
?
fluoroacetamide + H2O
fluoroacetate + NH3
-
165% of the activity with acetamide
-
-
?
formamide + H2O
formate + NH3
glutaramic acid + H2O
glutaric acid + NH3
Blastobacter sp.
-
-
-
?
glutaryl-2-naphthylamine + H2O
?
-
-
-
?
glutaryl-aniline + H2O
?
-
-
-
?
glutaryl-nitro-5-amino benzoic acid + H2O
?
-
-
-
?
hexanamide + H2O
hexanoate + NH3
hexanamide + hydroxylamine
hexanoylhydroxamic acid + NH3
-
-
-
-
?
hexanoamide + H2O
hexanoic acid + NH3
-
-
-
-
?
hydroxylamine + acylamide
acylhydroxamate + NH3
-
acyltransferase activity
-
?
hydroxylamine hydrochloride + H2O
?
ibuprofen amide + H2O
ibuprofen + NH3
-
-
-
-
?
indole-3-acetamide + H2O
indole-3-acetate + NH3
indole-3-acetamide + H2O
indole-3-acetic acid + NH3
isobutyramide + H2O
isobutanoate + NH3
-
16% of the activity with acetamide
-
-
?
isobutyramide + H2O
isobutyrate + NH3
isobutyramide + H2O
isobutyric acid + NH3
isobutyramide + hydroxylamine
isobutyrylhydroxamic acid + NH3
isonicotinamide + H2O
isonicotinate + NH3
isonicotinamide + H2O
isonicotinic acid + NH3
ketoprofen amide
2-(3-benzoylphenyl)propionic acid + NH3
L-alaninamide + H2O
L-Ala + NH3
-
3% of the activity with acetamide
-
-
?
L-alaninamide + hydroxylamine
L-alaninoylhydroxamic acid + NH3
-
-
-
-
?
L-alanineamide + H2O
L-alanine + NH3
-
-
-
-
?
L-alanyl-gamma-D-glutamyl-meso-diaminopimelic acid + H2O
L-alanyl-D-glutamic acid + diaminopimelic acid
-
murein tripeptide
-
-
?
L-asparagine + H2O
L-aspartate + NH3
L-glutamine + H2O
L-glutamate + NH3
L-leucinamide + H2O
L-leucine + NH3
L-leucinamide + hydroxylamine
L-leucinoylhydroxamic acid + NH3
-
-
-
-
?
L-leucineamide + H2O
L-leucine + NH3
-
-
-
-
?
L-methioninamide + hydroxylamine
L-methioninoylhydroxamic acid + NH3
-
-
-
-
?
L-prolinamide + H2O
L-proline + NH3
L-prolinamide + hydroxylamine
L-prolinoylhydroxamic acid + NH3
-
-
-
-
?
L-threoninamide + hydroxylamine
L-threoninoylhydroxamic acid + NH3
-
-
-
-
?
L-valineamide + H2O
L-valine + NH3
-
-
-
-
?
lactamide + H2O
lactate + NH3
malonamide + H2O
malonic acid + NH3
-
-
-
-
?
mandelamide + H2O
mandelic acid + NH3
methacrylamide + H2O
methacrylate + NH3
methacrylamide + H2O
methacrylic acid + NH3
monoamidated dicarboxylate + H2O
dicarboxylate + ammonia
Blastobacter sp.
-
-
-
?
mycothiol bimane + H2O
?
-
-
-
-
?
N-(4-hydroxy-2-methylphenyl)arachidonylamide + H2O
4-amino-3-cresol + arachidonic acid
N-(4-hydroxyphenyl)arachidonylamide + H2O
4-aminophenol + arachidonic acid
-
i.e. AM404
-
-
?
n-butyramide + H2O
n-butyric acid + NH3
-
75% of the activity with acetamide. The activity of the reverse reaction is 2% of the activity with acetic acid
-
-
r
N-lauroylethanolamine + H2O
lauric acid + ethanolamine
-
-
-
-
?
N-methylacetamide + H2O
acetic acid + methaneamine
N-methylacetamide + H2O
methylamine + acetate
-
-
-
?
N-methylpropionamide + H2O
methylamine + propionate
-
-
-
?
N-oleoyl ethanolamine + H2O
oleic acid + ethanolamine
N-oleoyl taurine + H2O
oleic acid + taurine
FAAH-1
-
-
?
N-oleoylethanolamine + H2O
oleic acid + ethanolamine
N-palmitolyl ethanolamine + H2O
palmitic acid + ethanolamine
N-palmitoylethanolamine + H2O
palmitic acid + ethanolamine
nicotinamide + H2O
nicotinate + NH3
nicotinamide + H2O
nicotinic acid + NH3
nicotinamide + hydroxylamine
nicotinoylhydroxamic acid + NH3
-
-
-
-
?
oleamide + H2O
oleic acid + NH3
p-nitroacetanilide + H2O
acetic acid + p-nitroaniline
-
mutant T103I
-
-
?
p-nitroacetanilide + H2O
p-nitroaniline + acetate
p-nitrophenylacetamide + H2O
p-nitrophenylacetic acid + NH3
palmitoylethanolamide + H2O
palmitic acid + ethanolamine
-
-
-
-
?
pentanamide + H2O
pentanoate + NH3
phenetidine + acetate
phenacetin + H2O
phenylacetamide
phenylacetic acid + NH3
phenylacetamide + H2O
phenylacetate + NH3
picolinamide + H2O
picolinate + NH3
-
-
-
?
pivalamide + H2O
pivalate + NH3
Pro-Phe-Arg benzylamide + H2O
Pro-Phe-Arg + benzylamine
-
-
-
-
?
prolinamide + H2O
proline + NH3
-
-
-
-
?
propainamide + H2O
? + NH3
-
high activity
-
-
?
propanamide + H2O
propanoic acid + NH3
propionamide + H2O
propanoate + NH3
-
-
-
-
?
propionamide + H2O
propionate + NH3
propionamide + H2O
propionic acid + NH3
propionamide + hydroxylamine
propionylhydroxamic acid + NH3
propionohydrazide + H2O
hydrazine + propionate
-
-
-
?
propionohydroxamate + H2O
hydroxylamine + propionate
-
-
-
?
pyrazinamide + H2O
? + NH3
-
-
-
-
?
pyrazinamide + H2O
pyrazinate + NH3
pyrazinamide + H2O
pyrazinoic acid + NH3
-
-
-
?
R-lactamide + H2O
lactate + NH3
about 65% of the activity with propionamide
-
-
?
salicylamide + H2O
salicylate + NH3
-
-
-
?
short-chain amides + hydrazine
acid hydrazides + NH3
-
-
-
-
?
succinamic acid + H2O
succinic acid + NH3
Blastobacter sp.
-
-
-
?
succinamide + H2O
succinic acid + NH3
-
-
-
-
?
sulfanilamide + H2O
sulfanilate + NH3
-
-
-
-
?
trimethylacetamide + H2O
trimethylacetic acid + NH3
-
-
-
-
?
urea + H2O
carbamic acid + NH3
urea + H2O
CO2 + 2 NH3
-
reaction of EC 3.5.1.5
-
-
?
valeramide + H2O
valerate + NH3
additional information
?
-
(R)-(+)-2-chloropropionamide + H2O
(+)-2-chloropropionic acid + NH3
-
-
-
?
(R)-(+)-2-chloropropionamide + H2O
(+)-2-chloropropionic acid + NH3
-
-
-
?
(R)-2,2-dimethyl cyclopropanecarboxamide + hydroxylamine
(R)-2,2-dimethylcyclopropane hydroxamic acid + NH3
-
R-stereospecific avtivity
-
-
?
(R)-2,2-dimethyl cyclopropanecarboxamide + hydroxylamine
(R)-2,2-dimethylcyclopropane hydroxamic acid + NH3
-
R-stereospecific avtivity
-
-
?
(R)-2,2-dimethyl cyclopropanecarboxamide + hydroxylamine
(R)-2,2-dimethylcyclopropane hydroxamic acid + NH3
-
R-stereospecific avtivity
-
-
?
(R)-2,2-dimethyl cyclopropanecarboxamide + hydroxylamine
(R)-2,2-dimethylcyclopropane hydroxamic acid + NH3
-
R-stereospecific avtivity
-
-
?
(R)-2-chloromandelamide + H2O
(R)-2-chloromandelic acid + NH3
-
-
-
?
(R)-2-chloromandelamide + H2O
(R)-2-chloromandelic acid + NH3
-
-
-
?
(R,S)-2-phenylpropionamide + H2O
(S)-2-phenylpropionic acid + (R)-2-phenylpropionamide + NH3
-
enantioselective reaction with preference for the S-enantiomer
-
-
?
(R,S)-2-phenylpropionamide + H2O
(S)-2-phenylpropionic acid + (R)-2-phenylpropionamide + NH3
-
enantioselective reaction with preference for the S-enantiomer
-
-
?
(S)-(-)-2-chloropropionamide + H2O
(-)-2-chloropropionic acid + NH3
-
-
-
?
(S)-(-)-2-chloropropionamide + H2O
(-)-2-chloropropionic acid + NH3
-
-
-
?
(S)-2,2-dimethyl cyclopropanecarboxamide + hydroxylamine
(S)-2,2-dimethylcyclopropane hydroxamic acid + NH3
-
S-stereospecific activity
-
-
?
(S)-2,2-dimethyl cyclopropanecarboxamide + hydroxylamine
(S)-2,2-dimethylcyclopropane hydroxamic acid + NH3
-
S-stereospecific activity
-
-
?
(S)-2,2-dimethyl cyclopropanecarboxamide + hydroxylamine
(S)-2,2-dimethylcyclopropane hydroxamic acid + NH3
-
S-stereospecific activity
-
-
?
(S)-2,2-dimethyl cyclopropanecarboxamide + hydroxylamine
(S)-2,2-dimethylcyclopropane hydroxamic acid + NH3
-
S-stereospecific activity
-
-
?
(S)-lactamide + H2O
lactate + NH3
-
-
-
?
(S)-lactamide + H2O
lactate + NH3
about 35% of the activity with propionamide
-
-
?
2,2-dimethylcyclopropanecarboxamide + H2O
(R)-2,2-dimethylcyclopropanecarboxylic acid + NH3
-
70 min of bioconversion at 35°C, kinetic resolution of (R,S)-2,2-dimethylcyclopropanecarboxamide by the amidase affords (S)-2,2-dimethylcyclopropanecarboxamide in 41.1% yield (>99% ee) and (R)-2,2-dimethylcyclopropanecarboxylic acid in 49.9% yield (69.7% ee). The enantioselectivity is temperature dependent and is enhanced from 12.6 at 45°C to 65.9 at 14°C
-
-
?
2,2-dimethylcyclopropanecarboxamide + H2O
(R)-2,2-dimethylcyclopropanecarboxylic acid + NH3
-
70 min of bioconversion at 35°C, kinetic resolution of (R,S)-2,2-dimethylcyclopropanecarboxamide by the amidase affords (S)-2,2-dimethylcyclopropanecarboxamide in 41.1% yield (>99% ee) and (R)-2,2-dimethylcyclopropanecarboxylic acid in 49.9% yield (69.7% ee). The enantioselectivity is temperature dependent and is enhanced from 12.6 at 45°C to 65.9 at 14°C
-
-
?
2-aminobenzamide + H2O
2-aminobenzoic acid + NH3
-
-
-
-
?
2-aminobenzamide + H2O
2-aminobenzoic acid + NH3
-
-
-
?
2-arachidonoylglycerol + H2O
?
-
-
-
?
2-arachidonoylglycerol + H2O
?
-
-
-
?
2-arachidonoylglycerol + H2O
arachidonic acid + glycerol
-
-
-
?
2-arachidonoylglycerol + H2O
arachidonic acid + glycerol
-
-
-
?
2-arylpropionic amide + H2O
(S)-2-arylpropionic acid + NH3
-
enantioselective S-amidase
-
?
2-arylpropionic amide + H2O
(S)-2-arylpropionic acid + NH3
-
enantioselective S-amidase
-
?
2-chloropropionamide + H2O
2-chloropropionate + NH3
-
-
-
?
2-chloropropionamide + H2O
2-chloropropionate + NH3
-
-
-
?
2-hydroxybenzamide + H2O
2-hydroxybenzoic acid + NH3
-
-
-
-
?
2-hydroxybenzamide + H2O
2-hydroxybenzoic acid + NH3
-
-
-
?
2-nitroacetanilide
2-nitrophenylamine + acetate
-
-
-
?
2-nitroacetanilide
2-nitrophenylamine + acetate
-
-
-
?
2-toluamide + H2O
2-toluic acid + NH3
-
-
-
-
?
2-toluamide + H2O
2-toluic acid + NH3
-
-
-
?
2-toluamide + H2O
2-toluic acid + NH3
-
-
-
-
?
3-phenylpropionamide + H2O
3-phenylpropionate + NH3
-
-
-
?
3-phenylpropionamide + H2O
3-phenylpropionate + NH3
-
-
-
-
?
4-aminobenzamide + H2O
4-aminobenzoic acid + NH3
-
-
-
-
?
4-aminobenzamide + H2O
4-aminobenzoic acid + NH3
-
-
-
?
4-guanidinobutyramide + H2O
?
best substrate
-
-
?
4-guanidinobutyramide + H2O
?
probably the natural substrate
-
-
?
4-hydroxybenzamide + H2O
4-hydroxybenzoic acid + NH3
-
-
-
-
?
4-hydroxybenzamide + H2O
4-hydroxybenzoic acid + NH3
-
-
-
?
4-methylbenzamide + H2O
4-methylbenzoic acid + NH3
-
-
-
-
?
4-methylbenzamide + H2O
4-methylbenzoic acid + NH3
-
-
-
?
4-methylbenzamide + H2O
4-methylbenzoic acid + NH3
best aromatic substrate
-
-
?
4-nitroacetanilide + H2O
4-nitroaniline + acetate
-
-
-
-
?
4-nitroacetanilide + H2O
4-nitroaniline + acetate
-
-
-
-
?
4-nitroacetanilide + H2O
acetic acid + 4-nitroaniline
-
-
-
-
?
4-nitroacetanilide + H2O
acetic acid + 4-nitroaniline
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-toluamide + H2O
4-toluic acid + NH3
-
-
-
?
4-toluamide + H2O
4-toluic acid + NH3
-
-
-
-
?
acetamide + H2O
?
-
-
-
?
acetamide + H2O
?
-
-
-
?
acetamide + H2O
acetate + NH3
-
-
-
-
?
acetamide + H2O
acetate + NH3
-
high activity
-
-
?
acetamide + H2O
acetate + NH3
-
high activity
-
-
?
acetamide + H2O
acetate + NH3
-
-
-
-
?
acetamide + H2O
acetate + NH3
-
-
-
-
?
acetamide + H2O
acetate + NH3
-
-
-
-
?
acetamide + H2O
acetate + NH3
-
-
-
-
?
acetamide + H2O
acetate + NH3
-
-
-
-
?
acetamide + H2O
acetate + NH3
-
-
-
-
?
acetamide + H2O
acetate + NH3
-
best substrate
-
-
?
acetamide + H2O
acetate + NH3
-
-
-
-
?
acetamide + H2O
acetate + NH3
-
best substrate
-
-
?
acetamide + H2O
acetate + NH3
-
-
-
-
?
acetamide + H2O
acetate + NH3
about 10% of the activity with propionamide
-
-
?
acetamide + H2O
acetate + NH3
about 25% of the activity with (S)-lactamide
-
-
?
acetamide + H2O
acetate + NH3
-
-
-
?
acetamide + H2O
acetate + NH3
-
-
-
-
?
acetamide + H2O
acetate + NH3
-
-
-
-
?
acetamide + H2O
acetate + NH3
-
-
-
-
?
acetamide + H2O
acetate + NH3
-
-
-
-
?
acetamide + H2O
acetate + NH3
-
-
-
?
acetamide + H2O
acetate + NH3
-
-
-
-
?
acetamide + H2O
acetate + NH3
-
substrate of the soluble enzyme and of the immobilized, cross-linked enzyme, low activity
-
-
?
acetamide + H2O
acetate + NH3
-
-
-
-
?
acetamide + H2O
acetate + NH3
-
-
-
-
?
acetamide + H2O
acetate + NH3
-
-
-
?
acetamide + H2O
acetate + NH3
-
-
-
-
?
acetamide + H2O
acetate + NH3
-
-
-
-
?
acetamide + H2O
acetate + NH3
-
-
-
?
acetamide + H2O
acetate + NH3
-
-
-
?
acetamide + H2O
acetic acid + NH3
-
-
-
-
?
acetamide + H2O
acetic acid + NH3
-
-
-
?
acetamide + H2O
acetic acid + NH3
-
-
-
-
?
acetamide + H2O
acetic acid + NH3
-
mutant W138G and T103I
-
-
?
acetamide + H2O
acetic acid + NH3
-
-
-
-
?
acetamide + H2O
acetic acid + NH3
-
-
-
-
?
acetamide + H2O
acetic acid + NH3
-
-
-
-
?
acetamide + H2O
acetic acid + NH3
also acyl transfer activity in the presence of hydroxylamine
-
-
?
acetamide + H2O
acetic acid + NH3
also acyl transfer activity in the presence of hydroxylamine
-
-
?
acetamide + H2O
acetic acid + NH3
-
amidase-catalyzed amide formation may cause the remaining of acetamide in the acetonitrile conversion process
-
-
r
acetamide + H2O
acetic acid + NH3
-
amidase-catalyzed amide formation may cause the remaining of acetamide in the acetonitrile conversion process
-
-
r
acetamide + H2O
acetic acid + NH3
-
-
-
?
acetamide + H2O
acetic acid + NH3
-
-
-
?
acetamide + hydroxylamine
acetylhydroxamic acid + NH3
-
-
-
-
?
acetamide + hydroxylamine
acetylhydroxamic acid + NH3
-
Ping Pong Bi Bi type mechanism
-
-
?
acetamide + hydroxylamine
acetylhydroxamic acid + NH3
-
Ping Pong Bi Bi type mechanism
-
-
?
acetanilide + H2O
acetate + aniline
-
-
-
-
?
acetanilide + H2O
acetate + aniline
-
-
-
-
?
acetanilide + H2O
acetate + aniline
-
-
-
?
acetanilide + H2O
acetate + aniline
-
-
-
?
acetate-prelabeled chicken histones + H2O
deacetylated chicken histones + acetate
-
-
-
?
acetate-prelabeled chicken histones + H2O
deacetylated chicken histones + acetate
-
-
-
?
acetylcadaverine + H2O
acetate + cadaverine
-
-
-
?
acetylcadaverine + H2O
acetate + cadaverine
-
-
-
?
acetylputrescine + H2O
acetate + putrescine
-
-
-
?
acetylputrescine + H2O
acetate + putrescine
-
-
-
?
acetylspermidine + H2O
acetate + spermidine
-
-
-
?
acetylspermidine + H2O
acetate + spermidine
-
-
-
?
acrylamide + H2O
acrylate + NH3
-
-
-
-
?
acrylamide + H2O
acrylate + NH3
-
102% of the activity with acetamide
-
-
?
acrylamide + H2O
acrylate + NH3
-
-
-
-
?
acrylamide + H2O
acrylate + NH3
-
-
-
-
?
acrylamide + H2O
acrylate + NH3
-
-
-
-
?
acrylamide + H2O
acrylate + NH3
-
best substrate
-
-
?
acrylamide + H2O
acrylate + NH3
-
-
-
-
?
acrylamide + H2O
acrylate + NH3
-
-
-
-
?
acrylamide + H2O
acrylate + NH3
-
-
-
-
?
acrylamide + H2O
acrylate + NH3
-
-
-
-
?
acrylamide + H2O
acrylate + NH3
-
-
-
?
acrylamide + H2O
acrylate + NH3
-
-
-
-
?
acrylamide + H2O
acrylate + NH3
-
substrate of the soluble enzyme and of the immobilized, cross-linked enzyme
-
-
?
acrylamide + H2O
acrylate + NH3
-
-
-
-
?
acrylamide + H2O
acrylate + NH3
low activity
-
-
?
acrylamide + H2O
acrylate + NH3
-
-
-
?
acrylamide + H2O
acrylic acid + NH3
-
-
-
-
?
acrylamide + H2O
acrylic acid + NH3
-
-
-
?
acrylamide + H2O
acrylic acid + NH3
-
-
-
?
acrylamide + H2O
acrylic acid + NH3
also acyl transfer activity in the presence of hydroxylamine
-
-
?
acrylamide + H2O
acrylic acid + NH3
also acyl transfer activity in the presence of hydroxylamine
-
-
?
acrylamide + H2O
acrylic acid + NH3
-
-
-
?
acrylamide + H2O
acrylic acid + NH3
-
42% of the activity with acetamide. The activity of the reverse reaction is 3% of the activity with acetic acid
-
-
r
acrylamide + H2O
acrylic acid + NH3
-
42% of the activity with acetamide. The activity of the reverse reaction is 3% of the activity with acetic acid
-
-
r
acrylamide + H2O
prop-2-enoic acid + NH3
-
-
-
-
?
acrylamide + H2O
prop-2-enoic acid + NH3
-
mutant W138G
-
-
?
acrylamide + hydroxylamine
acrylhydroxamic acid + NH3
-
-
-
-
?
acrylamide + hydroxylamine
acrylhydroxamic acid + NH3
-
-
-
-
?
anandamide + H2O
?
-
-
-
-
?
anandamide + H2O
?
-
i.e. arachidonoylethanolamide
-
-
?
anandamide + H2O
?
-
i.e. arachidonoylethanolamide or AEA
-
-
?
anandamide + H2O
?
-
i.e. arachidonoylethanolamide
-
-
?
anandamide + H2O
?
-
i.e. arachidonoylethanolamide or AEA
-
-
?
anandamide + H2O
arachidonic acid + ethanolamine
FAAH-1
-
-
?
anandamide + H2O
arachidonic acid + ethanolamine
FAAH-2
-
-
?
anandamide + H2O
arachidonic acid + ethanolamine
substrates in order of decreasing activity: anandamide, N-oleoylethanolamine, N-palmitoylethanolamine
-
-
?
anandamide + H2O
arachidonic acid + ethanolamine
substrates in order of decreasing activity: N-palmitoylethanolamine, N-oleoylethanolamine, anandamide
-
-
?
anandamide + H2O
arachidonic acid + ethanolamine
substrates in order of decreasing activity: anandamide, N-oleoylethanolamine, N-palmitoylethanolamine
-
-
?
anandamide + H2O
arachidonic acid + ethanolamine
substrates in order of decreasing activity: N-palmitoylethanolamine, N-oleoylethanolamine, anandamide
-
-
?
anandamide + H2O
arachidonic acid + ethanolamine
-
-
-
-
?
anandamide + H2O
arachidonic acid + ethanolamine
substrates in order of decreasing activity: anandamide, N-oleoylethanolamine, N-palmitoylethanolamine
-
-
?
anandamide + H2O
arachidonic acid + ethanolamine
substrates in order of decreasing activity: N-palmitoylethanolamine, N-oleoylethanolamine, anandamide
-
-
?
anandamide + H2O
arachidonic acid + ethanolamine
substrates in order of decreasing activity: anandamide, N-oleoylethanolamine, N-palmitoylethanolamine
-
-
?
arachidonoyl-ethanolamine + H2O
arachidonic acid + ethanolamine
-
hydrolysis of anandamide
-
-
?
arachidonoyl-ethanolamine + H2O
arachidonic acid + ethanolamine
-
hydrolysis of anandamide
-
-
?
benzamide + H2O
benzoate + NH3
-
-
-
-
?
benzamide + H2O
benzoate + NH3
-
-
-
-
?
benzamide + H2O
benzoate + NH3
-
-
-
?
benzamide + H2O
benzoate + NH3
-
-
-
-
?
benzamide + H2O
benzoate + NH3
-
substrate of the soluble enzyme and of the immobilized, cross-linked enzyme
-
-
?
benzamide + H2O
benzoate + NH3
-
-
-
-
?
benzamide + H2O
benzoate + NH3
preferred substrate
-
-
?
benzamide + H2O
benzoate + NH3
-
-
-
-
?
benzamide + H2O
benzoate + NH3
-
-
-
?
benzamide + H2O
benzoate + NH3
-
-
-
-
?
benzamide + H2O
benzoate + NH3
-
-
-
?
benzamide + H2O
benzoate + NH3
-
-
-
?
benzamide + H2O
benzoate + NH3
-
-
-
?
benzamide + H2O
benzoic acid + NH3
-
-
-
?
benzamide + H2O
benzoic acid + NH3
-
-
-
-
?
benzamide + H2O
benzoic acid + NH3
-
-
-
?
benzamide + H2O
benzoic acid + NH3
-
-
-
-
?
benzamide + H2O
benzoic acid + NH3
-
-
-
-
?
benzamide + H2O
benzoic acid + NH3
also acyl transfer activity in the presence of hydroxylamine
-
-
?
benzamide + H2O
benzoic acid + NH3
also acyl transfer activity in the presence of hydroxylamine
-
-
?
benzamide + H2O
benzoic acid + NH3
-
-
-
-
?
benzamide + H2O
benzoic acid + NH3
-
-
-
-
?
benzamide + H2O
benzoic acid + NH3
-
-
-
-
?
benzamide + hydroxylamine
benzoylhydroxamic acid + NH3
-
-
-
-
?
benzamide + hydroxylamine
benzoylhydroxamic acid + NH3
-
-
-
-
?
Boc-Lys(epsilon-acetyl)-(4-methyl-coumaryl-7-amide) + H2O
Boc-Lys-(4-methyl-coumaryl-7-amide) + acetate
-
-
-
?
Boc-Lys(epsilon-acetyl)-(4-methyl-coumaryl-7-amide) + H2O
Boc-Lys-(4-methyl-coumaryl-7-amide) + acetate
-
-
-
?
butyramide + H2O
butyrate + NH3
-
-
-
-
?
butyramide + H2O
butyrate + NH3
-
high activity
-
-
?
butyramide + H2O
butyrate + NH3
-
substrate of the soluble enzyme and of the immobilized, cross-linked enzyme
-
-
?
butyramide + H2O
butyrate + NH3
-
-
-
-
?
butyramide + H2O
butyrate + NH3
-
-
-
?
butyramide + H2O
butyrate + NH3
-
-
-
-
?
butyramide + H2O
butyrate + NH3
-
-
-
?
butyramide + hydroxylamine
butyrylhydroxamic acid + NH3
-
-
-
-
?
butyramide + hydroxylamine
butyrylhydroxamic acid + NH3
-
-
-
-
?
carboxylic acid + hydroxylamine
N-hydroxy-carboxylic acid amide + H2O
-
acid transferase transferase activity
-
?
carboxylic acid + hydroxylamine
N-hydroxy-carboxylic acid amide + H2O
-
acid transferase transferase activity
-
?
carboxylic acid amide + H2O
carboxylic acid + NH3
-
-
-
?
carboxylic acid amide + H2O
carboxylic acid + NH3
-
-
-
?
carboxylic acid amide + hydroxylamine
N-hydroxy-carboxylic acid amide + NH3
-
amide transferase activity
-
?
carboxylic acid amide + hydroxylamine
N-hydroxy-carboxylic acid amide + NH3
-
amide transferase activity
-
?
carboxylic acid ester + hydroxylamine
N-hydroxy-carboxylic acid amide + alcohol
-
ester transferase activity
-
?
carboxylic acid ester + hydroxylamine
N-hydroxy-carboxylic acid amide + alcohol
-
ester transferase activity
-
?
cinnamamide + H2O
cinnamic acid + NH3
-
-
-
?
cinnamamide + H2O
cinnamic acid + NH3
-
-
-
?
DL-lactamide + H2O
DL-lactate + NH3
-
-
-
?
DL-lactamide + H2O
DL-lactate + NH3
-
-
-
?
DL-lactamide + H2O
DL-lactate + NH3
-
-
-
-
?
ethyl acetate + H2O
acetic acid + ethanol
-
-
-
-
?
ethyl acetate + H2O
acetic acid + ethanol
-
-
-
-
?
formamide + H2O
formate + NH3
-
-
-
-
?
formamide + H2O
formate + NH3
-
30% of the activity with acetamide
-
-
?
formamide + H2O
formate + NH3
-
-
-
-
?
formamide + H2O
formate + NH3
-
30% of the activity with acetamide
-
-
?
formamide + H2O
formate + NH3
-
-
-
-
?
formamide + H2O
formate + NH3
-
-
-
-
?
formamide + H2O
formate + NH3
-
-
-
-
?
formamide + H2O
formate + NH3
22.5% of the activity with acetamide
-
-
?
formamide + H2O
formate + NH3
22.5% of the activity with acetamide
-
-
?
formamide + H2O
formate + NH3
-
99% of the activity with acetamide
-
-
?
formamide + H2O
formate + NH3
-
99% of the activity with acetamide
-
-
?
hexanamide + H2O
hexanoate + NH3
-
-
-
-
?
hexanamide + H2O
hexanoate + NH3
38.7% of the activity with acetamide
-
-
?
hexanamide + H2O
hexanoate + NH3
-
-
-
?
hexanamide + H2O
hexanoate + NH3
-
-
-
-
?
hydroxylamine hydrochloride + H2O
?
-
-
-
-
?
hydroxylamine hydrochloride + H2O
?
-
-
-
?
hydroxylamine hydrochloride + H2O
?
-
-
-
?
indole-3-acetamide + H2O
indole-3-acetate + NH3
-
-
-
-
?
indole-3-acetamide + H2O
indole-3-acetate + NH3
-
-
-
-
?
indole-3-acetamide + H2O
indole-3-acetate + NH3
-
-
-
?
indole-3-acetamide + H2O
indole-3-acetic acid + NH3
-
-
-
-
?
indole-3-acetamide + H2O
indole-3-acetic acid + NH3
-
role of amidase 1 in auxin biosynthesis. Amidase 1 may be involved in de novo synthesis of indole-3-acetic acid in Arabidopsis thaliana
-
-
?
indole-3-acetamide + H2O
indole-3-acetic acid + NH3
-
-
-
?
isobutyramide + H2O
isobutyrate + NH3
-
-
-
-
?
isobutyramide + H2O
isobutyrate + NH3
-
-
-
-
?
isobutyramide + H2O
isobutyrate + NH3
-
high activity
-
-
?
isobutyramide + H2O
isobutyrate + NH3
-
high activity
-
-
?
isobutyramide + H2O
isobutyrate + NH3
-
substrate of the soluble enzyme and of the immobilized, cross-linked enzyme, preferred substrate
-
-
?
isobutyramide + H2O
isobutyrate + NH3
-
-
-
?
isobutyramide + H2O
isobutyrate + NH3
-
-
-
-
?
isobutyramide + H2O
isobutyrate + NH3
-
-
-
?
isobutyramide + H2O
isobutyrate + NH3
-
-
-
-
?
isobutyramide + H2O
isobutyrate + NH3
best aliphatic substrate
-
-
?
isobutyramide + H2O
isobutyrate + NH3
best aliphatic substrate
-
-
?
isobutyramide + H2O
isobutyrate + NH3
-
-
-
?
isobutyramide + H2O
isobutyrate + NH3
-
-
-
?
isobutyramide + H2O
isobutyric acid + NH3
-
-
-
-
?
isobutyramide + H2O
isobutyric acid + NH3
-
-
-
?
isobutyramide + hydroxylamine
isobutyrylhydroxamic acid + NH3
-
-
-
-
?
isobutyramide + hydroxylamine
isobutyrylhydroxamic acid + NH3
-
-
-
-
?
isonicotinamide + H2O
isonicotinate + NH3
-
-
-
-
?
isonicotinamide + H2O
isonicotinate + NH3
-
-
-
?
isonicotinamide + H2O
isonicotinic acid + NH3
-
-
-
-
?
isonicotinamide + H2O
isonicotinic acid + NH3
-
-
-
?
ketoprofen amide
2-(3-benzoylphenyl)propionic acid + NH3
-
-
-
?
ketoprofen amide
2-(3-benzoylphenyl)propionic acid + NH3
-
-
-
?
L-asparagine + H2O
L-aspartate + NH3
4.8% of the activity with acetamide
-
-
?
L-asparagine + H2O
L-aspartate + NH3
4.8% of the activity with acetamide
-
-
?
L-asparagine + H2O
L-aspartate + NH3
-
-
-
?
L-asparagine + H2O
L-aspartate + NH3
-
-
-
?
L-glutamine + H2O
L-glutamate + NH3
73% of the activity with acetamide
-
-
?
L-glutamine + H2O
L-glutamate + NH3
73% of the activity with acetamide
-
-
?
L-glutamine + H2O
L-glutamate + NH3
-
-
-
?
L-glutamine + H2O
L-glutamate + NH3
-
-
-
?
L-leucinamide + H2O
L-leucine + NH3
-
-
-
-
?
L-leucinamide + H2O
L-leucine + NH3
-
-
-
-
?
L-prolinamide + H2O
L-proline + NH3
-
-
-
-
?
L-prolinamide + H2O
L-proline + NH3
-
-
-
?
lactamide + H2O
lactate + NH3
-
-
-
-
?
lactamide + H2O
lactate + NH3
-
-
-
-
?
lactamide + H2O
lactate + NH3
-
-
-
?
mandelamide + H2O
mandelic acid + NH3
-
-
-
?
mandelamide + H2O
mandelic acid + NH3
-
-
-
?
methacrylamide + H2O
methacrylate + NH3
-
-
-
-
?
methacrylamide + H2O
methacrylate + NH3
-
-
-
-
?
methacrylamide + H2O
methacrylate + NH3
-
-
-
?
methacrylamide + H2O
methacrylate + NH3
-
-
-
-
?
methacrylamide + H2O
methacrylic acid + NH3
-
-
-
-
?
methacrylamide + H2O
methacrylic acid + NH3
-
17% of the activity with acetamide. The activity of the reverse reaction is 0.1% of the activity with acetic acid
-
-
?
N-(4-hydroxy-2-methylphenyl)arachidonylamide + H2O
4-amino-3-cresol + arachidonic acid
-
i.e. VDM11, 15-20% of the activity with anandamide
-
-
?
N-(4-hydroxy-2-methylphenyl)arachidonylamide + H2O
4-amino-3-cresol + arachidonic acid
-
i.e. VDM11, 15-20% of the activity with anandamide
-
-
?
N-methylacetamide + H2O
acetic acid + methaneamine
-
-
-
-
?
N-methylacetamide + H2O
acetic acid + methaneamine
-
-
-
-
?
N-oleoyl ethanolamine + H2O
oleic acid + ethanolamine
FAAH-1
-
-
?
N-oleoyl ethanolamine + H2O
oleic acid + ethanolamine
FAAH-2
-
-
?
N-oleoylethanolamine + H2O
oleic acid + ethanolamine
substrates in order of decreasing activity: anandamide, N-oleoylethanolamine, N-palmitoylethanolamine
-
-
?
N-oleoylethanolamine + H2O
oleic acid + ethanolamine
substrates in order of decreasing activity: N-palmitoylethanolamine, N-oleoylethanolamine, anandamide
-
-
?
N-oleoylethanolamine + H2O
oleic acid + ethanolamine
substrates in order of decreasing activity: anandamide, N-oleoylethanolamine, N-palmitoylethanolamine
-
-
?
N-oleoylethanolamine + H2O
oleic acid + ethanolamine
substrates in order of decreasing activity: N-palmitoylethanolamine, N-oleoylethanolamine, anandamide
-
-
?
N-oleoylethanolamine + H2O
oleic acid + ethanolamine
substrates in order of decreasing activity: anandamide, N-oleoylethanolamine, N-palmitoylethanolamine
-
-
?
N-oleoylethanolamine + H2O
oleic acid + ethanolamine
substrates in order of decreasing activity: N-palmitoylethanolamine, N-oleoylethanolamine, anandamide
-
-
?
N-oleoylethanolamine + H2O
oleic acid + ethanolamine
substrates in order of decreasing activity: anandamide, N-oleoylethanolamine, N-palmitoylethanolamine
-
-
?
N-palmitolyl ethanolamine + H2O
palmitic acid + ethanolamine
FAAH-1
-
-
?
N-palmitolyl ethanolamine + H2O
palmitic acid + ethanolamine
FAAH-2
-
-
?
N-palmitoylethanolamine + H2O
palmitic acid + ethanolamine
substrates in order of decreasing activity: anandamide, N-oleoylethanolamine, N-palmitoylethanolamine
-
-
?
N-palmitoylethanolamine + H2O
palmitic acid + ethanolamine
substrates in order of decreasing activity: N-palmitoylethanolamine, N-oleoylethanolamine, anandamide
-
-
?
N-palmitoylethanolamine + H2O
palmitic acid + ethanolamine
substrates in order of decreasing activity: anandamide, N-oleoylethanolamine, N-palmitoylethanolamine
-
-
?
N-palmitoylethanolamine + H2O
palmitic acid + ethanolamine
substrates in order of decreasing activity: N-palmitoylethanolamine, N-oleoylethanolamine, anandamide
-
-
?
N-palmitoylethanolamine + H2O
palmitic acid + ethanolamine
substrates in order of decreasing activity: anandamide, N-oleoylethanolamine, N-palmitoylethanolamine
-
-
?
N-palmitoylethanolamine + H2O
palmitic acid + ethanolamine
substrates in order of decreasing activity: N-palmitoylethanolamine, N-oleoylethanolamine, anandamide
-
-
?
N-palmitoylethanolamine + H2O
palmitic acid + ethanolamine
substrates in order of decreasing activity: anandamide, N-oleoylethanolamine, N-palmitoylethanolamine
-
-
?
nicotinamide + H2O
nicotinate + NH3
-
reaction of EC 3.5.1.19, nicotinamidase
-
-
?
nicotinamide + H2O
nicotinate + NH3
low activity, reaction of EC 3.5.1.19
-
-
?
nicotinamide + H2O
nicotinate + NH3
reaction of EC 3.5.1.19, nicotinamidase
-
-
?
nicotinamide + H2O
nicotinic acid + NH3
-
-
-
-
?
nicotinamide + H2O
nicotinic acid + NH3
-
-
-
-
?
nicotinamide + H2O
nicotinic acid + NH3
-
absolute specificity
-
-
?
nicotinamide + H2O
nicotinic acid + NH3
-
-
-
-
?
nicotinamide + H2O
nicotinic acid + NH3
-
-
-
?
nicotinamide + H2O
nicotinic acid + NH3
-
-
-
-
?
nicotinamide + H2O
nicotinic acid + NH3
Torula cremoris
-
absolute specificity
-
-
?
oleamide + H2O
?
-
-
-
-
?
oleamide + H2O
?
-
-
-
-
?
oleamide + H2O
oleic acid + NH3
-
-
-
-
?
oleamide + H2O
oleic acid + NH3
FAAH-1
-
-
?
oleamide + H2O
oleic acid + NH3
FAAH-2
-
-
?
oleamide + H2O
oleic acid + NH3
-
-
-
-
?
p-nitroacetanilide + H2O
p-nitroaniline + acetate
-
-
-
-
?
p-nitroacetanilide + H2O
p-nitroaniline + acetate
-
-
-
?
p-nitrophenylacetamide + H2O
p-nitrophenylacetic acid + NH3
-
-
-
-
?
p-nitrophenylacetamide + H2O
p-nitrophenylacetic acid + NH3
-
mutant W138G and T103I-W138G
-
-
?
pentanamide + H2O
pentanoate + NH3
-
-
-
?
pentanamide + H2O
pentanoate + NH3
-
-
-
-
?
pentanamide + H2O
pentanoate + NH3
-
-
-
?
pentanamide + H2O
pentanoate + NH3
-
-
-
?
phenetidine + acetate
phenacetin + H2O
-
transferase activity
-
?
phenetidine + acetate
phenacetin + H2O
-
transferase activity
-
?
phenylacetamide
phenylacetic acid + NH3
-
-
-
-
?
phenylacetamide
phenylacetic acid + NH3
-
-
-
-
?
phenylacetamide
phenylacetic acid + NH3
-
-
-
?
phenylacetamide
phenylacetic acid + NH3
-
-
-
?
phenylacetamide + H2O
phenylacetate + NH3
-
-
-
?
phenylacetamide + H2O
phenylacetate + NH3
-
mutant W138G and T103I-W138G
-
-
?
pivalamide + H2O
pivalate + NH3
-
-
-
?
pivalamide + H2O
pivalate + NH3
-
-
-
?
propanamide + H2O
propanoic acid + NH3
-
-
-
?
propanamide + H2O
propanoic acid + NH3
-
-
-
?
propionamide + H2O
propionate + NH3
-
best substrate
-
-
?
propionamide + H2O
propionate + NH3
-
67% of the activity with acetamide
-
-
?
propionamide + H2O
propionate + NH3
-
best substrate
-
-
?
propionamide + H2O
propionate + NH3
-
-
-
-
?
propionamide + H2O
propionate + NH3
-
-
-
-
?
propionamide + H2O
propionate + NH3
-
-
-
-
?
propionamide + H2O
propionate + NH3
-
-
-
-
?
propionamide + H2O
propionate + NH3
-
-
-
-
?
propionamide + H2O
propionate + NH3
-
best substrate
-
-
?
propionamide + H2O
propionate + NH3
-
-
-
?
propionamide + H2O
propionate + NH3
-
-
-
-
?
propionamide + H2O
propionate + NH3
-
-
-
-
?
propionamide + H2O
propionate + NH3
-
substrate of the soluble enzyme and of the immobilized, cross-linked enzyme
-
-
?
propionamide + H2O
propionate + NH3
-
-
-
-
?
propionamide + H2O
propionate + NH3
-
-
-
-
?
propionamide + H2O
propionate + NH3
-
-
-
?
propionamide + H2O
propionate + NH3
-
-
-
-
?
propionamide + H2O
propionate + NH3
-
-
-
?
propionamide + H2O
propionate + NH3
-
-
-
-
?
propionamide + H2O
propionate + NH3
-
-
-
?
propionamide + H2O
propionate + NH3
-
-
-
?
propionamide + H2O
propionic acid + NH3
-
-
-
-
?
propionamide + H2O
propionic acid + NH3
-
-
-
?
propionamide + H2O
propionic acid + NH3
about 50% of the activity with (S)-lactamide
-
-
?
propionamide + H2O
propionic acid + NH3
-
-
-
?
propionamide + H2O
propionic acid + NH3
-
-
-
?
propionamide + H2O
propionic acid + NH3
-
-
-
-
?
propionamide + H2O
propionic acid + NH3
also acyl transfer activity in the presence of hydroxylamine
-
-
?
propionamide + H2O
propionic acid + NH3
also acyl transfer activity in the presence of hydroxylamine
-
-
?
propionamide + H2O
propionic acid + NH3
-
79% of the activity with acetamide. The activity of the reverse reaction is 3% of the activity with acetic acid
-
-
r
propionamide + H2O
propionic acid + NH3
-
79% of the activity with acetamide. The activity of the reverse reaction is 3% of the activity with acetic acid
-
-
r
propionamide + H2O
propionic acid + NH3
-
-
-
?
propionamide + H2O
propionic acid + NH3
-
-
-
?
propionamide + hydroxylamine
propionylhydroxamic acid + NH3
-
-
-
-
?
propionamide + hydroxylamine
propionylhydroxamic acid + NH3
-
-
-
-
?
pyrazinamide + H2O
pyrazinate + NH3
low activity
-
-
?
pyrazinamide + H2O
pyrazinate + NH3
-
-
-
?
RCONH2 + H2O
RCOOH + NH3
-
-
-
?
RCONH2 + H2O
RCOOH + NH3
-
-
-
?
RCONH2 + H2O
RCOOH + NH3
-
-
-
?
RCONH2 + H2O
RCOOH + NH3
-
-
-
?
RCONH2 + H2O
RCOOH + NH3
-
-
-
?
RCONH2 + H2O
RCOOH + NH3
-
-
-
?
RCONH2 + H2O
RCOOH + NH3
-
-
-
?
RCONH2 + H2O
RCOOH + NH3
-
-
-
?
RCONH2 + H2O
RCOOH + NH3
-
-
-
?
urea + H2O
?
-
1.5% of the activity with acetamide
-
-
?
urea + H2O
?
-
1.5% of the activity with acetamide
-
-
?
urea + H2O
carbamic acid + NH3
-
-
-
-
?
urea + H2O
carbamic acid + NH3
-
-
-
-
?
valeramide + H2O
valerate + NH3
-
-
-
?
valeramide + H2O
valerate + NH3
-
-
-
?
valeramide + H2O
valerate + NH3
-
high activity
-
-
?
valeramide + H2O
valerate + NH3
-
high activity
-
-
?
additional information
?
-
substrate specificity towards cephalosporin analogues, overview, no activity with cephalosporin C
-
-
?
additional information
?
-
-
substrate specificity towards cephalosporin analogues, overview, no activity with cephalosporin C
-
-
?
additional information
?
-
-
acyl transfer reactions are demonstrated with acetamide, propionamide, isobutyramide, and acrylamide as substrates and hydroxylamine as the acyl acceptor. The highest reaction rate being with isobutyramide. Immobilization by entrapment in polyacrylamide gels, covalent binding on Eupergit C beads at 4°C and on Amberlite-XAD57 results in low protein binding and low activity, but immobilization on Eupergit C beads at 25°C with cross-linking resulted in high protein binding yield and high immobilized specific activity. No activity with: nicotinamide, isonicotinamide, L-asparagine, D-asparagine, DL-phenylalanine, L-prolinamide, hexanamide
-
-
?
additional information
?
-
-
not active on L-lactamide
-
-
?
additional information
?
-
-
the enzyme preferentially hydrolyzes small aliphatic amides and has a narrow substrate spectrum, overview. Poor activity with methacrylamide, N-methyl acetamide, cyanoacetamide, and butyramide. No activity with thioacetamide, trimethylacetamide, N-ethylacetamide, dimethylformamide, nicotinamide, and benzamide
-
-
?
additional information
?
-
-
the enzyme preferentially hydrolyzes small aliphatic amides and has a narrow substrate spectrum, overview. Poor activity with methacrylamide, N-methyl acetamide, cyanoacetamide, and butyramide. No activity with thioacetamide, trimethylacetamide, N-ethylacetamide, dimethylformamide, nicotinamide, and benzamide
-
-
?
additional information
?
-
-
acyl transfer reactions are demonstrated with acetamide, propionamide, isobutyramide, and acrylamide as substrates and hydroxylamine as the acyl acceptor. The highest reaction rate being with isobutyramide. Immobilization by entrapment in polyacrylamide gels, covalent binding on Eupergit C beads at 4°C and on Amberlite-XAD57 results in low protein binding and low activity, but immobilization on Eupergit C beads at 25°C with cross-linking resulted in high protein binding yield and high immobilized specific activity. No activity with: nicotinamide, isonicotinamide, L-asparagine, D-asparagine, DL-phenylalanine, L-prolinamide, hexanamide
-
-
?
additional information
?
-
-
not active on L-lactamide
-
-
?
additional information
?
-
-
the amidase of the organism exhibits dual activity i.e. hydrolase as well as acyl transfer. The acyl transfer activity of amidase forms an hydroxamate and ammonia from an amide and hydroxylamine and shows broader substrate affinity ranging from a variety of aliphatic amides (formamide, acetamide, propanamide etc.) to aromatic amides (benzamide, mandelamide, nicotinamide etc.) along with hydroxylamine for the biotransformation of these amides into corresponding hydroxamic acid, substrate specificity, overview
-
-
?
additional information
?
-
-
the amidase of the organism exhibits dual activity i.e. hydrolase as well as acyl transfer. The acyl transfer activity of amidase forms an hydroxamate and ammonia from an amide and hydroxylamine and shows broader substrate affinity ranging from a variety of aliphatic amides (formamide, acetamide, propanamide etc.) to aromatic amides (benzamide, mandelamide, nicotinamide etc.) along with hydroxylamine for the biotransformation of these amides into corresponding hydroxamic acid, substrate specificity, overview
-
-
?
additional information
?
-
-
AtFAAH is one, but not the only, modulator of endogenous N-acylethanolamine levels in plants (N-acylethanolamine depletion likely participates in the regulation of plant growth)
-
-
?
additional information
?
-
-
N-acylethanolamines and oleamide are not hydrolyzed signifiantly
-
-
?
additional information
?
-
-
no activity with methacrylamide and nicotinamide
-
-
?
additional information
?
-
-
methacrylamide and nicotinamide are no substrates
-
-
?
additional information
?
-
-
no activity with methacrylamide and nicotinamide
-
-
?
additional information
?
-
-
methacrylamide and nicotinamide are no substrates
-
-
?
additional information
?
-
-
amidation of the C-terminal carboxylic group of several N-protected di-, tri-, tetra and pentapeptides
-
-
?
additional information
?
-
-
the enzyme is specific for cleavage of the gamma-D-glutamyl-meso-diaminopimelic acid bond of free murein tripeptide but not of peptides linked to muramic acid
-
-
?
additional information
?
-
-
glycinamide, malonamide, acetamide, and acrylamide are poor substrates, while urea, mandelamide, N-methylacetamide, and thioacetamide are no substrates, substrate specificity, overview
-
-
?
additional information
?
-
-
glycinamide, malonamide, acetamide, and acrylamide are poor substrates, while urea, mandelamide, N-methylacetamide, and thioacetamide are no substrates, substrate specificity, overview
-
-
?
additional information
?
-
-
the human tissue kallikrein shows amidase activity, the enzyme hydrolyzes various synthetic substrates such as Nalpha-substituted arginine and lysine derivatives as esters, amides and fluorogenic peptides, overview
-
-
?
additional information
?
-
FAAH-1 plays a primary role in regulating endocannabinoid signaling
-
-
?
additional information
?
-
FAAH-1 plays a primary role in regulating endocannabinoid signaling
-
-
?
additional information
?
-
-
FAAH-1 plays a primary role in regulating endocannabinoid signaling
-
-
?
additional information
?
-
hardly hydrolyzes 2-arachidonoylglycerol
-
-
?
additional information
?
-
hardly hydrolyzes 2-arachidonoylglycerol
-
-
?
additional information
?
-
-
hardly hydrolyzes 2-arachidonoylglycerol
-
-
?
additional information
?
-
no FAAH-2 activity is detected with N-oleoyl taurine
-
-
?
additional information
?
-
no FAAH-2 activity is detected with N-oleoyl taurine
-
-
?
additional information
?
-
-
no FAAH-2 activity is detected with N-oleoyl taurine
-
-
?
additional information
?
-
-
the Fv dimer was constructed by using the crystallographic structure of antibody 25.3, PDB 1AFV, as a template, activity of the amide hydrolyzing antibody 312D6 with sulfonamide hapten substrates, complex formation and structure, antibody 312d6 is highly specific for p-tolylsubstituted indole sulfonamides and carboxamides, docking analysis, overview
-
-
?
additional information
?
-
-
substrate synthesis, overview, substrate specificity of the stereospecific enzyme, no activity with 2-hydroxy-2-(trifluoromethyl)-pentanamide, 3,3,3-trifluoro-2-hydroxy-2-phenyl-propionamide, 3,3,3-trichloro-2-hydroxy-2-methylpropionamide, and 3,3,3-trifluoro-2-methoxy-2-methylpropionamide, overview
-
-
?
additional information
?
-
-
FAAH is a key regulator in the development of ethanol dependence
-
-
?
additional information
?
-
hardly hydrolyzes 2-arachidonoylglycerol
-
-
?
additional information
?
-
hardly hydrolyzes 2-arachidonoylglycerol
-
-
?
additional information
?
-
-
hardly hydrolyzes 2-arachidonoylglycerol
-
-
?
additional information
?
-
the enzyme NitN has an active site and a fold characteristic of the nitrilase superfamily, but shows only amidase activity, no nitrilase activity
-
-
?
additional information
?
-
the enzyme NitN has an active site and a fold characteristic of the nitrilase superfamily, but shows only amidase activity, no nitrilase activity
-
-
?
additional information
?
-
the PamH enzyme exhibits amidase activity, aryl acylamidase activity (EC 3.5.1.13), and acyl transferase activity. It shows excellent activity toward the majority of the aromatic and aliphatic amides, such as acetamide, propionamide, phenylacetamide, and benzamide. The aromatic amides, with substitutions of one or two carbons in the ring by a nitrogen, have a negative influence on amidase activity, leading to low specific activity values for pyrazinamide and nicotinamide. No activity is detectable on long-chain aliphatic amide hexanoamides. Amino acid amides are also hydrolyzed by the enzyme. The enzyme possesses urease activity, but N-methyl substituted is not hydrolyzed by the enzyme. The amidase shows low activity on asparagines (9%), L-glutamine (17%), and D-glutamine (13%) corresponding to benzamide (100%). The anilide substrate range of the enzyme is very narrow and cannot hydrolyze butachlor, acetochlor, 4-nitroacetanilide, p-chloroacetanilide, or other structurally analogous compounds
-
-
?
additional information
?
-
-
the enzyme catalyses the hydrolysis of small aliphatic amides
-
-
?
additional information
?
-
-
the enzyme catalyses the hydrolysis of small aliphatic amides
-
-
?
additional information
?
-
-
formation of acetohydroxamic acid using acetamide and hydroxylamine as substrates
-
-
?
additional information
?
-
-
formation of acetohydroxamic acid using acetamide and hydroxylamine as substrates
-
-
?
additional information
?
-
-
stereoselective enzyme
-
-
?
additional information
?
-
-
the enzyme also shows acyl transfer activity, ATA, forming nicotinyl hydroxamic acid and ammonia from nicotinamide and hydroxylamine, kinetics and substrate specificity, detailed overview. The acyl transfer activity of the amidase from Pseudomonas putida BR-1 rapidly catalyzes the biotransformation of short chain amides, i.e. acetamide and propionamide. Unsaturated amides, i.e. acrylamide and methacrylamide act as good substrates while amides having bulky side chain, i.e. L-tryptophanamide and DL-phenylglycinamide are poor substrates
-
-
?
additional information
?
-
-
stereoselective enzyme
-
-
?
additional information
?
-
-
the enzyme also shows acyl transfer activity, ATA, forming nicotinyl hydroxamic acid and ammonia from nicotinamide and hydroxylamine, kinetics and substrate specificity, detailed overview. The acyl transfer activity of the amidase from Pseudomonas putida BR-1 rapidly catalyzes the biotransformation of short chain amides, i.e. acetamide and propionamide. Unsaturated amides, i.e. acrylamide and methacrylamide act as good substrates while amides having bulky side chain, i.e. L-tryptophanamide and DL-phenylglycinamide are poor substrates
-
-
?
additional information
?
-
-
the enzyme is enantioselective, the enzyme also shows acyl transferase and hydrolase activities, no activity with N-methylurea, fumaramide, 4-aminobenzosulfonamide, N-phenylurea, acetanilide, benzylcarbamate, and o-tolylamide, broad substrate spectrum, including aliphatic, aromatic and amino acid amides, the presence of a double bond or a methyl group near the carboxamide group of aliphatic and amino acid amides enhances the enzymatic activity, among aromatic amides with substitutions at the 2-, 3-, or 4-position, the 4-substituted amides are the preferred substrates, detailed overview
-
-
?
additional information
?
-
no activity with: urea, benzamide, pyrazinamide, nicotinamide, cinnamamide, salicylamide
-
-
?
additional information
?
-
no activity with: urea, benzamide, pyrazinamide, nicotinamide, cinnamamide, salicylamide
-
-
?
additional information
?
-
-
no activity with: urea, benzamide, pyrazinamide, nicotinamide, cinnamamide, salicylamide
-
-
?
additional information
?
-
hardly hydrolyzes 2-arachidonoylglycerol
-
-
?
additional information
?
-
hardly hydrolyzes 2-arachidonoylglycerol
-
-
?
additional information
?
-
the enzyme is absolutely enantioselective, biocatalytic hydrolysis of cyanohydrins of amidase with nitrile hydratase, overview
-
-
?
additional information
?
-
-
the enzyme is absolutely enantioselective, biocatalytic hydrolysis of cyanohydrins of amidase with nitrile hydratase, overview
-
-
?
additional information
?
-
enantioselectivity toward (R)-4-chloro-3-hydroxybutyramide: e.e value 52% (conversion yield 57%)
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enantioselectivity toward (R)-4-chloro-3-hydroxybutyramide: e.e value 52% (conversion yield 57%)
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the enzyme is absolutely enantioselective, biocatalytic hydrolysis of cyanohydrins of amidase with nitrile hydratase, overview
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enantioselectivity toward (R)-4-chloro-3-hydroxybutyramide: e.e value 52% (conversion yield 57%)
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no activity with nicotinamide or benzamide
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the Ser-cis Ser-Lys catalytic triad is located in the large domain. But the substrate-binding pocket is relatively narrow, due to the presence of the helix alpha13 in the small domain. The hydrophobic residues from the small domain are involved in recognizing the substrate. The small domain likely participates in substrate recognition and is related to the difference of substrate specificities among the AS family amidases
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no activity with nicotinamide or benzamide
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the amidase shows high enantioselectivity for (S)-arylpropionic amides, it is active on nitriles, molecular modelling, overview
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the enzyme transfers the acyl group also to hydroxylamine and hydrazine
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the enzyme transfers the acyl group also to hydroxylamine and hydrazine
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the enzyme transfers the acyl group also to hydroxylamine and hydrazine
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involved in the hydrolysis of the cell wall
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substrate specificity, overview. The recombinant enzyme displays an unusually wide substrate spectrum. It is moderately active on short-chain aliphatic amides and weakly active hydrolyzing aromatic and heterocyclic amides. Recombinant His6-tagged enzyme Azl13 also catalyzes acyl transfer to hydroxylamine from acetamide or the herbicide propanil. The enzyme also shows aryl acylamide hydrolase activity, EC 3.5.1.13, and hydroxylamine acyl transferase activity, EC 2.3.1.56. No activity with L-glutamine and L-asparagine
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other fatty acid ethanolamides may act as substrate
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