Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.5.1.4: amidase

This is an abbreviated version!
For detailed information about amidase, go to the full flat file.

Word Map on EC 3.5.1.4

Reaction

a monocarboxylic acid amide
+
H2O
=
a monocarboxylate
 +
NH3

Synonyms

4-guanidinobutyramide hydrolase, A-amidase, acetamidase, acid transferase, acylamidase, acylase, AMI1, amidase, amidase 1, amide hydrolase, amide transferase, amidohydrolase, AmiE, Ana, anandamide hydrolase, AtFAAH, Azl13, C-amidase, deaminase, ester transferase, FAAH, FAAH-1, FAAH-2, fatty acid amide hydrolase, fatty acid amide hydrolase 1, fatty acylamidase, half-amidase, indole-3-acetamide amidohydrolase, K1, mHG, Mhpg, More, murein peptide amidase A, mycothiol-S-conjugate amidase, N-acetylaminohydrolase, N-acylethanolamine acid amidase, N-acylethanolamine-hydrolyzing acid amidase, N-acylethanolaminehydrolyzing acid amidase, NAAA, NAE-hydrolyzing acid amidase, PamH, PYCH_10400, R-stereospecific amidase, RhAmidase, signature amidase, SsAH, SSAM, SSO2122, tissue kallikrein, Wide spectrum amidase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.4 amidase

Temperature Range

Temperature Range on EC 3.5.1.4 - amidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15 - 60
activity range
15 - 70
activity range, profile overview
30 - 70
-
activity range
30 - 80
-
10% of maximal activity at 30°C and 80°C
35 - 60
37 - 50
drastic drop in activity above 50°C, maximal activity at 44°C, assay at 37°C
37 - 80
-
25% of maximal activity at 37°C, maximal activity at 75°C, about 90% of maximal activity at 80°C
40 - 80
-
40°C: about 70% of maximal activity, soluble enzyme. 40°C: about 30% of maximal activity, enzyme immobilized on Eupergit C beads. 80°C: about 50% of maximal activity, soluble enzyme. 80°C: about 55% of maximal activity, enzyme immobilized on Eupergit C beads
40 - 90
-
activity range
45 - 65
-
45°C: about 45% of maximal activity, 65°C: about 70% of maximal activity
50 - 95
50°C: 53% of maximal activity, 60°C: about 80% of maximal activity, 70°C: about 90% of maximal activity, 95°C: about 89% of maximal activity
75 - 95
75°C: about 50% of maximal activity, 95°C: maximal activity, 100°C: less than 10% of activity
additional information