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3.5.1.4: amidase

This is an abbreviated version!
For detailed information about amidase, go to the full flat file.

Word Map on EC 3.5.1.4

Reaction

a monocarboxylic acid amide
+
H2O
=
a monocarboxylate
 +
NH3

Synonyms

4-guanidinobutyramide hydrolase, A-amidase, acetamidase, acid transferase, acylamidase, acylase, AMI1, amidase, amidase 1, amide hydrolase, amide transferase, amidohydrolase, AmiE, Ana, anandamide hydrolase, AtFAAH, Azl13, C-amidase, deaminase, ester transferase, FAAH, FAAH-1, FAAH-2, fatty acid amide hydrolase, fatty acid amide hydrolase 1, fatty acylamidase, half-amidase, indole-3-acetamide amidohydrolase, K1, mHG, Mhpg, More, murein peptide amidase A, mycothiol-S-conjugate amidase, N-acetylaminohydrolase, N-acylethanolamine acid amidase, N-acylethanolamine-hydrolyzing acid amidase, N-acylethanolaminehydrolyzing acid amidase, NAAA, NAE-hydrolyzing acid amidase, PamH, PYCH_10400, R-stereospecific amidase, RhAmidase, signature amidase, SsAH, SSAM, SSO2122, tissue kallikrein, Wide spectrum amidase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.4 amidase

Specific Activity

Specific Activity on EC 3.5.1.4 - amidase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000028
-
substrate indoleacetonitrile at 30°C, activity after purification
0.00003
-
growth substrate 2-monochloropropionic acid, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PP3
0.000031
-
substrate indole-3-acetamide at 30°C, activity after purification
0.00024
-
growth substrate succinate, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PP3
0.00027
-
growth substrate 2-chloropropionamide, 2-chloropropionamide-amidase activity, measured with 14 mM 2-chloropropionamide, strain PP3
0.00074
-
membrane fraction
0.0017
-
growth substrate propionamide, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PAU3
0.0021
-
D191E mutant, supernatant of cell-free extract, 0.75% of wild-type activity
0.00243
-
harboring pALJ20, substrate cyclohexanecarboxamide with cobalt
0.004
acetylspermidine, pH 7.5
0.008
acetylcadaverine, pH 7.5
0.011
acetylputrescine, pH 7.5
0.0135
-
substrate cyclohexanecarboxamide with cobalt
0.019
-
growth substrate succinate, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PPW3
0.023
-
growth substrate 2-monochloropropionic acid, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PPW3
0.0306
-
C203A mutant, supernatant of cell-free extract, 10.8% of wild-type activity
0.031
-
growth substrate succinate, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PAU5
0.039
-
growth substrate propionamide, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PAU5
0.05
-
substrate acetamide, crude extract
0.078
-
A-amidase, substrate was 2-chloropropionamide
0.12
-
growth substrate 2-chloropropionamide, 2-chloropropionamide-amidase activity, measured with 14 mM 2-chloropropionamide, strain PPW3
0.14
-
growth substrate 2-monochloropropionic acid, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PAU5
0.162
-
D191E mutant, purified, 1.33% of purified wild-type amidase
0.18
-
substrate urea
0.19
-
>99% enantiomeric excess
0.23
-
strain N6, isobutyramide, pH 7.4, room temperature, 30 min
0.283
-
wild-type amidase
0.3
-
substrate valeramide, strain R312
0.35
-
strain N6-ureB::TnKm-4, isobutyramide, pH 7.4, room temperature, 30 min
0.383
-
strain JM 109 harboring pKPO2(6)
0.468
-
harboring pALJ30, substrate cyclohexanecarboxamide with cobalt
0.55
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate 3',4'-dichloropropionanilide
0.6
-
substrate pivalamide, strain R312
0.67
-
activity in cell extract, substrate 1 mM phenylacetamide
0.69
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate 2',6'-dimethylacetanilide
0.81
-
activity in cell extract, substrate 1 mM ketoprofen amide
0.87
recombinant purified His-tagged enzyme, pH 8.0, 35°C, substrate 4'-methylacetanilide
0.89
-
strain B6, substrate para-nitroacetanilide, pH 7.2, 25°C
0.91
-
strain L10, substrate para-nitroacetanilide, pH 7.2, 25°C
0.914
-
strain PPW, activity of C-amidase after preparative PAGE, final yield 17% of original activity
1.18
85°C, pH not specified in the publication, substrate: benzamide
1.3
-
activity in cell extract, substrate 1 mM naproxen amide
1.4
-
C203Amutant, purified, 11.5% of purified wild-type amidase
1.51
-
mutant strain MM15 measured with acrylamide as substrate, growth conditions is continuous
1.89
-
strain PPW3, C-amidase, substrate 2-chloropropionamide
10
-
purified enzyme, pH 7.5, 30°C
1000
substrate: acetamide, pH 7.6, 60°C
10000
-
strain RB971 harboring pGL100 without lactose
11
-
strain N6, acrylamide, pH 7.4, room temperature, 30 min
11.6
-
wild-type amidase after purification
12
-
strain N6, propionamide, pH 7.4, room temperature, 30 min
121
-
substrate 2-nitroacetanilide, pure enzyme, pH 8.6 and 30°C
125
-
substrate acetanilide, after disc electrophoresis of purified enzyme
131
-
substrate acetanilide, after purification
133
-
substrate acetanilide, pure enzyme, pH 8.6 and 30°C
135
-
substrate acetamide, strain R312, transfer reaction
14.33
purified recombinant wild-type enzyme, pH 7.0, 35°C, substrate acrylamides
14.8
-
purified recombinant His6-tagged enzyme
143
hexanamide: pH 7.6, 60°C
1430
L-glutamine: pH 7.6, 60°C
149
-
substrate phenylacetate, pure enzyme, pH 8.6 and 30°C
16.39
-
substrate hexanamide
16.5
-
specific activity of S(+)-2-phenylpropionic acid
16.7
-
substrate propionamide, strain R312
1600
-
strain HB101 harboring pGL80
17
-
strain N6-ureB::TnKm-4, acetamide, pH 7.4, room temperature, 30 min
17.7
-
turnover propionamide to propionate and ammonia
1700
-
strain HB101 harboring pGL81
181
-
substrate 4-nitrophenylacetate, pure enzyme, pH 8.6 and 30°C
19.4
-
purified enzyme, substrate benzamide
2.1
-
mutant strain MM15 measured with acetamide as substrate
2.35
-
strain OUCH, substrate para-nitroacetanilide, pH 7.2, 25°C
23.5
-
purified enzyme, substrate methacrylamide
25.4
-
purified enzyme, substrate propionamide
26.8
-
purified enzyme, substrate acrylamide
274.1
-
micromol of NH3 * min-1 * mg of protein-1
2900
-
substrate acetamide, hydrolysis, strain AIU1N
3.5
purified recombinant enzyme, substrate glutaryl-nitro-5-amino benzoic acid
3.59
-
mutant strain MM15 measured with acrylamide as substrate, growth conditions is fed-batch
30.49
-
purified native enzyme, pH 8.0, 50°C
3500
-
micromol of NH3 * min-1 * mg of protein-1
37.5
-
reombinant enzyme in crude extract, at pH 7 and 50°C
37.6
-
micromol of NH3 * min-1 * mg of protein-1
39.82
purified recombinant wild-type enzyme, pH 7.0, 35°C, substrate benzamide
4.1
-
activity after purification, substrate 5 mM phenylacetamide
4.67
purified recombinant enzyme, substrate adipoyl-nitro-5-amino benzoic acid
4.83
-
substrate methacrylamide
4.89
-
strain AIU1N, substrate para-nitroacetanilide, pH 7.2, 25°C
4100
-
substrate acetamide, hydrolysis, strain AI3
48.66
-
purified native enzyme, pH 6.5, 70°C, substrate nicotinamide
50
-
strain N6, urea, pH 7.4, room temperature, 30 min
5000
-
substrate isobutyramide
5900
-
substrate acrylamide
6.5
-
strain N6, acetamide, pH 7.4, room temperature, 30 min
6.69
-
substrate cyclohexanamide
600
-
strain RB971 harboring pGL100 with lactose
65
-
strain N6-836, urea, pH 7.4, room temperature, 30 min
67
acrylamide: pH 7.6, 60°C
7.7
-
substrate 4-nitroacetanilide, crude extract
73
-
substrate phenacetin, pure enzyme, pH 8.6 and 30°C
7700
-
substrate butyramide
8.04
-
strain AI3, substrate para-nitroacetanilide, pH 7.2, 25°C
additional information