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3.5.1.4: amidase

This is an abbreviated version!
For detailed information about amidase, go to the full flat file.

Word Map on EC 3.5.1.4

Reaction

a monocarboxylic acid amide
+
H2O
=
a monocarboxylate
 +
NH3

Synonyms

4-guanidinobutyramide hydrolase, A-amidase, acetamidase, acid transferase, acylamidase, acylase, AMI1, amidase, amidase 1, amide hydrolase, amide transferase, amidohydrolase, AmiE, Ana, anandamide hydrolase, AtFAAH, Azl13, C-amidase, deaminase, ester transferase, FAAH, FAAH-1, FAAH-2, fatty acid amide hydrolase, fatty acid amide hydrolase 1, fatty acylamidase, half-amidase, indole-3-acetamide amidohydrolase, K1, mHG, Mhpg, More, murein peptide amidase A, mycothiol-S-conjugate amidase, N-acetylaminohydrolase, N-acylethanolamine acid amidase, N-acylethanolamine-hydrolyzing acid amidase, N-acylethanolaminehydrolyzing acid amidase, NAAA, NAE-hydrolyzing acid amidase, PamH, PYCH_10400, R-stereospecific amidase, RhAmidase, signature amidase, SsAH, SSAM, SSO2122, tissue kallikrein, Wide spectrum amidase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.4 amidase

pH Range

pH Range on EC 3.5.1.4 - amidase

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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 8
pH 3.0-5.0: about 50% of maximal activity, pH 8.0: about 65% of maximal activity
3 - 9
-
15% of maximal activity at pH 3.0, 65% at pH 9.0
3.4 - 11
-
100% activity at pH values between 5.9 and 9.9
3.5 - 8.5
pH 3.5: wild-type enzyme shows about 45% of maximal activity, mutant enzyme Y41C shows about 20% of maximal activity. pH 8.5: wild-type enzyme shows about 40% of maximal activity, mutant enzyme Y41C shows about 50% of maximal activity
4 - 10.5
-
activity range
4 - 9
-
pH 4.0: about 60% of maximal activity, soluble enzyme. pH 4.0: about 75% of maximal activity, enzyme immobilized on Eupergit C beads. and enzyme immobilized on Eupergit C beads. pH 9.0: about 40% of maximal activity, soluble enzyme. pH 9.0: about 90% of maximal activity, enzyme immobilized on Eupergit C beads
4 - 9.5
pH 4: about 50% of maximal activity, pH 9.5: about 50% of maximal activity
4.5 - 11.5
-
activity range
5 - 8.8
activity range, substrate acrylamide
5 - 9
-
high activity within this range
5.5 - 10
5.5 - 9
-
-
5.7 - 10.5
-
activity range, 61% of maximal activity at pH 5.7, 30% at pH 10.5
6 - 8
drastic drop in activity below pH 6.0, maximal activity at pH 8.0
6 - 9
-
in absence of glycylglycine
6.7 - 10
-
-
7 - 7.5
-
-
7 - 8.6
-
against the substrate propionamide
7.3 - 8
-
in presence of glycylglycine
7.5 - 8
-
activity range, at pH 5.7 and pH 9.5, the enzyme loses 39% and 33% of its activity respectively, and at pH 10.5, the loss in enzyme activity is at 70%
additional information
-
wild-type and mutant enzymes, activity-pH relationships, profiles at different temperatures, overview