3.5.1.4: amidase

This is an abbreviated version!
For detailed information about amidase, go to the full flat file.

Reaction

Synonyms

4-guanidinobutyramide hydrolase, A-amidase, acetamidase, acid transferase, acylamidase, acylase, AMI1, amidase, amidase 1, amide hydrolase, amide transferase, amidohydrolase, AmiE, Ana, anandamide hydrolase, AtFAAH, Azl13, C-amidase, deaminase, ester transferase, FAAH, FAAH-1, FAAH-2, fatty acid amide hydrolase, fatty acid amide hydrolase 1, fatty acylamidase, half-amidase, indole-3-acetamide amidohydrolase, K1, mHG, Mhpg, More, murein peptide amidase A, mycothiol-S-conjugate amidase, N-acetylaminohydrolase, N-acylethanolamine acid amidase, N-acylethanolamine-hydrolyzing acid amidase, N-acylethanolaminehydrolyzing acid amidase, NAAA, NAE-hydrolyzing acid amidase, PamH, PYCH_10400, R-stereospecific amidase, RhAmidase, signature amidase, SsAH, SSAM, SSO2122, tissue kallikrein, Wide spectrum amidase

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.1 In linear amides

                3.5.1.4 amidase

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Results	in table
18	Activating Compound
23224	AA Sequence
38	Application
1	CAS Registry Number
37	Cloned(Commentary)
9	Crystallization (Commentary)
1520	Disease/ Diagnostics
15	General Information
1	General Stability
122	IC50 Value
650	Inhibitors
80	kcat/KM [mM/s]
272	Ki Value [mM]
192	KM Value [mM]
18	Localization
24	Metals/Ions
97	Molecular Weight [Da]
81	Natural Substrates/ Products (Substrates)
17	Organic Solvent Stability
455	Organism
2	Oxidation Stability
18	Pathway
52	PDB ID
55	pH Optimum
26	pH Range
10	pH Stability
5	pI Value
55	Protein Variants
58	Purification (Commentary)
9	Reaction
56	Reaction Type
194	Reference
2	Renatured (Commentary)
70	Source Tissue
135	Specific Activity [micromol/min/mg]
10	Storage Stability
572	Substrates and Products (Substrate)
77	Subunits
103	Synonyms
1	Systematic Name
49	Temperature Optimum [°C]
19	Temperature Range [°C]
40	Temperature Stability [°C]
124	Turnover Number [1/s]

Storage Stability

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of the Enzyme Summary Page.

Storage Stability on EC 3.5.1.4 - amidase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°C 0.35 M potassium phosphate with 0.5 mM DTT, 10 micromol CaCl2 and 15% glycerol, several weeks without loss of activity

Rhodococcus sp.

-

209108

-30°C, 0.05 M Tris buffer, pH 7.2, 50% v/v glycerol, 1 mM 2-mercaptoethanol

Pseudomonas aeruginosa

-

209123

4°C, purified enzyme, in 50 mM phosphate buffer, more than 6 months without loss of activity

Pseudonocardia thermophila

-

667278

4°C, the purified enzyme can be stored for longer than 6 months at in 50 mM phosphate buffer and 10% glycerol without any loss of activity

Pyrococcus yayanosii

F8AEP0

724942

glycerol 2%, v/v, stabilizes the enzyme, after storing three months at -18°C, 77% of the activity of purified enzyme recovered

Delftia acidovorans

-

209120

purified amidase stored in 0.05 M Tris buffer, pH 7.2, containing 1 mM mercaptoethanol and 50%, v/v, glycerol, at -30°C

Pseudomonas aeruginosa

-

209123

room temperature, half-life about 2000 days with ammonium sulfate

Rhodococcus erythropolis

-

209114

stable to freezing

Cucurbita maxima

-

209117

the enzyme may be stored at room temperature in the presence of 0.04% NaN3 for 1 year without any loss of activity

Saccharolobus solfataricus

P95896

719432

unstable even at 4°C

Agrobacterium tumefaciens

-

209117