3.4.22.B50: papain-like proteinase 2

This is an abbreviated version, for detailed information about papain-like proteinase 2, go to the full flat file.

Reaction

responsible for the cleavages located at the N-terminus of the replicase polyprotein =

Synonyms

Erv-C, ervatamin-C, mouse hepatitis virus papain-like proteinase 2, papain-like accessory proteases, papain-like cysteine proteinase, papain-like protease, papain-like protease domain 2, papain-like proteinase, PL2-PRO, PL2pro, PLP-2, PLP2, PLpro, SARS-coronavirus papain-like protease, SARS-CoV papain-like protease, SARS-CoV PLpro, severe acute respiratory syndrome coronavirus papain-like protease

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.B50 papain-like proteinase 2

Substrates Products

Substrates Products on EC 3.4.22.B50 - papain-like proteinase 2

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(E-EDANS)RELNGGAPI(K-DABCYL)S + H2O
?
show the reaction diagram
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
?
show the reaction diagram
azocasein + H2O
?
show the reaction diagram
-
-
-
?
BCoV substrate
?
show the reaction diagram
branched polyubiquitin chains + H2O
?
show the reaction diagram
diubiquitin + H2O
?
show the reaction diagram
FRLKGG-4-nitroanilide + H2O
FRLKGG + 4-nitroaniline
show the reaction diagram
-
-
-
?
FRLKGGAPIKGV
?
show the reaction diagram
FTKLAGGKISFS + H2O
FTKLAG + GKISFS
show the reaction diagram
-
-
-
-
?
Gelatin + H2O
?
show the reaction diagram
-
-
-
?
ISG15-nsp2-fusion protein + H2O
?
show the reaction diagram
ISLKGGKIVSTC
?
show the reaction diagram
MHV substrate
?
show the reaction diagram
-
-
-
-
?
N-benzoyl-Phe-Val-Arg-4-nitroanilide + H2O
N-benzoyl-Phe-Val-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
?
PLP2-MP1 precursor polyprotein + H2O
MP1 + ?
show the reaction diagram
-
-
-
-
?
polyubiquitin + H2O
monoubiquitin + ?
show the reaction diagram
the enzyme cleaves Lys48- and Lys63-linked polyubiquitin to monoubiquitin but not linear polyubiquitin
-
-
?
RELNGGAVTRYV + H2O
AVTYRV + RELNGG
show the reaction diagram
-
12 mer oligopeptide containing Gly180-Ala181, 5% cleavage
-
-
?
replicase polyprotein + H2O
?
show the reaction diagram
-
PLP2 cleaves a substrate encoding the first predicted membrane-spanning domain, MP1, of the replicase polyprotein. Processing the replicase polyprotein at this site generates the p150 replicase intermediate that is likely critical for embedding the replicase complex into cellular membranes. The enzyme acts efficiently in trans
-
-
?
RLRGG-7-amido-4-methylcoumarin + H2O
RLRGG + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
ubiquitin + H2O
?
show the reaction diagram
ubiquitin-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
ubiquitin-7-amido-4-trifluoromethylcoumarin + H2O
ubiquitin + 7-amino-4-trifluoromethylcoumarin
show the reaction diagram
the enzyme catalyzes proteolytic processing of the viral polyprotein and also shows significant in vitro deubiquitinating and de-ISGylating activities. The enzyme binds ubiquitin, the ubiquitin core makes mostly hydrophilic interactions with the enzyme, while the Leu-Arg-Gly-Gly C-terminus of ubiquitin is located in the catalytic cleft of the enzyme. The ubiquitin core binds to the palm, thumb and fingers domains of the enzyme, while its final four C-terminal residues bind into a narrow channel by a network of hydrogen bonds and reach towards the active site
-
-
?
ubiquitin-aminomethylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
ubiquitinated branched peptides + H2O
?
show the reaction diagram
-
-
-
-
?
ubiquitinated RIG-I + H2O
deubiquitinated RIG-I + ubiquitin
show the reaction diagram
-
-
-
?
ubiquitinated STING + H2O
deubiquitinated STING + ubiquitin
show the reaction diagram
-
-
-
?
VAKQGAGFKRTY + H2O
VAKQGA + GFKRTY
show the reaction diagram
-
-
-
-
?
viral replicase polyprotein + H2O
?
show the reaction diagram
-
PLP2 is responsible for processing both cleavage sites 2 and 3 to release nsp2 and nsp3. The cleavage sites are identified as FTKLAG-/-GKISFS for CS2 and VAKQGA-/-GFKRTY for CS3.
-
-
?
Z-KKAG-7-amido-4-methylcoumarin + H2O
Z-KKAG + 7-amino-4-methylcoumarin
show the reaction diagram
the catalytic efficiency toward Z-KKAG-7-amido-4-methylcoumarin is 5times higher than that for Z-LRGG-7-amido-4-methylcoumarin
-
-
?
Z-LRGG-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
Z-LRGG-7-amido-4-methylcoumarin + H2O
Z-LRGG + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
additional information
?
-