Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli XL-1 Blue and HeLa-MHV receptor cells | Alphacoronavirus |
Protein Variants | Comment | Organism |
---|---|---|
C1651A | purified SARS-CoV PLpro protein containing an alanine substitution at putative catalytic residues | Alphacoronavirus |
C1732A | encodes a substitution of a cysteine residue, predicted to be critical for zinc binding | Alphacoronavirus |
D1826A | purified SARS-CoV PLpro protein containing an alanine substitution at putative catalytic residues | Alphacoronavirus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
- |
Alphacoronavirus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alphacoronavirus | - |
SARS-CoV | - |
Alphacoronavirus SARS-CoV | - |
SARS-CoV | - |
Purification (Comment) | Organism |
---|---|
purified to homogeneity by hydrophobic interaction and anion exchange chromatography | Alphacoronavirus |
Storage Stability | Organism |
---|---|
-80°C, Tris buffer, pH 7.5, 20% glycerol | Alphacoronavirus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E-EDANS)RELNGGAPI(K-DABCYL)S + H2O | - |
Alphacoronavirus | ? | - |
? | |
(E-EDANS)RELNGGAPI(K-DABCYL)S + H2O | - |
Alphacoronavirus SARS-CoV | ? | - |
? | |
diubiquitin + H2O | - |
Alphacoronavirus | ? | - |
? | |
diubiquitin + H2O | - |
Alphacoronavirus SARS-CoV | ? | - |
? | |
ubiquitin-7-amido-4-methylcoumarin + H2O | - |
Alphacoronavirus | ? | - |
? | |
ubiquitin-7-amido-4-methylcoumarin + H2O | - |
Alphacoronavirus SARS-CoV | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
papain-like proteinase | - |
Alphacoronavirus |
PLpro | - |
Alphacoronavirus |