3.4.22.B50: papain-like proteinase 2
This is an abbreviated version!
For detailed information about papain-like proteinase 2, go to the full flat file.
Word Map on EC 3.4.22.B50
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3.4.22.B50
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polyproteins
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replicase
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coronaviruses
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3clpro
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isg15
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3c-like
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mhv-a59
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nsp3
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coronaviral
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pp1ab
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medicine
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drug development
- 3.4.22.B50
- polyproteins
-
replicase
- coronaviruses
- 3clpro
- isg15
-
3c-like
-
mhv-a59
- nsp3
- coronaviral
- pp1ab
- medicine
- drug development
Reaction
responsible for the cleavages located at the N-terminus of the replicase polyprotein =
Synonyms
Erv-C, ervatamin-C, mouse hepatitis virus papain-like proteinase 2, papain-like accessory protease, papain-like cysteine proteinase, papain-like protease, papain-like protease domain 2, papain-like proteinase, PL2-PRO, PL2pro, PLP-2, PLP2, PLpro, SARS-coronavirus papain-like protease, SARS-CoV papain-like protease, SARS-CoV PLpro, severe acute respiratory syndrome coronavirus papain-like protease
ECTree
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Purification
Purification on EC 3.4.22.B50 - papain-like proteinase 2
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GST resin column chromatography, Mono Q column chromatography, and Superdex 75 gel filtration
Ni-NTA column chromatography, Superdex 75 gel filtration, and Source 15Q column chromatography
purified to homogeneity by hydrophobic interaction and anion exchange chromatography
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recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3), the secretion tag is removed and the purification continued by nickel affinity chromatography, gel filtration, and ultrafiltration