3.4.22.B50: papain-like proteinase 2
This is an abbreviated version!
For detailed information about papain-like proteinase 2, go to the full flat file.
Word Map on EC 3.4.22.B50
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3.4.22.B50
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polyproteins
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replicase
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coronaviruses
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3clpro
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isg15
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3c-like
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mhv-a59
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nsp3
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coronaviral
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pp1ab
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medicine
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drug development
- 3.4.22.B50
- polyproteins
-
replicase
- coronaviruses
- 3clpro
- isg15
-
3c-like
-
mhv-a59
- nsp3
- coronaviral
- pp1ab
- medicine
- drug development
Reaction
responsible for the cleavages located at the N-terminus of the replicase polyprotein =
Synonyms
Erv-C, ervatamin-C, mouse hepatitis virus papain-like proteinase 2, papain-like accessory protease, papain-like cysteine proteinase, papain-like protease, papain-like protease domain 2, papain-like proteinase, PL2-PRO, PL2pro, PLP-2, PLP2, PLpro, SARS-coronavirus papain-like protease, SARS-CoV papain-like protease, SARS-CoV PLpro, severe acute respiratory syndrome coronavirus papain-like protease
ECTree
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Turnover Number
Turnover Number on EC 3.4.22.B50 - papain-like proteinase 2
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0.0041
mutant T39W, pH 6.5, 30°C
0.005
Dabcyl-FKKKGGGDVKE-Edans
wild-type, pH 6.5, 30°C
0.0011
wild-type, pH 6.5, 30°C
0.0175
Dabcyl-FRLKGGAPIKGV-Edans
mutant T39W, pH 6.5, 30°C
0.23
wild type enzyme, in 50 mM Na-acetate buffer pH 5.0, at 60°C
1.43
N-benzoyl-Phe-Val-Arg-4-nitroanilide
mutant enzyme S32T/A67Y, in 50 mM Na-acetate buffer pH 5.0, at 60°C
2.23
N-benzoyl-Phe-Val-Arg-4-nitroanilide
mutant enzyme S32T, in 50 mM Na-acetate buffer pH 5.0, at 60°C
2.65
N-benzoyl-Phe-Val-Arg-4-nitroanilide
mutant enzyme A67Y, in 50 mM Na-acetate buffer pH 5.0, at 60°C
0.01
recombinant mutant Y265A, pH 7.4, 30°C
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0.04
ubiquitin-7-amido-4-trifluoromethylcoumarin
recombinant mutant D165A, pH 7.4, 30°C
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0.09
ubiquitin-7-amido-4-trifluoromethylcoumarin
recombinant mutant L163Q, pH 7.4, 30°C
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0.29
ubiquitin-7-amido-4-trifluoromethylcoumarin
recombinant mutant E168A, pH 7.4, 30°C
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0.36
ubiquitin-7-amido-4-trifluoromethylcoumarin
recombinant mutant E168R, pH 7.4, 30°C
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0.61
ubiquitin-7-amido-4-trifluoromethylcoumarin
recombinant mutant E168D, pH 7.4, 30°C
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0.61
ubiquitin-7-amido-4-trifluoromethylcoumarin
recombinant mutant Y265F, pH 7.4, 30°C
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0.79
ubiquitin-7-amido-4-trifluoromethylcoumarin
recombinant wild-type enzyme, pH 7.4, 30°C
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