3.4.22.B50: papain-like proteinase 2
This is an abbreviated version!
For detailed information about papain-like proteinase 2, go to the full flat file.
Word Map on EC 3.4.22.B50
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3.4.22.B50
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polyproteins
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replicase
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coronaviruses
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3clpro
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isg15
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3c-like
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mhv-a59
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nsp3
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coronaviral
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pp1ab
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medicine
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drug development
- 3.4.22.B50
- polyproteins
-
replicase
- coronaviruses
- 3clpro
- isg15
-
3c-like
-
mhv-a59
- nsp3
- coronaviral
- pp1ab
- medicine
- drug development
Reaction
responsible for the cleavages located at the N-terminus of the replicase polyprotein =
Synonyms
Erv-C, ervatamin-C, mouse hepatitis virus papain-like proteinase 2, papain-like accessory protease, papain-like cysteine proteinase, papain-like protease, papain-like protease domain 2, papain-like proteinase, PL2-PRO, PL2pro, PLP-2, PLP2, PLpro, SARS-coronavirus papain-like protease, SARS-CoV papain-like protease, SARS-CoV PLpro, severe acute respiratory syndrome coronavirus papain-like protease
ECTree
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General Information
General Information on EC 3.4.22.B50 - papain-like proteinase 2
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malfunction
physiological function
additional information
arteriviruses lacking PLP2 deubiquitinase activity elicit an enhanced host innate immune response
malfunction
mutations of residues in the enzyme-ubiquitin interface lead to reduced catalytic activity
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papain-like protease domain 2 (PLP2) deubiquitinates TANK-binding kinase-1 (TBK1) and reduces its kinase activity, hence inhibits interferon-beta reporter activity and prevents interferon regulatory factor 3 (IRF3) nuclear translocation. The presence of PLP2 stabilizes the hypo-phosphorylated IRF3-TBK1 complex in a dose-dependent manner in the cytoplasm
physiological function
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PLP2 is responsible for the inhibition of both RIG-I and TLR3-dependent induction of interferon alpha/beta expression
physiological function
the enzyme is essential for arterivirus replication by cleaving a site within the viral replicase polyproteins and also removes ubiquitin from cellular proteins. This deubiquitinase activity is a critical factor in arteriviral innate immune evasion
physiological function
the enzyme is one of two cysteine proteases involved in the proteolytic processing of the polyproteins of Severe acute respiratory syndrome coronavirus. It also shows significant in vitro deubiquitinating and de-ISGylating activities
physiological function
the enzyme strongly inhibits RIG-Iand STING-activated interferon expression. Papain-like protease 2 acts as a viral deubiquitinase to interfere with the RIG-I- and STING-mediated signalling pathway
physiological function
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the primary function of the enzyme is to process the viral polyprotein in a coordinated manner. An additional function of the enzyme is stripping ubiquitin and ISG15 from host-cell proteins to aid the coronavirus in the evasion of the host innate immune responses, enzyme innate immune functions, overview
physiological function
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changes within the Ubl domain, residues 785 to 787 of nonstructural protein 3, negatively affect protease activity. Ubl mutant viruses V787S and V785S replicate efficiently at 37°C but generate smaller plaques than wild-type virus, and V787S is defective for replication at higher temperatures. The proteases of the mutant viruses exhibit similar specific activities at 25°C but diplay significantly reduced thermal stability at 30°C, thereby reducing the total enzymatic activity. Infection of C57BL/6 mice with V787S is highly attenuated, yet it replicates sufficiently to elicit protective immunity
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enzyme structure and function, active site structure with catalytic triad residues, Cys112, His273 and Asp287 and the oxyanion hole-stabilizing residue Trp107, and catalytic mechanism, detailed overview. The fingers domain of PLpro, which contains a zinc ion that is tetrahedrally coordinated by four cysteines, is essential for catalysis because it maintains the structural integrity of the enzyme
additional information
the enzyme comprises the viral polyprotein residues 1541-1858
additional information
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the enzyme comprises the viral polyprotein residues 1541-1858