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Literature summary for 3.4.22.B50 extracted from

  • Lindner, H.A.; Fotouhi-Ardakani, N.; Lytvyn, V.; Lachance, P.; Sulea, T.; Menard, R.
    The papain-like protease from the severe acute respiratory syndrome coronavirus is a deubiquitinating enzyme (2005), J. Virol., 79, 15199-15208.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine viral strategy to modulate the host cell ubiquitination machinery to its advantage Alphacoronavirus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) and HeLa cells Alphacoronavirus

Protein Variants

Protein Variants Comment Organism
C1651A Plpro active-site mutant Alphacoronavirus

Inhibitors

Inhibitors Comment Organism Structure
Ubiquitin aldehyde
-
Alphacoronavirus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
SDS-PAGE and Western blot detection with His6-specific antibodies, P-Plpro(C1)-His6 fragment Alphacoronavirus
41000
-
SDS-PAGE and Western blot detection with His6-specific antibodies, P-Plpro(C2)-His6 fragment Alphacoronavirus
60000
-
SDS-PAGE and Western blot detection with His6-specific antibodies, T7-ISG15P-Plpro(C2)-His6 fragment Alphacoronavirus

Organism

Organism UniProt Comment Textmining
Alphacoronavirus
-
SARS-CoV
-
Alphacoronavirus SARS-CoV
-
SARS-CoV
-

Purification (Commentary)

Purification (Comment) Organism
by His-Bind Purification Kit Alphacoronavirus

Storage Stability

Storage Stability Organism
-80°C, Tris-HCl buffer, pH 8.0, 10% glycerol, 1 month Alphacoronavirus
4°C, Tris-HCl buffer, pH 8.0, 10% glycerol, 24 h Alphacoronavirus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
branched polyubiquitin chains + H2O
-
Alphacoronavirus ?
-
?
branched polyubiquitin chains + H2O
-
Alphacoronavirus SARS-CoV ?
-
?
ISG15-nsp2-fusion protein + H2O
-
Alphacoronavirus ?
-
?
ISG15-nsp2-fusion protein + H2O
-
Alphacoronavirus SARS-CoV ?
-
?
ubiquitin-7-amido-4-methylcoumarin + H2O
-
Alphacoronavirus ?
-
?
ubiquitin-7-amido-4-methylcoumarin + H2O
-
Alphacoronavirus SARS-CoV ?
-
?
Z-LRGG-7-amido-4-methylcoumarin + H2O
-
Alphacoronavirus ?
-
?
Z-LRGG-7-amido-4-methylcoumarin + H2O
-
Alphacoronavirus SARS-CoV ?
-
?

Synonyms

Synonyms Comment Organism
papain-like proteinase
-
Alphacoronavirus
PLpro
-
Alphacoronavirus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00021
-
Ubiquitin aldehyde
-
Alphacoronavirus