Application | Comment | Organism |
---|---|---|
medicine | viral strategy to modulate the host cell ubiquitination machinery to its advantage | Alphacoronavirus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 (DE3) and HeLa cells | Alphacoronavirus |
Protein Variants | Comment | Organism |
---|---|---|
C1651A | Plpro active-site mutant | Alphacoronavirus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ubiquitin aldehyde | - |
Alphacoronavirus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
SDS-PAGE and Western blot detection with His6-specific antibodies, P-Plpro(C1)-His6 fragment | Alphacoronavirus |
41000 | - |
SDS-PAGE and Western blot detection with His6-specific antibodies, P-Plpro(C2)-His6 fragment | Alphacoronavirus |
60000 | - |
SDS-PAGE and Western blot detection with His6-specific antibodies, T7-ISG15P-Plpro(C2)-His6 fragment | Alphacoronavirus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alphacoronavirus | - |
SARS-CoV | - |
Alphacoronavirus SARS-CoV | - |
SARS-CoV | - |
Purification (Comment) | Organism |
---|---|
by His-Bind Purification Kit | Alphacoronavirus |
Storage Stability | Organism |
---|---|
-80°C, Tris-HCl buffer, pH 8.0, 10% glycerol, 1 month | Alphacoronavirus |
4°C, Tris-HCl buffer, pH 8.0, 10% glycerol, 24 h | Alphacoronavirus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
branched polyubiquitin chains + H2O | - |
Alphacoronavirus | ? | - |
? | |
branched polyubiquitin chains + H2O | - |
Alphacoronavirus SARS-CoV | ? | - |
? | |
ISG15-nsp2-fusion protein + H2O | - |
Alphacoronavirus | ? | - |
? | |
ISG15-nsp2-fusion protein + H2O | - |
Alphacoronavirus SARS-CoV | ? | - |
? | |
ubiquitin-7-amido-4-methylcoumarin + H2O | - |
Alphacoronavirus | ? | - |
? | |
ubiquitin-7-amido-4-methylcoumarin + H2O | - |
Alphacoronavirus SARS-CoV | ? | - |
? | |
Z-LRGG-7-amido-4-methylcoumarin + H2O | - |
Alphacoronavirus | ? | - |
? | |
Z-LRGG-7-amido-4-methylcoumarin + H2O | - |
Alphacoronavirus SARS-CoV | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
papain-like proteinase | - |
Alphacoronavirus |
PLpro | - |
Alphacoronavirus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00021 | - |
Ubiquitin aldehyde | - |
Alphacoronavirus |