Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | infectious bronchitis virus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 100 mM Bis-Tris, pH 6.5, 25-30% (w/v) polyethylene glycol monomethyl ether 2000 | infectious bronchitis virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.67 | - |
Z-LRGG-7-amido-4-methylcoumarin | in 20 mM Tris-HCl, pH 7.5, 0.1 mg/ml BSA, 150 mM NaCl, 2 mM dithiothreitol, at 25°C | infectious bronchitis virus | |
3.23 | - |
Z-KKAG-7-amido-4-methylcoumarin | in 20 mM Tris-HCl, pH 7.5, 0.1 mg/ml BSA, 150 mM NaCl, 2 mM dithiothreitol, at 25°C | infectious bronchitis virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
polyubiquitin + H2O | infectious bronchitis virus | the enzyme cleaves Lys48- and Lys63-linked polyubiquitin to monoubiquitin but not linear polyubiquitin | monoubiquitin + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
infectious bronchitis virus | P0C6Y1 | - |
- |
Purification (Comment) | Organism |
---|---|
GST resin column chromatography, Mono Q column chromatography, and Superdex 75 gel filtration | infectious bronchitis virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme cannot cleave Z-KAGG-7-amido-4-methylcoumarin | infectious bronchitis virus | ? | - |
? | |
polyubiquitin + H2O | the enzyme cleaves Lys48- and Lys63-linked polyubiquitin to monoubiquitin but not linear polyubiquitin | infectious bronchitis virus | monoubiquitin + ? | - |
? | |
Z-KKAG-7-amido-4-methylcoumarin + H2O | the catalytic efficiency toward Z-KKAG-7-amido-4-methylcoumarin is 5times higher than that for Z-LRGG-7-amido-4-methylcoumarin | infectious bronchitis virus | Z-KKAG + 7-amino-4-methylcoumarin | - |
? | |
Z-LRGG-7-amido-4-methylcoumarin + H2O | - |
infectious bronchitis virus | Z-LRGG + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PLpro | - |
infectious bronchitis virus |