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Literature summary for 3.4.22.B50 extracted from

  • Han, Y.S.; Chang, G.G.; Juo, C.G.; Lee, H.J.; Yeh, S.H.; Hsu, J.T.; Chen, X.
    Papain-like protease 2 (PLP2) from severe acute respiratory syndrome coronavirus (SARS-CoV): expression, purification, characterization, and inhibition (2005), Biochemistry, 44, 10349-10359.
    View publication on PubMed

Application

Application Comment Organism
medicine antiviral therapy Alphacoronavirus

Cloned(Commentary)

Cloned (Comment) Organism
cloned into modified version of the pBacPAK8/His2 vector and expressed in insect cells Sf9 Alphacoronavirus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ weakly inhibited by 70% at 0.01 mM Alphacoronavirus
hydroxypridine-2-thione Zn
-
Alphacoronavirus
additional information not inhibited by Mg2+, Mn2+, Ca2+, Ni2+ and Co2+ at 0.01 mM, E64, leupeptin and antipain at 0.1 mM, N-ethylmaleimide and chymostatin at 1 mM and cystatin at 0.01 mg/mL respectively Alphacoronavirus
N-ethyl-N-phenyldithiocarbaic acid Zn
-
Alphacoronavirus
Zn2+
-
Alphacoronavirus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 5.25 Alphacoronavirus
0.064
-
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 6.05 Alphacoronavirus
0.0654
-
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 6.82 Alphacoronavirus
0.068
-
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 9.2 Alphacoronavirus
0.0704
-
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 9.8 Alphacoronavirus
0.0714
-
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 8.65 Alphacoronavirus
0.072
-
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 8.01 Alphacoronavirus
0.0772
-
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 7.42 Alphacoronavirus
0.185
-
FRLKGGAPIKGV
-
Alphacoronavirus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
SDS-PAGE Alphacoronavirus
56700
-
gel filtration Alphacoronavirus

Organism

Organism UniProt Comment Textmining
Alphacoronavirus
-
SARS-CoV
-
Alphacoronavirus SARS-CoV
-
SARS-CoV
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration Alphacoronavirus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine + H2O fluorogenic substrate Alphacoronavirus ?
-
?
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine + H2O fluorogenic substrate Alphacoronavirus SARS-CoV ?
-
?
BCoV substrate
-
Alphacoronavirus ?
-
?
BCoV substrate
-
Alphacoronavirus SARS-CoV ?
-
?
FRLKGGAPIKGV 12 mer oligopeptide containing Gly818-Ala819, 100% cleavage Alphacoronavirus ?
-
?
FRLKGGAPIKGV 12 mer oligopeptide containing Gly818-Ala819, 100% cleavage Alphacoronavirus SARS-CoV ?
-
?
ISLKGGKIVSTC 12 mer oligopeptide containing Gly2740-Lys2741, 29% cleavage Alphacoronavirus ?
-
?
ISLKGGKIVSTC 12 mer oligopeptide containing Gly2740-Lys2741, 29% cleavage Alphacoronavirus SARS-CoV ?
-
?
MHV substrate
-
Alphacoronavirus ?
-
?
additional information SARS-CoV PLP2 does not cleave HCoV.229E and IBV substrates Alphacoronavirus ?
-
?
additional information SARS-CoV PLP2 does not cleave HCoV.229E and IBV substrates Alphacoronavirus SARS-CoV ?
-
?
RELNGGAVTRYV + H2O 12 mer oligopeptide containing Gly180-Ala181, 5% cleavage Alphacoronavirus AVTYRV + RELNGG
-
?

Subunits

Subunits Comment Organism
monomer
-
Alphacoronavirus

Synonyms

Synonyms Comment Organism
papain-like protease
-
Alphacoronavirus
PLP2
-
Alphacoronavirus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
thermolabil, significantly reduced activity after incubation at temperatures higher than 37°C for 30 min Alphacoronavirus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.023
-
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 5.25 Alphacoronavirus
0.025
-
FRLKGGAPIKGV
-
Alphacoronavirus
0.1
-
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 9.8 Alphacoronavirus
0.198
-
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 6.05 Alphacoronavirus
0.233
-
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 9.2 Alphacoronavirus
0.33
-
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 7.42 Alphacoronavirus
0.343
-
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 6.82 Alphacoronavirus
0.363
-
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 8.01 Alphacoronavirus
0.366
-
Abz-FRLKGGAPIKGV-N-(2,4-dinitrophenyl)-ethylenediamine at pH 8.65 Alphacoronavirus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 8.6
-
Alphacoronavirus