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3.2.1.55: non-reducing end alpha-L-arabinofuranosidase

This is an abbreviated version!
For detailed information about non-reducing end alpha-L-arabinofuranosidase, go to the full flat file.

Word Map on EC 3.2.1.55

Reaction

1,5-alpha-L-arabinofuranohexaose
+ 5 H2O = 6 alpha-L-arabinofuranose

Synonyms

ABF, ABF 2, Abf II, Abf III, ABF1, Abf2, ABF3, Abf4, Abf43A, Abf5, AbF51, Abf51A, Abf51B, Abf6, Abf62A, Abf62A-Axe6A, Abf62A-m2,3, AbfA, AbfATK4, AbfB, AbfD3, AbfI, AbfII, AbjA, acid arabinosidase, AF, AF2, AFase, Afase I, Afase II, AFQ, AFQ1, AFS, AFS1, AfuB, AkAbf, AkAbf54, AkabfA, AkAbfB, alpha-1,3-arabinofuranosidase, alpha-arabinofuranosidase, alpha-arabinofuranosidase B, alpha-arabinosidase, alpha-AraF, alpha-L-Abfase, Alpha-L-AF, alpha-L-Afase, alpha-L-arabinanase, alpha-L-arabinofuranosidase, alpha-L-arabinofuranosidase 54, alpha-L-arabinofuranosidase B, alpha-L-arabinofuranosidase D3, alpha-L-arabinofuranosidase II, alpha-L-arabinofuranosidase III, alpha-L-arabinofuranosidase/beta-D-xylosidase, alpha-L-arabinofuranoside arabinofuranohydrolase, alpha-L-arabinofuranoside hydrolase, alpha-L-arabinosidase, alpha-L-Araf, alpha-L-arafase, alpha-L-arafase I, alpha-L-arafase II, AN7908, AoAra54A, ARA-I, AraB, arabinofuranosidase, arabinofuranosidase 3, arabinofuranosidase 43A, arabinofuranosyl hydrolase, arabinosidase, arabinoxylan alpha-L-1,3-arabinofuranohydrolase, arabinoxylan alpha-L-arabinofuranohydrolase, arabinoxylan arabinofuranohydrolase, arabinoxylan hydrolase, AraF, Araf 51, Araf-ase, AraF43, Araf43A, Araf51A, Arb43A, Arb93A, ARF, Arfase, AtBXL1, AvAbfB, AXH, AXH-d3, Axh43A, Axh43B, AXS5, Axy43A, beta-D-galactofuranosidase 1, beta-D-galactofuranosidase 3, beta-D-galactofuranosidase 4, beta-D-xylosidase/alpha-L-arabinofuranosidase, beta-D-xylosidase/alpha-L-arabinosidase, beta-xylosidase/alpha-arabinofuranosidase, beta-xylosidase/alpha-arabinosidase, BlAraf, blArafA, BsAXH-m2,3, CcAbf62A, ccxyl3a, Csac_2411, Ct43Araf, CtCBM6B, CtGH43, EC 3.2.1.79, exo-1,5-alpha-L-arabinanase, exo-1,5-alpha-L-arabinofuranosidase, exo-alpha-L-arabinanase, exo-alpha-L-arabinofuranosidase, exo-arabinanase 43A, family 54 alpha-L-arabinofuranosidase, Galf-ase, GH 51 Abf, GH family 54 alpha-L-arabinofuranosidase, GH43 alpha-L-arabinofuranosidase, GH43B6, GH43_22 alpha-L-arabinofuranosidase, GH51 alpha-arabinofuranosidase, GH51 alpha-L-arabinofuranosidase, GH51 arabinofuranosidase, GH54 alpha-L-arabinofuranosidase, GH62, GH62 alpha-L-arabinofuranosidase, GRMZM2G077299, GRMZM2G120132, GRMZM2G180889, GRMZM2G393002, Hore_20580, L-arabinofuranosidase, L-arabinosidase, L213A variant beta-glycosidase, More, ORF0232, ORF2125, ORF2812, PaAbf62A, PcABF43A, PfABF62a, PfABF62b, PfABF62c, PoAbf, polysaccharide alpha-L-arabinofuranosidase, rArfA, RuXyn1, ScAraf62A, STX-IV, swu62A, Theth_1120, TmAFase, Tx-Abf, UmAbf62A, xarB, XYL3, XylC, XynD, XynF

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.55 non-reducing end alpha-L-arabinofuranosidase

Purification

Purification on EC 3.2.1.55 - non-reducing end alpha-L-arabinofuranosidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
1080fold from 5 days old seedlings
16.94fold from culture supernatant by ammonium sulfate precipitation and chromatographical methods
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2 isoenzymes
2 isoenzymes, alpha-L-arafase I and alpha-L-arafase II
-
2 isoenzymes, C1and C2
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3 isoenzymes, Ara1, Ara2 and Ara3
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30 min heat treatment at 80°C, hydrophobic interaction, anion exchange and gel permeation column chromatography
-
59fold to homogeneity by ammonium sulfate precipitation and 4 chromatographic steps
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ABF1, 7.9fold, and ABF2, 20.4fold, to homogeneity
-
ammonium sulfate precipitation and phenyl Sepharose 6 column chromatography
-
ammonium sulfate precipitation, DEAE-Sephadex gel filtration, SP Sepharose column chromatography and BioGel P-300 gel filtration
ammonium sulfate precipitation, Mono Q column chromatography, and Mono S column chromatography. Ni-Sepharose 6 column chromatography
ammonium sulfate precipitation, Q-sepharose column chromatography, Sephacryl S-200 gel filtration and Mono Q column chromatography
-
anion exchange and gel filtration
-
both type B enzymes AFQ1, 17fold, and AFS1, 39.7fold, from culture filtrate
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column chromatography
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DEAE exchange chromatography and HQ/20 cation exchange chromatography
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flash chromatography on silica gel columns
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gel filtration
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GH43 alpha-L-arabinofuranosidase
-
GH51 alpha-L-arabinofuranosidase
heat treatment and Hi-Trap anion exchange column chromatography
-
His-tag nickel affinity column chromatography
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His-Trap column chromatography
HisTrap column chromatography
hydroxyapatite, anion exchange chromatography, size-exclusion chromatography
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immobilized metal affinity column chromatography, and Superdex 75 gel filtration
isoenzyme designated AFIII
-
isoenzymes AF35 and AF53
-
MagneHisTM Protein Purification System
method not mentioned
-
native extracellular enzyme 1030fold to homogeneity
native extracellular enzyme 7.7fold from Penicillium funiculosum culture medium supernatant by ultrafiltration, ammonium sulfate fractionation, dialysis, two different steps of anion exchange chromatography, and hydrophobic interaction chromatography, to homogeneity
Ni-affinity column chromatography and Superdex 75 gel filtration
Ni-nitrilotriacetic acid (NTA) agarose column chromatography, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
Ni-NTA agarose affinity chromatography
Ni-NTA agarose column chromatography
-
Ni-NTA column chromatography
Ni-NTA column chromatography and HiTrap Q column chromatography
Ni-NTA resin column chromatography
Ni-NTA resin column chromatography and Uno-Q column chromatography
Ni2+-charged HiTrap Chelating HP column chromatography and Superdex 200 gel filtration
Ni2+-charged HiTrap IMAC column chromatography
nickel column chromatography
-
nickel-affinity column chromatography and gel filtration
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partially
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precipitation followed by column chromatography
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precipitation, chromatography in a DEAESephadex A 25, cation exchange chromatography in SP Sepharose
recombinant
recombinant C-terminally His-tagged enzyme without the N-terminal signal peptide from Escherichia coli strain BL21(DE3)
recombinant enzyme
recombinant enzyme 25.6fold from Escherichia coli by heat treatment and anion exchange chromatography
recombinant enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant enzyme, expressed in Escherichia coli
-
recombinant enzyme, expressed in Escherichia coli BL21(DE3)
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recombinant extracellular enzyme from Pichia pastoris culture supernatant
recombinant His-tagged enzyme 75fold from Aspergillus vadensis strain CBS 113365 culture supernatant
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recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by immobilized metal affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain MC1061 by nickel affinity chromatography, dialysis, and gel filtration, to homogeneity
recombinant His-tagged enzyme from Lactococcus lactis by Ni2+-chelate affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain TOP10 by metal affinity and anion exchange chromatography
-
recombinant His6-tagged enzyme 7.4fold from Escherichia coli by nickel affinity chromatography to homogeneity
recombinant His6-tagged enzyme from Pichia pastoris strain X33
recombinant His6-tagged truncated enzyme from Escherichia coli strain BL21(DE3) pLysS by nickel affinity chromatography and gel filtration
recombinant His6-tagged wild-type isozymes and isozyme pfabf62c mutant pfabf62cDELTACBM from Pichia pastoris strain GS115 by immobilized metal affinity chromatography
-
recombinant isozyme AFQ1 from Escherichia coli culture supernatant
recombinant isozyme AFS1 from Escherichia coli culture supernatant
recombinant N-termially 2 x His6- and Nus-tagged enzyme from Escherichia coli strain BL21(DE3)CodonPlus, by nickel affinity chromatography, tag cleavage by HRV3C protease, followed by nickel affinity chromatography and ultrafiltration
recombinant N-terminally 2 x His6- and Nus-tagged enzyme from Escherichia coli strain BL21(DE3)CodonPlus, by nickel affinity chromatography, tag cleavage by HRV3C protease, followed by nickel affinity chromatography and ultrafiltration
recombinant protein, arfB gene product
-
recombinant protein, expressed in Escherichia coli
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recombinant wild-type and mutant enzymes from Escherichia coli
recombinant wild-type and mutant enzymes from Streptomyces lividans strain 1326 culture medium by affinity chromatography using lactosyl-Sepharose, dialysis and ultrafiltration
sulfopropyl Sepharose column chromatography (isoform AbfI) and hydroxyapatite column chromatography (isoform AbfII)
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to homogeneity from cell culture supernatant
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