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Literature summary for 3.2.1.55 extracted from

  • Yan, Q.; Tang, L.; Yang, S.; Zhou, P.; Zhang, S.; Jiang, Z.
    Purification and characterization of a novel thermostable alpha-L-arabinofuranosidase (alpha-L-AFase) from Chaetomium sp. (2012), Process Biochem., 47, 472-478.
No PubMed abstract available
Search for more literature for 3.2.1.55

Cloned(Commentary)

Cloned (Comment) Organism
gene CsAra, DNA and amino acid sequence determination and analysis, sequence comparisons Chaetomium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.43
-
 4-nitrophenyl-alpha-L-arabinofuranoside native enzyme, pH 5.0, 50°C Chaetomium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Chaetomium sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51600
-
 gel filtration Chaetomium sp.
52900
-
 1 * 52900, SDS-PAGE Chaetomium sp.

Organism

Organism UniProt Comment Textmining
Chaetomium sp. K7QK60 gene CsAra
-
Chaetomium sp. CQ31 K7QK60 gene CsAra
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 1030fold to homogeneity Chaetomium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.6
-
 purified native enzyme, pH 5.0, 50°C Chaetomium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Chaetomium sp. 4-nitrophenol + L-arabinofuranose
-
 ?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Chaetomium sp. CQ31 4-nitrophenol + L-arabinofuranose
-
 ?
additional information the enzyme shows strict substrate specificity for the alpha-L-arabinofuranosyl linkage Chaetomium sp. ?
-
 ?
additional information the enzyme shows strict substrate specificity for the alpha-L-arabinofuranosyl linkage Chaetomium sp. CQ31 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 52900, SDS-PAGE Chaetomium sp.

Synonyms

Synonyms Comment Organism
alpha-L-Afase
-
 Chaetomium sp.
alpha-L-arabinofuranosidase
-
 Chaetomium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Chaetomium sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 85 activity range, profile overview Chaetomium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55 70 purified native enzyme, stable up to 55°C for 30 min, half-lives at 55°C, 60°C, 65°C and 70°C are 167.4 min, 104.6 min, 28.1 min and 10.3 min, respectively Chaetomium sp.
60
-
 purified native enzyme, 1 h, 75% activity remaining Chaetomium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Chaetomium sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 10 activity range, broad optimal activity ranging with high activity at pH 4.5-7.0, the enzyme activity decreases sharply when the pH increases above pH 8.0 or decreased below pH 4.0, profile overview Chaetomium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10 purified native enzyme, completely stable at Chaetomium sp.

General Information

General Information Comment Organism
evolution the enzyme belongs to glycosyl hydrolase family 43, GH43 Chaetomium sp.