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Literature summary for 3.2.1.55 extracted from
- Purification and characterization of an alpha-L-arabinofuranosidase from Clostridium acetobutylicum ATCC 824 (1987), Can. J. Microbiol., 33, 1011-1016.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.082 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Streptomyces purpurascens |  |
| 0.26 | - |
beet arabinan | - |
Aspergillus niger | |
| 1.6 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Ruminococcus albus | |
| 2 | - |
beet arabinan | - |
Clostridium acetobutylicum | |
| 3.6 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Streptomyces sp. | |
| 4 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Ruminococcus albus | |
| 4 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Clostridium acetobutylicum | |
| 4.9 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Aspergillus niger | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aspergillus niger | - |
- |
| extracellular | - |
Ruminococcus albus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
- |
Aspergillus niger |
| 62000 | - |
8 * 62000 | Streptomyces purpurascens |
| 65000 | - |
- |
Bacillus subtilis |
| 75000 | - |
4 * 75000, SDS-PAGE | Ruminococcus albus |
| 90000 | - |
SDS-PAGE | Clostridium acetobutylicum |
| 92000 | - |
- |
Streptomyces sp. |
| 94000 | - |
gel filtration | Clostridium acetobutylicum |
| 310000 | - |
- |
Ruminococcus albus |
| 495000 | - |
- |
Streptomyces purpurascens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beet arabinan + H2O | Bacillus subtilis | - |
L-arabinose + ? | - |
ir | |
| beet arabinan + H2O | Aspergillus niger | - |
L-arabinose + ? | - |
ir | |
| beet arabinan + H2O | Ruminococcus albus | - |
L-arabinose + ? | - |
ir | |
| beet arabinan + H2O | Streptomyces sp. | - |
L-arabinose + ? | - |
ir | |
| beet arabinan + H2O | Clostridium acetobutylicum | - |
L-arabinose + ? | - |
ir | |
| beet arabinan + H2O | Streptomyces purpurascens | - |
L-arabinose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
| Bacillus subtilis | - |
- |
- |
| Clostridium acetobutylicum | - |
ATCC 824 | - |
| Ruminococcus albus | - |
- |
- |
| Streptomyces purpurascens | - |
- |
- |
| Streptomyces sp. | - |
no. 17-1 | - |
| Streptomyces sp. 17-1 | - |
no. 17-1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
- |
Ruminococcus albus |
- |
Clostridium acetobutylicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.8 | - |
p-nitrophenyl-alpha-D-glucoside as substrate | Clostridium acetobutylicum |
| 19.2 | - |
- |
Clostridium acetobutylicum |
| 19.4 | - |
p-nitrophenyl-alpha-L-arabinofuranoside as substrate | Clostridium acetobutylicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,5-alpha-L-arabinobiose + H2O | - |
Streptomyces purpurascens | L-arabinose | - |
? | |
| 1,5-alpha-L-arabinotriose + 2 H2O | - |
Aspergillus niger | 3 L-arabinose | - |
? | |
| 1,5-alpha-L-arabinotriose + 2 H2O | - |
Streptomyces purpurascens | 3 L-arabinose | - |
? | |
| 4-nitrophenyl alpha-D-glucoside + H2O | 9% of the rate exhibited on p-nitrophenyl-alpha-L-arabinofuranoside | Clostridium acetobutylicum | 4-nitrophenol + D-glucose | - |
? | |
| base-soluble carbohydrates + H2O | BSC of alfalfa cell walls | Ruminococcus albus | ? | - |
? | |
| beet arabinan + H2O | - |
Bacillus subtilis | L-arabinose + ? | - |
ir | |
| beet arabinan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
ir | |
| beet arabinan + H2O | - |
Ruminococcus albus | L-arabinose + ? | - |
ir | |
| beet arabinan + H2O | - |
Streptomyces sp. | L-arabinose + ? | - |
ir | |
| beet arabinan + H2O | - |
Clostridium acetobutylicum | L-arabinose + ? | - |
ir | |
| beet arabinan + H2O | - |
Streptomyces purpurascens | L-arabinose + ? | - |
? | |
| L-arabinan + H2O | - |
Bacillus subtilis | (1,5-alpha-L-arabinose)25 + ? | - |
ir | |
| L-arabinan + H2O | - |
Aspergillus niger | (1,5-alpha-L-arabinose)25 + ? | - |
ir | |
| L-arabinan + H2O | - |
Clostridium acetobutylicum | (1,5-alpha-L-arabinose)25 + ? | - |
ir | |
| L-arabinan + H2O | - |
Streptomyces purpurascens | (1,5-alpha-L-arabinose)25 + ? | - |
? | |
| L-arabinogalactan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
? | |
| L-arabinogalactan + H2O | - |
Streptomyces sp. | L-arabinose + ? | - |
? | |
| L-arabinogalactan + H2O | - |
Streptomyces sp. 17-1 | L-arabinose + ? | - |
? | |
| L-arabinoxylan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
ir | |
| L-arabinoxylan + H2O | - |
Streptomyces sp. | L-arabinose + ? | - |
ir | |
| L-arabinoxylan + H2O | - |
Streptomyces sp. 17-1 | L-arabinose + ? | - |
ir | |
| additional information | - |
Bacillus subtilis | ? | - |
? | |
| additional information | - |
Aspergillus niger | ? | - |
? | |
| additional information | - |
Streptomyces sp. | ? | - |
? | |
| additional information | - |
Streptomyces purpurascens | ? | - |
? | |
| additional information | no activity against p-nitrophenylglycosides, except p-nitrophenyl-alpha-D-glucoside, no activity on carboxymethylcellulose, arabinogalactan, arabinoxylan or oat spelt xylan, larchwood xylan, CMC and rye arabinoxylan are not degraded | Clostridium acetobutylicum | ? | - |
? | |
| additional information | - |
Streptomyces sp. 17-1 | ? | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Bacillus subtilis | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Ruminococcus albus | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Streptomyces sp. | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Clostridium acetobutylicum | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Streptomyces purpurascens | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Streptomyces sp. 17-1 | p-nitrophenol + L-arabinose | - |
? | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Bacillus subtilis | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Ruminococcus albus | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces sp. | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Clostridium acetobutylicum | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces purpurascens | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces sp. 17-1 | L-arabinose + phenol | - |
ir | |
| polygalacturonase-soluble carbohydrates + H2O | PGSC of alfalfa cell walls | Ruminococcus albus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Bacillus subtilis |
| monomer | - |
Aspergillus niger |
| monomer | - |
Streptomyces sp. |
| monomer | 1 * 90000, SDS-PAGE | Clostridium acetobutylicum |
| octamer | 8 * 62000 | Streptomyces purpurascens |
| tetramer | 4 * 75000, SDS-PAGE | Ruminococcus albus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.8 | 4 | - |
Aspergillus niger |
| 5 | 5.5 | - |
Clostridium acetobutylicum |
| 6 | - |
- |
Streptomyces sp. |
| 6.5 | - |
- |
Bacillus subtilis |
| 6.5 | - |
- |
Streptomyces purpurascens |
| 6.9 | - |
- |
Ruminococcus albus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 4 | poor stability at low pH | Clostridium acetobutylicum |
| 4 | 9 | - |
Streptomyces sp. |
| 5.5 | 8 | - |
Clostridium acetobutylicum |
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