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Literature summary for 3.2.1.55 extracted from

  • de Wet, B.J.; Matthew, M.K.; Storbeck, K.; van Zyl, W.H.; Prior, B.A.
    Characterization of a family 54 alpha -L-arabinofuranosidase from Aureobasidium pullulans (2008), Appl. Microbiol. Biotechnol., 77, 975-983.
    View publication on PubMed
Search for more literature for 3.2.1.55

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Aureobasidium pullulans

Inhibitors

Inhibitors Comment Organism Structure
arabinose noncompetitive inhibitor, 56% inhibition at 50 mM Aureobasidium pullulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7
-
 p-nitrophenyl-alpha-L-arabinofuranoside
-
 Aureobasidium pullulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 gel filtration Aureobasidium pullulans
49000
-
 1 * 49000, SDS-PAGE Aureobasidium pullulans
49900
-
 1 * 49900, calculated from sequence Aureobasidium pullulans

Organism

Organism UniProt Comment Textmining
Aureobasidium pullulans Q6RKQ1
-
-
Aureobasidium pullulans NRRL Y-2311-1 Q6RKQ1
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Aureobasidium pullulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-1,5-L-arabinopentaose + H2O low activity Aureobasidium pullulans L-arabinose + ?
-
 ?
alpha-1,5-L-arabinopentaose + H2O low activity Aureobasidium pullulans NRRL Y-2311-1 L-arabinose + ?
-
 ?
alpha-1,5-L-arabinotetraose + H2O low activity Aureobasidium pullulans L-arabinose + ?
-
 ?
alpha-1,5-L-arabinotetraose + H2O low activity Aureobasidium pullulans NRRL Y-2311-1 L-arabinose + ?
-
 ?
alpha-1,5-L-arabinotriose + H2O low activity Aureobasidium pullulans L-arabinose + ?
-
 ?
alpha-1,5-L-arabinotriose + H2O low activity Aureobasidium pullulans NRRL Y-2311-1 L-arabinose + ?
-
 ?
arabinoxylan + H2O the enzyme releases arabinose from maize fiber, oat spelt arabinoxylan, and wheat arabinoxylan, but not from larch wood arabinogalactan or alpha-1,5-debranched arabinan Aureobasidium pullulans ?
-
 ?
arabinoxylan + H2O the enzyme releases arabinose from maize fiber, oat spelt arabinoxylan, and wheat arabinoxylan, but not from larch wood arabinogalactan or alpha-1,5-debranched arabinan Aureobasidium pullulans NRRL Y-2311-1 ?
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Aureobasidium pullulans p-nitrophenol + L-arabinofuranose
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Aureobasidium pullulans NRRL Y-2311-1 p-nitrophenol + L-arabinofuranose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 49000, SDS-PAGE Aureobasidium pullulans
monomer 1 * 49900, calculated from sequence Aureobasidium pullulans

Synonyms

Synonyms Comment Organism
AkabfA
-
 Aureobasidium pullulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Aureobasidium pullulans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 60 45°C: about 55% of maximal activity, 60°C: about 70% of maximal activity Aureobasidium pullulans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 2 h, stable Aureobasidium pullulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5 4
-
 Aureobasidium pullulans

pH Range

pH Minimum pH Maximum Comment Organism
2 5 pH 2.0: about 65% of maximal activity, pH 5.0: about 50% of maximal activity Aureobasidium pullulans

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.2
-
 15 min, complete inactivation Aureobasidium pullulans
3
-
 half-life: 29 min Aureobasidium pullulans
4 5 2 h, stable Aureobasidium pullulans
6
-
 15 min, complete inactivation Aureobasidium pullulans

pI Value

Organism Comment pI Value Maximum pI Value
Aureobasidium pullulans calculated from sequence
-
 4.97