Application | Comment | Organism |
---|---|---|
analysis | - |
Aspergillus niger |
analysis | enzymatic tool for structural analysis of oligo- and polysaccharides | Thermoclostridium stercorarium |
degradation | application potential for industrial purposes, biomass degradation and refining, extremely low end-product inhibition by arabinose further increases its applicability | Thermoclostridium stercorarium |
Cloned (Comment) | Organism |
---|---|
gene, encoding AF, heterologously expressed in Escherichia coli JM83 | Thermoclostridium stercorarium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Thermoclostridium stercorarium | |
ethanol | slight inhibition | Thermoclostridium stercorarium | |
Hg2+ | - |
Geobacillus stearothermophilus | |
Hg2+ | - |
Streptomyces purpurascens | |
Hg2+ | - |
Thermoclostridium stercorarium | |
p-hydroxymercuribenzoate | - |
Thermoclostridium stercorarium | |
SDS | - |
Thermoclostridium stercorarium | |
Zn2+ | - |
Thermoclostridium stercorarium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Geobacillus stearothermophilus | - |
- |
extracellular | - |
Aspergillus niger | - |
- |
extracellular | - |
Ruminococcus albus | - |
- |
extracellular | - |
Thermoclostridium stercorarium | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
zymogram staining | Thermoclostridium stercorarium |
75000 | - |
4 * 75000, SDS-PAGE | Ruminococcus albus |
195000 | - |
gel filtration | Thermoclostridium stercorarium |
310000 | - |
- |
Ruminococcus albus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oat spelt xylan + H2O | Geobacillus stearothermophilus | - |
L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | Aspergillus niger | - |
L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | Butyrivibrio fibrisolvens | - |
L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | Thermoclostridium stercorarium | - |
L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | Butyrivibrio fibrisolvens GS113 | - |
L-arabinose + ? | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | resistance to ethanol, neutral detergents and NaCl | Thermoclostridium stercorarium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Butyrivibrio fibrisolvens | - |
GS113 | - |
Butyrivibrio fibrisolvens GS113 | - |
GS113 | - |
Geobacillus stearothermophilus | - |
- |
- |
Ruminococcus albus | - |
8 | - |
Ruminococcus albus 8 | - |
8 | - |
Streptomyces purpurascens | - |
- |
- |
Thermoclostridium stercorarium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Ruminococcus albus |
recombinant protein, arfB gene product | Thermoclostridium stercorarium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
883 | - |
recombinant arfB gene product | Thermoclostridium stercorarium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | 0.26% of activity observed with p-nitrophenyl-alpha-L-arabinofuranoside | Thermoclostridium stercorarium | 4-nitrophenol + D-glucopyranose | - |
? | |
alkyl-alpha-arabinofuranoside + H2O | - |
Thermoclostridium stercorarium | L-arabinose + alkyl alcohol | - |
? | |
aryl-alpha-arabinofuranoside + H2O | - |
Thermoclostridium stercorarium | L-arabinose + aryl alcohol | - |
? | |
L-arabinogalactan + H2O | - |
Geobacillus stearothermophilus | L-arabinose + ? | - |
? | |
L-arabinogalactan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
? | |
L-arabinogalactan + H2O | - |
Thermoclostridium stercorarium | L-arabinose + ? | - |
? | |
L-arabinoxylan + H2O | - |
Geobacillus stearothermophilus | L-arabinose + ? | - |
ir | |
L-arabinoxylan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
ir | |
L-arabinoxylan + H2O | - |
Thermoclostridium stercorarium | L-arabinose + ? | - |
ir | |
methylumbelliferyl-alpha-L-arabinofuranoside + H2O | - |
Butyrivibrio fibrisolvens | methylumbelliferone | - |
? | |
methylumbelliferyl-alpha-L-arabinofuranoside + H2O | - |
Thermoclostridium stercorarium | methylumbelliferone | - |
? | |
methylumbelliferyl-alpha-L-arabinofuranoside + H2O | - |
Butyrivibrio fibrisolvens GS113 | methylumbelliferone | - |
? | |
oat spelt xylan + H2O | - |
Geobacillus stearothermophilus | L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | - |
Butyrivibrio fibrisolvens | L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | - |
Thermoclostridium stercorarium | L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | - |
Butyrivibrio fibrisolvens GS113 | L-arabinose + ? | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Geobacillus stearothermophilus | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Ruminococcus albus | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Thermoclostridium stercorarium | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Streptomyces purpurascens | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Ruminococcus albus 8 | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-beta-D-galactopyranoside + H2O | 0.33% of activity observed with p-nitrophenyl-alpha-L-arabinofuranoside | Thermoclostridium stercorarium | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
p-nitrophenyl-beta-D-xylopyranoside + H2O | 0.44% of activity observed with p-nitrophenyl-alpha-L-arabinofuranoside | Thermoclostridium stercorarium | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Geobacillus stearothermophilus | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Ruminococcus albus | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Butyrivibrio fibrisolvens | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Thermoclostridium stercorarium | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces purpurascens | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Ruminococcus albus 8 | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Butyrivibrio fibrisolvens GS113 | L-arabinose + phenol | - |
ir |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Geobacillus stearothermophilus |
tetramer | 4 * 52000, SDS-PAGE | Thermoclostridium stercorarium |
tetramer | 4 * 75000, SDS-PAGE | Ruminococcus albus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Geobacillus stearothermophilus |
70 | - |
- |
Thermoclostridium stercorarium |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 90 | - |
Thermoclostridium stercorarium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 90 | remarkably thermostable, half of its activity remains after 0.7 h at 90°C | Thermoclostridium stercorarium |
85 | - |
recombinant arfB gene product, stable up to | Thermoclostridium stercorarium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Aspergillus niger |
5 | - |
- |
Thermoclostridium stercorarium |