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Literature summary for 3.2.1.55 extracted from
- Purification and characterization of alpha-L-arabinofuranosidase from Bacillus stearothermophilus T-6 (1995), Appl. Environ. Microbiol., 61, 170-174.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| L-arabinose | inducer of AF activity | Butyrivibrio fibrisolvens |  |
| L-arabinose | inducer of AF activity | Trichoderma reesei | |
| xylan | induces enzyme activity | Butyrivibrio fibrisolvens | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Streptomyces lividans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | 1 mM FeSO4 inhibits the activity by 25% | Geobacillus stearothermophilus | |
| Hg2+ | 1 mM HgCl2 inhibits the activity by 98% | Geobacillus stearothermophilus | |
| Hg2+ | - |
Streptomyces purpurascens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.42 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Geobacillus stearothermophilus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Geobacillus stearothermophilus | 5737 | - |
| cytoplasm | - |
Butyrivibrio fibrisolvens | 5737 | - |
| cytoplasm | - |
Streptomyces lividans | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | weak stimulation of the enzyme activity | Geobacillus stearothermophilus | |
| Mg2+ | small stimulating effect | Geobacillus stearothermophilus | |
| Mn2+ | small stimulating effect | Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
4 * 64000, SDS-PAGE | Geobacillus stearothermophilus |
| 256000 | - |
- |
Geobacillus stearothermophilus |
| 284000 | - |
gel filtration | Geobacillus stearothermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beet arabinan + H2O | Geobacillus stearothermophilus | - |
L-arabinose + ? | - |
? | |
| beet arabinan + H2O | Aspergillus niger | - |
L-arabinose + ? | - |
? | |
| beet arabinan + H2O | Ruminococcus albus | - |
L-arabinose + ? | - |
? | |
| beet arabinan + H2O | Butyrivibrio fibrisolvens | - |
L-arabinose + ? | - |
? | |
| beet arabinan + H2O | Clostridium acetobutylicum | - |
L-arabinose + ? | - |
? | |
| beet arabinan + H2O | Streptomyces lividans | - |
L-arabinose + ? | - |
? | |
| beet arabinan + H2O | Streptomyces purpurascens | - |
L-arabinose + ? | - |
? | |
| beet arabinan + H2O | Ruminococcus albus 8 | - |
L-arabinose + ? | - |
? | |
| beet arabinan + H2O | Butyrivibrio fibrisolvens GS113 | - |
L-arabinose + ? | - |
? | |
| beet arabinan + H2O | Streptomyces lividans 66 | - |
L-arabinose + ? | - |
? | |
| beet arabinan + H2O | Streptomyces lividans 66 1326 | - |
L-arabinose + ? | - |
? | |
| beet arabinan + H2O | Geobacillus stearothermophilus L1 | - |
L-arabinose + ? | - |
? | |
| beet arabinan + H2O | Geobacillus stearothermophilus T-6 | - |
L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | Geobacillus stearothermophilus | - |
L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | Aspergillus niger | - |
L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | Butyrivibrio fibrisolvens | - |
L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | Clostridium acetobutylicum | - |
L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | Trichoderma reesei | - |
L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | Streptomyces diastaticus | - |
L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | Butyrivibrio fibrisolvens GS113 | - |
L-arabinose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
| Butyrivibrio fibrisolvens | - |
GS113 | - |
| Butyrivibrio fibrisolvens GS113 | - |
GS113 | - |
| Clostridium acetobutylicum | - |
ATCC 824 | - |
| Geobacillus stearothermophilus | - |
L1 | - |
| Geobacillus stearothermophilus | - |
T-6 | - |
| Geobacillus stearothermophilus L1 | - |
L1 | - |
| Geobacillus stearothermophilus T-6 | - |
T-6 | - |
| Ruminococcus albus | - |
8 | - |
| Ruminococcus albus 8 | - |
8 | - |
| Sclerotinia sclerotiorum | - |
- |
- |
| Streptomyces diastaticus | - |
- |
- |
| Streptomyces lividans | - |
66 | - |
| Streptomyces lividans | - |
66strain 1326 | - |
| Streptomyces lividans 66 | - |
66 | - |
| Streptomyces lividans 66 1326 | - |
66strain 1326 | - |
| Streptomyces purpurascens | - |
IFO 3389 | - |
| Trichoderma reesei | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
- |
Ruminococcus albus |
- |
Butyrivibrio fibrisolvens |
- |
Streptomyces lividans |
- |
Streptomyces diastaticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 95 | - |
extracellular enzyme activity | Geobacillus stearothermophilus |
| 95 | - |
extracellular enzyme activity | Butyrivibrio fibrisolvens |
| 749.1 | - |
- |
Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beet arabinan + H2O | - |
Geobacillus stearothermophilus | L-arabinose + ? | - |
? | |
| beet arabinan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
? | |
| beet arabinan + H2O | - |
Ruminococcus albus | L-arabinose + ? | - |
? | |
| beet arabinan + H2O | - |
Butyrivibrio fibrisolvens | L-arabinose + ? | - |
? | |
| beet arabinan + H2O | - |
Clostridium acetobutylicum | L-arabinose + ? | - |
? | |
| beet arabinan + H2O | - |
Streptomyces lividans | L-arabinose + ? | - |
? | |
| beet arabinan + H2O | - |
Streptomyces purpurascens | L-arabinose + ? | - |
? | |
| beet arabinan + H2O | - |
Ruminococcus albus 8 | L-arabinose + ? | - |
? | |
| beet arabinan + H2O | - |
Butyrivibrio fibrisolvens GS113 | L-arabinose + ? | - |
? | |
| beet arabinan + H2O | - |
Streptomyces lividans 66 | L-arabinose + ? | - |
? | |
| beet arabinan + H2O | - |
Streptomyces lividans 66 1326 | L-arabinose + ? | - |
? | |
| beet arabinan + H2O | - |
Geobacillus stearothermophilus L1 | L-arabinose + ? | - |
? | |
| beet arabinan + H2O | - |
Geobacillus stearothermophilus T-6 | L-arabinose + ? | - |
? | |
| carboxymethyl cellulose + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
| carboxymethyl cellulose + H2O | - |
Geobacillus stearothermophilus L1 | ? | - |
? | |
| carboxymethyl cellulose + H2O | - |
Geobacillus stearothermophilus T-6 | ? | - |
? | |
| L-arabinan + H2O | - |
Geobacillus stearothermophilus | (1,5-alpha-L-arabinose)25 + ? | - |
? | |
| L-arabinan + H2O | - |
Aspergillus niger | (1,5-alpha-L-arabinose)25 + ? | - |
? | |
| L-arabinan + H2O | - |
Clostridium acetobutylicum | (1,5-alpha-L-arabinose)25 + ? | - |
? | |
| L-arabinan + H2O | - |
Streptomyces lividans | (1,5-alpha-L-arabinose)25 + ? | - |
? | |
| L-arabinan + H2O | - |
Streptomyces purpurascens | (1,5-alpha-L-arabinose)25 + ? | - |
? | |
| L-arabinan + H2O | - |
Streptomyces lividans 66 | (1,5-alpha-L-arabinose)25 + ? | - |
? | |
| L-arabinan + H2O | - |
Streptomyces lividans 66 1326 | (1,5-alpha-L-arabinose)25 + ? | - |
? | |
| L-arabinan + H2O | - |
Geobacillus stearothermophilus L1 | (1,5-alpha-L-arabinose)25 + ? | - |
? | |
| L-arabinan + H2O | - |
Geobacillus stearothermophilus T-6 | (1,5-alpha-L-arabinose)25 + ? | - |
? | |
| additional information | - |
Aspergillus niger | ? | - |
? | |
| additional information | - |
Butyrivibrio fibrisolvens | ? | - |
? | |
| additional information | - |
Clostridium acetobutylicum | ? | - |
? | |
| additional information | - |
Streptomyces lividans | ? | - |
? | |
| additional information | - |
Streptomyces purpurascens | ? | - |
? | |
| additional information | no activity detected with beta-L-arabinopyranoside, beta-D-cellobiose, alpha-L-fucopyranoside, beta-L-fucopyranoside, alpha-L-rhamnopyranoside, alpha-L-arabinopyranoside, beta-D-galactopyranoside, beta-D-glucopyranoside, alpha-D-mannopyranoside, beta-D-xylopyranoside and arabinogalactan | Geobacillus stearothermophilus | ? | - |
? | |
| additional information | - |
Butyrivibrio fibrisolvens GS113 | ? | - |
? | |
| additional information | - |
Streptomyces lividans 66 | ? | - |
? | |
| additional information | - |
Streptomyces lividans 66 1326 | ? | - |
? | |
| additional information | no activity detected with beta-L-arabinopyranoside, beta-D-cellobiose, alpha-L-fucopyranoside, beta-L-fucopyranoside, alpha-L-rhamnopyranoside, alpha-L-arabinopyranoside, beta-D-galactopyranoside, beta-D-glucopyranoside, alpha-D-mannopyranoside, beta-D-xylopyranoside and arabinogalactan | Geobacillus stearothermophilus L1 | ? | - |
? | |
| additional information | no activity detected with beta-L-arabinopyranoside, beta-D-cellobiose, alpha-L-fucopyranoside, beta-L-fucopyranoside, alpha-L-rhamnopyranoside, alpha-L-arabinopyranoside, beta-D-galactopyranoside, beta-D-glucopyranoside, alpha-D-mannopyranoside, beta-D-xylopyranoside and arabinogalactan | Geobacillus stearothermophilus T-6 | ? | - |
? | |
| oat spelt xylan + H2O | - |
Geobacillus stearothermophilus | L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | - |
Butyrivibrio fibrisolvens | L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | - |
Clostridium acetobutylicum | L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | - |
Trichoderma reesei | L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | - |
Streptomyces diastaticus | L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | - |
Butyrivibrio fibrisolvens GS113 | L-arabinose + ? | - |
? | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Geobacillus stearothermophilus | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Ruminococcus albus | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Butyrivibrio fibrisolvens | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Sclerotinia sclerotiorum | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Clostridium acetobutylicum | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Trichoderma reesei | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces lividans | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces purpurascens | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces diastaticus | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Ruminococcus albus 8 | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Butyrivibrio fibrisolvens GS113 | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces lividans 66 | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces lividans 66 1326 | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Geobacillus stearothermophilus L1 | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Geobacillus stearothermophilus T-6 | L-arabinose + phenol | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | strain L1 | Geobacillus stearothermophilus |
| octamer | - |
Butyrivibrio fibrisolvens |
| octamer | - |
Streptomyces purpurascens |
| tetramer | - |
Ruminococcus albus |
| tetramer | 4 * 64000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Geobacillus stearothermophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 80 | - |
Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6 | - |
Geobacillus stearothermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | - |
Geobacillus stearothermophilus |
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