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Literature summary for 3.2.1.55 extracted from
- An alpha-L-arabinofuranosidase from Trichoderma reesei containing a noncatalytic xylan-binding domain (1999), Appl. Environ. Microbiol., 65, 3964-3968.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| L-arabinose | inducer of AF activity | Trichoderma reesei |  |
| sorbose | inducer of AF activity | Trichoderma reesei | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Streptomyces lividans |
| alpha-AF cDNA cloned from Trichoderma reesei RUT C-30 chromosomal DNA using PCR technique, trying to express this gene in the yeast Schizosaccharomyces pombe, expression in Saccharomyces cerevisiae | Trichoderma reesei |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.9 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | isoenzyme AF53 | Trichoderma reesei | |
| 1.1 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | isoenzyme AF35 | Trichoderma reesei | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
AF35, SDS-PAGE | Trichoderma reesei |
| 53000 | - |
AF53, SDS-PAGE | Trichoderma reesei |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oat spelt xylan + H2O | Trichoderma reesei | - |
L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | Trichoderma reesei PC-3-7 | - |
L-arabinose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces lividans | - |
66strain 1326 | - |
| Streptomyces lividans 66 1326 | - |
66strain 1326 | - |
| Trichoderma reesei | - |
PC-3-7 | - |
| Trichoderma reesei PC-3-7 | - |
PC-3-7 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isoenzymes AF35 and AF53 | Trichoderma reesei |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| conidium | - |
Trichoderma reesei | - |
| mycelium | - |
Trichoderma reesei | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.061 | - |
isoenzyme AF35, oat spelt xylan as substrate | Trichoderma reesei |
| 2.6 | - |
isoenzyme AF53, oat spelt xylan as substrate | Trichoderma reesei |
| 120 | - |
isoenzyme AF53 | Trichoderma reesei |
| 196 | - |
isoenzyme AF35 | Trichoderma reesei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arabinoxylan + H2O | - |
Trichoderma reesei | L-arabinose + ? | - |
ir | |
| L-arabinoxylan + H2O | - |
Streptomyces lividans | L-arabinose + ? | - |
ir | |
| L-arabinoxylan + H2O | - |
Trichoderma reesei PC-3-7 | L-arabinose + ? | - |
ir | |
| L-arabinoxylan + H2O | - |
Streptomyces lividans 66 1326 | L-arabinose + ? | - |
ir | |
| oat spelt xylan + H2O | - |
Trichoderma reesei | L-arabinose + ? | - |
? | |
| oat spelt xylan + H2O | - |
Trichoderma reesei PC-3-7 | L-arabinose + ? | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Trichoderma reesei | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Streptomyces lividans | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Trichoderma reesei PC-3-7 | p-nitrophenol + L-arabinose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Streptomyces lividans 66 1326 | p-nitrophenol + L-arabinose | - |
? | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Trichoderma reesei | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces lividans | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Trichoderma reesei PC-3-7 | L-arabinose + phenol | - |
ir | |
| phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces lividans 66 1326 | L-arabinose + phenol | - |
ir | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Trichoderma reesei |
| 60 | - |
- |
Streptomyces lividans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
stable up to | Trichoderma reesei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Trichoderma reesei |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | isoenzyme AF35 | Trichoderma reesei |
| 4.5 | 7 | isoenzyme AF53 | Trichoderma reesei |
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